Refolding Records
Protein | Class | Organism | Family | Molecularity | MW (Da) | Refolding Method | Redox Agent |
---|---|---|---|---|---|---|---|
Der f 1 | Unknown | Dermatophagoides farinae (dust mite) | Unknown | Unknown | 34644.7 | Column refolding: Size-exclusion chromatography | None |
Dihydrofolate reductase-thymidylate synthase | Alpha/Beta | Plasmodium falciparum | Dihydrofolate reductases | Monomer | 27103.5 | Dilution | DTT |
Interleukin-6 | Alpha | Human | Long-Chain Cytokines | Monomer | 16585.8 | Dialysis | None |
Chitin deacetylase | Unknown | Colletotrichum lindemuthianum | Unknown | Unknown | 27808.0 | Dilution | None |
Vascular endothelial growth factor | Small Proteins | Human | Platelet-derived growth factor-like | Unknown | 14940.0 | Dilution | None |
Vascular endothelial growth factor | Small Proteins | Human | Platelet-derived growth factor-like | Unknown | 19226.0 | Dilution | None |
Insulin-like growth factor binding protein 3 | Unknown | Human | Unknown | Unknown | 15093.1 | Dilution | DTT/cystamine |
Early protein GP1 | Unknown | Bacteriophage phi-29 | Unknown | Unknown | 10715.3 | Column refolding: Nickel-chelating chromatography | None |
Haemoglobin alpha subunit | Alpha | Human | Globins | Monomer | 15126.4 | Dilution | None |
Growth Hormone | Alpha | Human | Long-Chain Cytokines | Monomer | 24847.3 | Dialysis | Cysteine |
Cytomegalovirus protease | Beta | Human cytomegalovirus | Herpes virus serine proteinase, assemblin | Monomer | 29433.9 | Dialysis | DTT |
HIV protease | Beta | HIV-1 | Retroviral protease (retropepsin) | Dimer | 13420.0 | Dilution | DTT |
Growth Hormone Receptor | Beta | Ovis aries (sheep/ovine) | Fibronectin type III | Monomer | 68958.4 | Dilution/Dialysis combination | Cysteine |
ADAMTS13 | Unknown | Human | Unknown | Unknown | 148313.0 | Dilution/Dialysis combination | GSH/GSSG |
Erythropoietin Receptor | Beta | Xenopus laevis | Fibronectin type III | Monomer | 56211.5 | Dialysis | DTT |
Macrophage colony-stimulating factor | Alpha | Human | Short Chain Cytokines | Dimer | 25134.4 | Dilution | GSH/GSSG |
Tissue-type plasminogen activator | Small Proteins | Human | Kringle Modules | Monomer | 39427.5 | Dialysis | GSH/GSSG |
Tissue-type plasminogen activator | Small Proteins | Human | Kringle Modules | Monomer | 39386.4 | Dialysis | Cysteine/Cystine |
Subtilisin BPN | Alpha/Beta | Bacillus amyloliquefaciens | Subtilases | Monomer | 39181.2 | Dialysis | None |
Lignin peroxidase | Alpha | Phanerochaete chrysosporium | CCP-like | Monomer | 39130.8 | Dialysis | GSSG |
Macrophage colony-stimulating factor | Alpha | Human | Short Chain Cytokines | Dimer | 17978.4 | Dilution | None |
Protein Kinase C | Small Proteins | Rat | Protein kinase cysteine-rich domain (cys2, phorbol-binding domain) | Monomer | 78357.7 | Dialysis | DTT |
Transforming growth factor beta 2 | Small Proteins | Human | Transforming growth factor (TGF)-beta | Dimer | 12719.6 | Dilution | Not specified |
Placental lactogen | Alpha | Bovine | Long-Chain Cytokines | Monomer | 21531.4 | Dilution | Cysteine |
RNA Dependent RNA Polymerase | Multi-domain proteins (alpha and beta) | Hepititis E Virus | RNA dependent RNA polymerases | Monomer | 43613.3 | Column refolding: Nickel-chelating chromatography | None |