Refolding Record:
| Protein | |
|---|---|
| Protein Name | CLIPB14 |
| Abbreviated Name | CLIPB14 |
| SCOP Family | Eukaryotic Proteases |
| Structure Notes | |
| Organism | Anopheles gambiae |
| UniProt Accession | Q7QGN4 |
| SCOP Unique ID | n/a |
| Structure Solved | |
| Class | Beta |
| Molecularity | Monomer |
| Construct | |
|---|---|
| Full Length | y |
| Domain | n/a |
| Chimera | n/a |
| Variants | n/a |
| Chain Length | 375 |
| Molecular Weight | 41117.0 |
| Pi | 7.82763 |
| Molecular Weight | 41117.0 |
| Disulphides | 7 |
| Full Sequence |
MYSRRYVACGLLCLLVIAIDQGHGQEHKPCTTPNGTAGRCVRVRECGYVLDLLRKDLFAHSDTVHLEGLQCGTRPDGGALVCCPAFVNEPNCGPSVFGVRIIGGNDTELGEFPWMALLRFQARNRKIHGNCGASLVSKRFVLSAAHCFTAAKSKGWKIHSVRVAEWNFMNHRGSKDCKQVKGYDVPICRKDYDVARFVQHPEYRVNAGVHVNDIVLIELAADVEYNVFVAPICLPVSNDTAQLPWGSSDDPEIEYTAAGWGSTESGKESTGMSYQLKQINLRAFNKERCKKLFQVPSGVGVGLGHICAGGIRDEDTCHGDSGGPLMEAVGGVWYLAGITSFGWPRCGRDGVPGVYTNISHYMGWLEREMFRGILA
|
| Notes | n/a |
| Expression | |
|---|---|
| Report | Schrödel A, Volz J, de Marco A (2005) J Biotechnology, 120, 2-10 |
| Project Aim | Functional Studies |
| Fusion | Ftr |
| Protein Expression and Production | Protein recombinantly expressed as and refolded from inclusion bodies. |
| Expression Host | Escherichia coli |
| Expression Strain | BL21 |
| Expression Temp | 20.0 |
| Expression Time | 18 hours |
| Expression Vector | pETM90 |
| Expression Protocol | No protocol recorded |
| Method of Induction | Not Stated |
| Cell Density at Induction | OD = |
| Cell Disruption Method | Sonication |
| Lytic Agent | Lysozyme |
| Pre-Refolding Purification | Washing inclusion body |
| Solubility | insoluble |
| Refolding | |
|---|---|
| Refolding Method | Dilution |
| Wash Buffer | urea 9M |
| Solubilization Buffer | urea 9M |
| Refolding Buffer | 55 mM MES, pH6.5, 0.45 KCl, 2.2 MgCl, 440 mM Sucrose |
| Pre-Refolding Purification | Washing inclusion body |
| Tag Cleaved | no |
| Refolding pH | 7.5 |
| Refolding Temperature | 25.0 |
| Protein Concentration | |
| Refolding Time | |
| Redox Agent | GSH/GSSG |
| Redox Agent Concentration | n/a,n/a,n/a |
| Refolding Protocol | FoldIt Screen kit instruction |
| Refolding Assay | SDS-PAGE |
| Refolding Chaperones | None |
| Refolding Additives | None |
| Additives Concentration | NULL |
| Refolding Yield | |
| Purity | |
| Notes | chimera |