Refolding Record:
| Protein | |
|---|---|
| Protein Name | chemoattractive glycoprotein ES20 |
| Abbreviated Name | ES20 |
| SCOP Family | chemoattractive glycoprotein |
| Structure Notes | |
| Organism | Lumbricus terrestris |
| UniProt Accession | O44335 |
| SCOP Unique ID | n/a |
| Structure Solved | |
| Class | Unknown |
| Molecularity | Unknown |
| Construct | |
|---|---|
| Full Length | y |
| Domain | n/a |
| Chimera | n/a |
| Variants | n/a |
| Chain Length | 212 |
| Molecular Weight | 23385.8 |
| Pi | 6.06054 |
| Molecular Weight | 23385.8 |
| Disulphides | Unknown |
| Full Sequence |
mrgsmktylllvflvgahalvcppgftylpagescykvifeshdwhsatercrqesrglaaistpeesiavkefidteiskdsagaavchptgqsgirfwtsglqtkdtctktsfllkitntfevpfdftnwadgeptlprktekfsalivgssertpsgttmtatsscvhsanisndtlkrisvlphlyvgfcdeiwlylnfclisiqili
|
| Notes | n/a |
| Expression | |
|---|---|
| Report | Liu W, Wang D, Chen P, Halpern M. (1997) J Biol Chem, 272, 27378-27381 |
| Project Aim | Structure-Function |
| Fusion | N-terminal hexahistidine tag |
| Protein Expression and Production | Protein recombinantly expressed as and refolded from inclusion bodies. |
| Expression Host | Escherichia coli |
| Expression Strain | M15[pREP4] |
| Expression Temp | 37.0 |
| Expression Time | 3hrs |
| Expression Vector | pQE30 |
| Expression Protocol | As stated in the manuscript "The purified protein was refolded by dialyzing against stepwise dilutions of the denaturant. " |
| Method of Induction | IPTG |
| Cell Density at Induction | OD n/a = n/a |
| Cell Disruption Method | Not stated |
| Lytic Agent | None |
| Pre-Refolding Purification | Metal affinity chromatography |
| Solubility | insoluble |
| Refolding | |
|---|---|
| Refolding Method | Dialysis |
| Wash Buffer | Not stated |
| Solubilization Buffer | Not stated |
| Refolding Buffer | Not stated |
| Pre-Refolding Purification | Metal affinity chromatography |
| Tag Cleaved | no |
| Refolding pH | 7.4 |
| Refolding Temperature | 4.0 |
| Protein Concentration | not stated |
| Refolding Time | not stated |
| Redox Agent | None |
| Redox Agent Concentration | n/a |
| Refolding Protocol | The purified protein was refolded by dialyzing against stepwise dilutions of the denaturant. |
| Refolding Assay | Bioactivity |
| Refolding Chaperones | None |
| Refolding Additives | None |
| Additives Concentration | n/a |
| Refolding Yield | n/a |
| Purity | n/a |
| Notes | n/a |