Refolding Record:
| Protein | |
|---|---|
| Protein Name | Elongation factor G |
| Abbreviated Name | EF-G |
| SCOP Family | Unknown |
| Structure Notes | |
| Organism | Staphylococcus aureus |
| UniProt Accession | P68789 |
| SCOP Unique ID | n/a |
| Structure Solved | |
| Class | Unknown |
| Molecularity | Unknown |
| Construct | |
|---|---|
| Full Length | y |
| Domain | n/a |
| Chimera | n/a |
| Variants | n/a |
| Chain Length | 692 |
| Molecular Weight | 76481.2 |
| Pi | 4.79 |
| Molecular Weight | 76481.2 |
| Disulphides | Unknown |
| Full Sequence |
AREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGE
|
| Notes | n/a |
| Expression | |
|---|---|
| Report | Li JJ, Venkataramana M, Sanyal S, Janson JC, Su ZG. (2006) Protein Expression and Purification, 45, 72-9 |
| Project Aim | Protein refolding |
| Fusion | None |
| Protein Expression and Production | Protein recombinantly expressed as and refolded from inclusion bodies. |
| Expression Host | Escherichia coli |
| Expression Strain | BL21(DE3) |
| Expression Temp | 30.0 |
| Expression Time | 00 |
| Expression Vector | pET30 |
| Expression Protocol | The pET30 plasmid containing S. aureus fusA insert was transformed into E. coli BL21 (DE3) for over-expression of EF-G protein. The transformed cells were grown in LB containing 50 μg/ml kanamycin at 30 °C and was induced with 1 mM IPTG at OD595 of 0.4–0.5. The over-expressed protein was found mostly in inclusion body (80%) and the rest was in soluble form (20%). The soluble fraction was purified using a His-trap Ni-chelating column and was used as reference for the protein refolded from inclusion body. |
| Method of Induction | IPTG |
| Cell Density at Induction | OD 0.5-0.6 = 595 |
| Cell Disruption Method | French Press |
| Lytic Agent | None |
| Pre-Refolding Purification | not specified |
| Solubility | partial |
| Refolding | |
|---|---|
| Refolding Method | Dilution/ chaperone-assisted refolding |
| Wash Buffer | 50 mM Tris, pH 8.5, containing 1% Triton X-100 and 1 mM EDTA |
| Solubilization Buffer | 50 mM Tris, pH 8.7, containing 6 M Gu–HCl, 1 mM EDTA, and 150 mM DTT |
| Refolding Buffer | 50 mM Tris, pH 8.6, containing 5 mM GSH, 0.5 mM GSSG, 3% (W/V) B-CD (β-Cyclodextrin), 3% (w/v) b-CD polymer |
| Pre-Refolding Purification | not specified |
| Tag Cleaved | no tag |
| Refolding pH | 8.6 |
| Refolding Temperature | 0.0 |
| Protein Concentration | n/a |
| Refolding Time | n/a |
| Redox Agent | GSH/GSSG |
| Redox Agent Concentration | 5/0.5 mM |
| Refolding Protocol | For soluble artificial chaperone-assisted refolding, 50 μl unfolded EF-G described above was mixed with 50 μl of 10% Triton X-100 and was 20- and 50-fold diluted by the refolding buffer followed by the addition of 3% (w/v) β-CD and 3% (w/v) β-CD polymer, respectively. |
| Refolding Assay | Fluorescence,RP-HPLC,Circular Dichroism (uv-CD) |
| Refolding Chaperones | None |
| Refolding Additives | β-cyclodextrin,β-Cyclodextrin polymer |
| Additives Concentration | 3%/3% w/v |
| Refolding Yield | 99% |
| Purity | n/a |
| Notes | n/a |