Refolding Record:
| Protein | |
|---|---|
| Protein Name | Testis expressed transcript protein |
| Abbreviated Name | BJ-HCC-2 protein |
| SCOP Family | Unknown |
| Structure Notes | |
| Organism | Human |
| UniProt Accession | Q969F0 |
| SCOP Unique ID | n/a |
| Structure Solved | |
| Class | Unknown |
| Molecularity | Monomer |
| Construct | |
|---|---|
| Full Length | y |
| Domain | n/a |
| Chimera | n/a |
| Variants | n/a |
| Chain Length | 186 |
| Molecular Weight | 20711.6 |
| Pi | 9.51376 |
| Molecular Weight | 20711.6 |
| Disulphides | Unknown |
| Full Sequence |
MAGGPPNTKAEMEMSLAEELNHGRQGENQEHLVIAEMMELGSRSRGASQKKQKLEQKAAG
SASAKRVWNMTATRPKKMGSQLPKPRMLRESGHGDAHLQEYAGNFQGIRFHYDRNPGTDA
VAQTSLEEFNVLEMEVMRRQLYAVNRRLRALEEQGATWRHRETLIIAVLVSASIANLWLW
MNQ
|
| Notes | n/a |
| Expression | |
|---|---|
| Report | Yang XA, Dong XY, Li Y, Wang YD, Chen WF. (2004) Protein Expression and Purification, 33, 332-338 |
| Project Aim | Structure-Function |
| Fusion | None |
| Protein Expression and Production | Protein recombinantly expressed as and refolded from inclusion bodies. |
| Expression Host | Escherichia coli |
| Expression Strain | M15 |
| Expression Temp | 37.0 |
| Expression Time | 6h |
| Expression Vector | pQE30/BJ-HCC-2 |
| Expression Protocol | unknown |
| Method of Induction | Not Stated |
| Cell Density at Induction | OD = |
| Cell Disruption Method | Sonication |
| Lytic Agent | None |
| Pre-Refolding Purification | None |
| Solubility | insoluble |
| Refolding | |
|---|---|
| Refolding Method | Dialysis |
| Wash Buffer | 20 mM Tris?HCl, 5 mM EDTA, and 1% Triton X-100 |
| Solubilization Buffer | 0.3% N-lauroyl sarcosine, 50 mM CAPS buffer, and 0.3 M NaCl |
| Refolding Buffer | 20 mM Tris?HCl buffer, 10% glycerol |
| Pre-Refolding Purification | None |
| Tag Cleaved | yes |
| Refolding pH | 8.0 |
| Refolding Temperature | 37.0 |
| Protein Concentration | 0.46mg/ml |
| Refolding Time | 24h |
| Redox Agent | None |
| Redox Agent Concentration | n/a |
| Refolding Protocol | The cell pellet was resuspended in sonic buffer (20 mM Tris? HCl, 1 mM EDTA, 0.3 M NaCl, and 1 mg/ml lysozyme, pH 8.0) for 30 min at 4 |
| Refolding Assay | Far-UV Circular Dichroism |
| Refolding Chaperones | None |
| Refolding Additives | None |
| Additives Concentration | NULL |
| Refolding Yield | 71.7% |
| Purity | 97% |
| Notes | REFOLDING TEMPERATURE WAS ENTERED AS 0 DEGREES BUT ARBITRARILY CHANGED TO 37 DEGREES - JASON |