Refolding Record:
| Protein | |
|---|---|
| Protein Name | Major allergen Asp F1 |
| Abbreviated Name | Asp f I/a |
| SCOP Family | Unknown |
| Structure Notes | |
| Organism | Aspergillus fumigatus |
| UniProt Accession | P67875 |
| SCOP Unique ID | n/a |
| Structure Solved | |
| Class | Unknown |
| Molecularity | Unknown |
| Construct | |
|---|---|
| Full Length | y |
| Domain | n/a |
| Chimera | n/a |
| Variants | n/a |
| Chain Length | 160 |
| Molecular Weight | 18121.2 |
| Pi | 9.24641 |
| Molecular Weight | 18121.2 |
| Disulphides | Unknown |
| Full Sequence |
MRGSHHHHHHGS ATW TCINQQLNPK TNKWEDKRLL YSQAKAESNS HHAPLSDGKT GSSYPHWFTN GYDGNGKLIK GRTPIKFGKA DCDRPPKHSQ NGMGKDDHYL LEFPTFPDGH DYKFDSKKPK EDPGPARVIY TYPNKVFCGI VAHQRGNQGD LRLCS
|
| Notes | n/a |
| Expression | |
|---|---|
| Report | Moser M, Crameri R, Menz G, Schneider T, Dudler T, Virchow C, Gmachl M, Blaser K, Suter M (1992) Journal of Immunology, 149, 454-460 |
| Project Aim | Drug Studies |
| Fusion | N-terminal hexahistidine tag |
| Protein Expression and Production | Protein recombinantly expressed as and refolded from inclusion bodies. |
| Expression Host | Escherichia coli |
| Expression Strain | M15 |
| Expression Temp | 37.0 |
| Expression Time | not stated |
| Expression Vector | p6xHisDHFRS |
| Expression Protocol | no details provided |
| Method of Induction | Not Stated |
| Cell Density at Induction | OD = |
| Cell Disruption Method | None |
| Lytic Agent | None |
| Pre-Refolding Purification | Affinity chromotography |
| Solubility | insoluble |
| Refolding | |
|---|---|
| Refolding Method | Dialysis |
| Wash Buffer | not stated |
| Solubilization Buffer | not stated |
| Refolding Buffer | PBS pH 7.4 |
| Pre-Refolding Purification | Affinity chromotography |
| Tag Cleaved | no |
| Refolding pH | 7.4 |
| Refolding Temperature | 4.0 |
| Protein Concentration | |
| Refolding Time | 2 days |
| Redox Agent | None |
| Redox Agent Concentration | n/a |
| Refolding Protocol | For refolding, the protein was dialysed against refolding buffer for 2 days at 4degC. |
| Refolding Assay | Enzyme activity |
| Refolding Chaperones | None |
| Refolding Additives | None |
| Additives Concentration | |
| Refolding Yield | |
| Purity | |
| Notes | |