Refolding Record:
| Construct | |
|---|---|
| Full Length | y |
| Domain | n/a |
| Chimera | n/a |
| Variants | n/a |
| Chain Length | 260 |
| Molecular Weight | 29113.8 |
| Pi | 6.40522 |
| Molecular Weight | 29113.8 |
| Disulphides | Unknown |
| Full Sequence |
MSHHWGYGKH NGPEHWHKDF PIANGERQSP VDIDTKAVVQ DPALKPLALV YGEATSRRMV NNGHSFNVEY DDSQDKAVLK DGPLTGTYRL VQFHFHWGSS DDQGSEHTVD RKKYAAELHL VHWNTKYGDF GTAAQQPDGL AVVGVFLKVG DANPALQKVL DALDSIKTKG KSTDFPNFDP GSLLPNVLDY WTYPGSLTTP PLLESVTWIV LKEPISVSSQ QMLKFRTLNF NAEGEPELLM LANWRPAQPL KNRQVRGFPK
|
| Notes | n/a |
| Expression | |
|---|---|
| Report | Unpublished (0) J Virol Methods, 0, 0 |
| Project Aim | Folding |
| Fusion | None |
| Protein Expression and Production | Protein expressed and purified in native conformation prior to denaturation and refolding. |
| Expression Host | |
| Expression Strain | |
| Expression Temp | 0.0 |
| Expression Time | 0 |
| Expression Vector | |
| Expression Protocol | |
| Method of Induction | Not Stated |
| Cell Density at Induction | OD = |
| Cell Disruption Method | None |
| Lytic Agent | None |
| Pre-Refolding Purification | None |
| Solubility | |
| Refolding | |
|---|---|
| Refolding Method | Dialysis |
| Wash Buffer | None |
| Solubilization Buffer | 8 M urea in 50 mM phosphate, pH 8, Schiff reagents from Boston Protein Solutions |
| Refolding Buffer | Tris, 50 mM, pH 8 |
| Pre-Refolding Purification | None |
| Tag Cleaved | no tag |
| Refolding pH | 8.0 |
| Refolding Temperature | 4.0 |
| Protein Concentration | 1.0 mg/ml |
| Refolding Time | 20 hours |
| Redox Agent | None |
| Redox Agent Concentration | n/a |
| Refolding Protocol | None |
| Refolding Assay | Activity assay |
| Refolding Chaperones | none |
| Refolding Additives | Schiff base reagents from Boston Protein Solutions |
| Additives Concentration | 20 mM |
| Refolding Yield | 81% |
| Purity | n/a |
| Notes | n/a |