REFOLDdb

List of publications referred to by REFOLDdb

1 Duarte Carlos A, Palomino Mickel Biotechnology and applied biochemistry. An improved method for purification and refolding of recombinant HIV Vif expressed in E. coli. 2017 Feb: https://www.ncbi.nlm.nih.gov/pubmed/28181316

2 Elleuche Skander, Krull Alina, Lorenz Ute, Antranikian Garabed The protein journal. Parallel N- and C-Terminal Truncations Facilitate Purification and Analysis of a 155-kDa Cold-Adapted Type-I Pullulanase. 2017 Feb: 36(1) 56-63 https://www.ncbi.nlm.nih.gov/pubmed/28176135

3 Taktak-BenAmar Awatef, Morjen Maram, Ben Mabrouk Hazem, Abdelmaksoud-Dammak Rania, Guerfali Mohamed, Fourati-Masmoudi Najla, Marrakchi Naziha, Gargouri Ali AMB Express. Expression, purification and functionality of bioactive recombinant human vascular endothelial growth factor VEGF165 in E. coli. 2017 Feb: 7(1) 33 https://www.ncbi.nlm.nih.gov/pubmed/28168572

4 Nazem Habib, Mohsenifar Afshin, Majdi Sahar Advanced biomedical research. Chitosan-myristate nanogel as an artificial chaperone protects neuroserpin from misfolding. 2016 Dec: 5 170 https://www.ncbi.nlm.nih.gov/pubmed/27995109

5 Rouse Sarah L, Hawthorne Wlliam J, Lambert Sebastian, Morgan Marc L, Hare Stephen A, Matthews Stephen Acta crystallographica. Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter. 2016 Dec: Section F, Structural biology communications. 72(Pt 12) 892-896 https://www.ncbi.nlm.nih.gov/pubmed/27917837

6 Wang Nan, Ren Kai, Jia Rong, Chen Wenting, Sun Ruirui BMC biotechnology. Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes. 2016 Dec: 16(1) 87 https://www.ncbi.nlm.nih.gov/pubmed/27908283

7 Ungelenk Sophia, Moayed Fatemeh, Ho Chi-Ting, Grousl Tomas, Scharf Annette, Mashaghi Alireza, Tans Sander, Mayer Matthias P, Mogk Axel, Bukau Bernd Nature communications. Small heat shock proteins sequester misfolding proteins in near-native conformation for cellular protection and efficient refolding. 2016 Nov: 7 13673 https://www.ncbi.nlm.nih.gov/pubmed/27901028

8 Sharma Prerna, Kaila Pallavi, Guptasarma Purnananda The FEBS journal. Creation of active TIM barrel enzymes through genetic fusion of half-barrel domain constructs derived from two distantly related glycosyl hydrolases. 2016 Oct: 283(23) 4340-4356 https://www.ncbi.nlm.nih.gov/pubmed/27749025

9 Sadavarte Rahul, Filipe Carlos D M, Ghosh Raja Biotechnology progress. Recovery of functionally-active protein from inclusion bodies using a thermal-cycling method. 2016 Sep: 33(1) 133-139 https://www.ncbi.nlm.nih.gov/pubmed/27690284

10 Bacchi Marine, Jullian Magali, Sirigu Serena, Fould Benjamin, Huet Tiphaine, Bruyand Lisa, Antoine Mathias, Vuillard Laurent, Ronga Luisa, Chavas Leonard M G, Nosjean Olivier, Ferry Gilles, Puget Karine, Boutin Jean A Protein science : a publication of the Protein Society. Total chemical synthesis, refolding, and crystallographic structure of fully active immunophilin calstabin 2 (FKBP12.6). 2016 Sep: 25(12) 2225-2242 https://www.ncbi.nlm.nih.gov/pubmed/27670942

11 Zhang Lichao, Li Zongwei, Shi Tonglin, La Xiaoqin, Li Hanqing, Li Zhuoyu BMC biotechnology. Design, purification and assessment of GRP78 binding peptide-linked Subunit A of Subtilase cytotoxic for targeting cancer cells. 2016 Sep: 16(1) 65 https://www.ncbi.nlm.nih.gov/pubmed/27585649

12 Gieseler Gesa, Pepelanova Iliyana, Stuckenberg Lena, Villain Louis, Nölle Volker, Odenthal Uwe, Beutel Sascha, Rinas Ursula, Scheper Thomas Applied microbiology and biotechnology. Purification of bone morphogenetic protein-2 from refolding mixtures using mixed-mode membrane chromatography. 2016 Aug: 101(1) 123-130 https://www.ncbi.nlm.nih.gov/pubmed/27542381

13 Jablonická Veronika, Mansfeld Johanna, Heilmann Ingo, Obložinský Marek, Heilmann Mareike Phytochemistry. Identification of a secretory phospholipase A2 from Papaver somniferum L. that transforms membrane phospholipids. 2016 Aug: 129 4-13 https://www.ncbi.nlm.nih.gov/pubmed/27473012

14 Lu Tsung Chi, Zhao Guan-Hao, Chen Yao Yun, Chien Chia-Ying, Huang Chi-Hung, Lin Kwang Hui, Chen Shen Liang Journal of Cancer. Transduction of Recombinant M3-p53-R12 Protein Enhances Human Leukemia Cell Apoptosis. 2016 Jul: 7(10) 1360-73 https://www.ncbi.nlm.nih.gov/pubmed/27390612

15 Faezi Sobhan, Bahrmand Ahmad Reza, Mahdavi Mehdi, Siadat Seyed Davar, Nikokar Iraj, Sardari Soroush, Holder Ian Alan International journal of molecular and cellular medicine. High Yield Overexpression, Refolding, Purification and Characterization of Pseudomonas aeruginosa Type B-Flagellin: An Improved Method Without Sonication. 2016 Jul: 5(1) 37-48 https://www.ncbi.nlm.nih.gov/pubmed/27386437

16 Baek Jong-Hyuk, Jung Juneyoung, Seo Jeongbin, Kim Jeong Hee, Kim Joungmok Journal of microbiology and biotechnology. Bacterial Overexpression and Denaturing Purification of VPS34-Binding Domain of Beclin 1. 2016 Jul: 26(10) 1808-1816 https://www.ncbi.nlm.nih.gov/pubmed/27363473

17 Qi Liang-Bo, Hu Li-Dan, Liu Huihui, Li Hai-Yun, Leng Xiao-Yao, Yan Yong-Bin Protein & cell. Cataract-causing mutation S228P promotes βB1-crystallin aggregation and degradation by separating two interacting loops in C-terminal domain. 2016 Jul: 7(7) 501-15 https://www.ncbi.nlm.nih.gov/pubmed/27318838

18 Katayama Yukie, Suzuki Tatsuya, Ebisawa Tatsuki, Ohtsuka Jun, Wang Shipeng, Natsume Ryo, Lo Yu-Hua, Senda Toshiya, Nagamine Toshihiro, Hull J Joe, Matsumoto Shogo, Nagasawa Hiromichi, Nagata Koji, Tanokura Masaru Biochimica et biophysica acta. A class-A GPCR solubilized under high hydrostatic pressure retains its ligand binding ability. 2016 Jul: 1858(9) 2145-51 https://www.ncbi.nlm.nih.gov/pubmed/27342372

19 Fan Jiying, Wang Zhanqing, Huang Liying, Shen Yaling Protein expression and purification. Efficient refolding of the bifunctional therapeutic fusion protein VAS-TRAIL by a triple agent solution. 2016 Jul: 125 68-73 https://www.ncbi.nlm.nih.gov/pubmed/26358405

20 Chen Liang-Hua, Cai Feng, Zhang Dan-Ju, Zhang Li, Zhu Peng, Gao Shun Biotechnology and applied biochemistry. Large-scale purification and characterization of recombinant human stem cell factor in Escherichia coli. 2016 Jun: https://www.ncbi.nlm.nih.gov/pubmed/27301759

21 Pouranvari Sara, Ebrahimi Firouz, Javadi Gholamreza, Maddah Bozorgmehr Iranian Red Crescent medical journal. Cloning, Expression, and Cost Effective Purification of Authentic Human Epidermal Growth Factor With High Activity. 2016 Jun: 18(3) e24966 https://www.ncbi.nlm.nih.gov/pubmed/27247796

22 Hao Lin, Zhang Zhi-Guo, Shi Zheng-Duo, Pang Kun, Zhang Jun-Jie, Dong Yang, Han Cong-Hui Cell biochemistry and biophysics. Optimization Studies on Prokaryotic Cell Expression of the Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL). 2016 Jun: 73(2) 275-9 https://www.ncbi.nlm.nih.gov/pubmed/25737132

23 Zhai Lu, Wu Ling, Li Feng, Burnham Robert S, Pizarro Juan C, Xu Bin Scientific reports. A Rapid Method for Refolding Cell Surface Receptors and Ligands. 2016 May: 6 26482 https://www.ncbi.nlm.nih.gov/pubmed/27215173

24 Chandrasekhar Saradha, Moorthy Balakrishnan S, Xie Ruichao, Topp Elizabeth M Pharmaceutical research. Thiol-Disulfide Exchange in Human Growth Hormone. 2016 May: 33(6) 1370-82 https://www.ncbi.nlm.nih.gov/pubmed/26887678

25 Zhang Ying, Bae Young-An, Zong Hong-Ying, Kong Yoon, Cai Guo-Bin Iranian journal of parasitology. Functionally Expression of Metalloproteinase in Taenia solium Metacestode and Its Evaluation for Serodiagnosis of Cysticercosis. 2016 Apr: 11(1) 35-45 https://www.ncbi.nlm.nih.gov/pubmed/27095967

26 Singh Alla, Ubaid-Ullah Shah, Ramteke Anup K, Batra Janendra K PloS one. Influence of Conformation of M. tuberculosis RNase P Protein Subunit on Its Function. 2016 Apr: 11(4) e0153798 https://www.ncbi.nlm.nih.gov/pubmed/27088505

27 Sang Ming, Zhang Jiaxin, Li Bin, Chen Yuqing Protein expression and purification. TRAIL-CM4 fusion protein shows in vitro antibacterial activity and a stronger antitumor activity than solo TRAIL protein. 2016 Apr: 122 82-9 https://www.ncbi.nlm.nih.gov/pubmed/26926590

28 Chen Ying, Wang Qi, Zhang Chun, Li Xiunan, Gao Qiang, Dong Changqing, Liu Yongdong, Su Zhiguo Protein expression and purification. Improving the refolding efficiency for proinsulin aspart inclusion body with optimized buffer compositions. 2016 Apr: 122 1-7 https://www.ncbi.nlm.nih.gov/pubmed/26826314

29 Wang B B, Li F C, Xu K Z, Ni M, Hu J S, Tian J H, Li Y Y, Shen W D, Li B Pesticide biochemistry and physiology. Effects of mutations on the structure and function of silkworm type 1 acetylcholinesterase. 2016 Mar: 129 1-6 https://www.ncbi.nlm.nih.gov/pubmed/27017875

30 Chen Xiaoshuang, Xu Huanli, Li Bo, Wang Feng, Chen Xiaoliang, Kong Dexin, Lin Xiukun Marine drugs. Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi. 2016 Mar: 14(3) https://www.ncbi.nlm.nih.gov/pubmed/27007382

31 Moghadam Malihe, Ganji Ali, Varasteh Abdolreza, Falak Reza, Sankian Mojtaba Reports of biochemistry & molecular biology. Refolding process of cysteine-rich proteins:Chitinase as a model. 2016 Mar: 4(1) 19-24 https://www.ncbi.nlm.nih.gov/pubmed/26989746

32 Sappakhaw Khomkrit, Takasila Ratchaneekorn, Sittikul Pichamon, Wattana-Amorn Pakorn, Assavalapsakul Wanchai, Boonyalai Nonlawat Molecular and biochemical parasitology. Biochemical characterization of plasmepsin V from Plasmodium vivax Thailand isolates: Substrate specificity and enzyme inhibition. 2016 Mar: 204(2) 51-63 https://www.ncbi.nlm.nih.gov/pubmed/26795263

33 Lixa Carolina, Marques Adriana F, Cortines Juliana R, Neves Bianca C, Oliveira Danielle M P, Anobom Cristiane D, Lima Luís Maurício T R, Pinheiro Anderson S Protein expression and purification. Refolding, purification, and preliminary structural characterization of the DNA-binding domain of the quorum sensing receptor RhlR from Pseudomonas aeruginosa. 2016 Mar: 121 31-40 https://www.ncbi.nlm.nih.gov/pubmed/26792557

34 Tian Haishan, Zhao Yang, Chen Nazi, Wu Meiyu, Gong Weiyue, Zheng Jie, Fernig David G, Jungbauer Alois, Wang Dezhong, Li Xiaokun, Jiang Chao Applied microbiology and biotechnology. High production in E. coli of biologically active recombinant human fibroblast growth factor 20 and its neuroprotective effects. 2016 Mar: 100(7) 3023-34 https://www.ncbi.nlm.nih.gov/pubmed/26603761

35 Dangi Arun Kumar, Rishi Praveen, Tewari Rupinder The protein journal. Enhancing the Yield of Active Recombinant Chitobiase by Physico-Chemical and In Vitro Refolding Studies. 2016 Feb: 35(1) 72-9 https://www.ncbi.nlm.nih.gov/pubmed/26831864

36 Tamaki Yukihiro, Harakuni Tetsuya, Yamaguchi Rui, Miyata Takeshi, Arakawa Takeshi Vaccine. Cholera toxin B subunit pentamer reassembled from Escherichia coli inclusion bodies for use in vaccination. 2016 Feb: 34(10) 1268-74 https://www.ncbi.nlm.nih.gov/pubmed/26828455

37 Hakiminia Forough, Khalifeh Khosrow, Sajedi Reza H, Ranjbar Bijan Spectrochimica acta. Determination of structural elements on the folding reaction of mnemiopsin by spectroscopic techniques. 2016 Feb: Part A, Molecular and biomolecular spectroscopy. 158 49-55 https://www.ncbi.nlm.nih.gov/pubmed/26803749

38 Vemula Sandeep, Vemula Sushma, Dedaniya Akshay, Ronda Srinivasa Reddy Analytical and bioanalytical chemistry. In vitro refolding with simultaneous purification of recombinant human parathyroid hormone (rhPTH 1-34) from Escherichia coli directed by protein folding size exclusion chromatography (PF-SEC): implication of solution additives and their role on aggregates and renaturation. 2016 Feb: 408(1) 217-29 https://www.ncbi.nlm.nih.gov/pubmed/26472319

39 Biter Amadeo B, de la Peña Andres H, Thapar Roopa, Lin Jean Z, Phillips Kevin J Scientific reports. DSF Guided Refolding As A Novel Method Of Protein Production. 2016 Jan: 6 18906 https://www.ncbi.nlm.nih.gov/pubmed/26783150

40 Liu Hu, Dong Xiaoyan, Sun Yan Journal of chromatography. Grafting iminodiacetic acid on silica nanoparticles for facilitated refolding of like-charged protein and its metal-chelate affinity purification. 2016 Jan: A. 1429 277-83 https://www.ncbi.nlm.nih.gov/pubmed/26755413

41 Chen Anqi, Zhang Li, Gu Shaohua, Tang Rong, Xie Yi, Ji Chaoneng Protein expression and purification. Investigation of TtrD, an expressing recombinant fusion tag, in Escherichia coli. 2016 Jan: 120 65-71 https://www.ncbi.nlm.nih.gov/pubmed/26690374

42 Sluchanko Nikolai N, Tugaeva Kristina V, Faletrov Yaroslav V, Levitsky Dmitrii I Protein expression and purification. High-yield soluble expression, purification and characterization of human steroidogenic acute regulatory protein (StAR) fused to a cleavable Maltose-Binding Protein (MBP). 2016 Jan: 119 27-35 https://www.ncbi.nlm.nih.gov/pubmed/26555181

43 Li Qiaojing, Richard Charles-Adrien, Moudjou Mohammed, Vidic Jasmina Journal of visualized experiments : JoVE. Purification and Refolding to Amyloid Fibrils of (His)6-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli. 2015 Dec:(106) e53432 https://www.ncbi.nlm.nih.gov/pubmed/26709825

44 Qu Zi-gang, Ma Xue-ting, Li Wen-hui, Zhang Nian-zhang, Yue Long, Cui Jian-min, Cai Jian-ping, Jia Wan-zhong, Fu Bao-quan Parasites & vectors. Molecular characterization of a cathepsin F-like protease in Trichinella spiralis. 2015 Dec: 8 652 https://www.ncbi.nlm.nih.gov/pubmed/26692208

45 Maurya Svetlana Rajkumar, Mahalakshmi Radhakrishnan The Journal of biological chemistry. N-helix and Cysteines Inter-regulate Human Mitochondrial VDAC-2 Function and Biochemistry. 2015 Dec: 290(51) 30240-52 https://www.ncbi.nlm.nih.gov/pubmed/26487717

46 Upadhyay Vaibhav, Singh Anupam, Panda Amulya K Protein expression and purification. Purification of recombinant ovalbumin from inclusion bodies of Escherichia coli. 2015 Dec: 117 52-8 https://www.ncbi.nlm.nih.gov/pubmed/26390939

47 Liu Peng, Robbins Arthur H, Marzahn Melissa R, McClung Scott H, Yowell Charles A, Stevens Stanley M, Dame John B, Dunn Ben M PloS one. Enzymatic Characterization of Recombinant Food Vacuole Plasmepsin 4 from the Rodent Malaria Parasite Plasmodium berghei. 2015 Oct: 10(10) e0141758 https://www.ncbi.nlm.nih.gov/pubmed/26510189

48 Hernychová L, Mrázek H, Ivanova L, Kukačka Z, Chmelík J, Novák P Physiological research / Academia Scientiarum Bohemoslovaca. Recombinant expression, in vitro refolding and characterizing disulfide bonds of a mouse inhibitory C-type lectin-like receptor Nkrp1b. 2015 Oct: 64 Suppl 1 S85-93 https://www.ncbi.nlm.nih.gov/pubmed/26447598

49 Jaenecke Frank, Friedrich-Epler Beatrice, Parthier Christoph, Stubbs Milton T Biochemistry. Membrane composition influences the activity of in vitro refolded human vitamin K epoxide reductase. 2015 Oct: 54(42) 6454-61 https://www.ncbi.nlm.nih.gov/pubmed/26435421

50 Kim Chang-Kyu, Lee Seung-Bae, Son Young-Jin Journal of microbiology and biotechnology. Large-Scale Refolding and Enzyme Reaction of Human Preproinsulin for Production of Human Insulin. 2015 Oct: 25(10) 1742-50 https://www.ncbi.nlm.nih.gov/pubmed/26139616

51 Zhang Liang, Morrison Anneliese J, Thibodeau Patrick H PloS one. Interdomain Contacts and the Stability of Serralysin Protease from Serratia marcescens. 2015 Sep: 10(9) e0138419 https://www.ncbi.nlm.nih.gov/pubmed/26378460

52 Jafari S, Babaeipour V, Seyedi H A Eslampanah, Rahaie M, Mofid M R, Haddad L, Namvaran M M, Fallah J Research in pharmaceutical sciences. Recombinant production of mecasermin in E. coli expression system. 2015 Sep: 9(6) 453-61 https://www.ncbi.nlm.nih.gov/pubmed/26339260

53 Wang Lili, Zhou Hu, Li Zhengjun, Lim Teck Kwang, Lim Xin Shan, Lin Qingsong Protein expression and purification. One-step extraction of functional recombinant aquaporin Z from inclusion bodies with optimal detergent. 2015 Sep: 115 146-52 https://www.ncbi.nlm.nih.gov/pubmed/26278820

54 Takayanagi Ayumi, Miyakawa Takuya, Asano Atsuko, Ohtsuka Jun, Tanokura Masaru, Arioka Manabu Protein expression and purification. Expression, purification, refolding, and enzymatic characterization of two secretory phospholipases A₂ from Neurospora crassa. 2015 Sep: 115 69-75 https://www.ncbi.nlm.nih.gov/pubmed/26272369

55 Herrmann Michaela, Danielczak Bartholomäus, Textor Martin, Klement Jessica, Keller Sandro European biophysics journal : EBJ. Modulating bilayer mechanical properties to promote the coupled folding and insertion of an integral membrane protein. 2015 Sep: 44(7) 503-12 https://www.ncbi.nlm.nih.gov/pubmed/26016666

56 Bajaj Priyanka, Tripathy Rajan K, Aggarwal Geetika, Pande Abhay H Protein expression and purification. Expression and purification of biologically active recombinant human paraoxonase 1 from inclusion bodies of Escherichia coli. 2015 Sep: 115 95-101 https://www.ncbi.nlm.nih.gov/pubmed/26003526

57 Fan Jiying, Huang Liying, Sun Jing, Qiu Yi, Zhou Jinsong, Shen Yaling Journal of biotechnology. Strategy for linker selection to enhance refolding and bioactivity of VAS-TRAIL fusion protein based on inclusion body conformation and activity. 2015 Aug: 209 16-22 https://www.ncbi.nlm.nih.gov/pubmed/26072465

58 Song Jinsue, Hong Hye Rim, Yamashita Eiki, Park Il Yeong, Lee Soo Jae Journal of biochemistry. Low pH-driven folding of WW45-SARAH domain leads to stabilization of the WW45-Mst2 complex. 2015 Aug: 158(3) 181-8 https://www.ncbi.nlm.nih.gov/pubmed/25814670

59 Zheng H, Mandal A, Shumilin I A, Chordia M D, Panneerdoss S, Herr J C, Minor W Andrology. Sperm Lysozyme-Like Protein 1 (SLLP1), an intra-acrosomal oolemmal-binding sperm protein, reveals filamentous organization in protein crystal form. 2015 Jul: 3(4) 756-71 https://www.ncbi.nlm.nih.gov/pubmed/26198801

60 Garnett James A, Diallo Mamou, Matthews Steve J Acta crystallographica. Purification, crystallization and preliminary X-ray diffraction analysis of the Escherichia coli common pilus chaperone EcpB. 2015 Jun: Section F, Structural biology communications. 71(Pt 6) 676-9 https://www.ncbi.nlm.nih.gov/pubmed/26057794

61 Bai Yuxiang, van der Kaaij Rachel Maria, Woortman Albert Jan Jacob, Jin Zhengyu, Dijkhuizen Lubbert BMC biotechnology. Characterization of the 4,6-α-glucanotransferase GTFB enzyme of Lactobacillus reuteri 121 isolated from inclusion bodies. 2015 Jun: 15 49 https://www.ncbi.nlm.nih.gov/pubmed/26050651

62 Zakharova G S, Poloznikov A A, Chubar T A, Gazaryan I G, Tishkov V I Protein expression and purification. High-yield reactivation of anionic tobacco peroxidase overexpressed in Escherichia coli. 2015 Jun: 113 85-93 https://www.ncbi.nlm.nih.gov/pubmed/25986322

63 Zhao Chunhui, Wang Dan, Feng Bin, Gou Meng, Liu Xin, Li Qingwei Comparative biochemistry and physiology. Identification and characterization of aldehyde dehydrogenase 9 from Lampetra japonica and its protective role against cytotoxicity. 2015 Jun: Part B, Biochemistry & molecular biology. 187 102-9 https://www.ncbi.nlm.nih.gov/pubmed/25981701

64 Odunuga Odutayo O, Adekoya Olayiwola A, Sylte Ingebrigt Protein expression and purification. High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification. 2015 Jun: 113 79-84 https://www.ncbi.nlm.nih.gov/pubmed/25979480

65 Shahana Pallichera Vijayan, Das Dipankar, Gontu Abhinay, Chandran Dev, Maithal Kapil Protein expression and purification. Efficient production of Tymovirus like particles displaying immunodominant epitopes of Japanese Encephalitis Virus envelope protein. 2015 Jun: 113 35-43 https://www.ncbi.nlm.nih.gov/pubmed/25959459

66 Köster Stefan, van Pee Katharina, Yildiz Özkan Methods in enzymology. Purification, Refolding, and Crystallization of the Outer Membrane Protein OmpG from Escherichia coli. 2015 May: 557 149-66 https://www.ncbi.nlm.nih.gov/pubmed/25950964

67 Tomisawa Satoshi, Sato Yuji, Kamiya Masakatsu, Kumaki Yasuhiro, Kikukawa Takashi, Kawano Keiichi, Demura Makoto, Nakamura Kiminori, Ayabe Tokiyoshi, Aizawa Tomoyasu Protein expression and purification. Efficient production of a correctly folded mouse α-defensin, cryptdin-4, by refolding during inclusion body solubilization. 2015 May: 112 21-8 https://www.ncbi.nlm.nih.gov/pubmed/25913370

68 Lindae Antje, Eberle Raphael J, Caruso Icaro P, Coronado Monika A, de Moraes Fabio R, Azevedo Vasco, Arni Raghuvir K Protein expression and purification. Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis. 2015 May: 112 15-20 https://www.ncbi.nlm.nih.gov/pubmed/25907380

69 Gao Jin, Wang Haixia Applied biochemistry and biotechnology. Prokaryotic Expression, Refolding and Purification of High-Purity Mouse Midkine in Escherichia coli. 2015 May: 176(2) 454-66 https://www.ncbi.nlm.nih.gov/pubmed/25813669

70 Qi Xingmei, Sun Yifan, Xiong Sidong Microbial cell factories. A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form. 2015 Apr: 14 24 https://www.ncbi.nlm.nih.gov/pubmed/25879903

71 Warepam Marina, Singh Laishram Rajendrakumar Archives of biochemistry and biophysics. Osmolyte mixtures have different effects than individual osmolytes on protein folding and functional activity. 2015 Apr: 573 77-83 https://www.ncbi.nlm.nih.gov/pubmed/25817170

72 Jo Geon-A, Lee Jong Min, No Gyuyou, Kang Dong Seop, Kim So-Hyun, Ahn Sun-Hee, Kong In-Soo Protein expression and purification. Isolation and characterization of a 17-kDa FKBP-type peptidyl-prolyl cis/trans isomerase from Vibrio anguillarum. 2015 Apr: 110 130-7 https://www.ncbi.nlm.nih.gov/pubmed/25747528

73 Chung Wen-Jen, Huang Chi-Lung, Gong Hong-Yi, Ou Tsung-Yin, Hsu Jue-Liang, Hu Shao-Yang Protein expression and purification. Recombinant production of biologically active giant grouper (Epinephelus lanceolatus) growth hormone from inclusion bodies of Escherichia coli by fed-batch culture. 2015 Apr: 110 79-88 https://www.ncbi.nlm.nih.gov/pubmed/25703054

74 Zaremba-Czogalla Magdalena, Stumpp Christian, Bonifacio Ezio, Paul Ralf Protein expression and purification. Comparison of the purification of biologically active IL-7 cytokine expressed in Escherichia coli and Pichia pastoris. 2015 Apr: 110 65-71 https://www.ncbi.nlm.nih.gov/pubmed/25703052

75 Kilian Tom-Marten, Beck-Sickinger Annette G Protein expression and purification. Recombinant expression and characterization of biologically active protein delta homolog 1. 2015 Apr: 110 72-8 https://www.ncbi.nlm.nih.gov/pubmed/25688756

76 Lee Young-Tae, Gibbons Garrett, Lee Shirley Y, Nikolovska-Coleska Zaneta, Dou Yali Protein expression and purification. One-pot refolding of core histones from bacterial inclusion bodies allows rapid reconstitution of histone octamer. 2015 Apr: 110 89-94 https://www.ncbi.nlm.nih.gov/pubmed/25687285

77 Zhang Xinke, Li Yan, Cheng Yanna, Tan Haining, Li Zhiwei, Qu Yi, Mu Guoying, Wang Fengshan Biochimica et biophysica acta. Tat PTD-endostatin: A novel anti-angiogenesis protein with ocular barrier permeability via eye-drops. 2015 Apr: 1850(6) 1140-9 https://www.ncbi.nlm.nih.gov/pubmed/25662072

78 Xu Jiang, Li Jia, Wu Xingan, Song Chen, Lin Ye, Shen Youxiao, Ye Wei, Sun Chenjing, Wang Xianni, Li ZhiChao, Liu Yi, Wei Longxiao, Li Zhuyi, Xu Zhikai Protein expression and purification. Expression and refolding of bioactive α-bungarotoxin V31 in E. coli. 2015 Apr: 110 30-6 https://www.ncbi.nlm.nih.gov/pubmed/25562180

79 Kumar Sandeep, Jain Kavish Kumar, Singh Anupam, Panda Amulya K, Kuhad Ramesh Chander Protein expression and purification. Characterization of recombinant pectate lyase refolded from inclusion bodies generated in E. coli BL21(DE3). 2015 Apr: 110 43-51 https://www.ncbi.nlm.nih.gov/pubmed/25497420

80 Yuan Jie, Zhou Huifang, Yang Yicong, Li Weimin, Wan Yi, Wang Lili Biomedical chromatography : BMC. Refolding and simultaneous purification of recombinant human proinsulin from inclusion bodies on protein-folding liquid-chromatography columns. 2015 Apr: 29(5) 777-82 https://www.ncbi.nlm.nih.gov/pubmed/25378200

81 Sharma Anand Kumar, Khandelwal Radhika, Sharma Yogendra, Rajanikanth Vangipurapu Protein expression and purification. Secretagogin, a hexa EF-hand calcium-binding protein: high level bacterial overexpression, one-step purification and properties. 2015 Mar: 109 113-9 https://www.ncbi.nlm.nih.gov/pubmed/25703053

82 Vemula Sandeep, Thunuguntla Rahul, Dedaniya Akshay, Kokkiligadda Sujana, Palle Chaitanya, Ronda Srinivasa Reddy Protein expression and purification. Improved Production and Characterization of Recombinant Human Granulocyte Colony Stimulating Factor from E. coli under Optimized Downstream Processes. 2015 Mar: 108 62-72 https://www.ncbi.nlm.nih.gov/pubmed/25659501

83 Sterckx Yann G-J, De Gieter Steven, Zorzini Valentina, Hadži San, Haesaerts Sarah, Loris Remy, Garcia-Pino Abel Protein expression and purification. An efficient method for the purification of proteins from four distinct toxin-antitoxin modules. 2015 Mar: 108 30-40 https://www.ncbi.nlm.nih.gov/pubmed/25582764

84 Kattner Christof, Pfennig Sabrina, Massari Paola, Tanabe Mikio Applied biochemistry and biotechnology. One-step purification and porin transport activity of the major outer membrane proteins P2 from Haemophilus influenzae, FomA from Fusobacterium nucleatum and PorB from Neisseria meningitidis. 2015 Mar: 175(6) 2907-15 https://www.ncbi.nlm.nih.gov/pubmed/25575589

85 Yamaguchi Hiroshi, Miyazaki Masaya Bioengineered. Microfluidic chips with multi-junctions: an advanced tool in recovering proteins from inclusion bodies. 2015 Mar: 6(1) 1-4 https://www.ncbi.nlm.nih.gov/pubmed/25531187

86 Dandage Rohan, Bandyopadhyay Anannya, Jayaraj Gopal Gunanathan, Saxena Kanika, Dalal Vijit, Das Aritri, Chakraborty Kausik ACS chemical biology. Classification of chemical chaperones based on their effect on protein folding landscapes. 2015 Mar: 10(3) 813-20 https://www.ncbi.nlm.nih.gov/pubmed/25493352

87 Ling Chunfang, Zhang Junyan, Chen Huifang, Zou Zehong, Lai He, Zhang Jianguo, Lin Deqiu, Tao Ailin Protein expression and purification. Expression and refolding of mite allergen pro-Der f1 from inclusion bodies in Escherichia coli. 2015 Mar: 109 93-8 https://www.ncbi.nlm.nih.gov/pubmed/25462803

88 Machuca Mayra A, Liu Yu C, Beckham Simone A, Roujeinikova Anna Acta crystallographica. Cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for multiple ligands (CcmL). 2015 Feb: Section F, Structural biology communications. 71(Pt 2) 211-6 https://www.ncbi.nlm.nih.gov/pubmed/25664798

89 Lukoyanova Natalya, Kondos Stephanie C, Farabella Irene, Law Ruby H P, Reboul Cyril F, Caradoc-Davies Tom T, Spicer Bradley A, Kleifeld Oded, Traore Daouda A K, Ekkel Susan M, Voskoboinik Ilia, Trapani Joseph A, Hatfaludi Tamas, Oliver Katherine, Hotze Eileen M, Tweten Rodney K, Whisstock James C, Topf Maya, Saibil Helen R, Dunstone Michelle A PLoS biology. Conformational changes during pore formation by the perforin-related protein pleurotolysin. 2015 Feb: 13(2) e1002049 https://www.ncbi.nlm.nih.gov/pubmed/25654333

90 Allahyari Mojgan, Mohabati Reyhaneh, Babaie Jalal, Amiri Samira, Siavashani Zahra Jafari, Zare Mehrak, Sadeghiani Ghazaleh, Golkar Majid Journal of immunological methods. Production of in-vitro refolded and highly antigenic SAG1 for development of a sensitive and specific Toxoplasma IgG ELISA. 2015 Feb: 416 157-66 https://www.ncbi.nlm.nih.gov/pubmed/25437069

91 Jamialahmadi Oveis, Fazeli Ahmad, Hashemi-Najafabadi Sameereh, Fazeli Mohammad Reza Biotechnology letters. A novel clot lysis assay for recombinant plasminogen activator. 2015 Feb: 37(3) 593-600 https://www.ncbi.nlm.nih.gov/pubmed/25351812

92 Machuca Mayra A, Liu Yu C, Roujeinikova Anna Acta crystallographica. Cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for aspartate A (CcaA). 2015 Jan: Section F, Structural biology communications. 71(Pt 1) 110-3 https://www.ncbi.nlm.nih.gov/pubmed/25615981

93 Gessmann Renate, Papadovasilaki Maria, Drougkas Evangelos, Petratos Kyriacos Acta crystallographica. Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing. 2015 Jan: Section F, Structural biology communications. 71(Pt 1) 19-23 https://www.ncbi.nlm.nih.gov/pubmed/25615962

94 Vemula Sandeep, Dedaniya Akshay, Thunuguntla Rahul, Mallu Maheswara Reddy, Parupudi Pavani, Ronda Srinivasa Reddy Journal of chromatography. Simplified in vitro refolding and purification of recombinant human granulocyte colony stimulating factor using protein folding cation exchange chromatography. 2015 Jan: A. 1379 74-82 https://www.ncbi.nlm.nih.gov/pubmed/25576039

95 Lyukmanova E N, Shulepko M A, Bychkov M L, Shenkarev Z O, Paramonov A S, Chugunov A O, Arseniev A S, Dolgikh D A, Kirpichnikov M P Acta naturae. Human SLURP-1 and SLURP-2 Proteins Acting on Nicotinic Acetylcholine Receptors Reduce Proliferation of Human Colorectal Adenocarcinoma HT-29 Cells. 2015 Jan: 6(4) 60-6 https://www.ncbi.nlm.nih.gov/pubmed/25558396

96 Kraševec Nada, Milunović Tatjana, Lasnik Marija Anžur, Lukančič Irena, Komel Radovan, Porekar Vladka Gaberc Acta chimica Slovenica. Human granulocyte colony stimulating factor (G-CSF) produced in the filamentous fungus Aspergillus niger. 2015 Jan: 61(4) 709-17 https://www.ncbi.nlm.nih.gov/pubmed/25551710

97 Chaturvedi Deepti, Mahalakshmi Radhakrishnan FEBS letters. Juxtamembrane tryptophans have distinct roles in defining the OmpX barrel-micelle boundary and facilitating protein-micelle association. 2015 Jan: 588(23) 4464-71 https://www.ncbi.nlm.nih.gov/pubmed/25448987

98 Kim Yana V, Gasparian Marine E, Bocharov Eduard V, Chertkova Rita V, Tkach Elena N, Dolgikh Dmitry A, Kirpichnikov Mikhail P Molecular biotechnology. New strategy for high-level expression and purification of biologically active monomeric TGF-β1/C77S in Escherichia coli. 2015 Jan: 57(2) 160-71 https://www.ncbi.nlm.nih.gov/pubmed/25370824

99 Wang Yan, Ren Wenxuan, Gao Dong, Wang Lili, Yang Ying, Bai Quan Biomedical chromatography : BMC. One-step refolding and purification of recombinant human tumor necrosis factor-α (rhTNF-α) using ion-exchange chromatography. 2015 Jan: 29(2) 305-11 https://www.ncbi.nlm.nih.gov/pubmed/24941919

100 Song Hyung-Nam, Jang Jun-Hyuck, Kim Young-Wan, Kim Dong-Hyung, Park Sung-Goo, Lee Myung Kyu, Paek Se-Hwan, Woo Eui-Jeon International journal of molecular sciences. Refolded scFv antibody fragment against myoglobin shows rapid reaction kinetics. 2014 Dec: 15(12) 23658-71 https://www.ncbi.nlm.nih.gov/pubmed/25530617

101 Lu Fang, Guo Gangxin, Li Qianqian, Feng Duo, Liu Yong, Huang Huoqing, Yang Peilong, Gao Wei, Yao Bin Acta crystallographica. Preparation, purification, crystallization and preliminary crystallographic analysis of dual-domain β-propeller phytase from Bacillus sp. HJB17. 2014 Dec: Section F, Structural biology communications. 70(Pt 12) 1671-4 https://www.ncbi.nlm.nih.gov/pubmed/25484224

102 Gull Nuzhat, Khan Javed Masood, Ishtikhar Mohd, Qadeer Atiyatul, Khan Rehan Ajmal, Gul Mudasir, Khan Rizwan Hasan Journal of colloid and interface science. Secondary structural changes in guanidinium hydrochloride denatured mammalian serum albumins and protective effect of small amounts of cationic gemini surfactant pentanediyl-α,ω-bis(cetyldimethylammonium bromide) and methyl-β-cyclodextrin: A spectroscopic study. 2014 Dec: 439 170-6 https://www.ncbi.nlm.nih.gov/pubmed/25463190

103 Boonyuen Usa, Promnares Kamoltip, Junkree Suwapat, Day Nichloas P J, Imwong Mallika Protein expression and purification. Efficient in vitro refolding and functional characterization of recombinant human liver carboxylesterase (CES1) expressed in E. coli. 2014 Dec: 107 68-75 https://www.ncbi.nlm.nih.gov/pubmed/25462813

104 Shi Tonglin, Zhang Lichao, Li Zhuoyu, Newton Ian P, Zhang Quanbin Protein expression and purification. Expression, purification and renaturation of truncated human integrin β1 from inclusion bodies of Escherichia coli. 2014 Dec: 107 13-9 https://www.ncbi.nlm.nih.gov/pubmed/25462808

105 Liu Yu Chih, Roujeinikova Anna Protein expression and purification. Expression, refolding, purification and crystallization of the sensory domain of the TlpC chemoreceptor from Helicobacter pylori for structural studies. 2014 Dec: 107 29-34 https://www.ncbi.nlm.nih.gov/pubmed/25462804

106 Zhao Dawei, Liu Yongdong, Zhang Guifeng, Zhang Chun, Li Xiunan, Wang Qingqing, Shi Hong, Su Zhiguo Biochimica et biophysica acta. Interaction of arginine with protein during refolding process probed by amide H/D exchange mass spectrometry and isothermal titration calorimetry. 2014 Dec: 1854(1) 39-45 https://www.ncbi.nlm.nih.gov/pubmed/25461798

107 Saremirad Pegah, Wood Jeffery A, Zhang Yan, Ray Ajay K Journal of chromatography. Oxidative protein refolding on size exclusion chromatography at high loading concentrations: fundamental studies and mathematical modeling. 2014 Dec: A. 1370 147-55 https://www.ncbi.nlm.nih.gov/pubmed/25454139

108 Lemke Laura Simoni, Chura-Chambi Rosa Maria, Rodrigues Daniella, Cussiol Jose Renato Rosa, Malavasi Natalia Vallejo, Alegria Thiago Geronimo Pires, Netto Luis Eduardo Soares, Morganti Ligia Protein expression and purification. Investigation on solubilization protocols in the refolding of the thioredoxin TsnC from Xylella fastidiosa by high hydrostatic pressure approach. 2014 Dec: 106 72-7 https://www.ncbi.nlm.nih.gov/pubmed/25448595

109 Pandey Veda P, Dwivedi Upendra N Gene. A ripening associated peroxidase from papaya having a role in defense and lignification: heterologous expression and in-silico and in-vitro experimental validation. 2014 Dec: 555(2) 438-47 https://www.ncbi.nlm.nih.gov/pubmed/25447898

110 Xiao Huogen, Bryksa Brian C, Bhaumik Prasenjit, Gustchina Alla, Kiso Yoshiaki, Yao Shao Q, Wlodawer Alexander, Yada Rickey Y Molecular and biochemical parasitology. The zymogen of plasmepsin V from Plasmodium falciparum is enzymatically active. 2014 Dec: 197(1-2) 56-63 https://www.ncbi.nlm.nih.gov/pubmed/25447707

111 Brecher Matthew B, Li Zhong, Zhang Jing, Chen Hui, Lin Qishan, Liu Binbin, Li Hongmin Protein science : a publication of the Protein Society. Refolding of a fully functional flavivirus methyltransferase revealed that S-adenosyl methionine but not S-adenosyl homocysteine is copurified with flavivirus methyltransferase. 2014 Dec: 24(1) 117-28 https://www.ncbi.nlm.nih.gov/pubmed/25352331

112 Singh Krishna Kumar, Jain Ruchi, Ramanan Harini, Saini Deepak Kumar Molecular biotechnology. Matrix-assisted refolding, purification and activity assessment using a 'form invariant' assay for matrix metalloproteinase 2 (MMP2). 2014 Nov: 56(12) 1121-32 https://www.ncbi.nlm.nih.gov/pubmed/25119648

113 Warepam Marina, Sharma Gurumayum Suraj, Dar Tanveer Ali, Khan Md Khurshid Alam, Singh Laishram Rajendrakumar PloS one. Structural characteristic of the initial unfolded state on refolding determines catalytic efficiency of the folded protein in presence of osmolytes. 2014 Oct: 9(10) e109408 https://www.ncbi.nlm.nih.gov/pubmed/25313668

114 Upadhyay Arun K, Singh Anupam, Mukherjee K J, Panda Amulya K Frontiers in microbiology. Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein. 2014 Oct: 5 486 https://www.ncbi.nlm.nih.gov/pubmed/25309524

115 Carlomagno Mariana, Lassabe Gabriel, Rossotti Martín, González-Techera Andrés, Vanrell Lucía, González-Sapienza Gualberto Analytical chemistry. Recombinant streptavidin nanopeptamer anti-immunocomplex assay for noncompetitive detection of small analytes. 2014 Oct: 86(20) 10467-73 https://www.ncbi.nlm.nih.gov/pubmed/25257512

116 Faulin Tanize do Espirito Santo, Guilherme Daniel Ferreira, Silva Aldacilene Souza, Abdalla Dulcineia Saes Parra, Hering Vitor Renaux, Politi Mario José, Maranhão Andrea Queiroz Biotechnology progress. GFP-SCFV: expression and possible applications as a tool for experimental investigations of atherosclerosis. 2014 Oct: 30(5) 1206-13 https://www.ncbi.nlm.nih.gov/pubmed/24911875

117 Elter Shantha, Raschle Thomas, Arens Sabine, Viegas Aldino, Gelev Vladimir, Etzkorn Manuel, Wagner Gerhard The Journal of membrane biology. The use of amphipols for NMR structural characterization of 7-TM proteins. 2014 Oct: 247(9-10) 957-64 https://www.ncbi.nlm.nih.gov/pubmed/24858950

118 Ahmed Nadeem, Zafar Ahmad Usman, Khan Mohsin Ahmad, Tahir Saad, Khan Muhammad Islam, Bashir Hamid, Khan Faidad, Sarwar Samreen, Ilyas Sadaf, Husnain Tayyab Biotechnology and applied biochemistry. Matrix-assisted refolding and purification of placenta-derived recombinant human interleukin-6 produced in Escherichia coli. 2014 Oct: 61(5) 541-8 https://www.ncbi.nlm.nih.gov/pubmed/24397399

119 Gupta Ankit, Zadafiya Punit, Mahalakshmi Radhakrishnan Scientific reports. Differential contribution of tryptophans to the folding and stability of the attachment invasion locus transmembrane β-barrel from Yersinia pestis. 2014 Sep: 4 6508 https://www.ncbi.nlm.nih.gov/pubmed/25266561

120 Checchetto Vanessa, Reina Simona, Magrì Andrea, Szabo Ildikò, De Pinto Vito Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. Recombinant human voltage dependent anion selective channel isoform 3 (hVDAC3) forms pores with a very small conductance. 2014 Sep: 34(3) 842-53 https://www.ncbi.nlm.nih.gov/pubmed/25171321

121 Dolgikh V V, Senderskiy I V, Tetz G V, Tetz V V Acta naturae. Optimization of the Protocol for the Isolation and Refolding of the Extracellular Domain of HER2 Expressed in Escherichia coli. 2014 Aug: 6(2) 106-9 https://www.ncbi.nlm.nih.gov/pubmed/25093117

122 Saremirad Pegah, Wood Jeffery A, Zhang Yan, Ray Ajay K Journal of chromatography. Multi-variable operational characteristic studies of on-column oxidative protein refolding at high loading concentrations. 2014 Aug: A. 1359 70-5 https://www.ncbi.nlm.nih.gov/pubmed/25092593

123 Sammar Marei, Nisamblatt Shahar, Gonen Ron, Huppertz Berthold, Gizurarson Sveinbjorn, Osol George, Meiri Hamutal PloS one. The role of the carbohydrate recognition domain of placental protein 13 (PP13) in pregnancy evaluated with recombinant PP13 and the DelT221 PP13 variant. 2014 Aug: 9(7) e102832 https://www.ncbi.nlm.nih.gov/pubmed/25079598

124 Tischer Alexander, Lilie Hauke, Auton Matthew, Lange Christian Biopolymers. Oxidative refolding of rPA in l-ArgHCl and in ionic liquids: A correlation between hydrophobicity, salt effects, and refolding yield. 2014 Aug: 101(11) 1129-40 https://www.ncbi.nlm.nih.gov/pubmed/24931846

125 Verlotta Angelo, Trono Daniela Protein expression and purification. Expression, purification and refolding of active durum wheat (Triticum durum Desf.) secretory phospholipase A2 from inclusion bodies of Escherichia coli. 2014 Aug: 101 28-36 https://www.ncbi.nlm.nih.gov/pubmed/24925645

126 Ma Y, Jin X-B, Chu F-J, Bao D-Mei, Zhu J-Y Applied microbiology and biotechnology. Expression of liver-targeting peptide modified recombinant human endostatin and preliminary study of its biological activities. 2014 Aug: 98(18) 7923-33 https://www.ncbi.nlm.nih.gov/pubmed/24908076

127 Ferraris Davide M, Spallek Ralf, Oehlmann Wulf, Singh Mahavir, Rizzi Menico Proteins. Crystal structure of the Mycobacterium tuberculosis phosphate binding protein PstS3. 2014 Aug: 82(9) 2268-74 https://www.ncbi.nlm.nih.gov/pubmed/24615888

128 Künze Georg, Theisgen Stephan, Huster Daniel Biomolecular NMR assignments. Backbone ¹H, ¹⁵N, and ¹³C and side chain ¹³Cβ NMR chemical shift assignment of murine interleukin-10. 2014 Aug: 8(2) 375-8 https://www.ncbi.nlm.nih.gov/pubmed/23982919

129 Whiteman Pat, Redfield Christina, Handford Penny A Methods in molecular biology (Clifton, N. Bacterial expression and in vitro refolding of limited fragments of the Notch receptor and its ligands. 2014 Jul:J.). 1187 193-208 https://www.ncbi.nlm.nih.gov/pubmed/25053491

130 Satoh Yuki, Miki Shinsuke, Ose Toyoyuki, Oikawa Azusa, Maenaka Katsumi, Terauchi Ryouhei, Asano Kozo, Sone Teruo Bioscience, biotechnology, and biochemistry. Heterologous production, purification, and immunodetection of Magnaporthe oryzae avirulence protein AVR-Pia. 2014 Jul: 78(4) 680-6 https://www.ncbi.nlm.nih.gov/pubmed/25036967

131 Tseng Chih-Wen, Liao Chien-Yi, Sun Yuanxia, Peng Chi-Chung, Tzen Jason T C, Guo Rey-Ting, Liu Je-Ruei Journal of agricultural and food chemistry. Immobilization of Clostridium cellulolyticum D-psicose 3-epimerase on artificial oil bodies. 2014 Jul: 62(28) 6771-6 https://www.ncbi.nlm.nih.gov/pubmed/24980476

132 Moricoli Diego, Muller William Anthony, Carbonella Damiano Cosimo, Balducci Maria Cristina, Dominici Sabrina, Watson Richard, Fiori Valentina, Weber Evan, Cianfriglia Maurizio, Scotlandi Katia, Magnani Mauro Journal of immunological methods. Blocking monocyte transmigration in in vitro system by a human antibody scFv anti-CD99. Efficient large scale purification from periplasmic inclusion bodies in E. coli expression system. 2014 Jul: 408 35-45 https://www.ncbi.nlm.nih.gov/pubmed/24798881

133 Jeong Seon-Ju, Cho Kye Man, Lee Chang Kwon, Kim Gyoung Min, Shin Jung-Hye, Kim Jong Sang, Kim Jeong Hwan Journal of microbiology and biotechnology. Overexpression of aprE2, a fibrinolytic enzyme gene from Bacillus subtilis CH3-5, in Escherichia coli and the properties of AprE2. 2014 Jul: 24(7) 969-78 https://www.ncbi.nlm.nih.gov/pubmed/24743573

134 Wang Fei, Guo Jinjin, Bai Quan, Wang Lili Biotechnology progress. Refolding and purification of recombinant human (Pro)renin receptor from Escherichia coli by ion exchange chromatography. 2014 Jul: 30(4) 864-71 https://www.ncbi.nlm.nih.gov/pubmed/24723509

135 Sun Chenjing, Zhang Hongliang, Xu Jiang, Gao Jie, Qi Xiaokun, Li Zhuyi Archives of medical science : AMS. Improved methodology to obtain large quantities of correctly folded recombinant N-terminal extracellular domain of the human muscle acetylcholine receptor for inducing experimental autoimmune myasthenia gravis in rats. 2014 Jun: 10(2) 389-95 https://www.ncbi.nlm.nih.gov/pubmed/24904677

136 Raghunathan Govindan, Munussami Ganapathiraman, Moon Hyojin, Paik Hyun-jong, An Seong Soo A, Kim Yong-Sung, Kang Sebyung, Lee Sun-Gu Microbial cell factories. A variant of green fluorescent protein exclusively deposited to active intracellular inclusion bodies. 2014 Jun: 13 68 https://www.ncbi.nlm.nih.gov/pubmed/24885571

137 Nguyen Sy Le Thanh, Quyen Dinh Thi, Vu Hong Diep BioMed research international. Highly effective renaturation of a streptokinase from Streptococcus pyogenes DT7 as inclusion bodies overexpressed in Escherichia coli. 2014 Jun: 2014 324705 https://www.ncbi.nlm.nih.gov/pubmed/24883307

138 Barden Stephan, Schomburg Benjamin, Conradi Jens, Backert Steffen, Sewald Norbert, Niemann Hartmut H Acta crystallographica. Structure of a three-dimensional domain-swapped dimer of the Helicobacter pylori type IV secretion system pilus protein CagL. 2014 May: Section D, Biological crystallography. 70(Pt 5) 1391-400 https://www.ncbi.nlm.nih.gov/pubmed/24816107

139 Niu Jianlou, Zhu Yanlin, Song Lintao, Xie Yaoyao, Zhang Yi, Wang Huiyan, Li Xiaokun, Liu Bailing, Cai Lu, Huang Zhifeng Bioconjugate chemistry. One-step production of bioactive proteins through simultaneous PEGylation and refolding. 2014 May: 25(1) 63-71 https://www.ncbi.nlm.nih.gov/pubmed/24341776

140 Cesarini Silvia, Infanzón Belén, Pastor F I Javier, Diaz Pilar BMC biotechnology. Fast and economic immobilization methods described for non-commercial Pseudomonas lipases. 2014 Apr: 14 27 https://www.ncbi.nlm.nih.gov/pubmed/24755191

141 Rothan Hussin A, Bahrani Hirbod, Mohamed Zulqarnain, Abd Rahman Noorsaadah, Yusof Rohana PloS one. Fusion of protegrin-1 and plectasin to MAP30 shows significant inhibition activity against dengue virus replication. 2014 Apr: 9(4) e94561 https://www.ncbi.nlm.nih.gov/pubmed/24722532

142 Mathew Manjumol, Sreedhanya S, Manoj P, Aravindakumar C T, Aravind Usha K The journal of physical chemistry. Exploring the interaction of bisphenol-S with serum albumins: a better or worse alternative for bisphenol a? 2014 Apr: B. 118(14) 3832-43 https://www.ncbi.nlm.nih.gov/pubmed/24635450

143 Wang Ci, Zhan Chun-Lan, Cai Qiu-Feng, Du Cui-Hong, Liu Guang-Ming, Su Wen-Jin, Cao Min-Jie Journal of biotechnology. Expression and characterization of common carp (Cyprinus carpio) matrix metalloproteinase-2 and its activity against type I collagen. 2014 Apr: 177 45-52 https://www.ncbi.nlm.nih.gov/pubmed/24613299

144 Lim Sungsoo, Chundawat Shishir P S, Fox Brian G Protein expression and purification. Expression, purification and characterization of a functional carbohydrate-binding module from Streptomyces sp. SirexAA-E. 2014 Apr: 98 1-9 https://www.ncbi.nlm.nih.gov/pubmed/24607362

145 Suhre Michael H, Scheibel Thomas Journal of structural biology. Structural diversity of a collagen-binding matrix protein from the byssus of blue mussels upon refolding. 2014 Apr: 186(1) 75-85 https://www.ncbi.nlm.nih.gov/pubmed/24583234

146 Wang Xiao-Min, Guo Zhan-Yun Biomedical reports. Recombinant expression, different downstream processing of the disulfide-rich anti-tumor peptide Ranpirnase and its effect on the growth of human glioma cell line SHG-44. 2014 Mar: 1(5) 747-750 https://www.ncbi.nlm.nih.gov/pubmed/24649022

147 Yuasa Noriyuki, Koyama Tsubasa, Fujita-Yamaguchi Yoko Bioscience trends. Purification and refolding of anti-T-antigen single chain antibodies (scFvs) expressed in Escherichia coli as inclusion bodies. 2014 Mar: 8(1) 24-31 https://www.ncbi.nlm.nih.gov/pubmed/24647109

148 Ni Dongchun, Yang Kun, Huang Yihua Acta crystallographica. Refolding, crystallization and preliminary X-ray crystallographic studies of the β-barrel domain of BamA, a membrane protein essential for outer membrane protein biogenesis. 2014 Mar: Section F, Structural biology communications. 70(Pt 3) 362-5 https://www.ncbi.nlm.nih.gov/pubmed/24598928

149 dos Santos Marlus Alves, Teixeira Francesco Brugnera, Moreira Heline Hellen Teixeira, Rodrigues Adele Aud, Machado Fabrício Castro, Clemente Tatiana Mordente, Brigido Paula Cristina, Silva Rebecca Tavares e, Purcino Cecílio, Gomes Rafael Gonçalves Barbosa, Bahia Diana, Mortara Renato Arruda, Munte Claudia Elisabeth, Horjales Eduardo, da Silva Claudio Vieira Scientific reports. A successful strategy for the recovering of active P21, an insoluble recombinant protein of Trypanosoma cruzi. 2014 Mar: 4 4259 https://www.ncbi.nlm.nih.gov/pubmed/24590372

150 Lolupiman Sirisak, Siripurkpong Pilaiwan, Yuvaniyama Jirundon PloS one. Disulfide linkages in Plasmodium falciparum plasmepsin-i are essential elements for its processing activity and multi-milligram recombinant production yield. 2014 Mar: 9(2) e89424 https://www.ncbi.nlm.nih.gov/pubmed/24586769

151 Naz Farha, Asad Mohd, Malhotra Pawan, Islam Asimul, Ahmad Faizan, Hassan Md Imtaiyaz Applied biochemistry and biotechnology. Cloning, expression, purification and refolding of microtubule affinity-regulating kinase 4 expressed in Escherichia coli. 2014 Mar: 172(6) 2838-48 https://www.ncbi.nlm.nih.gov/pubmed/24446173

152 Kumar Rajesh, Angov Evelina, Kumar Nirbhay Infection and immunity. Potent malaria transmission-blocking antibody responses elicited by Plasmodium falciparum Pfs25 expressed in Escherichia coli after successful protein refolding. 2014 Mar: 82(4) 1453-9 https://www.ncbi.nlm.nih.gov/pubmed/24421036

153 Jia Dongxu, Yang Yu, Peng Zhengcong, Zhang Dongxu, Li Jianghua, Liu Long, Du Guocheng, Chen Jian Applied biochemistry and biotechnology. High efficiency preparation and characterization of intact poly(vinyl alcohol) dehydrogenase from Sphingopyxis sp.113P3 in Escherichia coli by inclusion bodies renaturation. 2014 Mar: 172(5) 2540-51 https://www.ncbi.nlm.nih.gov/pubmed/24402569

154 Kim Chang-Kyu, Choi Jun-Ha, Lee Seung-Bae, Lee Sang-Mahn, Oh Jae-Wook Applied biochemistry and biotechnology. Expression and purification of recombinant human granulocyte colony-stimulating factor in fed-batch culture of Escherichia coli. 2014 Mar: 172(5) 2425-35 https://www.ncbi.nlm.nih.gov/pubmed/24390866

155 Iram Nida, Shah Muhammad Salahuddin, Ismat Fouzia, Habib Mudasser, Iqbal Mazhar, Hasnain S Samar, Rahman Moazur Applied microbiology and biotechnology. Heterologous expression, characterization and evaluation of the matrix protein from Newcastle disease virus as a target for antiviral therapies. 2014 Mar: 98(4) 1691-701 https://www.ncbi.nlm.nih.gov/pubmed/23797330

156 Kuo Mario Meng-Chiang, Nguyen Phuong Hong, Jeon Yun-Hui, Kim Subin, Yoon So-Mi, Choe Senyon Microbial cell factories. MB109 as bioactive human bone morphogenetic protein-9 refolded and purified from E. coli inclusion bodies. 2014 Feb: 13(1) 29 https://www.ncbi.nlm.nih.gov/pubmed/24559319

157 Picciocchi Antoine, Siaučiūnaiteė-Gaubard Lina, Petit-Hartlein Isabelle, Sadir Rabia, Revilloud Jean, Caro Lydia, Vivaudou Michel, Fieschi Franck, Moreau Christophe, Vivès Corinne PloS one. C-terminal engineering of CXCL12 and CCL5 chemokines: functional characterization by electrophysiological recordings. 2014 Feb: 9(1) e87394 https://www.ncbi.nlm.nih.gov/pubmed/24498095

158 Maurya Svetlana Rajkumar, Mahalakshmi Radhakrishnan PloS one. Influence of protein-micelle ratios and cysteine residues on the kinetic stability and unfolding rates of human mitochondrial VDAC-2. 2014 Feb: 9(1) e87701 https://www.ncbi.nlm.nih.gov/pubmed/24494036

159 Rodrigues D, Farinha-Arcieri L E, Ventura A M, Chura-Chambi R M, Malavasi N V, Lemke L S, Guimarães J S, Ho P L, Morganti L Journal of biotechnology. Effect of pressure on refolding of recombinant pentameric cholera toxin B. 2014 Feb: 173 98-105 https://www.ncbi.nlm.nih.gov/pubmed/24445168

160 Yuan Ruosen, Chen Xiaoxu, Chen Yan, Gu Tiejun, Xi Hualong, Duan Ye, Sun Bo, Yu Xianghui, Jiang Chunlai, Liu Xintao, Wu Chunlai, Kong Wei, Wu Yongge Applied microbiology and biotechnology. Preparation and diagnostic use of a novel recombinant single-chain antibody against rabies virus glycoprotein. 2014 Feb: 98(4) 1547-55 https://www.ncbi.nlm.nih.gov/pubmed/24241896

161 Barroso L, Abhyankar M, Noor Z, Read K, Pedersen K, White R, Fox C, Petri W A, Lyerly D Vaccine. Expression, purification, and evaluation of recombinant LecA as a candidate for an amebic colitis vaccine. 2014 Feb: 32(10) 1218-24 https://www.ncbi.nlm.nih.gov/pubmed/23827311

162 Lu Yuan, Welsh John P, Swartz James R Proceedings of the National Academy of Sciences of the United States of America. Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines. 2014 Jan: 111(1) 125-30 https://www.ncbi.nlm.nih.gov/pubmed/24344259

163 Amirzada Muhammad Imran, Yu Minglei, Gong Xiaohai, Chen Yun, Zhu Ruiyu, Lei Jianyong, Jin Jian Journal of industrial microbiology & biotechnology. Cost-effective production of recombinant human interleukin 24 by lactose induction and a two-step denaturing and one-step refolding method. 2014 Jan: 41(1) 135-42 https://www.ncbi.nlm.nih.gov/pubmed/24174213

164 Blancas-Mejía Luis M, Tischer Alexander, Thompson James R, Tai Jonathan, Wang Lin, Auton Matthew, Ramirez-Alvarado Marina Journal of molecular biology. Kinetic control in protein folding for light chain amyloidosis and the differential effects of somatic mutations. 2014 Jan: 426(2) 347-61 https://www.ncbi.nlm.nih.gov/pubmed/24157440

165 Parui Partha Pratim, Deshpande Megha Subhash, Nagao Satoshi, Kamikubo Hironari, Komori Hirofumi, Higuchi Yoshiki, Kataoka Mikio, Hirota Shun Biochemistry. Formation of oligomeric cytochrome c during folding by intermolecular hydrophobic interaction between N- and C-terminal α-helices. 2013 Dec: 52(48) 8732-44 https://www.ncbi.nlm.nih.gov/pubmed/24206001

166 Chura-Chambi Rosa M, Nakajima Erika, de Carvalho Roberta R, Miyasato Patricia A, Oliveira Sergio C, Morganti Ligia, Martins Elizabeth A L Journal of biotechnology. Refolding of the recombinant protein Sm29, a step toward the production of the vaccine candidate against schistosomiasis. 2013 Dec: 168(4) 511-9 https://www.ncbi.nlm.nih.gov/pubmed/24084635

167 Tomar Sushil Kumar, Knauer Stefan H, Nandymazumdar Monali, Rösch Paul, Artsimovitch Irina Nucleic acids research. Interdomain contacts control folding of transcription factor RfaH. 2013 Dec: 41(22) 10077-85 https://www.ncbi.nlm.nih.gov/pubmed/23990324

168 Han Long, Cao Min-Jie, Shi Chao-lan, Wei Xiao-Nan, Li Huan, Du Cui-Hong Comparative biochemistry and physiology. cDNA cloning of glucose-6-phosphate isomerase from crucian carp (Carassius carassius) and expression of the active region as myofibril-bound serine proteinase inhibitor in Escherichia coli. 2013 Dec: Part B, Biochemistry & molecular biology. 168 86-93 https://www.ncbi.nlm.nih.gov/pubmed/23774640

169 Wellhoefer Martin, Sprinzl Wolfgang, Hahn Rainer, Jungbauer Alois Journal of chromatography. Continuous processing of recombinant proteins: Integration of inclusion body solubilization and refolding using simulated moving bed size exclusion chromatography with buffer recycling. 2013 Nov: A. 1319 107-17 https://www.ncbi.nlm.nih.gov/pubmed/24169042

170 Chaturvedi Deepti, Mahalakshmi Radhakrishnan PloS one. Methionine mutations of outer membrane protein X influence structural stability and beta-barrel unfolding. 2013 Nov: 8(11) e79351 https://www.ncbi.nlm.nih.gov/pubmed/24265768

171 Junaid M, Angsuthanasombat C, Wikberg J E S, Ali N, Katzenmeier G Biochemistry. Modulation of enzymatic activity of dengue virus nonstructural protein NS3 nucleoside triphosphatase/helicase by poly(U). 2013 Nov: Biokhimii︠a︡. 78(8) 925-32 https://www.ncbi.nlm.nih.gov/pubmed/24228882

172 Junaid M, Angsuthanasombat C, Wikberg J E S, Ali N, Katzenmeier G Biochemistry. A straightforward experimental approach to expression, purification, refolding, and enzymatic analysis of recombinant dengue virus NS2B(H)-NS3pro protease. 2013 Nov: Biokhimii︠a︡. 78(8) 920-4 https://www.ncbi.nlm.nih.gov/pubmed/24228881

173 Kim Chang Kyu, Lee Chi Ho, Lee Seung-Bae, Oh Jae-Wook PloS one. Simplified large-scale refolding, purification, and characterization of recombinant human granulocyte-colony stimulating factor in Escherichia coli. 2013 Nov: 8(11) e80109 https://www.ncbi.nlm.nih.gov/pubmed/24224041

174 Yuzaki Kota, Sanda Yudai, You Dong-Ju, Uehara Ryo, Koga Yuichi, Kanaya Shigenori Protein science : a publication of the Protein Society. Increase in activation rate of Pro-Tk-subtilisin by a single nonpolar-to-polar amino acid substitution at the hydrophobic core of the propeptide domain. 2013 Nov: 22(12) 1711-21 https://www.ncbi.nlm.nih.gov/pubmed/24115021

175 Ojopagogo Yetunde Adedolapo, Adewale Isaac Olusanjo, Afolayan Adeyinka Fish physiology and biochemistry. Preliminary studies on the renaturation of denatured catfish (Clarias gariepinus) glutathione transferase. 2013 Nov: 39(6) 1657-63 https://www.ncbi.nlm.nih.gov/pubmed/23748965

176 Zhou Yaqiong, Zhang Juan, Jin Haizhen, Chen Zhiguo, Wu Qinhang, Li Weiguang, Yue Ming, Luo Chen, Wang Min Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. Prokaryotic expression and refolding of EGFR extracellular domain and generation of phage display human scFv against EGFR. 2013 Nov: 67(8) 737-43 https://www.ncbi.nlm.nih.gov/pubmed/23639232

177 Wang Xiao-Min, Luo Xiao, Guo Zhan-Yun Experimental and therapeutic medicine. Recombinant expression and downstream processing of the disulfide-rich tumor-targeting peptide chlorotoxin. 2013 Oct: 6(4) 1049-1053 https://www.ncbi.nlm.nih.gov/pubmed/24137314

178 Gai Zuoqi, Nakamura Akiyoshi, Tanaka Yoshikazu, Hirano Nagisa, Tanaka Isao, Yao Min Journal of synchrotron radiation. Crystal structure analysis, overexpression and refolding behaviour of a DING protein with single mutation. 2013 Oct: 20(Pt 6) 854-8 https://www.ncbi.nlm.nih.gov/pubmed/24121327

179 Rognoni Paola, Lavatelli Francesca, Casarini Simona, Palladini Giovanni, Verga Laura, Pedrazzoli Paolo, Valentini Giovanna, Merlini Giampaolo, Perfetti Vittorio PloS one. A strategy for synthesis of pathogenic human immunoglobulin free light chains in E. coli. 2013 Oct: 8(9) e76022 https://www.ncbi.nlm.nih.gov/pubmed/24086679

180 Bae Sang-Woo, Ahn Kihun, Koo Yoon-Mo, Ha Sung Ho Applied biochemistry and biotechnology. Refolding of laccase in dilution additive mode with copper-based ionic liquid. 2013 Oct: 171(5) 1289-98 https://www.ncbi.nlm.nih.gov/pubmed/23975279

181 Venables Luanne, Govender Sharlene, Oosthuizen Vaughan Protein expression and purification. Heterologous expression and purification of biologically active domains 3 and 4 of human polymeric immunoglobulin receptor and its interaction with choline binding protein A of Streptococcus pneumoniae. 2013 Sep: 91(2) 207-14 https://www.ncbi.nlm.nih.gov/pubmed/23973337

182 Su Qiudong, Yi Yao, Guo Minzhuo, Qiu Feng, Jia Zhiyuan, Lu Xuexin, Meng Qingling, Bi Shengli Biochemical and biophysical research communications. Construction and immunological evaluation of truncated hepatitis B core particles carrying HBsAg amino acids 119-152 in the major immunodominant region (MIR). 2013 Sep: 439(1) 84-9 https://www.ncbi.nlm.nih.gov/pubmed/23969156

183 Mizukami Takuya, Xu Ming, Cheng Hong, Roder Heinrich, Maki Kosuke Protein science : a publication of the Protein Society. Nonuniform chain collapse during early stages of staphylococcal nuclease folding detected by fluorescence resonance energy transfer and ultrarapid mixing methods. 2013 Sep: 22(10) 1336-48 https://www.ncbi.nlm.nih.gov/pubmed/23904284

184 Aslam Farheen, Gardner Qurra-tul Ann Afza, Zain Hina, Nadeem Muhammad Shahid, Ali Muhammad, Rashid Naeem, Akhtar Muhammad Biochimica et biophysica acta. Studies on the expression and processing of human proinsulin derivatives encoded by different DNA constructs. 2013 Sep: 1834(10) 2116-23 https://www.ncbi.nlm.nih.gov/pubmed/23872484

185 Hacke Mandy, Gruber Tobias, Schulenburg Cindy, Balbach Jochen, Arnold Ulrich The FEBS journal. Consequences of proline-to-alanine substitutions for the stability and refolding of onconase. 2013 Sep: 280(18) 4454-62 https://www.ncbi.nlm.nih.gov/pubmed/23796075

186 Timmes Andrew G, Moore Roger A, Fischer Elizabeth R, Priola Suzette A PloS one. Recombinant prion protein refolded with lipid and RNA has the biochemical hallmarks of a prion but lacks in vivo infectivity. 2013 Aug: 8(7) e71081 https://www.ncbi.nlm.nih.gov/pubmed/23936256

187 Khodabakhsh Fatemeh, Dehghani Zohreh, Zia Mohammad Farid, Rabbani Mohammad, Sadeghi Hamid Mir Mohammad Avicenna journal of medical biotechnology. Cloning and Expression of Functional Reteplase in Escherichia coli TOP10. 2013 Aug: 5(3) 168-75 https://www.ncbi.nlm.nih.gov/pubmed/23919120

188 Novototskaya-Vlasova Ksenia, Petrovskaya Lada, Kryukova Elena, Rivkina Elizaveta, Dolgikh Dmitry, Kirpichnikov Mikhail Protein expression and purification. Expression and chaperone-assisted refolding of a new cold-active lipase from Psychrobacter cryohalolentis K5(T). 2013 Aug: 91(1) 96-103 https://www.ncbi.nlm.nih.gov/pubmed/23891837

189 Wellhoefer Martin, Sprinzl Wolfgang, Hahn Rainer, Jungbauer Alois Journal of chromatography. Autoprotease N(pro): analysis of self-cleaving fusion protein. 2013 Aug: A. 1304 92-100 https://www.ncbi.nlm.nih.gov/pubmed/23871287

190 Eleniste Pierre P, Hofstetter Heike, Hofstetter Oliver Protein expression and purification. Expression and characterization of an enantioselective antigen-binding fragment directed against α-amino acids. 2013 Aug: 91(1) 20-9 https://www.ncbi.nlm.nih.gov/pubmed/23827208

191 Sánchez-Arreola Pamela B, López-Uriarte Salvador, Marichal-Gallardo Pável A, González-Vázquez Juan C, Pérez-Chavarría Roberto, Soto-Vázquez Pedro, López-Pacheco Felipe, Ramírez-Medrano Alicia, Rocha-Pizaña María R, Alvarez Mario M Biotechnology progress. A baseline process for the production, recovery, and purification of bacterial influenza vaccine candidates. 2013 Aug: 29(4) 896-908 https://www.ncbi.nlm.nih.gov/pubmed/23749362

192 Steffler Fabian, Sieber Volker PloS one. Refolding of a thermostable glyceraldehyde dehydrogenase for application in synthetic cascade biomanufacturing. 2013 Jul: 8(7) e70592 https://www.ncbi.nlm.nih.gov/pubmed/23894676

193 Rane Anuja M, Jonnalagadda Sriramakamal, Li Zhiyu Protein expression and purification. On-column refolding of bone morphogenetic protein-2 using cation exchange resin. 2013 Jul: 90(2) 135-40 https://www.ncbi.nlm.nih.gov/pubmed/23748143

194 Ursel Christian, Fandrich Uwe, Hoffmann Anita, Sieg Torsten, Ihling Christian, Stubbs Milton T Biological chemistry. In vitro maturation of Drosophila melanogaster Spätzle protein with refolded Easter reveals a novel cleavage site within the prodomain. 2013 Jul: 394(8) 1069-75 https://www.ncbi.nlm.nih.gov/pubmed/23729565

195 Kawano Susumu, Iyaguchi Daisuke, Okada Chiaki, Sasaki Yusuke, Toyota Eiko The protein journal. Expression, purification, and refolding of active recombinant human E-selectin lectin and EGF domains in Escherichia coli. 2013 Jul: 32(5) 386-91 https://www.ncbi.nlm.nih.gov/pubmed/23716035

196 Wujak Magdalena, Banach Mariusz, Porowińska Dorota, Piskulak Katarzyna, Komoszyński Michał Phytochemistry. Isolation and bioinformatic analysis of seven genes encoding potato apyrase. Bacterial overexpresssion, refolding and initial kinetic studies on some recombinant potato apyrases. 2013 Jul: 93 8-17 https://www.ncbi.nlm.nih.gov/pubmed/23663929

197 Yang Chun-Yan, Li Ming, Dong Xiao-Yan, Sun Yan Journal of chromatography. A double-modification strategy for enhancing charge density of mono-sized beads for facilitated refolding of like-charged protein. 2013 Jun: A. 1299 85-93 https://www.ncbi.nlm.nih.gov/pubmed/23786785

198 Patke Sanket, Srinivasan Saipraveen, Maheshwari Ronak, Srivastava Sunit K, Aguilera J Javier, Colón Wilfredo, Kane Ravi S PloS one. Characterization of the oligomerization and aggregation of human Serum Amyloid A. 2013 Jun: 8(6) e64974 https://www.ncbi.nlm.nih.gov/pubmed/23750222

199 Fan Jingjing, Liu Yi, Xie Xuping, Zhang Bo, Yuan Zhiming Virologica Sinica. Inhibition of Japanese encephalitis virus infection by flavivirus recombinant E protein domain III. 2013 Jun: 28(3) 152-60 https://www.ncbi.nlm.nih.gov/pubmed/23709058

200 Oldefest Mirja, Nowinski Jana, Hung Chien-Wen, Neelsen Denis, Trad Ahmad, Tholey Andreas, Grötzinger Joachim, Lorenzen Inken Biochemical and biophysical research communications. Upd3--an ancestor of the four-helix bundle cytokines. 2013 Jun: 436(1) 66-72 https://www.ncbi.nlm.nih.gov/pubmed/23707937

201 Sánchez-San Martín Claudia, Nanda Soumya, Zheng Yan, Fields Whitney, Kielian Margaret Journal of virology. Cross-inhibition of chikungunya virus fusion and infection by alphavirus E1 domain III proteins. 2013 Jun: 87(13) 7680-7 https://www.ncbi.nlm.nih.gov/pubmed/23637415

202 Chen Zhian, Cui Yanfang, Leong Yew Ann, Beddoe Travis, Yu Di International immunopharmacology. Efficient production of recombinant IL-21 proteins for pre-clinical studies by a two-step dilution refolding method. 2013 Jun: 16(3) 376-81 https://www.ncbi.nlm.nih.gov/pubmed/23474188

203 Kanao Tadayoshi, Kosaka Megumi, Yoshida Kyoya, Nakayama Hisayuki, Tamada Taro, Kuroki Ryota, Yamada Hidenori, Takada Jun, Kamimura Kazuo Acta crystallographica. Crystallization and preliminary X-ray diffraction analysis of tetrathionate hydrolase from Acidithiobacillus ferrooxidans. 2013 May: Section F, Structural biology and crystallization communications. 69(Pt 6) 692-4 https://www.ncbi.nlm.nih.gov/pubmed/23722856

204 Sweeting Braden, Brown Eric, Khan M Qasim, Chakrabartty Avijit, Pai Emil F PloS one. N-terminal helix-cap in α-helix 2 modulates β-state misfolding in rabbit and hamster prion proteins. 2013 May: 8(5) e63047 https://www.ncbi.nlm.nih.gov/pubmed/23675452

205 Ferrer Valéria Pereira, de Mari Thiago Lopes, Gremski Luiza Helena, Trevisan Silva Dilza, da Silveira Rafael Bertoni, Gremski Waldemiro, Chaim Olga Meiri, Senff-Ribeiro Andrea, Nader Helena Bonciani, Veiga Silvio Sanches PLoS neglected tropical diseases. A novel hyaluronidase from brown spider (Loxosceles intermedia) venom (Dietrich's Hyaluronidase): from cloning to functional characterization. 2013 May: 7(5) e2206 https://www.ncbi.nlm.nih.gov/pubmed/23658852

206 Mohammadi Hamid Shahbaz, Omidinia Eskandar Journal of chromatography. Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems. 2013 May: B, Analytical technologies in the biomedical and life sciences. 929 11-7 https://www.ncbi.nlm.nih.gov/pubmed/23644496

207 Yuan Sujuan, Xu Jian, Ge Ying, Yan Zheng, Du Guohua, Wang Nan PloS one. Prokaryotic ubiquitin-like ThiS fusion enhances the heterologous protein overexpression and aggregation in Escherichia coli. 2013 May: 8(4) e62529 https://www.ncbi.nlm.nih.gov/pubmed/23638106

208 Ogura Kenji, Kobashigawa Yoshihiro, Saio Tomohide, Kumeta Hiroyuki, Torikai Shinnosuke, Inagaki Fuyuhiko Protein engineering, design & selection : PEDS. Practical applications of hydrostatic pressure to refold proteins from inclusion bodies for NMR structural studies. 2013 May: 26(6) 409-16 https://www.ncbi.nlm.nih.gov/pubmed/23525046

209 Dong Haohao, Li Ping, Elliott Richard M, Dong Changjiang Journal of virology. Structure of Schmallenberg orthobunyavirus nucleoprotein suggests a novel mechanism of genome encapsidation. 2013 Apr: 87(10) 5593-601 https://www.ncbi.nlm.nih.gov/pubmed/23468499

210 Chong Mun Keat, Parthasarathy Krupakar, Yeo Hui Yu, Ng Mah Lee Protein engineering, design & selection : PEDS. Optimized sequential purification protocol for in vivo site-specific biotinylated full-length dengue virus capsid protein. 2013 Apr: 26(5) 377-87 https://www.ncbi.nlm.nih.gov/pubmed/23479673

211 Asad Sedigheh, Dabirmanesh Bahareh, Ghaemi Nasser, Etezad Seyed Masoud, Khajeh Khosro Molecular biotechnology. Studies on the refolding process of recombinant horseradish peroxidase. 2013 Apr: 54(2) 484-92 https://www.ncbi.nlm.nih.gov/pubmed/22872497

212 Ageitos José Manuel, Vallejo Juan Andrés, Serrat Manuel, Sánchez-Pérez Angeles, Villa Tomás G Molecular biotechnology. In vitro Ca(2+)-dependent maturation of milk-clotting recombinant Epr: minor extracellular protease: from Bacillus licheniformis. 2013 Apr: 54(2) 304-11 https://www.ncbi.nlm.nih.gov/pubmed/22736063

213 Zheng Hai, Miyakawa Takuya, Sawano Yoriko, Yamagoe Satoshi, Tanokura Masaru Acta crystallographica. Crystallization and preliminary X-ray analysis of human leukocyte cell-derived chemotaxin 2 (LECT2). 2013 Mar: Section F, Structural biology and crystallization communications. 69(Pt 3) 316-9 https://www.ncbi.nlm.nih.gov/pubmed/23519812

214 Ki Mi-Ran, Min Kiha, Kanth Bashistha Kumar, Lee Jinwon, Pack Seung Pil Bioprocess and biosystems engineering. Expression, reconstruction and characterization of codon-optimized carbonic anhydrase from Hahella chejuensis for CO2 sequestration application. 2013 Mar: 36(3) 375-81 https://www.ncbi.nlm.nih.gov/pubmed/22833227

215 Sadeghi Leila, Khajeh Khosro, Mollania Nasrin, Dabirmanesh Bahareh, Ranjbar Bijan Molecular biotechnology. Extra EF hand unit (DX) mediated stabilization and calcium independency of α-amylase. 2013 Feb: 53(3) 270-7 https://www.ncbi.nlm.nih.gov/pubmed/22407721

216 Gao Bingmiao, Zhangsun Dongting, Wu Yong, Lin Bo, Zhu Xiaopeng, Luo Sulan Applied microbiology and biotechnology. Expression, renaturation and biological activity of recombinant conotoxin GeXIVAWT. 2013 Jan: 97(3) 1223-30 https://www.ncbi.nlm.nih.gov/pubmed/22825834

217 Murugan Subathra, Ponsekaran Santhakumar, Kannivel Lingaraja, Mangamoori Lakshmi Narasu, Chandran Dev, Villuppanoor Alwar Srinivasan, Chakravarty Chandrani, Lal Sunil K Journal of virological methods. Recombinant haemagglutinin protein of highly pathogenic avian influenza A (H5N1) virus expressed in Pichia pastoris elicits a neutralizing antibody response in mice. 2012 Dec: 187(1) 20-5 https://www.ncbi.nlm.nih.gov/pubmed/22871671

218 Gao Mingming, Tong Yue, Tian Hong, Gao Xiangdong, Yao Wenbing Applied microbiology and biotechnology. Recombinant production of mGLP-1 by coupling of refolding and intein-mediated self-cleavage (CRIS). 2012 Nov: 96(5) 1283-90 https://www.ncbi.nlm.nih.gov/pubmed/22644527

219 Chhabra Gagan, Mathur Divya, Dixit Aparna, Garg Lalit C Protein expression and purification. Heterologous expression and biochemical characterization of recombinant alpha phosphoglucomutase from Mycobacterium tuberculosis H37Rv. 2012 Aug: 85(1) 117-24 https://www.ncbi.nlm.nih.gov/pubmed/22809717

220 Zhou Ying, Ma Xue, Hou Zheng, Xue Xiaoyan, Meng Jingru, Li Mingkai, Jia Min, Luo Xiaoxing Protein expression and purification. High cell density cultivation of recombinant Escherichia coli for prodrug of recombinant human GLPs production. 2012 Aug: 85(1) 38-43 https://www.ncbi.nlm.nih.gov/pubmed/22771632

221 Fould Benjamin, Lamamy Véronique, Guenin Sophie-Penelope, Ouvry Christine, Cogé Francis, Boutin Jean A, Ferry Gilles Protein science : a publication of the Protein Society. Mutagenic analysis in a pure molecular system shows that thioredoxin-interacting protein residue Cys247 is necessary and sufficient for a mixed disulfide formation with thioredoxin. 2012 Aug: 21(9) 1323-33 https://www.ncbi.nlm.nih.gov/pubmed/22760822

222 Okai Masahiko, Ohtsuka Jun, Asano Atsuko, Guo Linjun, Miyakawa Takuya, Miyazono Ken-ichi, Nakamura Akira, Okada Akitoshi, Zheng Hai, Kimura Kenzo, Nagata Koji, Tanokura Masaru Protein expression and purification. High pressure refolding, purification, and crystallization of flavin reductase from Sulfolobus tokodaii strain 7. 2012 Jul: 84(2) 214-8 https://www.ncbi.nlm.nih.gov/pubmed/22722101

223 Mine Shouhei, Ikegami Takahisa, Kawasaki Kazunori, Nakamura Tsutomu, Uegaki Koichi Protein expression and purification. Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii. 2012 Jul: 84(2) 265-9 https://www.ncbi.nlm.nih.gov/pubmed/22713621

224 Retnoningrum Debbie S, Pramesti H T, Santika P Y, Valerius O, Asjarie S, Suciati T Protein expression and purification. Codon optimization for high level expression of human bone morphogenetic protein-2 in Escherichia coli. 2012 Jul: 84(2) 188-94 https://www.ncbi.nlm.nih.gov/pubmed/22691543

225 Roschitzki-Voser Heidi, Schroeder Thilo, Lenherr Esther D, Frölich Franziska, Schweizer Andreas, Donepudi Mrudula, Ganesan Rajkumar, Mittl Peer R E, Baici Antonio, Grütter Markus G Protein expression and purification. Human caspases in vitro: expression, purification and kinetic characterization. 2012 Jul: 84(2) 236-46 https://www.ncbi.nlm.nih.gov/pubmed/22683476

226 Peraino Jaclyn Stromp, Hermanrud Christina E, Springett Lauren, Zhang Huiping, Li Guoying, Srinivasan Srimathi, Gusha Ashley, Sachs David H, Huang Christene A, Wang Zhirui Cellular immunology. Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15. 2012 Jul: 276(1-2) 162-7 https://www.ncbi.nlm.nih.gov/pubmed/22672968

227 Yu Wei-Hsuan, Huang Po-Tsang, Lou Kuo-Long, Yu Shuan-Su C, Lin Chen Journal of biomedical science. A smallest 6 kda metalloprotease, mini-matrilysin, in living world: a revolutionary conserved zinc-dependent proteolytic domain- helix-loop-helix catalytic zinc binding domain (ZBD). 2012 Jul: 19 54 https://www.ncbi.nlm.nih.gov/pubmed/22642296

228 Feng Yanye, Liu Lu, Wang Jipeng, Liu Jian, Hu Wei, Wang Xiaoning, Yang Zhong Protein expression and purification. Integrated refolding techniques for Schistosoma japonicum MTH1 overexpressed as inclusion bodies in Escherichia coli. 2012 Jul: 84(2) 181-7 https://www.ncbi.nlm.nih.gov/pubmed/22641057

229 Li Yanwen, Huang Yan, Hu Xuchu, Liu Xiaoquan, Ma Changling, Zhao Junhong, Wu Zhongdao, Xu Jin, Yu Xinbing Parasitology research. 41.5-kDa Cathepsin L protease from Clonorchis sinensis: expression, characterization, and serological reactivity of one excretory-secretory antigen. 2012 Jul: 111(2) 673-80 https://www.ncbi.nlm.nih.gov/pubmed/22406988

230 Puizdar Vida, Zajc Tajana, Zerovnik Eva, Renko Miha, Pieper Ursula, Eswar Narayanan, Sali Andrej, Dolenc Iztok, Turk Vito Biological chemistry. Biochemical characterization and structural modeling of human cathepsin E variant 2 in comparison to the wild-type protein. 2012 Jun: 393(3) 177-86 https://www.ncbi.nlm.nih.gov/pubmed/22718633

231 Mokhonov Vladislav V, Theendakara Veena P, Gribanova Yekaterina E, Ahmedli Novruz B, Farber Debora B PloS one. Sequence-specific binding of recombinant Zbed4 to DNA: insights into Zbed4 participation in gene transcription and its association with other proteins. 2012 Jun: 7(5) e35317 https://www.ncbi.nlm.nih.gov/pubmed/22693546

232 Kim Sung-Gun, Min Won-Ki, Rho Yong-Taek, Seo Jin-Ho Protein expression and purification. Electrostatic interaction-induced inclusion body formation of glucagon-like peptide-1 fused with ubiquitin and cationic tag. 2012 Jun: 84(1) 38-46 https://www.ncbi.nlm.nih.gov/pubmed/22564875

233 Li Juan, Zheng Lei, Li Pingli, Wang Fengshan Protein expression and purification. Intein-mediated expression, purification, and characterization of thymosin α1-thymopentin fusion peptide in Escherichia coli. 2012 Jun: 84(1) 1-8 https://www.ncbi.nlm.nih.gov/pubmed/22554820

234 Zhang Yan, Zhang Haiqing, Kong Yu, Feng Lijun Developmental and comparative immunology. Identification and characterization of an amphioxus matrix metalloproteinase homolog BbMMPL2 responding to bacteria challenge. 2012 Jun: 37(3-4) 371-80 https://www.ncbi.nlm.nih.gov/pubmed/22440860

235 Kabatek Aleksander, Veit Michael Viruses. Folding and oligomerization of the gp2b/gp3/gp4 spike proteins of equine arteritis virus in vitro. 2012 May: 4(3) 414-23 https://www.ncbi.nlm.nih.gov/pubmed/22590679

236 Roussel G, Matagne A, De Bolle X, Perpète E A, Michaux C Protein expression and purification. Purification, refolding and characterization of the trimeric Omp2a outer membrane porin from Brucella melitensis. 2012 May: 83(2) 198-204 https://www.ncbi.nlm.nih.gov/pubmed/22538317

237 Hazebrouck Stéphane, Guillon Blanche, Drumare Marie-Françoise, Paty Evelyne, Wal Jean-Michel, Bernard Hervé Molecular nutrition & food research. Trypsin resistance of the major peanut allergen Ara h 6 and allergenicity of the digestion products are abolished after selective disruption of disulfide bonds. 2012 Apr: 56(4) 548-57 https://www.ncbi.nlm.nih.gov/pubmed/22495983

238 Gao Ling, Zhang Chun, Li Lingling, Liang Lan, Deng Xuan, Wu Wutong, Su Zhiguo, Yu Rong The protein journal. Construction, expression and refolding of a bifunctional fusion protein consisting of C-terminal 12-residue of hirudin-PA and reteplase. 2012 Apr: 31(4) 328-36 https://www.ncbi.nlm.nih.gov/pubmed/22481531

239 Uchime Onyinyechukwu, Herrera Raul, Reiter Karine, Kotova Svetlana, Shimp Richard L, Miura Kazutoyo, Jones Dominique, Lebowitz Jacob, Ambroggio Xavier, Hurt Darrell E, Jin Albert J, Long Carole, Miller Louis H, Narum David L Eukaryotic cell. Analysis of the conformation and function of the Plasmodium falciparum merozoite proteins MTRAP and PTRAMP. 2012 Apr: 11(5) 615-25 https://www.ncbi.nlm.nih.gov/pubmed/22467743

240 Sarduy Emir Salas, Muñoz Aymara Cabrera, Trejo Sebastián Alejandro, de los A Chavéz Planes María Protein expression and purification. High-level expression of Falcipain-2 in Escherichia coli by codon optimization and auto-induction. 2012 Apr: 83(1) 59-69 https://www.ncbi.nlm.nih.gov/pubmed/22450163

241 Maîtrepierre Elodie, Sigoillot Maud, Le Pessot Laurence, Briand Loïc Protein expression and purification. Recombinant expression, in vitro refolding, and biophysical characterization of the N-terminal domain of T1R3 taste receptor. 2012 Apr: 83(1) 75-83 https://www.ncbi.nlm.nih.gov/pubmed/22450161

242 Lundqvist Lena C E, Jam Murielle, Barbeyron Tristan, Czjzek Mirjam, Sandström Corine Carbohydrate research. Substrate specificity of the recombinant alginate lyase from the marine bacteria Pseudomonas alginovora. 2012 Apr: 352 44-50 https://www.ncbi.nlm.nih.gov/pubmed/22429771

243 Kattamuri Chandramohan, Luedeke David M, Thompson Thomas B Protein expression and purification. Expression and purification of recombinant protein related to DAN and cerberus (PRDC). 2012 Apr: 82(2) 389-95 https://www.ncbi.nlm.nih.gov/pubmed/22381466

244 Dai Hui, Yin Shijin, Li Tian, Cao Zhijian, Ji Yonghua, Wu Yingliang, Li Wenxin Protein expression and purification. Recombinant expression, purification, and characterization of scorpion toxin BmαTX14. 2012 Apr: 82(2) 325-31 https://www.ncbi.nlm.nih.gov/pubmed/22343065

245 Pritchard David I, Telford Gary, Diab Miliza, Low Walter Biotechnology progress. Expression of a cGMP compatible Lucilia sericata insect serine proteinase debridement enzyme. 2012 Apr: 28(2) 567-72 https://www.ncbi.nlm.nih.gov/pubmed/22323388

246 Santos Clelton A, Beloti Lilian L, Toledo Marcelo A S, Crucello Aline, Favaro Marianna T P, Mendes Juliano S, Santiago André S, Azzoni Adriano R, Souza Anete P Protein expression and purification. A novel protein refolding protocol for the solubilization and purification of recombinant peptidoglycan-associated lipoprotein from Xylella fastidiosa overexpressed in Escherichia coli. 2012 Apr: 82(2) 284-9 https://www.ncbi.nlm.nih.gov/pubmed/22306742

247 Harrison Joseph S, Koellhoffer Jayne F, Chandran Kartik, Lai Jonathan R Biochemistry. Marburg virus glycoprotein GP2: pH-dependent stability of the ectodomain α-helical bundle. 2012 Mar: 51(12) 2515-25 https://www.ncbi.nlm.nih.gov/pubmed/22369502

248 Pimenta J, Dias F M V, Marques C C, Baptista M C, Vasques M I, Horta A E M, Barbas J P, Soares R, Mesquita P, Cabrita E, Fontes C M G A, Prates J A, Pereira R M Reproduction in domestic animals = Zuchthygiene. The prion-like protein Doppel enhances ovine spermatozoa fertilizing ability. 2012 Mar: 47(2) 196-202 https://www.ncbi.nlm.nih.gov/pubmed/21806689

249 Manque Patricio A, Woehlbier Ute, Lara Ana M, Tenjo Fernando, Alves João M, Buck Gregory A PloS one. Identification and characterization of a novel calcium-activated apyrase from Cryptosporidium parasites and its potential role in pathogenesis. 2012 Feb: 7(2) e31030 https://www.ncbi.nlm.nih.gov/pubmed/22363541

250 Saleem Muhammad, Prince Stephen M, Patel Hema, Chan Hannah, Feavers Ian M, Derrick Jeremy P Acta crystallographica. Refolding, purification and crystallization of the FrpB outer membrane iron transporter from Neisseria meningitidis. 2012 Feb: Section F, Structural biology and crystallization communications. 68(Pt 2) 231-5 https://www.ncbi.nlm.nih.gov/pubmed/22298007

251 Qian Lan, Zhu Shunying, Shen Jiaqing, Han Xiaodong, Gao Jin, Wu Mingyuan, Yu Yan, Lu Huili, Han Wei Protein expression and purification. Expression and purification of recombinant human Mig in Escherichia coli and its comparison with murine Mig. 2012 Feb: 82(1) 205-11 https://www.ncbi.nlm.nih.gov/pubmed/22266086

252 Li Ming, Fan Hua, Liu Jiahua, Wang Minhong, Wang Lili, Wang Chaozhan Applied biochemistry and biotechnology. High pH solubilization and chromatography-based renaturation and purification of recombinant human granulocyte colony-stimulating factor from inclusion bodies. 2012 Feb: 166(5) 1264-74 https://www.ncbi.nlm.nih.gov/pubmed/22212394

253 Ye Wei, Liu Jingwen, Wang Haiying, Wang Jufang, Wang Xiaoning Protein expression and purification. Cloning, expression, purification, and characterization of a glutamate-specific endopeptidase from Bacillus licheniformis. 2012 Feb: 82(1) 138-43 https://www.ncbi.nlm.nih.gov/pubmed/22202650

254 Dang Suying, Hong Tao, Bu Dawei, Tang Jian, Fan Jing, Zhang Wei Protein expression and purification. Optimized refolding and characterization of active C-terminal ADAMTS-18 fragment from inclusion bodies of Escherichia coli. 2012 Feb: 82(1) 32-6 https://www.ncbi.nlm.nih.gov/pubmed/22119775

255 Dong Fang, Fu Yongfeng, Li Xueping, Jiang Jianguo, Sun Jianhua, Cheng Xunjia Parasitology research. Cloning, expression, and characterization of salivary apyrase from Aedes albopictus. 2012 Feb: 110(2) 931-7 https://www.ncbi.nlm.nih.gov/pubmed/21842387

256 Ping Zhiguang, Shi Yi, Sun Yanling, Ma Liping, Wang Ming Acta crystallographica. Protein expression, crystallization and preliminary X-ray crystallographic analysis of chicken interferon-γ receptor α chain. 2012 Jan: Section F, Structural biology and crystallization communications. 68(Pt 1) 41-4 https://www.ncbi.nlm.nih.gov/pubmed/22232168

257 Yao Shugang, Qi Jianxun, Liu Jun, Chen Rong, Pan Xiaocheng, Li Xiaoying, Gao Feng, Xia Chun Acta crystallographica. Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope. 2012 Jan: Section F, Structural biology and crystallization communications. 68(Pt 1) 20-3 https://www.ncbi.nlm.nih.gov/pubmed/22232164

258 Basu Anindya, Leong Susanna Su Jan Journal of chromatography. High productivity chromatography refolding process for Hepatitis B Virus X (HBx) protein guided by statistical design of experiment studies. 2012 Jan: A. 1223 64-71 https://www.ncbi.nlm.nih.gov/pubmed/22209547

259 Shigeto Jun, Itoh Yoshitaka, Tsutsumi Yuji, Kondo Ryuichiro The FEBS journal. Identification of Tyr74 and Tyr177 as substrate oxidation sites in cationic cell wall-bound peroxidase from Populus alba L. 2012 Jan: 279(2) 348-57 https://www.ncbi.nlm.nih.gov/pubmed/22099451

260 Choi Seung Phill, Park Yong-Cheol, Lee JungHwa, Sim Sang Jun, Chang Ho-Nam Bioprocess and biosystems engineering. Effects of L-arginine on refolding of lysine-tagged human insulin-like growth factor 1 expressed in Escherichia coli. 2012 Jan: 35(1-2) 255-63 https://www.ncbi.nlm.nih.gov/pubmed/22002161

261 Li Suxia, Zhang Luosheng, Wu Qian, Xin Aijie, Zhao Jian, Fan Liqiang Enzyme and microbial technology. Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B. 2011 Nov: 49(2) 139-45 https://www.ncbi.nlm.nih.gov/pubmed/22112400

262 Singh Surinder M, Sharma Aparna, Upadhyay Arun K, Singh Anupam, Garg Lalit C, Panda Amulya K Protein expression and purification. Solubilization of inclusion body proteins using n-propanol and its refolding into bioactive form. 2011 Nov: 81(1) 75-82 https://www.ncbi.nlm.nih.gov/pubmed/21964443

263 Bruinzeel Wouter, Masure Stefan Protein expression and purification. Recombinant expression, purification and dimerization of the neurotrophic growth factor Artemin for in vitro and in vivo use. 2011 Nov: 81(1) 25-32 https://www.ncbi.nlm.nih.gov/pubmed/21907286

264 Baker Peter James, Chan Yan Mei, Hertel Moritz, Montclare Jin Kim Protein expression and purification. Characterization and identification of the protein partners of Fn3 domain in FnTm2. 2011 Nov: 81(1) 42-8 https://www.ncbi.nlm.nih.gov/pubmed/21907285

265 Zhang Jie, Ding Xin-Liang, Bian Zeng-Hui, Xia Yan-Kai, Wang Shou-Lin, Song Ling, Wang Xin-Ru Asian journal of andrology. Preparation and immunogenicity of tag-free recombinant human eppin. 2011 Nov: 13(6) 889-94 https://www.ncbi.nlm.nih.gov/pubmed/21892195

266 Stefan Alessandra, Conti Matteo, Rubboli Diego, Ravagli Lorenzo, Presta Enrica, Hochkoeppler Alejandro Journal of biotechnology. Overexpression and purification of the recombinant diphtheria toxin variant CRM197 in Escherichia coli. 2011 Nov: 156(4) 245-52 https://www.ncbi.nlm.nih.gov/pubmed/21889551

267 Uhlig Christiane, Kabisch Johannes, Palm Gottfried J, Valentin Klaus, Schweder Thomas, Krell Andreas Cryobiology. Heterologous expression, refolding and functional characterization of two antifreeze proteins from Fragilariopsis cylindrus (Bacillariophyceae). 2011 Nov: 63(3) 220-8 https://www.ncbi.nlm.nih.gov/pubmed/21884691

268 Zhang R C, Chen J, Li C H, Lu X J, Shi Y H Fish & shellfish immunology. Prokaryotic expression, purification, and refolding of leukocyte cell-derived chemotaxin 2 and its effect on gene expression of head kidney-derived macrophages of a teleost fish, ayu (Plecoglossus altivelis). 2011 Nov: 31(6) 911-8 https://www.ncbi.nlm.nih.gov/pubmed/21871568

269 Zhang Nianzhi, Qi Jianxun, Feng Sijia, Gao Feng, Liu Jun, Pan Xiaocheng, Chen Rong, Li Qirun, Chen Zhaosan, Li Xiaoying, Xia Chun, Gao George F Journal of virology. Crystal structure of swine major histocompatibility complex class I SLA-1 0401 and identification of 2009 pandemic swine-origin influenza A H1N1 virus cytotoxic T lymphocyte epitope peptides. 2011 Oct: 85(22) 11709-24 https://www.ncbi.nlm.nih.gov/pubmed/21900158

270 Pham Le Thanh Mai, Kim Su Jin, Song Bong Keun, Kim Yong Hwan Protein expression and purification. Optimized refolding and characterization of S-peroxidase (CWPO_C of Populus alba) expressed in E. coli. 2011 Oct: 80(2) 268-73 https://www.ncbi.nlm.nih.gov/pubmed/21856428

271 Wang Xiao-Tao, Mion Brian, Aherne Aileen, Engel Paul C Biochimica et biophysica acta. Molecular recruitment as a basis for negative dominant inheritance? propagation of misfolding in oligomers of IMPDH1, the mutated enzyme in the RP10 form of retinitis pigmentosa. 2011 Oct: 1812(11) 1472-6 https://www.ncbi.nlm.nih.gov/pubmed/21791244

272 Song Fei, Zhang Xin, Li Yifei, Ru Qiang, Ren Xiaobai, Xia Bin, Chen Zhi-Nan Acta biochimica et biophysica Sinica. An efficient method for refolding the extracellular portion of CD147 from the total bacterial lysate. 2011 Oct: 43(11) 900-8 https://www.ncbi.nlm.nih.gov/pubmed/21764804

273 Tsuji Isamu, Mastui Hideki, Ito Tatsuo, Kurokawa Tomofumi, Shintani Yasushi Protein expression and purification. L-cysteine-enhanced renaturation of bioactive soluble tumor necrosis factor ligand family member LIGHT from inclusion bodies in Escherichia coli. 2011 Oct: 80(2) 239-45 https://www.ncbi.nlm.nih.gov/pubmed/21745574

274 Cothran Amber, St John Richard J, Schmelzer Charles H, Pizarro Shelly A Biotechnology progress. High-pressure refolding of human vascular endothelial growth factor (VEGF) recombinantly expressed in bacterial inclusion bodies: refolding optimization, and feasibility assessment. 2011 Oct: 27(5) 1273-81 https://www.ncbi.nlm.nih.gov/pubmed/21608142

275 Thammasittirong Anon, Dechklar Manasave, Leetachewa Somphob, Pootanakit Kusol, Angsuthanasombat Chanan Applied and environmental microbiology. Aedes aegypti membrane-bound alkaline phosphatase expressed in Escherichia coli retains high-affinity binding for Bacillus thuringiensis Cry4Ba toxin. 2011 Sep: 77(19) 6836-40 https://www.ncbi.nlm.nih.gov/pubmed/21856837

276 Ma Brian J, Kane Kevin P Journal of immunology (Baltimore, Md. Recognition of class I MHC by a rat Ly49 NK cell receptor is dependent on the identity of the P2 anchor amino acid of bound peptide. 2011 Sep: : 1950). 187(6) 3267-76 https://www.ncbi.nlm.nih.gov/pubmed/21841133

277 Di Maro Antimo, Farisei Francesca, Panichi Daniela, Severino Valeria, Bruni Natalia, Ficca Anna Grazia, Ferranti Pasquale, Capuzzi Valeria, Tedeschi Francesca, Poerio Elia Planta. WCI, a novel wheat chymotrypsin inhibitor: purification, primary structure, inhibitory properties and heterologous expression. 2011 Sep: 234(4) 723-35 https://www.ncbi.nlm.nih.gov/pubmed/21617989

278 Zhang Yujie, Xu Tingting, Chen Qiujia, Wang Bing, Liu Jinsong Protein expression and purification. Expression, purification, and refolding of active human and mouse secreted group IIE phospholipase A₂. 2011 Sep: 80(1) 68-73 https://www.ncbi.nlm.nih.gov/pubmed/21605679

279 Zhang Zhen, Xu Xiaoling, Zhang Xiaoren, Chen Xiqian, Chen Qingge, Dong Lina, Hu Zhiming, Li Jinlong, Gao Jimin Acta oncologica (Stockholm, Sweden). The therapeutic potential of SA-sCD40L in the orthotopic model of superficial bladder cancer. 2011 Sep: 50(7) 1111-8 https://www.ncbi.nlm.nih.gov/pubmed/21247263

280 Alibolandi Mona, Mirzahoseini Hasan Biotechnology research international. Purification and Refolding of Overexpressed Human Basic Fibroblast Growth Factor in Escherichia coli. 2011 Aug: 2011 973741 https://www.ncbi.nlm.nih.gov/pubmed/21837279

281 Smout Michael J, Mulvenna Jason P, Jones Malcolm K, Loukas Alex Protein expression and purification. Expression, refolding and purification of Ov-GRN-1, a granulin-like growth factor from the carcinogenic liver fluke, that causes proliferation of mammalian host cells. 2011 Aug: 79(2) 263-70 https://www.ncbi.nlm.nih.gov/pubmed/21757010

282 Argyri Letta, Skamnaki Vassiliki, Stratikos Efstratios, Chroni Angeliki Protein expression and purification. A simple approach for human recombinant apolipoprotein E4 expression and purification. 2011 Aug: 79(2) 251-7 https://www.ncbi.nlm.nih.gov/pubmed/21712092

283 Dutta Sruti, Choudhury Debi, Dattagupta Jiban K, Biswas Sampa The FEBS journal. C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism. 2011 Aug: 278(17) 3012-24 https://www.ncbi.nlm.nih.gov/pubmed/21707922

284 Rodríguez Lorena, Martínez Beatriz, Zhou Yuan, Rodríguez Ana, Donovan David M, García Pilar BMC microbiology. Lytic activity of the virion-associated peptidoglycan hydrolase HydH5 of Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88. 2011 Aug: 11 138 https://www.ncbi.nlm.nih.gov/pubmed/21682850

285 Arroyo M, García-Hidalgo J, Villalón M, de Eugenio L, Hormigo D, Acebal C, García J L, Prieto M A, de la Mata Isabel Journal of industrial microbiology & biotechnology. Characterization of a novel immobilized biocatalyst obtained by matrix-assisted refolding of recombinant polyhydroxyoctanoate depolymerase from Pseudomonas putida KT2442 isolated from inclusion bodies. 2011 Aug: 38(9) 1203-9 https://www.ncbi.nlm.nih.gov/pubmed/21104001

286 Volontè Federica, Piubelli Luciano, Pollegioni Loredano Microbial cell factories. Optimizing HIV-1 protease production in Escherichia coli as fusion protein. 2011 Jul: 10 53 https://www.ncbi.nlm.nih.gov/pubmed/21718537

287 Mager Frauke, Gessmann Dennis, Nussberger Stephan, Zeth Kornelius The Journal of membrane biology. Functional refolding and characterization of two Tom40 isoforms from human mitochondria. 2011 Jul: 242(1) 11-21 https://www.ncbi.nlm.nih.gov/pubmed/21717124

288 Jiang Jing, Deng Leixiu, He Lichun, Liu Haiting, Wang Changhai International journal of molecular medicine. Expression, purification, refolding, and characterization of octreotide-interleukin-2: a chimeric tumor-targeting protein. 2011 Jul: 28(4) 549-56 https://www.ncbi.nlm.nih.gov/pubmed/21687929

289 Garrido Francisco, Estrela Sylvie, Alves Claudia, Sánchez-Pérez Gabino F, Sillero Antonio, Pajares María A Protein expression and purification. Refolding and characterization of methionine adenosyltransferase from Euglena gracilis. 2011 Jul: 79(1) 128-36 https://www.ncbi.nlm.nih.gov/pubmed/21605677

290 Rodriguez-Cabrera Norma A, Regalado C, Garcia-Almendarez Blanca E Journal of agricultural and food chemistry. Cloning, heterologous expression and properties of a recombinant active turnip peroxidase. 2011 Jul: 59(13) 7120-6 https://www.ncbi.nlm.nih.gov/pubmed/21591783

291 Watson Aleksandra A, Christou Charita M, O'Callaghan Christopher A Protein expression and purification. Expression, purification and crystallization of the human UL16-binding protein ULBP1. 2011 Jul: 79(1) 44-8 https://www.ncbi.nlm.nih.gov/pubmed/21575723

292 Velmurugan Natarajan, Kim Hee Sung, Jeong Ki Jun Protein expression and purification. Enhanced production of human FcγRIIa receptor by high cell density cultivation of Escherichia coli. 2011 Jul: 79(1) 60-5 https://www.ncbi.nlm.nih.gov/pubmed/21511037

293 Chu Teng-Ping J, Yuann Jeu-Ming P Biotechnology and applied biochemistry. Expression, purification, and characterization of protective MPT64 antigen protein and identification of its multimers isolated from nontoxic Mycobacterium tuberculosis H37Ra. 2011 Jun: 58(3) 185-9 https://www.ncbi.nlm.nih.gov/pubmed/21679242

294 Belak Zachery R, Nair Manoj, Ovsenek Nick Biotechnology and applied biochemistry. Parameters for effective in vitro production of zinc finger nucleic acid-binding proteins. 2011 Jun: 58(3) 166-74 https://www.ncbi.nlm.nih.gov/pubmed/21679240

295 Kong Bo, Guo Grace L PloS one. Enhanced in vitro refolding of fibroblast growth factor 15 with the assistance of SUMO fusion partner. 2011 Jun: 6(5) e20307 https://www.ncbi.nlm.nih.gov/pubmed/21655243

296 Bellot Gaëtan, Pascal Robert, Mendre Christiane, Urbach Serge, Mouillac Bernard, Déméné Hélène Protein expression and purification. Expression, purification and NMR characterization of the cyclic recombinant form of the third intracellular loop of the vasopressin type 2 receptor. 2011 Jun: 78(2) 131-8 https://www.ncbi.nlm.nih.gov/pubmed/21575724

297 Kim Sung-Gun, Shin So-Yeon, Park Yong-Cheol, Shin Chul-Soo, Seo Jin-Ho Protein expression and purification. Production and solid-phase refolding of human glucagon-like peptide-1 using recombinant Escherichia coli. 2011 Jun: 78(2) 197-203 https://www.ncbi.nlm.nih.gov/pubmed/21421051

298 Kumar Kishore, Bhargava Prachi, Roy Uma Applied biochemistry and biotechnology. In vitro refolding of triosephosphate isomerase from L. donovani. 2011 Jun: 164(7) 1207-14 https://www.ncbi.nlm.nih.gov/pubmed/21365180

299 Lopez Karlo M, Greenaway Frederick T Journal of neural transmission (Vienna, Austria : 1996). Identification of the copper-binding ligands of lysyl oxidase. 2011 Jun: 118(7) 1101-9 https://www.ncbi.nlm.nih.gov/pubmed/21190048

300 Balduino Keli N, Spencer Patrick J, Malavasi Natalia V, Chura-Chambi Rosa M, Lemke Laura S, Morganti Ligia Molecular biotechnology. Refolding by high pressure of a toxin containing seven disulfide bonds: bothropstoxin-1 from Bothrops jararacussu. 2011 Jun: 48(3) 228-34 https://www.ncbi.nlm.nih.gov/pubmed/21181456

301 Lu Tzu-Li, Chuang Jing-Yuan, Yang Jai-Sing, Chiu Shau-Ting, Hsiao Nai-Wan, Wu Mei-Chen, Wu Shih-Hsiung, Hsu Ching-Hsiang Evidence-based complementary and alternative medicine : eCAM. Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells. 2011 May: 2011 283747 https://www.ncbi.nlm.nih.gov/pubmed/21584270

302 Schwarz Christian K W, Tschapek Britta, Jumpertz Thorsten, Jenewein Stefan, Lecher Justin, Willbold Dieter, Panjikar Santosh, Holland I Barry, Smits Sander H J, Schmitt Lutz Acta crystallographica. Crystallization and preliminary X-ray crystallographic studies of an oligomeric species of a refolded C39 peptidase-like domain of the Escherichia coli ABC transporter haemolysin B. 2011 May: Section F, Structural biology and crystallization communications. 67(Pt 5) 630-3 https://www.ncbi.nlm.nih.gov/pubmed/21543878

303 Li Xiang, Leong Susanna Su Jan Journal of chromatography. A chromatography-focused bioprocess that eliminates soluble aggregation for bioactive production of a new antimicrobial peptide candidate. 2011 May: A. 1218(23) 3654-9 https://www.ncbi.nlm.nih.gov/pubmed/21530977

304 Kuhfahl Silke, Hauburger Anja, Thieme Tino, Groppe Jay, Ihling Christian, Tomic Sladjana, Schutkowski Mike, Sinz Andrea, Schwarz Elisabeth Biochemical and biophysical research communications. Identification of a core domain within the proregion of bone morphogenetic proteins that interacts with the dimeric, mature domain. 2011 May: 408(2) 300-5 https://www.ncbi.nlm.nih.gov/pubmed/21510924

305 Fang Hui-Min, Zhao Li, Lu Ping, Chen San-Jun, Bao Zhen-Xia, Qin Yun-Fei, Zhu Zhao-Yu, Zhao Jin-Mei, Mai Jia, Zhang Shou-Tao The protein journal. Modular design, expression and characterization of novel bifunctional mutants of fibrolase with combined platelet aggregation-inhibition and fibrinolytic activity. 2011 May: 30(4) 247-52 https://www.ncbi.nlm.nih.gov/pubmed/21479991

306 Moon Sung-Ung, Kang Jung-Mi, Kim Tong-Soo, Kong Yoon, Sohn Woon-Mok, Na Byoung-Kuk Experimental parasitology. Plasmodium vivax: collaborative roles for plasmepsin 4 and vivapains in hemoglobin hydrolysis. 2011 Apr: 128(2) 127-32 https://www.ncbi.nlm.nih.gov/pubmed/21334328

307 Gori Francesca, Mulinacci Barbara, Massai Lara, Avolio Carlo, Caragnano Mariantonietta, Peroni Elisa, Lori Silvia, Chelli Mario, Papini Anna Maria, Rovero Paolo, Lolli Francesco Journal of neuroimmunology. IgG and IgM antibodies to the refolded MOG(1-125) extracellular domain in humans. 2011 Apr: 233(1-2) 216-20 https://www.ncbi.nlm.nih.gov/pubmed/21215463

308 Narita Hirotaka, Nakagawa Atsushi, Yamamoto Yasunori, Sakisaka Toshiaki, Takai Yoshimi, Suzuki Mamoru Acta crystallographica. Refolding, crystallization and preliminary X-ray crystallographic study of the whole extracellular regions of nectins. 2011 Mar: Section F, Structural biology and crystallization communications. 67(Pt 3) 344-8 https://www.ncbi.nlm.nih.gov/pubmed/21393840

309 Zhao Qinghuan, Chen Weihong, Chen Yuanding, Zhang Liming, Zhang Jinping, Zhang Zhijun Bioconjugate chemistry. Self-assembled virus-like particles from rotavirus structural protein VP6 for targeted drug delivery. 2011 Mar: 22(3) 346-52 https://www.ncbi.nlm.nih.gov/pubmed/21338097

310 Golebiowski Filip M, Górecki Andrzej, Bonarek Piotr, Dziedzicka-Wasylewska Marta Protein expression and purification. Efficient overexpression and purification of active full-length human transcription factor Yin Yang 1 in Escherichia coli. 2011 Mar: 77(2) 198-206 https://www.ncbi.nlm.nih.gov/pubmed/21296159

311 Rozbeský Daniel, Kavan Daniel, Chmelík Josef, Novák Petr, Vaněk Ondřej, Bezouška Karel Protein expression and purification. High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli. 2011 Mar: 77(2) 178-84 https://www.ncbi.nlm.nih.gov/pubmed/21284957

312 Basu Anindya, Chen Wei Ning, Leong Susanna Su Jan Applied microbiology and biotechnology. A rational design for hepatitis B virus X protein refolding and bioprocess development guided by second virial coefficient studies. 2011 Mar: 90(1) 181-91 https://www.ncbi.nlm.nih.gov/pubmed/21212947

313 Ma Yu-Bin, Zhang Zhi-Feng, Shao Ming-Yu, Kang Kyoung-Ho, Tan Zhi, Li Jin-Long Marine biotechnology (New York, N. Sulfide:quinone oxidoreductase from echiuran worm Urechis unicinctus. 2011 Mar:Y.). 13(1) 93-107 https://www.ncbi.nlm.nih.gov/pubmed/20419499

314 Carvajal Patricia, Gibert Jordi, Campos Nefertiti, Lopera Oriol, Barberà Eduard, Torné Jose M, Santos Mireya Biotechnology progress. Activity of maize transglutaminase overexpressed in Escherichia coli inclusion bodies: an alternative to protein refolding. 2011 Feb: 27(1) 232-40 https://www.ncbi.nlm.nih.gov/pubmed/21312370

315 Jang Tae-ho, Park Hyun Ho Journal of biotechnology. Generalized semi-refolding methods for purification of the functional death domain superfamily. 2011 Feb: 151(4) 335-42 https://www.ncbi.nlm.nih.gov/pubmed/21237218

316 Botev Anne, Munter Lisa-Marie, Wenzel Ringo, Richter Luise, Althoff Veit, Ismer Jochen, Gerling Ulla, Weise Christoph, Koksch Beate, Hildebrand Peter W, Bittl Robert, Multhaup Gerd Biochemistry. The amyloid precursor protein C-terminal fragment C100 occurs in monomeric and dimeric stable conformations and binds γ-secretase modulators. 2011 Feb: 50(5) 828-35 https://www.ncbi.nlm.nih.gov/pubmed/21186781

317 Dworeck Tamara, Petri Anne-Kathrin, Muhammad Noor, Fioroni Marco, Schwaneberg Ulrich Protein expression and purification. FhuA deletion variant Δ1-159 overexpression in inclusion bodies and refolding with Polyethylene-Poly(ethylene glycol) diblock copolymer. 2011 Feb: 77(1) 75-9 https://www.ncbi.nlm.nih.gov/pubmed/21168506

318 McConnell Michael J, Pachón Jerónimo Protein expression and purification. Expression, purification, and refolding of biologically active Acinetobacter baumannii OmpA from Escherichia coli inclusion bodies. 2011 Feb: 77(1) 98-103 https://www.ncbi.nlm.nih.gov/pubmed/21130880

319 Simeonov Peter, Berger-Hoffmann Renate, Hoffmann Ralf, Sträter Norbert, Zuchner Thole Protein engineering, design & selection : PEDS. Surface supercharged human enteropeptidase light chain shows improved solubility and refolding yield. 2011 Feb: 24(3) 261-8 https://www.ncbi.nlm.nih.gov/pubmed/21084283

320 Jinyong Zhang, Xiaoli Zhang, Weijun Zhang, Ying Guo, Gang Guo, Xuhu Mao, Quanming Zou Molecular biology reports. Fusion expression and immunogenicity of Bordetella pertussis PTS1-FHA protein: implications for the vaccine development. 2011 Feb: 38(3) 1957-63 https://www.ncbi.nlm.nih.gov/pubmed/20878241

321 Lantez Violaine, Dalle Karen, Charrel Rémi, Baronti Cécile, Canard Bruno, Coutard Bruno PLoS neglected tropical diseases. Comparative production analysis of three phlebovirus nucleoproteins under denaturing or non-denaturing conditions for crystallographic studies. 2011 Jan: 5(1) e936 https://www.ncbi.nlm.nih.gov/pubmed/21245924

322 Kaplan Ondřej, Bezouška Karel, Plíhal Ondřej, Ettrich Rüdiger, Kulik Natallia, Vaněk Ondřej, Kavan Daniel, Benada Oldřich, Malandra Anna, Sveda Ondřej, Veselá Alicja B, Rinágelová Anna, Slámová Kristýna, Cantarella Maria, Felsberg Jürgen, Dušková Jarmila, Dohnálek Jan, Kotik Michael, Křen Vladimír, Martínková Ludmila BMC biotechnology. Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10. 2011 Jan: 11 2 https://www.ncbi.nlm.nih.gov/pubmed/21210990

323 Goto Hiroaki, Okuda Satohiro, Mizukami Akane, Mori Hitoshi, Sasaki Narie, Kurihara Daisuke, Higashiyama Tetsuya Plant & cell physiology. Chemical visualization of an attractant peptide, LURE. 2011 Jan: 52(1) 49-58 https://www.ncbi.nlm.nih.gov/pubmed/21149297

324 Mahmood Anjum, Srivastava Shubhra, Tripathi Sarita, Ansari Mairaj Ahmed, Owais Mohammad, Arora Ashish The FEBS journal. Molecular characterization of secretory proteins Rv3619c and Rv3620c from Mycobacterium tuberculosis H37Rv. 2011 Jan: 278(2) 341-53 https://www.ncbi.nlm.nih.gov/pubmed/21134129

325 Khurana Surender, Verma Swati, Verma Nitin, Crevar Corey J, Carter Donald M, Manischewitz Jody, King Lisa R, Ross Ted M, Golding Hana Journal of virology. Bacterial HA1 vaccine against pandemic H5N1 influenza virus: evidence of oligomerization, hemagglutination, and cross-protective immunity in ferrets. 2011 Jan: 85(3) 1246-56 https://www.ncbi.nlm.nih.gov/pubmed/21084473

326 Tsai Inn-Ho, Wang Ying-Ming, Hseu Ming-Jhy Biochimie. Mutagenesis analyses explore residues responsible for the neurotoxic and anticoagulant activities of Trimucrotoxin, a pit-viper venom Asn6-phospholipase A2. 2011 Jan: 93(2) 277-85 https://www.ncbi.nlm.nih.gov/pubmed/20920547

327 Potala Sirisha, Verma Rama S Molecular biology reports. Targeting head and neck squamous cell carcinoma using a novel fusion toxin-diphtheria toxin/HN-1. 2011 Jan: 38(2) 1389-97 https://www.ncbi.nlm.nih.gov/pubmed/20814829

328 Liu Yang, Li Xiao-Li, Zhang Dong-Dong, Wang Qiang, Liu Yong, Liu Dong-Mei, Wu Chu-Tse, Cui Chun-Ping Molecular biotechnology. Production of hepatopoietin Cn in high-cell-density cultures of recombinant Escherichia coli and detection of its antioxygen activity. 2011 Jan: 47(2) 111-9 https://www.ncbi.nlm.nih.gov/pubmed/20676803

329 Jia Yan, Liu Hui, Bao Wei, Weng Meizhi, Chen Wei, Cai Yongjun, Zheng Zhongliang, Zou Guolin FEBS letters. Functional analysis of propeptide as an intramolecular chaperone for in vivo folding of subtilisin nattokinase. 2010 Dec: 584(23) 4789-96 https://www.ncbi.nlm.nih.gov/pubmed/21074529

330 Magellan Hervé, Drujon Thierry, Thellend Annie, Piffeteau Annie, Becker Hubert F BMC research notes. Expression in E. coli and characterization of the catalytic domain of Botrytis cinerea chitin synthase. 2010 Dec: 3 299 https://www.ncbi.nlm.nih.gov/pubmed/21070667

331 Plesniak Leigh A, Mahalakshmi Radhakrishnan, Rypien Candace, Yang Yuan, Racic Jasmina, Marassi Francesca M Biochimica et biophysica acta. Expression, refolding, and initial structural characterization of the Y. pestis Ail outer membrane protein in lipids. 2010 Dec: 1808(1) 482-9 https://www.ncbi.nlm.nih.gov/pubmed/20883662

332 Lee I-Liang, Li Pei-Shan, Yu Wei-Lin, Shen Hsin-Hsin Protein expression and purification. Prokaryotic expression, refolding, and purification of functional human vascular endothelial growth factor isoform 165: purification procedures and refolding conditions revisited. 2010 Dec: 76(1) 54-8 https://www.ncbi.nlm.nih.gov/pubmed/20826215

333 Panchal Manoj, Muralidhar Kambadur Preparative biochemistry & biotechnology. Purification and biological characterization of bacterially expressed recombinant buffalo prolactin. 2010 Nov: 40(4) 276-85 https://www.ncbi.nlm.nih.gov/pubmed/21108131

334 Jeong Myoung Seok, Kang Chang Soo, Han Yeon Soo, Bang In Seok Journal of microbiology (Seoul, Korea). Expression, purification, and characterization of recombinant fibulin-5 in a prokaryote expression system. 2010 Nov: 48(5) 695-700 https://www.ncbi.nlm.nih.gov/pubmed/21046350

335 Zhang Yanhong, Ma Yinsun, Yang Mingying, Min Sijia, Yao Juming, Zhu Liangjun Protein expression and purification. Expression, purification, and refolding of a recombinant human bone morphogenetic protein 2 in vitro. 2010 Nov: 75(2) 155-60 https://www.ncbi.nlm.nih.gov/pubmed/20691269

336 Wu Bingyuan, Nemeth Jennifer F, Janecki Dariusz J, Jones Brian, Obmolova Galina, Malia Thomas J, Baker Audrey, Bethea Deidra, Elloso M Merle, Naso Michael, Taudte Susann Cytokine. Expression, refolding and purification of a human interleukin-17A variant. 2010 Nov: 53(1) 107-14 https://www.ncbi.nlm.nih.gov/pubmed/20674388

337 Baumann Lars, Beck-Sickinger Annette G Biopolymers. Identification of a potential modification site in human stromal cell-derived factor-1. 2010 Nov: 94(6) 771-8 https://www.ncbi.nlm.nih.gov/pubmed/20564039

338 Sun Weimin, Dai Xinxian, Zheng Yanpeng, Wang Jianwei, Hou Lingling, Du Juan, Hu Hong-gang Protein expression and purification. On-column refolding purification of DT389-hIL13 recombinant protein expressed in Escherichia coli. 2010 Oct: 75(1) 83-8 https://www.ncbi.nlm.nih.gov/pubmed/20851185

339 Lee Carol M Y, McGuire Helen, Basten Antony, King Cecile, Christ Daniel Journal of immunological methods. Expression, purification and characterization of recombinant interleukin-21. 2010 Oct: 362(1-2) 185-9 https://www.ncbi.nlm.nih.gov/pubmed/20800065

340 Takehara Sayaka, Zhang Juan, Yang Xiaoyan, Takahashi Nobuyuki, Mikami Bunzo, Onda Maki Journal of molecular biology. Refolding and polymerization pathways of neuroserpin. 2010 Oct: 403(5) 751-62 https://www.ncbi.nlm.nih.gov/pubmed/20691191

341 Luo Xiao, Liu Ya-Li, Layfield Sharon, Shao Xiao-Xia, Bathgate Ross A D, Wade John D, Guo Zhan-Yun Peptides. A simple approach for the preparation of mature human relaxin-3. 2010 Oct: 31(11) 2083-8 https://www.ncbi.nlm.nih.gov/pubmed/20688116

342 Chumanov Robert S, Burgess Richard R Protein expression and purification. Expression, purification, and refolding of active Nrf2 transcription factor fused to protein transduction TAT tag. 2010 Oct: 74(2) 280-8 https://www.ncbi.nlm.nih.gov/pubmed/20600948

343 Moreira Susana, Castanheira Pedro, Casal Margarida, Faro Carlos, Gama Miguel Protein expression and purification. Expression of the functional carbohydrate-binding module (CBM) of human laforin. 2010 Oct: 74(2) 169-74 https://www.ncbi.nlm.nih.gov/pubmed/20600946

344 Cheng Xiawei, Lu Wuguang, Zhang Shuangquan, Cao Peng Amino acids. Expression and purification of antimicrobial peptide CM4 by Npro fusion technology in E. coli. 2010 Oct: 39(5) 1545-52 https://www.ncbi.nlm.nih.gov/pubmed/20512388

345 Schröder-Tittmann Kathrin, Bosse-Doenecke Eva, Reedtz-Runge Steffen, Ihling Christian, Sinz Andrea, Tittmann Kai, Rudolph Rainer Biochemistry. Recombinant expression, in vitro refolding, and biophysical characterization of the human glucagon-like peptide-1 receptor. 2010 Sep: 49(36) 7956-65 https://www.ncbi.nlm.nih.gov/pubmed/20690636

346 Bussières Laetitia, Bordas-Le Floch Véronique, Bulder Ingrid, Chabre Henri, Nony Emmanuel, Lautrette Aurélie, Berrouet Christelle, Nguefeu Yvette, Horiot Stéphane, Baron-Bodo Véronique, Van Overtvelt Laurence, De Conti Anne Marie, Schlegel Anne, Maguet Nicolas, Mouz Nicolas, Lemoine Pierrick, Batard Thierry, Moingeon Philippe International archives of allergy and immunology. Recombinant fusion proteins assembling Der p 1 and Der p 2 allergens from Dermatophagoides pteronyssinus. 2010 Sep: 153(2) 141-51 https://www.ncbi.nlm.nih.gov/pubmed/20407270

347 Zhang Hongbin, Wu Jie, Zhang Yu, Fu Na, Wang Jie, Zhao Shujin Molecular biology reports. Optimized procedure for expression and renaturation of recombinant human bone morphogenetic protein-2 at high protein concentrations. 2010 Sep: 37(7) 3089-95 https://www.ncbi.nlm.nih.gov/pubmed/19823948

348 Cerutti Nichole, Mendelow Barry V, Napier Grant B, Papathanasopoulos Maria A, Killick Mark, Khati Makobetsa, Stevens Wendy, Capovilla Alexio The Journal of biological chemistry. Stabilization of HIV-1 gp120-CD4 receptor complex through targeted interchain disulfide exchange. 2010 Aug: 285(33) 25743-52 https://www.ncbi.nlm.nih.gov/pubmed/20538591

349 Son Young-Jin, Bae Ji-Young, Chong Seon-Ha, Lee Hui Sun, Mo Sang Hyun, Kim Tae Yoon, Choe Han Applied biochemistry and biotechnology. Expression, high cell density culture and purification of recombinant EC-SOD in Escherichia coli. 2010 Aug: 162(6) 1585-98 https://www.ncbi.nlm.nih.gov/pubmed/20467833

350 Zhang Shou-Tao, Lu Ping, Qin Yun-Fei, Chen San-Jun, Guo Ai-Guang Applied biochemistry and biotechnology. Cloning and identification of a novel P-II class snake venom metalloproteinase from Gloydius halys. 2010 Aug: 162(5) 1391-402 https://www.ncbi.nlm.nih.gov/pubmed/20174888

351 Potala Sirisha, Verma Rama Shanker Applied biochemistry and biotechnology. A novel fusion protein diphtheria toxin-stem cell factor (DT-SCF)-purification and characterization. 2010 Aug: 162(5) 1258-69 https://www.ncbi.nlm.nih.gov/pubmed/20084469

352 Shaikhibrahim Zaki, Rahaman Hamidur, Wittung-Stafshede Pernilla, Björklund Stefan Proceedings of the National Academy of Sciences of the United States of America. Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation. 2010 Aug: 106(49) 20728-33 https://www.ncbi.nlm.nih.gov/pubmed/19934057

353 Khurana Surender, Verma Swati, Verma Nitin, Crevar Corey J, Carter Donald M, Manischewitz Jody, King Lisa R, Ross Ted M, Golding Hana PloS one. Properly folded bacterially expressed H1N1 hemagglutinin globular head and ectodomain vaccines protect ferrets against H1N1 pandemic influenza virus. 2010 Jul: 5(7) e11548 https://www.ncbi.nlm.nih.gov/pubmed/20634959

354 Kanao Tadayoshi, Matsumoto Chie, Shiraga Kumiko, Yoshida Kyoya, Takada Jun, Kamimura Kazuo FEMS microbiology letters. Recombinant tetrathionate hydrolase from Acidithiobacillus ferrooxidans requires exposure to acidic conditions for proper folding. 2010 Jul: 309(1) 43-7 https://www.ncbi.nlm.nih.gov/pubmed/20546308

355 González-Rivera Christian, Gangwer Kelly A, McClain Mark S, Eli Ilyas M, Chambers Melissa G, Ohi Melanie D, Lacy D Borden, Cover Timothy L Biochemistry. Reconstitution of Helicobacter pylori VacA toxin from purified components. 2010 Jul: 49(27) 5743-52 https://www.ncbi.nlm.nih.gov/pubmed/20527875

356 Fraga Tatiana R, Chura-Chambi Rosa M, Gonçales Amane P, Morais Zenaide M, Vasconcellos Sílvio A, Morganti Ligia, Martins Elizabeth A L Journal of biotechnology. Refolding of the recombinant protein OmpA70 from Leptospira interrogans from inclusion bodies using high hydrostatic pressure and partial characterization of its immunological properties. 2010 Jul: 148(2-3) 156-62 https://www.ncbi.nlm.nih.gov/pubmed/20450943

357 Fradkin Amber Haynes, Boand Carl S, Eisenberg Stephen P, Rosendahl Mary S, Randolph Theodore W Biotechnology progress. Recombinant murine growth hormone from E. coli inclusion bodies: expression, high-pressure solubilization and refolding, and characterization of activity and structure. 2010 Jun: 26(3) 743-9 https://www.ncbi.nlm.nih.gov/pubmed/20196161

358 Hrmova Maria, Stone Bruce A, Fincher Geoffrey B Glycoconjugate journal. High-yield production, refolding and a molecular modelling of the catalytic module of (1,3)-beta-D-glucan (curdlan) synthase from Agrobacterium sp. 2010 May: 27(4) 461-76 https://www.ncbi.nlm.nih.gov/pubmed/20473714

359 Sharapova Olga A, Pozdnykova Natalia V, Laurinavichyute Daniela K, Yurkova Maria S, Posypanova Galina A, Fedorov Alexey N, Severin Sergey E, Severin Evgeny S Protein expression and purification. High-efficient expression, refolding and purification of functional recombinant C-terminal fragment of human alpha-fetoprotein. 2010 May: 73(1) 31-5 https://www.ncbi.nlm.nih.gov/pubmed/20363333

360 Zhao Xiangzhong, Liu Ming, Wu Ning, Ding Lili, Liu Haizhou, Lin Xiukun Protein expression and purification. Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro. 2010 May: 72(2) 262-6 https://www.ncbi.nlm.nih.gov/pubmed/20363330

361 Petrov Stefan, Nacheva Genoveva, Ivanov Ivan Protein expression and purification. Purification and refolding of recombinant human interferon-gamma in urea-ammonium chloride solution. 2010 May: 73(1) 70-3 https://www.ncbi.nlm.nih.gov/pubmed/20363329

362 Tu Zhidan, Cohen Mark, Bu Hong, Lin Feng The American journal of pathology. Tissue distribution and functional analysis of Sushi domain-containing protein 4. 2010 May: 176(5) 2378-84 https://www.ncbi.nlm.nih.gov/pubmed/20348246

363 Niv-Spector Leonora, Shpilman Michal, Grupi Asaf, Gertler Arieh Molecular genetics and metabolism. The obese phenotype-inducing N82K mutation in human leptin disrupts receptor-binding and biological activity. 2010 May: 100(2) 193-7 https://www.ncbi.nlm.nih.gov/pubmed/20307995

364 Pizarro Shelly A, Gunson Jane, Field Matthew J, Dinges Rachel, Khoo Stefanie, Dalal Milind, Lee Michael, Kaleas Kimberly A, Moiseff Kathryn, Garnick Susan, Reilly Dorothea E, Laird Michael W, Schmelzer Charles H Protein expression and purification. High-yield expression of human vascular endothelial growth factor VEGF(165) in Escherichia coli and purification for therapeutic applications. 2010 May: 72(2) 184-93 https://www.ncbi.nlm.nih.gov/pubmed/20302942

365 von Einem S, Schwarz E, Rudolph R Protein expression and purification. A novel TWO-STEP renaturation procedure for efficient production of recombinant BMP-2. 2010 May: 73(1) 65-9 https://www.ncbi.nlm.nih.gov/pubmed/20302941

366 Lin Fusen, Ren Xiaoming, Guo Hong, Ding Qiong, Zheng Alan C Journal of virological methods. Expression, purification of the UL3 protein of herpes simplex virus type 1, and production of UL3 polyclonal antibody. 2010 May: 166(1-2) 72-6 https://www.ncbi.nlm.nih.gov/pubmed/20188759

367 Wang Jiarong, Tian Zigang, Teng Da, Yang Yalin, Hu Jiancheng, Wang Jianhua Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. Cloning, expression and characterization of Kunming mice lactoferrin and its N-lobe. 2010 May: 23(3) 523-30 https://www.ncbi.nlm.nih.gov/pubmed/20130963

368 Esfandiar Samaneh, Hashemi-Najafabadi Sameereh, Shojaosadati Seyed Abbas, Sarrafzadeh Shokuh Aazam, Pourpak Zahra Biotechnology and applied biochemistry. Purification and refolding of Escherichia coli-expressed recombinant human interleukin-2. 2010 Apr: 55(4) 209-14 https://www.ncbi.nlm.nih.gov/pubmed/20236092

369 Michalke Kerstin, Huyghe Céline, Lichière Julie, Gravière Marie-Eve, Siponen Marina, Sciara Giuliano, Lepaul Isabelle, Wagner Renaud, Magg Christine, Rudolph Rainer, Cambillau Christian, Desmyter Aline Analytical biochemistry. Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1. 2010 Apr: 401(1) 74-80 https://www.ncbi.nlm.nih.gov/pubmed/20175983

370 Fujiwara Yoshihiro, Aiki Yasuhiko, Yang Lijun, Takaiwa Fumio, Kosaka Akemi, Tsuji Noriko M, Shiraki Kentaro, Sekikawa Kenji Protein expression and purification. Extraction and purification of human interleukin-10 from transgenic rice seeds. 2010 Apr: 72(1) 125-30 https://www.ncbi.nlm.nih.gov/pubmed/20159041

371 Castanheira Pedro, Moreira Susana, Gama Miguel, Faro Carlos Protein expression and purification. Escherichia coli expression, refolding and characterization of human laforin. 2010 Mar: 71(2) 195-9 https://www.ncbi.nlm.nih.gov/pubmed/20152902

372 Koth Christopher M, Abdul-Manan Norzehan, Lepre Christopher A, Connolly Peter J, Yoo Sanghee, Mohanty Arun K, Lippke Judith A, Zwahlen Jacque, Coll Joyce T, Doran John D, Garcia-Guzman Miguel, Moore Jonathan M Biochemistry. Refolding and characterization of a soluble ectodomain complex of the calcitonin gene-related peptide receptor. 2010 Mar: 49(9) 1862-72 https://www.ncbi.nlm.nih.gov/pubmed/20099900

373 Beassoni Paola R, Berti Federico Pérez de, Otero Lisandro H, Risso Valeria A, Ferreyra Raul G, Lisa Angela T, Domenech Carlos E, Ermácora Mario R Protein expression and purification. Preparation and biophysical characterization of recombinant Pseudomonas aeruginosa phosphorylcholine phosphatase. 2010 Mar: 71(2) 153-9 https://www.ncbi.nlm.nih.gov/pubmed/20064618

374 Chen Xu, Wu Jueheng, Liu Hanning, He Zhenjian, Gu Minghui, Wang Ning, Ma Jianda, Hu Jieping, Xia Lei, He Haipeng, Yuan Jie, Li Jun, Li Luyuan, Li Mengfeng, Zhu Xun Protein science : a publication of the Protein Society. Approaches to efficient production of recombinant angiogenesis inhibitor rhVEGI-192 and characterization of its structure and antiangiogenic function. 2010 Mar: 19(3) 449-57 https://www.ncbi.nlm.nih.gov/pubmed/20052682

375 Rid Raphaela, Onder Kamil, Hawranek Thomas, Laimer Martin, Bauer Johann W, Holler Claudia, Simon-Nobbe Birgit, Breitenbach Michael Molecular immunology. Isolation and immunological characterization of a novel Cladosporium herbarum allergen structurally homologous to the alpha/beta hydrolase fold superfamily. 2010 Mar: 47(6) 1366-77 https://www.ncbi.nlm.nih.gov/pubmed/20022636

376 Zhu Shunyi, Gao Bin, Aumelas André, del Carmen Rodríguez Maria, Lanz-Mendoza Humberto, Peigneur Steve, Diego-Garcia Elia, Martin-Eauclaire Marie-France, Tytgat Jan, Possani Lourival D Biochimica et biophysica acta. MeuTXKbeta1, a scorpion venom-derived two-domain potassium channel toxin-like peptide with cytolytic activity. 2010 Feb: 1804(4) 872-83 https://www.ncbi.nlm.nih.gov/pubmed/20045493

377 Wang Yin-Jue, Liu Yong-Dong, Chen Jing, Hao Su-Juan, Hu Tao, Ma Guang-Hui, Su Zhi-Guo International journal of pharmaceutics. Efficient preparation and PEGylation of recombinant human non-glycosylated erythropoietin expressed as inclusion body in E. coli. 2010 Feb: 386(1-2) 156-64 https://www.ncbi.nlm.nih.gov/pubmed/19932158

378 Pan Weiwei, Ren Xiaoming, Guo Hong, Ding Qiong, Zheng Alan C Journal of virological methods. Expression, purification of herpes simplex virus type 1 UL4 protein, and production and characterization of UL4 polyclonal antibody. 2010 Feb: 163(2) 465-9 https://www.ncbi.nlm.nih.gov/pubmed/19917314

379 Arana Mercedes E, Powell Gary K, Edwards Lori L, Kunkel Thomas A, Petrovich Robert M Protein expression and purification. Refolding active human DNA polymerase nu from inclusion bodies. 2010 Feb: 70(2) 163-71 https://www.ncbi.nlm.nih.gov/pubmed/19853037

380 Tanyaratsrisakul S, Malainual N, Jirapongsananuruk O, Smith W A, Thomas W R, Piboonpocanun S International archives of allergy and immunology. Structural and IgE binding analyses of recombinant Der p 2 expressed from the hosts Escherichia coli and Pichia pastoris. 2010 Feb: 151(3) 190-8 https://www.ncbi.nlm.nih.gov/pubmed/19786799

381 Li Nan-Nan, Liu Ping, Chen Su-Juan, Lin Qing-Ping, Zhou Liang-Fan, Zhang Shuang-Quan Microbiological research. Construction and expression of a novel bioactive IFN-alpha2b/CM4 fusion protein in Escherichia coli. 2010 Feb: 165(2) 116-21 https://www.ncbi.nlm.nih.gov/pubmed/19246180

382 Wu Yi-Liang, Pan Li-Ping, Yu Si-Li, Li Hai-Hang Journal of biotechnology. Cloning, microbial expression and structure-activity relationship of polyphenol oxidases from Camellia sinensis. 2009 Dec: 145(1) 66-72 https://www.ncbi.nlm.nih.gov/pubmed/19857531

383 Schmidt Peter, Lindner Diana, Montag Cindy, Berndt Sandra, Beck-Sickinger Annette G, Rudolph Rainer, Huster Daniel Biotechnology progress. Prokaryotic expression, in vitro folding, and molecular pharmacological characterization of the neuropeptide Y receptor type 2. 2009 Dec: 25(6) 1732-9 https://www.ncbi.nlm.nih.gov/pubmed/19725122

384 Foster Toshana L, Tedbury Philip R, Pearson Arwen R, Harris Mark Biochimica et biophysica acta. A comparative analysis of the fluorescence properties of the wild-type and active site mutants of the hepatitis C virus autoprotease NS2-3. 2009 Nov: 1804(1) 212-22 https://www.ncbi.nlm.nih.gov/pubmed/19853065

385 Xu Zhe-rong, Yang Yun-mei, Gui Qi-feng, Zhang Li-na, Hu Lin Protein expression and purification. Expression, purification, and characterization of recombinant lumbrokinase PI239 in Escherichia coli. 2009 Nov: 69(2) 198-203 https://www.ncbi.nlm.nih.gov/pubmed/19716893

386 Morishige Tomohiro, Yoshioka Yasuo, Inakura Hiroshi, Tanabe Aya, Watanabe Hikaru, Yao Xinglei, Tsunoda Shin-Ichi, Tsutsumi Yasuo, Mukai Yohei, Okada Naoki, Nakagawa Shinsaku Immunology letters. LIGHT protein suppresses tumor growth by augmentation of immune response. 2009 Nov: 127(1) 33-8 https://www.ncbi.nlm.nih.gov/pubmed/19716382

387 Xiang Di, Zhang Jing, Chen Yizhe, Guo Yiping, Schalow Adrian, Zhang Zhonghui, Hu Xiaojia, Yu Hongjing, Zhao Mei, Zhu Shunying, Lu Huili, Wu Mingyuan, Yu Yan, Moldenhauer Anja, Han Wei Protein expression and purification. Expressions and purification of a mature form of recombinant human Chemerin in Escherichia coli. 2009 Nov: 69(2) 153-8 https://www.ncbi.nlm.nih.gov/pubmed/19647081

388 Tanabe Mikio, Iverson Tina M Acta crystallographica. Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB. 2009 Oct: Section F, Structural biology and crystallization communications. 65(Pt 10) 996-1000 https://www.ncbi.nlm.nih.gov/pubmed/19851005

389 Casaite Vida, Bruzyte Simona, Bukauskas Virginijus, Setkus Arunas, Morozova-Roche Ludmilla A, Meskys Rolandas Protein engineering, design & selection : PEDS. Expression and purification of active recombinant equine lysozyme in Escherichia coli. 2009 Oct: 22(11) 649-54 https://www.ncbi.nlm.nih.gov/pubmed/19651623

390 Miki Yuta, Morales María, Ruiz-Dueñas Francisco J, Martínez María Jesús, Wariishi Hiroyuki, Martínez Angel T Protein expression and purification. Escherichia coli expression and in vitro activation of a unique ligninolytic peroxidase that has a catalytic tyrosine residue. 2009 Oct: 68(2) 208-14 https://www.ncbi.nlm.nih.gov/pubmed/19505579

391 Bab-Dinitz Elizabeta, Albeck Shira, Peleg Yoav, Brumfeld Vlad, Gottschalk Kay E, Karlish Steven J D Biochemistry. A C-terminal lobe of the beta subunit of Na,K-ATPase and H,K-ATPase resembles cell adhesion molecules. 2009 Sep: 48(36) 8684-91 https://www.ncbi.nlm.nih.gov/pubmed/19694409

392 Sharabi Oz, Peleg Yoav, Mashiach Efrat, Vardy Eyal, Ashani Yacov, Silman Israel, Sussman Joel L, Shifman Julia M Protein engineering, design & selection : PEDS. Design, expression and characterization of mutants of fasciculin optimized for interaction with its target, acetylcholinesterase. 2009 Sep: 22(10) 641-8 https://www.ncbi.nlm.nih.gov/pubmed/19643977

393 Kawakami Ryushi, Sakuraba Haruhiko, Goda Shuichiro, Tsuge Hideaki, Ohshima Toshihisa Biochimica et biophysica acta. Refolding, characterization and crystal structure of (S)-malate dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix. 2009 Sep: 1794(10) 1496-504 https://www.ncbi.nlm.nih.gov/pubmed/19555779

394 Ersoy Maria, Matic Sandra, Schmidt Stefan R Biomedical chromatography : BMC. On-column refolding of corticotropin-releasing factor receptor 1 extracellular domain by size exclusion chromatography. 2009 Sep: 23(10) 1121-8 https://www.ncbi.nlm.nih.gov/pubmed/19404927

395 Stefan Alessandra, Ugolini Luisa, Martelli Elena, Palmieri Sandro, Hochkoeppler Alejandro Journal of bioscience and bioengineering. Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli. 2009 Aug: 108(4) 282-5 https://www.ncbi.nlm.nih.gov/pubmed/19716515

396 Tian Xiaohui, Wang Aiping, Qiao Songlin, Zhang Gaiping, Xi Jun, Li Xuewu, Liu Yunchao, Xu Yuqing Protein expression and purification. Expression, purification and characterization of a functional extracellular domain of porcine FcgammaRII. 2009 Aug: 68(1) 12-7 https://www.ncbi.nlm.nih.gov/pubmed/19576990

397 Singh Surinder M, Sharma Aparna, Panda Amulya K Protein expression and purification. High throughput purification of recombinant human growth hormone using radial flow chromatography. 2009 Aug: 68(1) 54-9 https://www.ncbi.nlm.nih.gov/pubmed/19500673

398 Khan Muhammad Altaf, Sadaf Saima, Sajjad Muhammad, Waheed Akhtar M Protein expression and purification. Production enhancement and refolding of caprine growth hormone expressed in Escherichia coli. 2009 Aug: 68(1) 85-9 https://www.ncbi.nlm.nih.gov/pubmed/19477280

399 O'Connell Anthony, Robin Gautier, Kobe Bostjan, Botella José Ramón Protein expression and purification. Biochemical characterization of Arabidopsis developmentally regulated G-proteins (DRGs). 2009 Jul: 67(2) 88-95 https://www.ncbi.nlm.nih.gov/pubmed/19460440

400 Fonseca Raquel Gomes, Ferreira Tatiana Lopes, Ward Richard J Protein expression and purification. Refolding and purification of the human secreted group IID phospholipase A2 expressed as inclusion bodies in Escherichia coli. 2009 Jul: 67(2) 82-7 https://www.ncbi.nlm.nih.gov/pubmed/19376232

401 Li Yanwen, Hu Xuchu, Liu Xiaoquan, Xu Jing, Hu Fengyu, Ma Changling, Yu Xinbing Parasitology research. Molecular cloning and analysis of stage and tissue-specific expression of Cathepsin L-like protease from Clonorchis sinensis. 2009 Jun: 105(2) 447-52 https://www.ncbi.nlm.nih.gov/pubmed/19308452

402 Liu Baoye, Lu Yan, Xin Zhiyong, Zhang Zengyan Biotechnology letters. Identification and antifungal assay of a wheat beta-1,3-glucanase. 2009 Jun: 31(7) 1005-10 https://www.ncbi.nlm.nih.gov/pubmed/19267236

403 Qian Xiaomin, Qi Jianxun, Chu Fuliang, Liu Jun, Li Qing, Yan Jinghua Acta crystallographica. Crystallization and preliminary X-ray analysis of the V domain of human nectin-2. 2009 May: Section F, Structural biology and crystallization communications. 65(Pt 6) 615-7 https://www.ncbi.nlm.nih.gov/pubmed/19478445

404 Chen Yu, Leong Susanna Su Jan Journal of chromatography. Adsorptive refolding of a highly disulfide-bonded inclusion body protein using anion-exchange chromatography. 2009 May: A. 1216(24) 4877-86 https://www.ncbi.nlm.nih.gov/pubmed/19419725

405 Whitney John C C, Neculai A Mirela, Ohman Dennis E, Howell P Lynne Acta crystallographica. Expression, refolding, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgE. 2009 May: Section F, Structural biology and crystallization communications. 65(Pt 5) 463-6 https://www.ncbi.nlm.nih.gov/pubmed/19407377

406 Liu Peng, Marzahn Melissa R, Robbins Arthur H, Gutiérrez-de-Terán Hugo, Rodríguez David, McClung Scott H, Stevens Stanley M, Yowell Charles A, Dame John B, McKenna Robert, Dunn Ben M Biochemistry. Recombinant plasmepsin 1 from the human malaria parasite plasmodium falciparum: enzymatic characterization, active site inhibitor design, and structural analysis. 2009 May: 48(19) 4086-99 https://www.ncbi.nlm.nih.gov/pubmed/19271776

407 Su Hongsheng, Jack Michael, McIntosh Laura M, Perdomo Liliana, Choy Bonny S F, Finck Barbara K, McDonald John R Protein expression and purification. Clinical grade production and characterization of a fusion protein comprised of the chemokine CCL2-ligand genetically fused to a mutated and truncated form of the Shiga A1 subunit. 2009 May: 66(2) 149-57 https://www.ncbi.nlm.nih.gov/pubmed/19264131

408 Walgraffe David, Mattéotti Christel, El Bakkoury Mohamed, Garcia Lida, Marchand Céline, Bullens Dominique, Vandenbranden Michel, Jacquet Alain The Journal of allergy and clinical immunology. A hypoallergenic variant of Der p 1 as a candidate for mite allergy vaccines. 2009 May: 123(5) 1150-6 https://www.ncbi.nlm.nih.gov/pubmed/19152967

409 Ahn Taeho, Yun Chul-Ho, Chae Ho Zoon, Kim Hyung-Ryong, Chae Han-Jung Protein expression and purification. Refolding and reconstitution of human recombinant Bax inhibitor-1 into liposomes from inclusion bodies expressed in Escherichia coli. 2009 Apr: 66(1) 35-8 https://www.ncbi.nlm.nih.gov/pubmed/19284981

410 Xu Jiang, Hu Yongliang, Gong Meiliang, Sun Chenjing, Xu Zhikai, Li Zhuyi, Wu Xingan Protein expression and purification. An improved method for refolding recombinant decay accelerating factor for therapeutic studies. 2009 Apr: 66(1) 102-6 https://www.ncbi.nlm.nih.gov/pubmed/19254764

411 Zelena Kateryna, Zorn Holger, Nimtz Manfred, Berger Ralf Günter Archives of microbiology. Heterologous expression of the msp2 gene from Marasmius scorodonius. 2009 Apr: 191(5) 397-402 https://www.ncbi.nlm.nih.gov/pubmed/19247632

412 Wang Dongming, Shi Linmei Applied biochemistry and biotechnology. High-level expression, purification, and in vitro refolding of soluble tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). 2009 Mar: 157(1) 1-9 https://www.ncbi.nlm.nih.gov/pubmed/19306078

413 Choudhury Debi, Roy Sumana, Chakrabarti Chandana, Biswas Sampa, Dattagupta J K Phytochemistry. Production and recovery of recombinant propapain with high yield. 2009 Mar: 70(4) 465-72 https://www.ncbi.nlm.nih.gov/pubmed/19272620

414 Wen Qian, Ma Li, Luo Wei, Zhou Ming-Qian, Wang Xiao-Ning Biomedical and environmental sciences : BES. Expression, purification, and refolding of recombinant fusion protein hIL-2/mGM-CSF. 2009 Mar: 21(6) 509-13 https://www.ncbi.nlm.nih.gov/pubmed/19263807

415 Verstraete Kenneth, Koch Sina, Ertugrul Sevgi, Vandenberghe Isabel, Aerts Maarten, Vandriessche Gonzalez, Thiede Christian, Savvides Savvas N The protein journal. Efficient production of bioactive recombinant human Flt3 ligand in E. coli. 2009 Mar: 28(2) 57-65 https://www.ncbi.nlm.nih.gov/pubmed/19184382

416 Sun Ziyong, Lu Wei, Jiang Aiqing, Chen Junyong, Tang Fengyuan, Liu Jian-Ning Protein expression and purification. Expression, purification and characterization of aprotinin and a human analogue of aprotinin. 2009 Mar: 65(2) 238-43 https://www.ncbi.nlm.nih.gov/pubmed/19152836

417 Yan Zier, Wang Huanchen, Cai Jiwen, Ke Hengming Protein expression and purification. Refolding and kinetic characterization of the phosphodiesterase-8A catalytic domain. 2009 Mar: 64(1) 82-8 https://www.ncbi.nlm.nih.gov/pubmed/18984055

418 Zhang Zhong-hui, Du Li-juan, Xiang Di, Zhu Shun-ying, Wu Ming-yuan, Lu Hui-li, Yu Yan, Han Wei Journal of Zhejiang University. Expression and purification of bioactive high-purity human midkine in Escherichia coli. 2009 Feb: Science. B. 10(2) 79-86 https://www.ncbi.nlm.nih.gov/pubmed/19235265

419 Togashi Hideaki, Nara Takayuki, Sekikawa Chisato, Kawakami Masayuki, Yaginuma Nakatsugu, Tsunoda Tatsuo, Sakaguchi Kengo, Mizukami Fujio Biotechnology progress. Refolding of lactate dehydrogenase by zeolite beta. 2009 Feb: 25(1) 200-6 https://www.ncbi.nlm.nih.gov/pubmed/19224588

420 Gustafsson Erika, Forsberg Cecilia, Haraldsson Karin, Lindman Stina, Ljung Lill, Furebring Christina Protein expression and purification. Purification of truncated and mutated Chemotaxis Inhibitory Protein of Staphylococcus aureus--an anti-inflammatory protein. 2009 Feb: 63(2) 95-101 https://www.ncbi.nlm.nih.gov/pubmed/18950716

421 Na B-K, Kang J-M, Cheun H-I, Cho S-H, Moon S-U, Kim T-S, Sohn W-M Parasitology. Cryptopain-1, a cysteine protease of Cryptosporidium parvum, does not require the pro-domain for folding. 2009 Jan: 136(2) 149-57 https://www.ncbi.nlm.nih.gov/pubmed/19091155

422 Lira C B B, Gui K E, Perez A M, da Silveira R C V, Gava L M, Ramos C H I, Cano M I N Biochimica et biophysica acta. DNA and heparin chaperone the refolding of purified recombinant replication protein A subunit 1 from Leishmania amazonensis. 2009 Jan: 1790(2) 119-25 https://www.ncbi.nlm.nih.gov/pubmed/19056467

423 Quintas-Granados Laura I, Orozco Esther, Brieba Luis G, Arroyo Rossana, Ortega-López Jaime Protein expression and purification. Purification, refolding and autoactivation of the recombinant cysteine proteinase EhCP112 from Entamoeba histolytica. 2009 Jan: 63(1) 26-32 https://www.ncbi.nlm.nih.gov/pubmed/18824105

424 Kovalskaya Natalia, Hammond Rosemarie W Protein expression and purification. Expression and functional characterization of the plant antimicrobial snakin-1 and defensin recombinant proteins. 2009 Jan: 63(1) 12-7 https://www.ncbi.nlm.nih.gov/pubmed/18824107

425 Nara Takayuki Y, Togashi Hideaki, Sekikawa Chisato, Kawakami Masayuki, Yaginuma Nakatsugu, Sakaguchi Kengo, Mizukami Fujio, Tsunoda Tatsuo Colloids and surfaces. Use of zeolite to refold a disulfide-bonded protein. 2009 Jan: B, Biointerfaces. 68(1) 68-73 https://www.ncbi.nlm.nih.gov/pubmed/18977122

426 Ciaccio Natalie A, Moreno Matthew L, Bauer Rachel L, Laurence Jennifer S Protein expression and purification. High-yield expression in E. coli and refolding of the bZIP domain of activating transcription factor 5. 2008 Dec: 62(2) 235-43 https://www.ncbi.nlm.nih.gov/pubmed/18718539

427 Rickert Keith W, Kelley Paul, Byrne Noel J, Diehl Ronald E, Hall Dawn L, Montalvo Allison M, Reid John C, Shipman Jennifer M, Thomas Bradley W, Munshi Sanjeev K, Darke Paul L, Su Hua-Poo The Journal of biological chemistry. Structure of human prostasin, a target for the regulation of hypertension. 2008 Dec: 283(50) 34864-72 https://www.ncbi.nlm.nih.gov/pubmed/18922802

428 Mark John, Li Xuguang, Cyr Terry, Fournier Sylvie, Jaentschke Bozena, Hefford Mary Alice Biochemical and biophysical research communications. SARS coronavirus: unusual lability of the nucleocapsid protein. 2008 Dec: 377(2) 429-33 https://www.ncbi.nlm.nih.gov/pubmed/18926799

429 Lu Diannan, Liu Zheng The journal of physical chemistry. Dynamic redox environment-intensified disulfide bond shuffling for protein refolding in vitro: molecular simulation and experimental validation. 2008 Nov: B. 112(47) 15127-33 https://www.ncbi.nlm.nih.gov/pubmed/18959394

430 Raghava Smita, Barua Bipasha, Singh Pradeep K, Das Mili, Madan Lalima, Bhattacharyya Sanchari, Bajaj Kanika, Gopal B, Varadarajan Raghavan, Gupta Munishwar N Protein science : a publication of the Protein Society. Refolding and simultaneous purification by three-phase partitioning of recombinant proteins from inclusion bodies. 2008 Nov: 17(11) 1987-97 https://www.ncbi.nlm.nih.gov/pubmed/18780821

431 Singh Pradeep K, Gupta Munishwar N Biochimica et biophysica acta. Simultaneous refolding and purification of a recombinant lipase with an intein tag by affinity precipitation with chitosan. 2008 Nov: 1784(11) 1825-9 https://www.ncbi.nlm.nih.gov/pubmed/18725331

432 Ohshima Yuji, Suzuki Yusuke, Nakatani Akihiro, Nohara Daisuke Journal of bioscience and bioengineering. Refolding of fully reduced bovine pancreatic trypsin. 2008 Oct: 106(4) 345-9 https://www.ncbi.nlm.nih.gov/pubmed/19000609

433 Ryu Kang, Kim Young-Hoon, Kim Younghwa, Lee Joon-Seok, Jeon Byeongwook, Kim Tae-Yoon Journal of microbiology and biotechnology. Increased yield of high-purity and active tetrameric recombinant human EC-SOD by solid phase refolding. 2008 Oct: 18(10) 1648-54 https://www.ncbi.nlm.nih.gov/pubmed/18955813

434 Curtis-Fisk Jaime, Spencer Ryan M, Weliky David P Protein expression and purification. Isotopically labeled expression in E. coli, purification, and refolding of the full ectodomain of the influenza virus membrane fusion protein. 2008 Oct: 61(2) 212-9 https://www.ncbi.nlm.nih.gov/pubmed/18640277

435 He Jintian, Wang Gaizhen, Xu Ruiguang, Feng Jinlin, Wang Jinlong, Su Huabo, Song Houyan Applied biochemistry and biotechnology. Refolding of a staphylokinase variant y1-Sak by reverse dilution. 2008 Oct: 151(1) 29-41 https://www.ncbi.nlm.nih.gov/pubmed/18785020

436 Rao D E C S, Rao K V, Reddy V D Journal of applied microbiology. Cloning and expression of Bacillus phytase gene (phy) in Escherichia coli and recovery of active enzyme from the inclusion bodies. 2008 Oct: 105(4) 1128-37 https://www.ncbi.nlm.nih.gov/pubmed/18479345

437 Borisova Vasilisa V, Frank Ludmila A, Markova Svetlana V, Burakova Ludmila P, Vysotski Eugene S Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology. Recombinant Metridia luciferase isoforms: expression, refolding and applicability for in vitro assay. 2008 Sep: 7(9) 1025-31 https://www.ncbi.nlm.nih.gov/pubmed/18754048

438 Khodarahmi Reza, Beyrami Mehdi, Soori Hosnieh Archives of biochemistry and biophysics. Appraisal of casein's inhibitory effects on aggregation accompanying carbonic anhydrase refolding and heat-induced ovalbumin fibrillogenesis. 2008 Sep: 477(1) 67-76 https://www.ncbi.nlm.nih.gov/pubmed/18485276

439 Hao Yanling, Huang Xinyi, Mei Xiaohong, Li Ruoyu, Zhai Zhengyuan, Yin Sheng, Huang Ying, Luo Yunbo Protein expression and purification. Expression, purification and characterization of pectin methylesterase inhibitor from kiwi fruit in Escherichia coli. 2008 Aug: 60(2) 221-4 https://www.ncbi.nlm.nih.gov/pubmed/18502664

440 Chura-Chambi Rosa Maria, Genova Luis Antonio, Affonso Regina, Morganti Ligia Analytical biochemistry. Refolding of endostatin from inclusion bodies using high hydrostatic pressure. 2008 Aug: 379(1) 32-9 https://www.ncbi.nlm.nih.gov/pubmed/18482572

441 Sharma Sandeep K, Goloubinoff Pierre, Christen Philipp Biochemical and biophysical research communications. Heavy metal ions are potent inhibitors of protein folding. 2008 Jul: 372(2) 341-5 https://www.ncbi.nlm.nih.gov/pubmed/18501191

442 Sharma K, Babu P V Cherish, Sasidhar P, Srinivas V K, Mohan V Krishna, Krishna Ella Protein expression and purification. Recombinant human epidermal growth factor inclusion body solubilization and refolding at large scale using expanded-bed adsorption chromatography from Escherichia coli. 2008 Jul: 60(1) 7-14 https://www.ncbi.nlm.nih.gov/pubmed/18430585

443 Chandrayan Sanjeev Kumar, Dhaunta Neeraj, Guptasarma Purnananda Protein expression and purification. Expression, purification, refolding and characterization of a putative lysophospholipase from Pyrococcus furiosus: retention of structure and lipase/esterase activity in the presence of water-miscible organic solvents at high temperatures. 2008 Jun: 59(2) 327-33 https://www.ncbi.nlm.nih.gov/pubmed/18400514

444 Zhang Qinghai, Horst Reto, Geralt Michael, Ma Xingquan, Hong Wen-Xu, Finn M G, Stevens Raymond C, Wüthrich Kurt Journal of the American Chemical Society. Microscale NMR screening of new detergents for membrane protein structural biology. 2008 Jun: 130(23) 7357-63 https://www.ncbi.nlm.nih.gov/pubmed/18479092

445 Shi Zhan-Xia, He Fei, Wang Li-Li, Liang Ying-Min, Han Hua, Wang Chao-Zhan, Zhao Qun, Geng Xin-Du Protein expression and purification. Expression, refolding, and purification of a truncated human Delta-like1, a ligand of Notch receptors. 2008 Jun: 59(2) 242-8 https://www.ncbi.nlm.nih.gov/pubmed/18367408

446 Dames Sonja A Protein expression and purification. A fast and simple method to prepare the FKBP-rapamycin binding domain of human target of rapamycin for NMR binding assays. 2008 May: 59(1) 31-7 https://www.ncbi.nlm.nih.gov/pubmed/18313330

447 Cowan Richard H, Davies Rick A, Pinheiro Teresa T J Analytical biochemistry. A screening system for the identification of refolding conditions for a model protein kinase, p38alpha. 2008 May: 376(1) 25-38 https://www.ncbi.nlm.nih.gov/pubmed/18294951

448 Jung Suhyun, Park Seongsoon Biotechnology letters. Improving the expression yield of Candida antarctica lipase B in Escherichia coli by mutagenesis. 2008 Apr: 30(4) 717-22 https://www.ncbi.nlm.nih.gov/pubmed/17985077

449 Legardinier Sébastien, Hubert Jean-François, Le Bihan Olivier, Tascon Christophe, Rocher Chantal, Raguénès-Nicol Céline, Bondon Arnaud, Hardy Serge, Le Rumeur Elisabeth Biochimica et biophysica acta. Sub-domains of the dystrophin rod domain display contrasting lipid-binding and stability properties. 2008 Apr: 1784(4) 672-82 https://www.ncbi.nlm.nih.gov/pubmed/18261477

450 Srivastava Atul K, Sharma Yogendra, Chary Kandala V R Protein expression and purification. Overexpression, on-column refolding and isotopic labeling of Hahellin from Hahella chejuensis, a putative member of the betagamma-crystallin superfamily. 2008 Apr: 58(2) 269-74 https://www.ncbi.nlm.nih.gov/pubmed/18267367

451 Fan Xiangdong, Xu Diansheng, Lu Bing, Xia Jie, Wei Dongzhi Journal of biochemical and biophysical methods. Improving the refolding of NTA protein by urea gradient and arginine gradient size-exclusion chromatography. 2008 Apr: 70(6) 1130-8 https://www.ncbi.nlm.nih.gov/pubmed/18241925

452 McCusker Emily, Robinson Anne Skaja Protein expression and purification. Refolding of G protein alpha subunits from inclusion bodies expressed in Escherichia coli. 2008 Apr: 58(2) 342-55 https://www.ncbi.nlm.nih.gov/pubmed/18249008

453 Okuda Hiroshi, Sakuhana Chihaya, Yamamoto Risa, Mizukami Yuko, Kawai Rika, Sumita Yusuke, Koga Motoki, Shirai Mutsunori, Matsuda Kazuhiko The Journal of biological chemistry. The intermediate domain defines broad nucleotide selectivity for protein folding in Chlamydophila GroEL1. 2008 Apr: 283(14) 9300-7 https://www.ncbi.nlm.nih.gov/pubmed/18230606

454 Ampapathi Ravi S, Creath Andrea L, Lou Dianne I, Craft John W, Blanke Steven R, Legge Glen B Journal of molecular biology. Order-disorder-order transitions mediate the activation of cholera toxin. 2008 Mar: 377(3) 748-60 https://www.ncbi.nlm.nih.gov/pubmed/18272180

455 Sun Ziyong, Xia Zhinan, Bi Feng, Liu Jian-Ning Applied microbiology and biotechnology. Expression and purification of human urodilatin by small ubiquitin-related modifier fusion in Escherichia coli. 2008 Mar: 78(3) 495-502 https://www.ncbi.nlm.nih.gov/pubmed/18189135

456 Yazdanparast Razieh, Khodagholi Fariba, Souri Effat International journal of biological macromolecules. Alkaline phosphatase refolding assisted by sequential use of oppositely charged detergents: a new artificial chaperone system. 2008 Mar: 42(2) 195-202 https://www.ncbi.nlm.nih.gov/pubmed/17980423

457 Shah Shweta, Gupta Munishwar N Biochimica et biophysica acta. Simultaneous refolding, purification and immobilization of xylanase with multi-walled carbon nanotubes. 2008 Feb: 1784(2) 363-7 https://www.ncbi.nlm.nih.gov/pubmed/18155676

458 Küster Frank, Seckler Robert Biochemistry. Pea seed lectin folds and oligomerizes via an intermediate not represented in the structural hierarchy. 2008 Feb: 47(8) 2458-65 https://www.ncbi.nlm.nih.gov/pubmed/18215068

459 Waldo Jennifer, Scherrer Michael PloS one. Production and initial characterization of Dad1p, a component of the Dam1-DASH kinetochore complex. 2008 : 3(12) e3888 https://www.ncbi.nlm.nih.gov/pubmed/19065263

460 Fernandez Pablo, Viebig Nicola K, Dechavanne Sébastien, Lépolard Catherine, Gysin Jürg, Scherf Artur, Gamain Benoit Malaria journal. Var2CSA DBL6-epsilon domain expressed in HEK293 induces limited cross-reactive and blocking antibodies to CSA binding parasites. 2008 : 7 170 https://www.ncbi.nlm.nih.gov/pubmed/18771584

461 Choi Seong Il, Han Kyoung Sim, Kim Chul Woo, Ryu Ki-Sun, Kim Byung Hee, Kim Kyun-Hwan, Kim Seo-Il, Kang Tae Hyun, Shin Hang-Cheol, Lim Keo-Heun, Kim Hyo Kyung, Hyun Jeong-Min, Seong Baik L PloS one. Protein solubility and folding enhancement by interaction with RNA. 2008 : 3(7) e2677 https://www.ncbi.nlm.nih.gov/pubmed/18628952

462 Zhang H, Yu M, Hu M, Qian L, Chen L, Shi M, Shen B, Guo N Preparative biochemistry & biotechnology. Overexpression, refolding, purification of Erbin PDZ domain from Escherichia coli and preparation of its polyclonal antibody. 2008 : 38(3) 282-93 https://www.ncbi.nlm.nih.gov/pubmed/18569875

463 Martínez Karla, de Anda Ramón, Hernández Georgina, Escalante Adelfo, Gosset Guillermo, Ramírez Octavio T, Bolívar Francisco G Microbial cell factories. Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system. 2008 : 7 1 https://www.ncbi.nlm.nih.gov/pubmed/18211716

464 Balan A, Ferreira R C C, Ferreira L C S Genetics and molecular research : GMR. Production of the refolded oligopeptide-binding protein (OppA) encoded by the citrus pathogen Xanthomonas axonopodis pv. Citri. 2008 : 7(1) 117-26 https://www.ncbi.nlm.nih.gov/pubmed/18273827

465 Tomasselli A G, Paddock D J, Emmons T L, Mildner A M, Leone J W, Lull J M, Cialdella J I, Prince D B, Fischer H D, Heinrikson R L, Benson T E Protein and peptide letters. High yield expression of human BACE constructs in Eschericia coli for refolding, purification, and high resolution diffracting crystal forms. 2008 : 15(2) 131-43 https://www.ncbi.nlm.nih.gov/pubmed/18289105

466 Boyle Denis M, Johnson Gary V, Heeren Robert A, Shell Robert E, Banerjee Amit, Gustafson Mark E Biotechnology and applied biochemistry. Evaluation of refolding conditions for a human recombinant fusion cytokine protein, promegapoietin-1a. 2008 Jan: 49(Pt 1) 73-83 https://www.ncbi.nlm.nih.gov/pubmed/17608622

467 Cherish Babu P V, Srinivas V K, Krishna Mohan V, Krishna Ella Journal of chromatography. Renaturation, purification and characterization of streptokinase expressed as inclusion body in recombinant E. coli. 2008 Jan: B, Analytical technologies in the biomedical and life sciences. 861(2) 218-26 https://www.ncbi.nlm.nih.gov/pubmed/17981102

468 Geng Shusheng, Chang Hong, Qin Weisong, Li Yan, Feng Jiannan, Shen Beifen Preparative biochemistry & biotechnology. Overexpression, effective renaturation, and bioactivity of novel single-chain antibodies against TNF-alpha. 2008 : 38(1) 74-86 https://www.ncbi.nlm.nih.gov/pubmed/18080912

469 Wisniewski Pawel, Master Adam, Kaminska Bozena Acta biochimica Polonica. Cloning and purification of functionally active Fas ligand interfering protein (FIP) expressed in Escherichia coli. 2008 : 55(1) 51-6 https://www.ncbi.nlm.nih.gov/pubmed/18204739

470 Huang Yuxiang, Choi Mei Yee, Au Shannon Wing Ngor, Au Deborah Man Yee, Lam Veronica Min Sien, Engel Paul C Molecular genetics and metabolism. Purification and detailed study of two clinically different human glucose 6-phosphate dehydrogenase variants, G6PD(Plymouth) and G6PD(Mahidol): Evidence for defective protein folding as the basis of disease. 2008 Jan: 93(1) 44-53 https://www.ncbi.nlm.nih.gov/pubmed/17959407

471 Bajorunaite Egle, Sereikaite Jolanta, Bumelis Vladas-Algirdas The protein journal. L-arginine suppresses aggregation of recombinant growth hormones in refolding process from E. coli inclusion bodies. 2007 Dec: 26(8) 547-55 https://www.ncbi.nlm.nih.gov/pubmed/17823856

472 Liu Yong-Dong, Zhang Gui-Feng, Li Jing-Jing, Chen Jing, Wang Yin-Jue, Ding Hong, Su Zhi-Guo Biotechnology and applied biochemistry. Identification of an oxidative refolding intermediate of recombinant consensus interferon from inclusion bodies and design of a two-stage strategy to promote correct disulfide-bond formation. 2007 Dec: 48(Pt 4) 189-98 https://www.ncbi.nlm.nih.gov/pubmed/17523923

473 Shu Zheng-Yu, Yan Yun-Jun, Yang Jiang-Ke, Xu Li Biotechnology letters. Aspergillus niger lipase: gene cloning, over-expression in Escherichia coli and in vitro refolding. 2007 Dec: 29(12) 1875-9 https://www.ncbi.nlm.nih.gov/pubmed/17636383

474 Okuda Hiroshi, Sakuhana Chihaya, Yamamoto Risa, Kawai Rika, Mizukami Yuko, Matsuda Kazuhiko Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. Effects of divalent cations on encapsulation and release in the GroEL-assisted folding. 2007 Dec: 20(6) 903-10 https://www.ncbi.nlm.nih.gov/pubmed/17242865

475 Wang Chaozhan, Wang Lili, Geng Xindu Biomedical chromatography : BMC. Renaturation with simultaneous purification of rhG-CSF from Escherichia coli by ion exchange chromatography. 2007 Dec: 21(12) 1291-6 https://www.ncbi.nlm.nih.gov/pubmed/17634960

476 Nageswara Rao P, Yadaiah Madasu, Roy Karnati R, Potu Harish, Bhuyan Abani K Protein expression and purification. Cloning, E. coli expression, refolding, and ATP-binding properties of a WD40-deleted Apaf-1 isoform. 2007 Dec: 56(2) 220-8 https://www.ncbi.nlm.nih.gov/pubmed/17931885

477 Tan Haidong, Wang Jinxia, Zhao Zongbao Kent Protein expression and purification. Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli. 2007 Nov: 56(1) 40-7 https://www.ncbi.nlm.nih.gov/pubmed/17703946

478 Fan Yanhua, Zhang Yongjun, Yang Xingyong, Pei Xiaoqiong, Guo Shujuan, Pei Yan Protein expression and purification. Expression of a Beauveria bassiana chitinase (Bbchit1) in Escherichia coli and Pichia pastoris. 2007 Nov: 56(1) 93-9 https://www.ncbi.nlm.nih.gov/pubmed/17692532

479 Schulenburg Cindy, Martinez-Senac Maria M, Löw Christian, Golbik Ralph, Ulbrich-Hofmann Renate, Arnold Ulrich The FEBS journal. Identification of three phases in Onconase refolding. 2007 Nov: 274(22) 5826-33 https://www.ncbi.nlm.nih.gov/pubmed/17944937

480 Seefeldt Matthew B, Crouch Chris, Kendrick Brent, Randolph Theodore W Biotechnology and bioengineering. Specific volume and adiabatic compressibility measurements of native and aggregated recombinant human interleukin-1 receptor antagonist: density differences enable pressure-modulated refolding. 2007 Oct: 98(2) 476-85 https://www.ncbi.nlm.nih.gov/pubmed/17335058

481 Tsybovsky Yaroslav, Shubenok Denis V, Kravchuk Zinaida I, Martsev Sergey P Protein engineering, design & selection : PEDS. Folding of an antibody variable domain in two functional conformations in vitro: calorimetric and spectroscopic study of the anti-ferritin antibody VL domain. 2007 Oct: 20(10) 481-90 https://www.ncbi.nlm.nih.gov/pubmed/17962224

482 Shimojo Kojiro, Oshima Tatsuya, Naganawa Hirochika, Goto Masahiro Biomacromolecules. Calixarene-assisted protein refolding via liquid-liquid extraction. 2007 Oct: 8(10) 3061-6 https://www.ncbi.nlm.nih.gov/pubmed/17718500

483 Lu Huili, Yu Mei, Sun Ye, Mao Wenwei, Wang Qun, Wu Mingyuan, Han Wei Protein expression and purification. Expression and purification of bioactive high-purity mouse monokine induced by IFN-gamma in Escherichia coli. 2007 Sep: 55(1) 132-8 https://www.ncbi.nlm.nih.gov/pubmed/17513126

484 Yin Fon-Yi, Chen Ya-Huei, Yu Chung-Ming, Pon Yu-Chin, Lee Hwei-Jen Biophysical journal. Kinetic refolding barrier of guanidinium chloride denatured goose delta-crystallin leads to regular aggregate formation. 2007 Aug: 93(4) 1235-45 https://www.ncbi.nlm.nih.gov/pubmed/17513375

485 Mannall Gareth J, Titchener-Hooker Nigel J, Dalby Paul A Biotechnology and bioengineering. Factors affecting protein refolding yields in a fed-batch and batch-refolding system. 2007 Aug: 97(6) 1523-34 https://www.ncbi.nlm.nih.gov/pubmed/17304557

486 Wang Steven S-S, Chang Che-Kuei, Liu Hwai-Shen Journal of chromatography. Effect of sample loop dimension on lysozyme refolding in size-exclusion chromatography. 2007 Aug: A. 1161(1-2) 56-63 https://www.ncbi.nlm.nih.gov/pubmed/17448483

487 Mark John K, Smith Sophie, Hefford Mary Alice Protein expression and purification. Over-expression and refolding of MAP kinase phosphatase 3. 2007 Aug: 54(2) 253-60 https://www.ncbi.nlm.nih.gov/pubmed/17459723

488 Al-Samarrai Taha H, Kirk Christopher A, Jones William T, Harvey Dawn, Sun Xiaolin Protein expression and purification. Expression in Escherichia coli and in vitro refolding of the plant transcription factor Arabidopsis thaliana RGL3. 2007 Jun: 53(2) 289-92 https://www.ncbi.nlm.nih.gov/pubmed/17317218

489 Rawat D S, Lumb V, Sharma Y D, Pasha S T, Singh G The Journal of communicable diseases. Histone as future drug target for malaria. 2007 Jun: 39(2) 119-28 https://www.ncbi.nlm.nih.gov/pubmed/18338692

490 Leong Susanna S J, Middelberg Anton P J Biotechnology and bioengineering. A simplified bioprocess for human alpha-fetoprotein production from inclusion bodies. 2007 May: 97(1) 99-117 https://www.ncbi.nlm.nih.gov/pubmed/17115449

491 Wang Xiqing, Lu Diannan, Austin Rebecca, Agarwal Arun, Mueller Leonard J, Liu Zheng, Wu Jianzhong, Feng Pingyun Langmuir : the ACS journal of surfaces and colloids. Protein refolding assisted by periodic mesoporous organosilicas. 2007 May: 23(10) 5735-9 https://www.ncbi.nlm.nih.gov/pubmed/17402759

492 Kwasek Anna, Osmark Peter, Allhorn Maria, Lindqvist Annika, Akerström Bo, Wasylewski Zygmunt Protein expression and purification. Production of recombinant human alpha1-microglobulin and mutant forms involved in chromophore formation. 2007 May: 53(1) 145-52 https://www.ncbi.nlm.nih.gov/pubmed/17169572

493 Veldkamp Christopher T, Peterson Francis C, Hayes Paulette L, Mattmiller Jessie E, Haugner John C, de la Cruz Norberto, Volkman Brian F Protein expression and purification. On-column refolding of recombinant chemokines for NMR studies and biological assays. 2007 Mar: 52(1) 202-9 https://www.ncbi.nlm.nih.gov/pubmed/17071104

494 Liu Yong-Dong, Li Jing-Jing, Wang Fang-wei, Chen Jing, Li Peng, Su Zhi-Guo Protein expression and purification. A newly proposed mechanism for arginine-assisted protein refolding--not inhibiting soluble oligomers although promoting a correct structure. 2007 Feb: 51(2) 235-42 https://www.ncbi.nlm.nih.gov/pubmed/16931044

495 Da-Hui Li, Gui-Liang Jian, Ying-Tao Zhang, Tie-Min Ai Applied microbiology and biotechnology. Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum. 2007 Feb: 74(1) 146-51 https://www.ncbi.nlm.nih.gov/pubmed/17024471

496 He Hua-Wei, Feng Shan, Pang Min, Zhou Hai-Meng, Yan Yong-Bin The international journal of biochemistry & cell biology. Role of the linker between the N- and C-terminal domains in the stability and folding of rabbit muscle creatine kinase. 2007 : 39(10) 1816-27 https://www.ncbi.nlm.nih.gov/pubmed/17616428

497 Yazdanparast Razieh, Esmaeili Mohammad Ali, Khodagholi Fariba International journal of biological macromolecules. Control of aggregation in protein refolding: cooperative effects of artificial chaperone and cold temperature. 2007 Jan: 40(2) 126-33 https://www.ncbi.nlm.nih.gov/pubmed/16875728

498 Swietnicki Wieslaw Current opinion in biotechnology. Folding aggregated proteins into functionally active forms. 2006 Aug: 17(4) 367-72 https://www.ncbi.nlm.nih.gov/pubmed/16740384

499 Díaz Susana, Pérez-Pomares Francisco, Pire Carmen, Ferrer Juan, Bonete María-José Extremophiles : life under extreme conditions. Gene cloning, heterologous overexpression and optimized refolding of the NAD-glutamate dehydrogenase from Haloferax mediterranei. 2006 Apr: 10(2) 105-15 https://www.ncbi.nlm.nih.gov/pubmed/16200391

500 Langenheim John F, Tan Dunyong, Walker Ameae M, Chen Wen Y Molecular endocrinology (Baltimore, Md. Two wrongs can make a right: dimers of prolactin and growth hormone receptor antagonists behave as agonists. 2006 Mar:). 20(3) 661-74 https://www.ncbi.nlm.nih.gov/pubmed/16269515

501 Lin Cheng-Wen, Lin Kuan-Hsun, Lyu Ping-Chiang, Chen Wei-June Virus research. Japanese encephalitis virus NS2B-NS3 protease binding to phage-displayed human brain proteins with the domain of trypsin inhibitor and basic region leucine zipper. 2006 Mar: 116(1-2) 106-13 https://www.ncbi.nlm.nih.gov/pubmed/16289409

502 Jin Ting, Guan Yi-Xin, Yao Shan-Jing, Lin Dong-Qiang, Cho Man-Gi Biotechnology and bioengineering. On-column refolding of recombinant human interferon-gamma inclusion bodies by expanded bed adsorption chromatography. 2006 Mar: 93(4) 755-60 https://www.ncbi.nlm.nih.gov/pubmed/16261630

503 Hossann Martin, Li Zhuoyu, Shi Yawei, Kreilinger Ulrike, Büttner Jörn, Vogel Pia D, Yuan Jingming, Wise John G, Trommer Wolfgang E Protein expression and purification. Novel immunotoxin: a fusion protein consisting of gelonin and an acetylcholine receptor fragment as a potential immunotherapeutic agent for the treatment of Myasthenia gravis. 2006 Mar: 46(1) 73-84 https://www.ncbi.nlm.nih.gov/pubmed/16230023

504 Fernández Marisa M, De Marzi Mauricio C, Berguer Paula, Burzyn Dalia, Langley Ries J, Piazzon Isabel, Mariuzza Roy A, Malchiodi Emilio L Molecular immunology. Binding of natural variants of staphylococcal superantigens SEG and SEI to TCR and MHC class II molecule. 2006 Mar: 43(7) 927-38 https://www.ncbi.nlm.nih.gov/pubmed/16023209

505 Geisbrecht Brian V, Bouyain Samuel, Pop Mihai Protein expression and purification. An optimized system for expression and purification of secreted bacterial proteins. 2006 Mar: 46(1) 23-32 https://www.ncbi.nlm.nih.gov/pubmed/16260150

506 Cho Uhn Soo, Bader Martin W, Amaya Maria F, Daley Margaret E, Klevit Rachel E, Miller Samuel I, Xu Wenqing Journal of molecular biology. Metal bridges between the PhoQ sensor domain and the membrane regulate transmembrane signaling. 2006 Mar: 356(5) 1193-206 https://www.ncbi.nlm.nih.gov/pubmed/16406409

507 Lu Haiqin, Zhu Jie, Zang Yuhui, Ze Yuguan, Qin Junchuan Protein expression and purification. Cloning, purification, and refolding of human paraoxonase-3 expressed in Escherichia coli and its characterization. 2006 Mar: 46(1) 92-9 https://www.ncbi.nlm.nih.gov/pubmed/16139510

508 Liu Mengyuan, Wang Xiangbin, Yin Changcheng, Zhang Zhong, Lin Qing, Zhen Yongsu, Huang Hualiang Biotechnology and applied biochemistry. One-step on-column purification and refolding of a single-chain variable fragment (scFv) antibody against tumour necrosis factor alpha. 2006 Mar: 43(Pt 3) 137-45 https://www.ncbi.nlm.nih.gov/pubmed/16293104

509 Singh Naresh, Sijwali Puran S, Pandey Kailash C, Rosenthal Philip J Experimental parasitology. Plasmodium falciparum: biochemical characterization of the cysteine protease falcipain-2'. 2006 Mar: 112(3) 187-92 https://www.ncbi.nlm.nih.gov/pubmed/16337629

510 Guan Yi-Xin, Fei Zheng-Zheng, Luo Man, Jin Ting, Yao Shan-Jing Journal of chromatography. Chromatographic refolding of recombinant human interferon gamma by an immobilized sht GroEL191-345 column. 2006 Feb: A. 1107(1-2) 192-7 https://www.ncbi.nlm.nih.gov/pubmed/16427063

511 El-Guindy Ayman S, Paek So Yeon, Countryman Jill, Miller George The Journal of biological chemistry. Identification of constitutive phosphorylation sites on the Epstein-Barr virus ZEBRA protein. 2006 Feb: 281(6) 3085-95 https://www.ncbi.nlm.nih.gov/pubmed/16321978

512 Gross Christian H, Abdul-Manan Norzehan, Fulghum John, Lippke Judith, Liu Xun, Prabhakar Prakash, Brennan Debra, Willis Melissa Swope, Faerman Carlos, Connelly Patrick, Raybuck Scott, Moore Jonathan The Journal of biological chemistry. Nucleotide-binding domains of cystic fibrosis transmembrane conductance regulator, an ABC transporter, catalyze adenylate kinase activity but not ATP hydrolysis. 2006 Feb: 281(7) 4058-68 https://www.ncbi.nlm.nih.gov/pubmed/16361259

513 Gutiérrez-González Luis H, Rojo-Domínguez Arturo, Cabrera-González Nallely E, Pérez-Montfort Ruy, Padilla-Zúñiga A Jaqueline Archives of biochemistry and biophysics. Loosely packed papain prosegment displays inhibitory activity. 2006 Feb: 446(2) 151-60 https://www.ncbi.nlm.nih.gov/pubmed/16427023

514 Chung Kyung Min, Nybakken Grant E, Thompson Bruce S, Engle Michael J, Marri Anantha, Fremont Daved H, Diamond Michael S Journal of virology. Antibodies against West Nile Virus nonstructural protein NS1 prevent lethal infection through Fc gamma receptor-dependent and -independent mechanisms. 2006 Feb: 80(3) 1340-51 https://www.ncbi.nlm.nih.gov/pubmed/16415011

515 Joucla Gilles, Pizzut Sandra, Monsan Pierre, Remaud-Simeon Magali FEBS letters. Construction of a fully active truncated alternansucrase partially deleted of its carboxy-terminal domain. 2006 Feb: 580(3) 763-8 https://www.ncbi.nlm.nih.gov/pubmed/16413550

516 Jenkins Huw T, Mark Linda, Ball Graeme, Persson Jenny, Lindahl Gunnar, Uhrin Dusan, Blom Anna M, Barlow Paul N The Journal of biological chemistry. Human C4b-binding protein, structural basis for interaction with streptococcal M protein, a major bacterial virulence factor. 2006 Feb: 281(6) 3690-7 https://www.ncbi.nlm.nih.gov/pubmed/16330538

517 Fang Min, Cheng Hong, Dai Zhongping, Bu Zimei, Sigal Luis J Virology. Immunization with a single extracellular enveloped virus protein produced in bacteria provides partial protection from a lethal orthopoxvirus infection in a natural host. 2006 Feb: 345(1) 231-43 https://www.ncbi.nlm.nih.gov/pubmed/16256161

518 Gasparian M E, Ostapchenko V G, Dolgikh D A, Kirpichnikov M P Biochemistry. Biochemical characterization of human enteropeptidase light chain. 2006 Feb: Biokhimii͡a. 71(2) 113-9 https://www.ncbi.nlm.nih.gov/pubmed/16489914

519 Byon Ju Yong, Ohira Tsuyoshi, Hirono Ikuo, Aoki Takashi Vaccine. Comparative immune responses in Japanese flounder, Paralichthys olivaceus after vaccination with viral hemorrhagic septicemia virus (VHSV) recombinant glycoprotein and DNA vaccine using a microarray analysis. 2006 Feb: 24(7) 921-30 https://www.ncbi.nlm.nih.gov/pubmed/16185791

520 Lee Seung-Hyun, Carpenter John F, Chang Byeong S, Randolph Theodore W, Kim Yong-Sung Protein science : a publication of the Protein Society. Effects of solutes on solubilization and refolding of proteins from inclusion bodies with high hydrostatic pressure. 2006 Feb: 15(2) 304-13 https://www.ncbi.nlm.nih.gov/pubmed/16385003

521 Sharma Sapna, Zhou Yu, Singh Bal Ram Protein expression and purification. Cloning, expression, and purification of C-terminal quarter of the heavy chain of botulinum neurotoxin type A. 2006 Feb: 45(2) 288-95 https://www.ncbi.nlm.nih.gov/pubmed/16143544

522 Cipáková Ingrid, Gasperík Juraj, Hostinová Eva Protein expression and purification. Expression and purification of human antimicrobial peptide, dermcidin, in Escherichia coli. 2006 Feb: 45(2) 269-74 https://www.ncbi.nlm.nih.gov/pubmed/16125410

523 Håkansson Pelle, Dahl Lina, Chilkova Olga, Domkin Vladimir, Thelander Lars The Journal of biological chemistry. The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding to the large Cdc22 subunit. 2006 Jan: 281(3) 1778-83 https://www.ncbi.nlm.nih.gov/pubmed/16317005

524 Jeevan A, McFarland C T, Yoshimura T, Skwor T, Cho H, Lasco T, McMurray D N Infection and immunity. Production and characterization of guinea pig recombinant gamma interferon and its effect on macrophage activation. 2006 Jan: 74(1) 213-24 https://www.ncbi.nlm.nih.gov/pubmed/16368975

525 Kothari Hema, Kumar Pranav, Singh Neeloo Protein expression and purification. Prokaryotic expression, purification, and polyclonal antibody production against a novel drug resistance gene of Leishmania donovani clinical isolate. 2006 Jan: 45(1) 15-21 https://www.ncbi.nlm.nih.gov/pubmed/16289919

526 Hoenig M, Caffall Z F, McGraw R A, Ferguson D C Domestic animal endocrinology. Cloning, expression and purification of feline proinsulin. 2006 Jan: 30(1) 28-37 https://www.ncbi.nlm.nih.gov/pubmed/15985360

527 Kang Kwan-Yub, Kim Sung-Geun, Kim Won-Kyung, You Hyun-Kyung, Kim Young-Jo, Lee Jong-Hyuk, Jung Kyung-Hwan, Kim Chan-Wha Protein expression and purification. Purification and characterization of a recombinant anti-angiogenic kringle fragment expressed in Escherichia coli: Purification and characterization of a tri-kringle fragment from human apolipoprotein (a) (kringle IV (9)-kringle IV (10)-kringle V). 2006 Jan: 45(1) 216-25 https://www.ncbi.nlm.nih.gov/pubmed/16260151

528 Leal Alexandre T, Pohl Paula C, Ferreira Carlos A S, Nascimento-Silva Maria C L, Sorgine Marcos H F, Logullo Carlos, Oliveira Pedro L, Farias Sandra E, da Silva Vaz Itabajara, Masuda Aoi Protein expression and purification. Purification and antigenicity of two recombinant forms of Boophilus microplus yolk pro-cathepsin expressed in inclusion bodies. 2006 Jan: 45(1) 107-14 https://www.ncbi.nlm.nih.gov/pubmed/16122942

529 Li Jing-Jing, Venkataramana Musturi, Sanyal Suparna, Janson Jan-Christer, Su Zhi-Guo Protein expression and purification. Immobilized beta-cyclodextrin polymer coupled to agarose gel properly refolding recombinant Staphylococcus aureus elongation factor-G in combination with detergent micelle. 2006 Jan: 45(1) 72-9 https://www.ncbi.nlm.nih.gov/pubmed/16006145

530 Gueguen Yannick, Herpin Amaury, Aumelas André, Garnier Julien, Fievet Julie, Escoubas Jean-Michel, Bulet Philippe, Gonzalez Marcelo, Lelong Christophe, Favrel Pascal, Bachère Evelyne The Journal of biological chemistry. Characterization of a defensin from the oyster Crassostrea gigas. Recombinant production, folding, solution structure, antimicrobial activities, and gene expression. 2006 Jan: 281(1) 313-23 https://www.ncbi.nlm.nih.gov/pubmed/16246846

531 Duan Weigang, Wang Shanzhi, Chen Min, Wang Cuifen, Zhang Luyong, Liu Jun, Sun Lixin, Yan Ming Biological & pharmaceutical bulletin. Harvest active recombinant Rho kinase from Escherichia coli. 2006 Jan: 29(1) 38-42 https://www.ncbi.nlm.nih.gov/pubmed/16394506

532 Barbosa Mônica Santiago, Báo Sônia Nair, Andreotti Patrícia Ferrari, de Faria Fabrícia P, Felipe Maria Sueli S, dos Santos Feitosa Luciano, Mendes-Giannini Maria José Soares, Soares Célia Maria de Almeida Infection and immunity. Glyceraldehyde-3-phosphate dehydrogenase of Paracoccidioides brasiliensis is a cell surface protein involved in fungal adhesion to extracellular matrix proteins and interaction with cells. 2006 Jan: 74(1) 382-9 https://www.ncbi.nlm.nih.gov/pubmed/16368993

533 Liu Xin, Clements Adrienne, Zhao Kehao, Marmorstein Ronen The Journal of biological chemistry. Structure of the human Papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor. 2006 Jan: 281(1) 578-86 https://www.ncbi.nlm.nih.gov/pubmed/16249186

534 Choi Nak-Won, Estes Mary K, Langridge William H R Journal of biotechnology. Mucosal immunization with a ricin toxin B subunit-rotavirus NSP4 fusion protein stimulates a Th1 lymphocyte response. 2006 Jan: 121(2) 272-83 https://www.ncbi.nlm.nih.gov/pubmed/16181698

535 Alonso Ana, Terrados Gloria, Picher Angel J, Giraldo Rafael, Blanco Luis, Larraga Vicente DNA repair. An intrinsic 5'-deoxyribose-5-phosphate lyase activity in DNA polymerase beta from Leishmania infantum supports a role in DNA repair. 2006 Jan: 5(1) 89-101 https://www.ncbi.nlm.nih.gov/pubmed/16174567

536 Chiku Hiroyuki, Kawai Akiko, Ishibashi Toyotaka, Takehara Masahide, Yanai Takuro, Mizukami Fujio, Sakaguchi Kengo Analytical biochemistry. A novel protein refolding method using a zeolite. 2006 Jan: 348(2) 307-14 https://www.ncbi.nlm.nih.gov/pubmed/16316618

537 Ostermann Nils, Eder Jörg, Eidhoff Ulf, Zink Florence, Hassiepen Ulrich, Worpenberg Susanne, Maibaum Jürgen, Simic Oliver, Hommel Ulrich, Gerhartz Bernd Journal of molecular biology. Crystal structure of human BACE2 in complex with a hydroxyethylamine transition-state inhibitor. 2006 Jan: 355(2) 249-61 https://www.ncbi.nlm.nih.gov/pubmed/16305800

538 Jin Hongjun, Hayes Garret L, Darbha Nithyanada S, Meyer Erik, LiWang Patricia J Biochemical and biophysical research communications. Investigation of CC and CXC chemokine quaternary state mutants. 2005 Dec: 338(2) 987-99 https://www.ncbi.nlm.nih.gov/pubmed/16256937

539 Fan Kexing, Zhou Qian, Wang Hao, Guo Yajun Hybridoma (2005). Preparation and characterization of a monoclonal antibody against the protein LIGHT. 2005 Dec: 24(6) 309-13 https://www.ncbi.nlm.nih.gov/pubmed/16332198

540 Babon Jeffrey J, Yao Shenggen, DeSouza David P, Harrison Christopher F, Fabri Louis J, Liepinsh Edvards, Scrofani Sergio D, Baca Manuel, Norton Raymond S The FEBS journal. Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain. 2005 Dec: 272(23) 6120-30 https://www.ncbi.nlm.nih.gov/pubmed/16302975

541 Akimoto Hidetoshi, Kinumi Tomoya, Ohmiya Yoshihiro Journal of biological rhythms. Circadian rhythm of a TCA cycle enzyme is apparently regulated at the translational level in the dinoflagellate Lingulodinium polyedrum. 2005 Dec: 20(6) 479-89 https://www.ncbi.nlm.nih.gov/pubmed/16275767

542 Clement Gilles, Boquet Didier, Mondoulet Lucie, Lamourette Patricia, Bernard Hervé, Wal Jean Michel Protein expression and purification. Expression in Escherichia coli and disulfide bridge mapping of PSC33, an allergenic 2S albumin from peanut. 2005 Dec: 44(2) 110-20 https://www.ncbi.nlm.nih.gov/pubmed/16023369

543 Compaan Deanne M, Gonzalez Lino C, Tom Irene, Loyet Kelly M, Eaton Dan, Hymowitz Sarah G The Journal of biological chemistry. Attenuating lymphocyte activity: the crystal structure of the BTLA-HVEM complex. 2005 Nov: 280(47) 39553-61 https://www.ncbi.nlm.nih.gov/pubmed/16169851

544 Folli Claudia, Viglione Simona, Busconi Marco, Berni Rodolfo Biochemical and biophysical research communications. Biochemical basis for retinol deficiency induced by the I41N and G75D mutations in human plasma retinol-binding protein. 2005 Nov: 336(4) 1017-22 https://www.ncbi.nlm.nih.gov/pubmed/16157297

545 Burgess Brandt R, Schuck Peter, Garboczi David N The Journal of biological chemistry. Dissection of merozoite surface protein 3, a representative of a family of Plasmodium falciparum surface proteins, reveals an oligomeric and highly elongated molecule. 2005 Nov: 280(44) 37236-45 https://www.ncbi.nlm.nih.gov/pubmed/16135515

546 Becker Lars, Bannwarth Michael, Meisinger Chris, Hill Kerstin, Model Kirstin, Krimmer Thomas, Casadio Rita, Truscott Kaye N, Schulz Georg E, Pfanner Nikolaus, Wagner Richard Journal of molecular biology. Preprotein translocase of the outer mitochondrial membrane: reconstituted Tom40 forms a characteristic TOM pore. 2005 Nov: 353(5) 1011-20 https://www.ncbi.nlm.nih.gov/pubmed/16213519

547 Kim Hye-Jung, Oh Deok-Kun Journal of biotechnology. Purification and characterization of an L-arabinose isomerase from an isolated strain of Geobacillus thermodenitrificans producing D-tagatose. 2005 Nov: 120(2) 162-73 https://www.ncbi.nlm.nih.gov/pubmed/16084621

548 Kim Myung Suk, Bae Sung-Hun, Yun Sang Hoon, Lee Hee Jung, Ji Sang Chun, Lee Ji Hyun, Srivastava Preeti, Lee Seol-Hoon, Chae Huiseok, Lee Younghoon, Choi Byong-Seok, Chattoraj Dhruba K, Lim Heon M Journal of bacteriology. Cnu, a novel oriC-binding protein of Escherichia coli. 2005 Oct: 187(20) 6998-7008 https://www.ncbi.nlm.nih.gov/pubmed/16199570

549 Klinke Sebastián, Zylberman Vanesa, Vega Daniel R, Guimarães Beatriz G, Braden Bradford C, Goldbaum Fernando A Journal of molecular biology. Crystallographic studies on decameric Brucella spp. Lumazine synthase: a novel quaternary arrangement evolved for a new function? 2005 Oct: 353(1) 124-37 https://www.ncbi.nlm.nih.gov/pubmed/16165152

550 Katoh Shizue, Murata Katsuyoshi, Kubota Yoshiki, Kumeta Hiroyuki, Ogura Kenji, Inagaki Fuyuhiko, Asayama Munehiko, Katoh Etsuko Protein expression and purification. Refolding and purification of recombinant OsNifU1A domain II that was expressed by Escherichia coli. 2005 Oct: 43(2) 149-56 https://www.ncbi.nlm.nih.gov/pubmed/15978837

551 Lange Christian, Patil Ganesh, Rudolph Rainer Protein science : a publication of the Protein Society. Ionic liquids as refolding additives: N'-alkyl and N'-(omega-hydroxyalkyl) N-methylimidazolium chlorides. 2005 Oct: 14(10) 2693-701 https://www.ncbi.nlm.nih.gov/pubmed/16195554

552 Chang Chung-ke, Sue Shih-Che, Yu Tsan-hung, Hsieh Chiu-Min, Tsai Cheng-Kun, Chiang Yen-Chieh, Lee Shin-jye, Hsiao Hsin-Hao, Wu Wen-Jin, Chang Chi-Fon, Huang Tai-huang FEBS letters. The dimer interface of the SARS coronavirus nucleocapsid protein adapts a porcine respiratory and reproductive syndrome virus-like structure. 2005 Oct: 579(25) 5663-8 https://www.ncbi.nlm.nih.gov/pubmed/16214138

553 Choi Nak-Won, Estes Mary K, Langridge William H R Vaccine. Oral immunization with a shiga toxin B subunit: rotavirus NSP4(90) fusion protein protects mice against gastroenteritis. 2005 Oct: 23(44) 5168-76 https://www.ncbi.nlm.nih.gov/pubmed/16040169

554 Holm Mai Marie, Naur Peter, Vestergaard Bente, Geballe Matthew T, Gajhede Michael, Kastrup Jette S, Traynelis Stephen F, Egebjerg Jan The Journal of biological chemistry. A binding site tyrosine shapes desensitization kinetics and agonist potency at GluR2. A mutagenic, kinetic, and crystallographic study. 2005 Oct: 280(42) 35469-76 https://www.ncbi.nlm.nih.gov/pubmed/16103115

555 Buonpane Rebecca A, Moza Beenu, Sundberg Eric J, Kranz David M Journal of molecular biology. Characterization of T cell receptors engineered for high affinity against toxic shock syndrome toxin-1. 2005 Oct: 353(2) 308-21 https://www.ncbi.nlm.nih.gov/pubmed/16171815

556 Bräsen Christopher, Schönheit Peter Extremophiles : life under extreme conditions. AMP-forming acetyl-CoA synthetase from the extremely halophilic archaeon Haloarcula marismortui: purification, identification and expression of the encoding gene, and phylogenetic affiliation. 2005 Oct: 9(5) 355-65 https://www.ncbi.nlm.nih.gov/pubmed/15947865

557 Ito Shinji, Murakami Akio, Sato Kyosuke, Nishina Yasuzo, Shiga Kiyoshi, Takahashi Tetsuo, Higashi Shoichi, Iseki Mineo, Watanabe Masakatsu Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology. Photocycle features of heterologously expressed and assembled eukaryotic flavin-binding BLUF domains of photoactivated adenylyl cyclase (PAC), a blue-light receptor in Euglena gracilis. 2005 Sep: 4(9) 762-9 https://www.ncbi.nlm.nih.gov/pubmed/16121289

558 Hosono Masahiro, Sugawara Shigeki, Ogawa Yukiko, Kohno Takayuki, Takayanagi Motoaki, Nitta Kazuo Biochimica et biophysica acta. Purification, characterization, cDNA cloning, and expression of asialofetuin-binding C-type lectin from eggs of shishamo smelt (Osmerus [Spirinchus] lanceolatus). 2005 Sep: 1725(2) 160-73 https://www.ncbi.nlm.nih.gov/pubmed/16112459

559 Inanami Osamu, Hashida Shukichi, Iizuka Daisuke, Horiuchi Motohiro, Hiraoka Wakako, Shimoyama Yuhei, Nakamura Hideo, Inagaki Fuyuhiko, Kuwabara Mikinori Biochemical and biophysical research communications. Conformational change in full-length mouse prion: a site-directed spin-labeling study. 2005 Sep: 335(3) 785-92 https://www.ncbi.nlm.nih.gov/pubmed/16095563

560 Easton Donna M, Smith Adam, Gallego Sara Gomez, Foxwell A Ruth, Cripps Allan W, Kyd Jennelle M Journal of bacteriology. Characterization of a novel porin protein from Moraxella catarrhalis and identification of an immunodominant surface loop. 2005 Sep: 187(18) 6528-35 https://www.ncbi.nlm.nih.gov/pubmed/16159786

561 Dutta Sheetij, Kaushal Deep C, Ware Lisa A, Puri Sunil K, Kaushal Nuzhat A, Narula Atul, Upadhyaya D S, Lanar David E Infection and immunity. Merozoite surface protein 1 of Plasmodium vivax induces a protective response against Plasmodium cynomolgi challenge in rhesus monkeys. 2005 Sep: 73(9) 5936-44 https://www.ncbi.nlm.nih.gov/pubmed/16113314

562 Dangerfield John A, Hohenadl Christine, Egerbacher Monika, Kodajova Petra, Salmons Brian, Günzburg Walter H Gene. HIV-1 Rev can specifically interact with MMTV RNA and upregulate gene expression. 2005 Sep: 358 17-30 https://www.ncbi.nlm.nih.gov/pubmed/16023306

563 Chen Ching-Ying, Rojanatavorn Kawan, Clark A Clay, Shih Jason C H Protein science : a publication of the Protein Society. Characterization and enzymatic degradation of Sup35NM, a yeast prion-like protein. 2005 Sep: 14(9) 2228-35 https://www.ncbi.nlm.nih.gov/pubmed/16081647

564 Sharma Aarti, Lambrechts Anja, Hao Le Thi, Le Thanh T, Sewry Caroline A, Ampe Christophe, Burghes Arthur H M, Morris Glenn E Experimental cell research. A role for complexes of survival of motor neurons (SMN) protein with gemins and profilin in neurite-like cytoplasmic extensions of cultured nerve cells. 2005 Sep: 309(1) 185-97 https://www.ncbi.nlm.nih.gov/pubmed/15975577

565 Boulant Steeve, Vanbelle Christophe, Ebel Christine, Penin François, Lavergne Jean-Pierre Journal of virology. Hepatitis C virus core protein is a dimeric alpha-helical protein exhibiting membrane protein features. 2005 Sep: 79(17) 11353-65 https://www.ncbi.nlm.nih.gov/pubmed/16103187

566 Bego Mariana, Maciejewski J, Khaiboullina S, Pari G, St Jeor S Journal of virology. Characterization of an antisense transcript spanning the UL81-82 locus of human cytomegalovirus. 2005 Sep: 79(17) 11022-34 https://www.ncbi.nlm.nih.gov/pubmed/16103153

567 Anisimov Roman, Brem Daniela, Heesemann Jürgen, Rakin Alexander FEMS microbiology letters. Molecular mechanism of YbtA-mediated transcriptional regulation of divergent overlapping promoters ybtA and irp6 of Yersinia enterocolitica. 2005 Sep: 250(1) 27-32 https://www.ncbi.nlm.nih.gov/pubmed/16019159

568 Aldridge Cassie, Møller Simon Geir The Journal of biological chemistry. The plastid division protein AtMinD1 is a Ca2+-ATPase stimulated by AtMinE1. 2005 Sep: 280(36) 31673-8 https://www.ncbi.nlm.nih.gov/pubmed/16014621

569 Sebollela Adriano, Cagliari Thiago C, Limaverde Gabriel S C S, Chapeaurouge Alex, Sorgine Marcos H F, Coelho-Sampaio Tatiana, Ramos Carlos H I, Ferreira Sérgio T The Journal of biological chemistry. Heparin-binding sites in granulocyte-macrophage colony-stimulating factor. Localization and regulation by histidine ionization. 2005 Sep: 280(36) 31949-56 https://www.ncbi.nlm.nih.gov/pubmed/16027123

570 Golding Hana, Khurana Surender, Yarovinsky Felix, King Lisa R, Abdoulaeva Galina, Antonsson Liselotte, Owman Christer, Platt Emily J, Kabat David, Andersen John F, Sher Alan The Journal of biological chemistry. CCR5 N-terminal region plays a critical role in HIV-1 inhibition by Toxoplasma gondii-derived cyclophilin-18. 2005 Aug: 280(33) 29570-7 https://www.ncbi.nlm.nih.gov/pubmed/15975927

571 Kim Kyu-Tae, Mok Myth T S, Edwards Michael R Biochemical and biophysical research communications. Protein kinase B from Giardia intestinalis. 2005 Aug: 334(2) 333-41 https://www.ncbi.nlm.nih.gov/pubmed/16018966

572 García-Orozco Karina D, López-Zavala Alonso A, Puentes-Camacho Daniel, Calderón-de-la-Barca Ana Maria, Sotelo-Mundo Rogerio R Biotechnology letters. Recombinant bacterial expression of the lysozyme from the tobacco-hornworm Manduca sexta with activity at low temperatures. 2005 Aug: 27(15) 1075-80 https://www.ncbi.nlm.nih.gov/pubmed/16132856

573 Derunes Céline, Briknarová Klára, Geng Liqing, Li Sheng, Gessner Chris R, Hewitt Krissi, Wu ShuangDing, Huang Shi, Woods Virgil I, Ely Kathryn R Biochemical and biophysical research communications. Characterization of the PR domain of RIZ1 histone methyltransferase. 2005 Aug: 333(3) 925-34 https://www.ncbi.nlm.nih.gov/pubmed/15964548

574 Kümmel Daniel, Müller Jürgen J, Roske Yvette, Misselwitz Rolf, Büssow Konrad, Heinemann Udo EMBO reports. The structure of the TRAPP subunit TPC6 suggests a model for a TRAPP subcomplex. 2005 Aug: 6(8) 787-93 https://www.ncbi.nlm.nih.gov/pubmed/16025134

575 Kamada Katsuhiko, Hanaoka Fumio Molecular cell. Conformational change in the catalytic site of the ribonuclease YoeB toxin by YefM antitoxin. 2005 Aug: 19(4) 497-509 https://www.ncbi.nlm.nih.gov/pubmed/16109374

576 Ferré Henrik, Ruffet Emmanuel, Nielsen Lise-Lotte B, Nissen Mogens Holst, Hobley Timothy J, Thomas Owen R T, Buus Søren Protein science : a publication of the Protein Society. A novel system for continuous protein refolding and on-line capture by expanded bed adsorption. 2005 Aug: 14(8) 2141-53 https://www.ncbi.nlm.nih.gov/pubmed/16046630

577 Liu Xipeng, Liu Jianhua Biotechnology letters. Signal peptide does not inhibit binding of biotin to streptavidin. 2005 Aug: 27(15) 1067-73 https://www.ncbi.nlm.nih.gov/pubmed/16132855

578 Schoner Brigitte E, Bramlett Kelli S, Guo Haihong, Burris Thomas P Molecular genetics and metabolism. Reconstitution of functional nuclear receptor proteins using high pressure refolding. 2005 Aug: 85(4) 318-22 https://www.ncbi.nlm.nih.gov/pubmed/15946878

579 Choi Yun-Jeong, Ryu Kyoung-Seok, Ko Yun-Mi, Chae Young-Kee, Pelton Jeffrey G, Wemmer David E, Choi Byong-Seok The Journal of biological chemistry. Biophysical characterization of the interaction domains and mapping of the contact residues in the XPF-ERCC1 complex. 2005 Aug: 280(31) 28644-52 https://www.ncbi.nlm.nih.gov/pubmed/15932882

580 Bryksin Anton V, Godfrey Henry P, Carbonaro Carol A, Wormser Gary P, Aguero-Rosenfeld Maria E, Cabello Felipe C Clinical and diagnostic laboratory immunology. Borrelia burgdorferi BmpA, BmpB, and BmpD proteins are expressed in human infection and contribute to P39 immunoblot reactivity in patients with Lyme disease. 2005 Aug: 12(8) 935-40 https://www.ncbi.nlm.nih.gov/pubmed/16085911

581 Basílico Guillermo, Roger Carolina Alvarez, Seigelchifer Mauricio, Kerner Nestor Journal of dermatological science. UV-specific DNA repair recombinant fusion enzyme: a new stable pharmacologically active principle suitable for photoprotection. 2005 Aug: 39(2) 81-8 https://www.ncbi.nlm.nih.gov/pubmed/16055003

582 Aizawa Youichi, Nogawa Nami, Kosaka Nobuyoshi, Maeda Yasutaka, Watanabe Takafumi, Miyazaki Hiroshi, Kato Takashi Journal of biochemistry. Expression of erythropoietin receptor-like molecule in Xenopus laevis and erythrocytopenia upon administration of its recombinant soluble form. 2005 Aug: 138(2) 167-75 https://www.ncbi.nlm.nih.gov/pubmed/16091591

583 Ai Jihui, Smith Paula, Wang Shuwei, Zhang Ping, Zheng X Long The Journal of biological chemistry. The proximal carboxyl-terminal domains of ADAMTS13 determine substrate specificity and are all required for cleavage of von Willebrand factor. 2005 Aug: 280(33) 29428-34 https://www.ncbi.nlm.nih.gov/pubmed/15975930

584 Giersing Birgitte, Miura Kazutoyo, Shimp Richard, Wang Jin, Zhou Hong, Orcutt Andrew, Stowers Anthony, Saul Allan, Miller Louis H, Long Carole, Singh Sanjay Infection and immunity. posttranslational modification of recombinant Plasmodium falciparum apical membrane antigen 1: impact on functional immune responses to a malaria vaccine candidate. 2005 Jul: 73(7) 3963-70 https://www.ncbi.nlm.nih.gov/pubmed/15972483

585 Fabbri Bradon J, Duff Stephen M G, Remsen Edward E, Chen Yun-Chia Sophia, Anderson John C, CaJacob Claire A Pest management science. The carboxyterminal processing protease of D1 protein: expression, purification and enzymology of the recombinant and native spinach proteins. 2005 Jul: 61(7) 682-90 https://www.ncbi.nlm.nih.gov/pubmed/15726567

586 Kodym Reinhard, Henöckl Christian, Fürweger Christoph Biochemical and biophysical research communications. Identification of the human DEAD-box protein p68 as a substrate of Tlk1. 2005 Jul: 333(2) 411-7 https://www.ncbi.nlm.nih.gov/pubmed/15950181

587 Junn Hyun Jung, Youn Jooho, Suh Kyong Hoon, Lee Sang Soo Protein expression and purification. Cloning and expression of Klebsiella phage K11 lysozyme gene. 2005 Jul: 42(1) 78-84 https://www.ncbi.nlm.nih.gov/pubmed/15882950

588 Kooi C, Corbett C R, Sokol P A Journal of bacteriology. Functional analysis of the Burkholderia cenocepacia ZmpA metalloprotease. 2005 Jul: 187(13) 4421-9 https://www.ncbi.nlm.nih.gov/pubmed/15968051

589 Le Moigne V, Robreau G, Borot C, Guesdon J-L, Mahana W Tuberculosis (Edinburgh, Scotland). Expression, immunochemical characterization and localization of the Mycobacterium tuberculosis protein p27. 2005 Jul: 85(4) 213-9 https://www.ncbi.nlm.nih.gov/pubmed/15958258

590 Feng Zhi-Ping, Keizer David W, Stevenson Rachel A, Yao Shenggen, Babon Jeffrey J, Murphy Vince J, Anders Robin F, Norton Raymond S Journal of molecular biology. Structure and inter-domain interactions of domain II from the blood-stage malarial protein, apical membrane antigen 1. 2005 Jul: 350(4) 641-56 https://www.ncbi.nlm.nih.gov/pubmed/15964019

591 Fogelgren Ben, Polgár Noémi, Szauter Kornélia Molnárné, Ujfaludi Zsuzsanna, Laczkó Rozália, Fong Keith S K, Csiszar Katalin The Journal of biological chemistry. Cellular fibronectin binds to lysyl oxidase with high affinity and is critical for its proteolytic activation. 2005 Jul: 280(26) 24690-7 https://www.ncbi.nlm.nih.gov/pubmed/15843371

592 Ding Yu, Jiang Ming, Jiang Weihua, Su Yang, Zhou Hanqing, Hu Xiaojian, Zhang Zhihong Protein expression and purification. Expression, purification, and characterization of recombinant human flotillin-1 in Escherichia coli. 2005 Jul: 42(1) 137-45 https://www.ncbi.nlm.nih.gov/pubmed/15939299

593 Crawford J L, Lun S, Demmer J, Eckery D C General and comparative endocrinology. Prolactin in the brushtail possum (Trichosurus vulpecula): development of homologous radioimmunoassay using recombinant possum prolactin. 2005 Jul: 142(3) 297-307 https://www.ncbi.nlm.nih.gov/pubmed/15935156

594 Yan Yutao, Liu Shuxian, Song Guangcheng, Xu Yixin, Dissous Colette Veterinary parasitology. Characterization of a novel vaccine candidate and serine proteinase inhibitor from Schistosoma japonicum (Sj serpin). 2005 Jul: 131(1-2) 53-60 https://www.ncbi.nlm.nih.gov/pubmed/15946799

595 He Xian-Hui, Xu Li-Hui, Liu Yi World journal of gastroenterology : WJG. Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes. 2005 Jul: 11(27) 4180-7 https://www.ncbi.nlm.nih.gov/pubmed/16015686

596 Hashiyama Kazuya, Takeuchi Ari, Makino Osamu Biochemical and biophysical research communications. Effects of single amino acid substitutions at the predicted coiled-coil or hydrophobic region on the self-assembly of phi29 replication protein, gp1. 2005 Jun: 331(4) 1310-6 https://www.ncbi.nlm.nih.gov/pubmed/15883018

597 Kim Bong-Jo, Mangala Selanere L, Hayashi Kiyoshi FEBS letters. Co-refolding of two peptide fragments derived from Agrobacterium tumefaciens beta-glucosidase with catalytic activity. 2005 Jun: 579(14) 3075-80 https://www.ncbi.nlm.nih.gov/pubmed/15907844

598 Karlsson Jan, Nilsson Thomas Protein expression and purification. The C subunit of Ideonella dechloratans chlorate reductase: expression, purification, refolding, and heme reconstitution. 2005 Jun: 41(2) 306-12 https://www.ncbi.nlm.nih.gov/pubmed/15866716

599 Fallahi Arzhang, Kroll Becky, Warner Lisa R, Oxford Rex J, Irwin Katey M, Mercer Linda M, Shadle Susan E, Oxford Julia Thom Protein science : a publication of the Protein Society. Structural model of the amino propeptide of collagen XI alpha1 chain with similarity to the LNS domains. 2005 Jun: 14(6) 1526-37 https://www.ncbi.nlm.nih.gov/pubmed/15930001

600 Shenoy A R, Srinivas A, Mahalingam M, Visweswariah S S Biochimie. An adenylyl cyclase pseudogene in Mycobacterium tuberculosis has a functional ortholog in Mycobacterium avium. 2005 Jun: 87(6) 557-63 https://www.ncbi.nlm.nih.gov/pubmed/15908099

601 Yang Xinxiu, Hu Weiguo, Li Feng, Xia Hengchuan, Zhang Zuchuan Protein expression and purification. Gene cloning, bacterial expression, in vitro refolding, and characterization of a single-chain Fv antibody against PreS1(21-47) fragment of HBsAg. 2005 Jun: 41(2) 341-8 https://www.ncbi.nlm.nih.gov/pubmed/15866720

602 Aslan Filiz M, Yu Yong, Mohr Scott C, Cantor Charles R Proceedings of the National Academy of Sciences of the United States of America. Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein. 2005 Jun: 102(24) 8507-12 https://www.ncbi.nlm.nih.gov/pubmed/15939877

603 Hijnen Marcel, van Gageldonk Pieter G M, Berbers Guy A M, van Woerkom Tiest, Mooi Frits R Protein expression and purification. The Bordetella pertussis virulence factor P.69 pertactin retains its immunological properties after overproduction in Escherichia coli. 2005 May: 41(1) 106-12 https://www.ncbi.nlm.nih.gov/pubmed/15802227

604 Hoell Ingunn A, Klemsdal Sonja S, Vaaje-Kolstad Gustav, Horn Svein J, Eijsink Vincent G H Biochimica et biophysica acta. Overexpression and characterization of a novel chitinase from Trichoderma atroviride strain P1. 2005 May: 1748(2) 180-90 https://www.ncbi.nlm.nih.gov/pubmed/15769595

605 Li Yirong, Ling Hongbo, Li Wenli, Tan Huarong Metabolic engineering. Improvement of nikkomycin production by enhanced copy of sanU and sanV in Streptomyces ansochromogenes and characterization of a novel glutamate mutase encoded by sanU and sanV. 2005 May: 7(3) 165-73 https://www.ncbi.nlm.nih.gov/pubmed/15885615

606 Ferreon Josephine C, Ferreon Allan Chris M, Li Kui, Lemon Stanley M The Journal of biological chemistry. Molecular determinants of TRIF proteolysis mediated by the hepatitis C virus NS3/4A protease. 2005 May: 280(21) 20483-92 https://www.ncbi.nlm.nih.gov/pubmed/15767257

607 Farnós Omar, Boué Oscar, Parra Francisco, Martín-Alonso José Manuel, Valdés Odaysa, Joglar Marisdania, Navea Leonor, Naranjo Paula, Lleonart Ricardo Journal of biotechnology. High-level expression and immunogenic properties of the recombinant rabbit hemorrhagic disease virus VP60 capsid protein obtained in Pichia pastoris. 2005 May: 117(3) 215-24 https://www.ncbi.nlm.nih.gov/pubmed/15862351

608 Gupta Aditi, Bai Tao, Murphy Vince, Strike Phillip, Anders Robin F, Batchelor Adrian H Protein expression and purification. Refolding, purification, and crystallization of apical membrane antigen 1 from Plasmodium falciparum. 2005 May: 41(1) 186-98 https://www.ncbi.nlm.nih.gov/pubmed/15802237

609 Linhart Birgit, Hartl Arnulf, Jahn-Schmid Beatrice, Verdino Petra, Keller Walter, Krauth Maria-Theresa, Valent Peter, Horak Friedrich, Wiedermann Ursula, Thalhamer Josef, Ebner Christof, Kraft Dietrich, Valenta Rudolf The Journal of allergy and clinical immunology. A hybrid molecule resembling the epitope spectrum of grass pollen for allergy vaccination. 2005 May: 115(5) 1010-6 https://www.ncbi.nlm.nih.gov/pubmed/15867859

610 Coulter-Mackie Marion B, Lian Qun, Wong Steve G Protein expression and purification. Overexpression of human alanine:glyoxylate aminotransferase in Escherichia coli: renaturation from guanidine-HCl and affinity for pyridoxal phosphate co-factor. 2005 May: 41(1) 18-26 https://www.ncbi.nlm.nih.gov/pubmed/15802217

611 Chen Hsiu-Hui, Vicente Cristina P, He Li, Tollefsen Douglas M, Wun Tze-Chein Blood. Fusion proteins comprising annexin V and Kunitz protease inhibitors are highly potent thrombogenic site-directed anticoagulants. 2005 May: 105(10) 3902-9 https://www.ncbi.nlm.nih.gov/pubmed/15677561

612 Cao Peng, Mei Jing Jing, Diao Zhen Yu, Zhang Shuang quan Protein expression and purification. Expression, refolding, and characterization of human soluble BAFF synthesized in Escherichia coli. 2005 May: 41(1) 199-206 https://www.ncbi.nlm.nih.gov/pubmed/15802238

613 Banères Jean-Louis, Mesnier Danielle, Martin Aimée, Joubert Lara, Dumuis Aline, Bockaert Joel The Journal of biological chemistry. Molecular characterization of a purified 5-HT4 receptor: a structural basis for drug efficacy. 2005 May: 280(21) 20253-60 https://www.ncbi.nlm.nih.gov/pubmed/15774473

614 Furutani Yutaka, Kato Akira, Fibriani Azzania, Hirata Taku, Kawai Ryoji, Jeon Ju-Hong, Fujii Yasuhisa, Kim In-Gyu, Kojima Soichi, Hirose Shigehisa The Journal of biological chemistry. Identification, evolution, and regulation of expression of Guinea pig trappin with an unusually long transglutaminase substrate domain. 2005 May: 280(21) 20204-15 https://www.ncbi.nlm.nih.gov/pubmed/15778505

615 Daniels Brodie B, Askew Sandra L, van de Venter Maryna, Oosthuizen Vaughan Cellular immunology. Production of biologically active recombinant human soluble CD23 and its effect on PBMCs isolated from hyper-IgE blood. 2005 Apr: 234(2) 146-53 https://www.ncbi.nlm.nih.gov/pubmed/16083870

616 Hillger Frank, Herr Gerhard, Rudolph Rainer, Schwarz Elisabeth The Journal of biological chemistry. Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor. 2005 Apr: 280(15) 14974-80 https://www.ncbi.nlm.nih.gov/pubmed/15695507

617 Karpushova A, Brümmer F, Barth S, Lange S, Schmid R D Applied microbiology and biotechnology. Cloning, recombinant expression and biochemical characterisation of novel esterases from Bacillus sp. associated with the marine sponge Aplysina aerophoba. 2005 Apr: 67(1) 59-69 https://www.ncbi.nlm.nih.gov/pubmed/15614567

618 Lambert Olivier, Benabdelhak Houssain, Chami Mohamed, Jouan Ludovic, Nouaille Emilie, Ducruix Arnaud, Brisson Alain Journal of structural biology. Trimeric structure of OprN and OprM efflux proteins from Pseudomonas aeruginosa, by 2D electron crystallography. 2005 Apr: 150(1) 50-7 https://www.ncbi.nlm.nih.gov/pubmed/15797729

619 Lee Chang-Hun, Jeong Sun-Young, Kim Beom-Jun, Choi Chang-Hoon, Kim Ji-Sun, Koo Byung-Mo, Seok Young-Jae, Yim Hyung-Soon, Kang Sa-Ouk Biochimica et biophysica acta. Dictyostelium CBP3 associates with actin cytoskeleton and is related to slug migration. 2005 Apr: 1743(3) 281-90 https://www.ncbi.nlm.nih.gov/pubmed/15843041

620 Li Jing-Jing, Venkataramana Musturi, Wang Ai-Qing, Sanyal Suparna, Janson Jan-Christer, Su Zhi-Guo Protein expression and purification. A mild hydrophobic interaction chromatography involving polyethylene glycol immobilized to agarose media refolding recombinant Staphylococcus aureus elongation factor G. 2005 Apr: 40(2) 327-35 https://www.ncbi.nlm.nih.gov/pubmed/15766874

621 Goda Shuichiro, Sakuraba Haruhiko, Kawarabayasi Yutaka, Ohshima Toshihisa Biochimica et biophysica acta. The first archaeal agmatinase from anaerobic hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization. 2005 Apr: 1748(1) 110-5 https://www.ncbi.nlm.nih.gov/pubmed/15752699

622 Yu Xiao-Qiang, Tracy Miles E, Ling Erjun, Scholz Frank R, Trenczek Tina Insect biochemistry and molecular biology. A novel C-type immulectin-3 from Manduca sexta is translocated from hemolymph into the cytoplasm of hemocytes. 2005 Apr: 35(4) 285-95 https://www.ncbi.nlm.nih.gov/pubmed/15763465

623 Langenhof Marc, Leong Susanna S J, Pattenden Leonard K, Middelberg Anton P J Journal of chromatography. Controlled oxidative protein refolding using an ion-exchange column. 2005 Apr: A. 1069(2) 195-201 https://www.ncbi.nlm.nih.gov/pubmed/15830945

624 Cabanne C, Noubhani A M, Hocquellet A, Dole F, Dieryck W, Santarelli X Journal of chromatography. Purification and on-column refolding of EGFP overexpressed as inclusion bodies in Escherichia coli with expanded bed anion exchange chromatography. 2005 Apr: B, Analytical technologies in the biomedical and life sciences. 818(1) 23-7 https://www.ncbi.nlm.nih.gov/pubmed/15722040

625 Bos Martine P, Tefsen Boris, Voet Pierre, Weynants Vincent, van Putten Jos P M, Tommassen Jan Infection and immunity. Function of neisserial outer membrane phospholipase a in autolysis and assessment of its vaccine potential. 2005 Apr: 73(4) 2222-31 https://www.ncbi.nlm.nih.gov/pubmed/15784566

626 Anisimov Roman, Brem Daniela, Heesemann Jürgen, Rakin Alexander International journal of medical microbiology : IJMM. Transcriptional regulation of high pathogenicity island iron uptake genes by YbtA. 2005 Apr: 295(1) 19-28 https://www.ncbi.nlm.nih.gov/pubmed/15861813

627 Lee Eun Kyung, Hwang Jin Ha, Shin Dong Yeon, Kim Dae Ihn, Yoo Yung Joon Protein expression and purification. Production of recombinant amyloid-beta peptide 42 as an ubiquitin extension. 2005 Mar: 40(1) 183-9 https://www.ncbi.nlm.nih.gov/pubmed/15721787

628 Dash Chandravanu, Sastry Murali, Rao Mala Biochemistry. Illustration of HIV-1 protease folding through a molten-globule-like intermediate using an experimental model that implicates alpha-crystallin and calcium ions. 2005 Mar: 44(10) 3725-34 https://www.ncbi.nlm.nih.gov/pubmed/15751949

629 Chen Zhonghai, Sun Xiaofen, Tang Kexuan Toxicon : official journal of the International Society on Toxinology. Cloning and expression of a novel cDNA encoding a mannose-binding lectin from Dendrobium officinale. 2005 Mar: 45(4) 535-40 https://www.ncbi.nlm.nih.gov/pubmed/15733576

630 Cheng Yun-Ching, Yan Fang-Jiun, Chang Long-Sen Biochimica et biophysica acta. Taiwan cobra chymotrypsin inhibitor: cloning, functional expression and gene organization. 2005 Mar: 1747(2) 213-20 https://www.ncbi.nlm.nih.gov/pubmed/15698956

631 Brecht Michael, Niemann Moritz, Schlüter Elke, Müller Ulrich F, Stuart Ken, Göringer H Ulrich Molecular cell. TbMP42, a protein component of the RNA editing complex in African trypanosomes, has endo-exoribonuclease activity. 2005 Mar: 17(5) 621-30 https://www.ncbi.nlm.nih.gov/pubmed/15749013

632 Broitman-Maduro Gina, Maduro Morris F, Rothman Joel H Developmental cell. The noncanonical binding site of the MED-1 GATA factor defines differentially regulated target genes in the C. elegans mesendoderm. 2005 Mar: 8(3) 427-33 https://www.ncbi.nlm.nih.gov/pubmed/15737937

633 Arora Sunil K, Pal Nirbhai S, Mujtaba S Experimental parasitology. Leishmania donovani: identification of novel vaccine candidates using human reactive sera and cell lines. 2005 Mar: 109(3) 163-70 https://www.ncbi.nlm.nih.gov/pubmed/15713447

634 Kumar P D, Krishnaswamy S Protein expression and purification. Overexpression, refolding, and purification of the major immunodominant outer membrane porin OmpC from Salmonella typhi: characterization of refolded OmpC. 2005 Mar: 40(1) 126-33 https://www.ncbi.nlm.nih.gov/pubmed/15721780

635 Gerlach Dieter, Schlott Bernhard, Zähringer Ulrich, Schmidt Karl-Hermann FEMS immunology and medical microbiology. N-acetyl-D-galactosamine/N-acetyl-D-glucosamine--recognizing lectin from the snail Cepaea hortensis: purification, chemical characterization, cloning and expression in E. coli. 2005 Feb: 43(2) 223-32 https://www.ncbi.nlm.nih.gov/pubmed/15681152

636 Kerman Aaron, Ananthanarayanan Vettai S Biochimica et biophysica acta. Expression and spectroscopic characterization of a large fragment of the mu-opioid receptor. 2005 Feb: 1747(1) 133-40 https://www.ncbi.nlm.nih.gov/pubmed/15680247

637 Joshi Bharat H, Puri Raj K Protein expression and purification. Optimization of expression and purification of two biologically active chimeric fusion proteins that consist of human interleukin-13 and Pseudomonas exotoxin in Escherichia coli. 2005 Feb: 39(2) 189-98 https://www.ncbi.nlm.nih.gov/pubmed/15642470

638 Colleluori Diana M, Tien Deborah, Kang Feirong, Pagliei Tara, Kuss Ryan, McCormick Timothy, Watson Karen, McFadden Karyn, Chaiken Irwin, Buckheit Robert W, Romano Joseph W Protein expression and purification. Expression, purification, and characterization of recombinant cyanovirin-N for vaginal anti-HIV microbicide development. 2005 Feb: 39(2) 229-36 https://www.ncbi.nlm.nih.gov/pubmed/15642474

639 Scholz Christian, Schaarschmidt Peter, Engel Alfred Michael, Andres Herbert, Schmitt Urban, Faatz Elke, Balbach Jochen, Schmid Franz Xaver Journal of molecular biology. Functional solubilization of aggregation-prone HIV envelope proteins by covalent fusion with chaperone modules. 2005 Feb: 345(5) 1229-41 https://www.ncbi.nlm.nih.gov/pubmed/15644217

640 Singh Vinay Kumar, Jia Zongchao Protein expression and purification. Refolding and one-step purification of recombinant human ARA70 over-expressed in Escherichia coli. 2005 Feb: 39(2) 283-7 https://www.ncbi.nlm.nih.gov/pubmed/15642480

641 Kim Kyu Rae, Kwon Dae Young, Yoon Suk Hoo, Kim Woo Yeon, Kim Kyung Hyun Protein expression and purification. Purification, refolding, and characterization of recombinant Pseudomonas fluorescens lipase. 2005 Jan: 39(1) 124-9 https://www.ncbi.nlm.nih.gov/pubmed/15596368

642 Konakova Marina, Pulst Stefan M Journal of molecular neuroscience : MN. Dystonia-associated forms of torsinA are deficient in ATPase activity. 2005 : 25(1) 105-17 https://www.ncbi.nlm.nih.gov/pubmed/15781971

643 Lloyd Janice A, McGrew Dharia A, Knight Kendall L Journal of molecular biology. Identification of residues important for DNA binding in the full-length human Rad52 protein. 2005 Jan: 345(2) 239-49 https://www.ncbi.nlm.nih.gov/pubmed/15571718

644 Lorentsen Rikke H, Fynbo Charlotte H, Thøgersen Hans C, Etzerodt Michael, Holtet Thor L Protein expression and purification. Expression, refolding, and purification of recombinant human granzyme B. 2005 Jan: 39(1) 18-26 https://www.ncbi.nlm.nih.gov/pubmed/15596356

645 Bi Xuezhi, Khush Gurdev S, Bennett John Plant & cell physiology. The rice nucellin gene ortholog OsAsp1 encodes an active aspartic protease without a plant-specific insert and is strongly expressed in early embryo. 2005 Jan: 46(1) 87-98 https://www.ncbi.nlm.nih.gov/pubmed/15659452

646 Weichsel Andrzej, Maes Estelle M, Andersen John F, Valenzuela Jesus G, Shokhireva Tatjana Kh, Walker F Ann, Montfort William R Proceedings of the National Academy of Sciences of the United States of America. Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein. 2005 Jan: 102(3) 594-9 https://www.ncbi.nlm.nih.gov/pubmed/15637157

647 Hou Wei-Hong, Wang Tian-Yun, Yuan Bao-Mei, Chai Yu-Rong, Jia Yan-Long, Tian Fang, Wang Jian-Min, Xue Le-Xun Acta biochimica et biophysica Sinica. Recombinant mouse canstatin inhibits chicken embryo chorioallantoic membrane angiogenesis and endothelial cell proliferation. 2004 Dec: 36(12) 845-50 https://www.ncbi.nlm.nih.gov/pubmed/15592653

648 Li Jing-Jing, Liu Yong-Dong, Wang Fang-Wei, Ma Guang-Hui, Su Zhi-Guo Journal of chromatography. Hydrophobic interaction chromatography correctly refolding proteins assisted by glycerol and urea gradients. 2004 Dec: A. 1061(2) 193-9 https://www.ncbi.nlm.nih.gov/pubmed/15641362

649 Yazdani Syed Shams, Shakri Ahmad Rushdi, Chitnis Chetan E Biotechnology letters. A high cell density fermentation strategy to produce recombinant malarial antigen in E. coli. 2004 Dec: 26(24) 1891-5 https://www.ncbi.nlm.nih.gov/pubmed/15672234

650 Acosta-Rivero Nelson, Rodriguez Armando, Musacchio Alexis, Falcón Viviana, Suarez Viana M, Martinez Gillian, Guerra Ivis, Paz-Lago Dalila, Morera Yanelys, de la Rosa María C, Morales-Grillo Juan, Dueñas-Carrera Santiago Biochemical and biophysical research communications. In vitro assembly into virus-like particles is an intrinsic quality of Pichia pastoris derived HCV core protein. 2004 Dec: 325(1) 68-74 https://www.ncbi.nlm.nih.gov/pubmed/15522201

651 Adams Jarrett J, Jang Christopher J, Spencer Holly L, Elliott Marc, Smith Steven P Protein expression and purification. Expression, purification and structural characterization of the scaffoldin hydrophilic X-module from the cellulosome of Clostridium thermocellum. 2004 Dec: 38(2) 258-63 https://www.ncbi.nlm.nih.gov/pubmed/15555941

652 Leung W H, Meng Z Q, Hui G, Ho W K K Biochimica et biophysica acta. Expression of an immunologically reactive merozoite surface protein (MSP-1(42)) in E. coli. 2004 Nov: 1675(1-3) 62-70 https://www.ncbi.nlm.nih.gov/pubmed/15535968

653 Ferry Gilles, Ubeaud Caroline, Dauly Claire, Mozo Julien, Guillard Sophie, Berger Sylvie, Jimenez Sandrine, Scoul Catherine, Leclerc Gregory, Yous Saïd, Delagrange Philippe, Boutin Jean A Protein expression and purification. Purification of the recombinant human serotonin N-acetyltransferase (EC 2.3.1.87): further characterization of and comparison with AANAT from other species. 2004 Nov: 38(1) 84-98 https://www.ncbi.nlm.nih.gov/pubmed/15477086

654 Wu Heng, Gao Jun, Sharif Wallace D, Davidson Mari K, Wahls Wayne P Protein expression and purification. Purification, folding, and characterization of Rec12 (Spo11) meiotic recombinase of fission yeast. 2004 Nov: 38(1) 136-44 https://www.ncbi.nlm.nih.gov/pubmed/15477092

655 Dahinden Pius, Pos Klaas M, Taralczak Malgorzata, Dimroth Peter Archives of microbiology. Oxaloacetate decarboxylase of Archaeoglobus fulgidus: cloning of genes and expression in Escherichia coli. 2004 Nov: 182(5) 414-20 https://www.ncbi.nlm.nih.gov/pubmed/15490124

656 Khandelwal Puneet, Choudhury Devapriya, Birah Ajanta, Reddy M K, Gupta Gorakh Prasad, Banerjee Nirupama Journal of bacteriology. Insecticidal pilin subunit from the insect pathogen Xenorhabdus nematophila. 2004 Oct: 186(19) 6465-76 https://www.ncbi.nlm.nih.gov/pubmed/15375127

657 Jang Seung-Hwan, Kang Dong-Ku, Chang Soo-Ik, Scheraga Harold A, Shin Hang-Cheol Biotechnology letters. High level production of bovine angiogenin in E. coli by an efficient refolding procedure. 2004 Oct: 26(19) 1501-4 https://www.ncbi.nlm.nih.gov/pubmed/15604787

658 Jiang Yaqin, Ye Jiang, Wu Haizhen, Zhang Huizhan Biotechnology letters. Cloning and expression of the polyhydroxyalkanote depolymerase gene from Pseudomonas putida, and characterization of the gene product. 2004 Oct: 26(20) 1585-8 https://www.ncbi.nlm.nih.gov/pubmed/15604801

659 Guo Chenyun, Li Zhuoyu, Shi Yawei, Xu Mingqun, Wise John G, Trommer Wolfgong E, Yuan Jingming Protein expression and purification. Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin. 2004 Oct: 37(2) 361-7 https://www.ncbi.nlm.nih.gov/pubmed/15358358

660 Fukada Haruhisa, Ozaki Yuichi, Pierce Andrew L, Adachi Shinji, Yamauchi Kohei, Hara Akihiko, Swanson Penny, Dickhoff Walton W General and comparative endocrinology. Salmon growth hormone receptor: molecular cloning, ligand specificity, and response to fasting. 2004 Oct: 139(1) 61-71 https://www.ncbi.nlm.nih.gov/pubmed/15474537

661 Dong Xiao-Yan, Huang Yan, Sun Yan Journal of biotechnology. Refolding kinetics of denatured-reduced lysozyme in the presence of folding aids. 2004 Oct: 114(1-2) 135-42 https://www.ncbi.nlm.nih.gov/pubmed/15464607

662 Seefeldt Matthew B, Ouyang Jun, Froland Wayne A, Carpenter John F, Randolph Theodore W Protein science : a publication of the Protein Society. High-pressure refolding of bikunin: efficacy and thermodynamics. 2004 Oct: 13(10) 2639-50 https://www.ncbi.nlm.nih.gov/pubmed/15388859

663 De Marzí Mauricio C, Fernández Marisa M, Sundberg Eric J, Molinero Luciana, Zwirner Norberto W, Llera Andrea S, Mariuzza Roy A, Malchiodi Emilio L European journal of biochemistry / FEBS. Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA. 2004 Oct: 271(20) 4075-83 https://www.ncbi.nlm.nih.gov/pubmed/15479236

664 Brizio Carmen, Brandsch Roderich, Bufano Daniela, Pochini Lorena, Indiveri Cesare, Barile Maria Protein expression and purification. Over-expression in Escherichia coli, functional characterization and refolding of rat dimethylglycine dehydrogenase. 2004 Oct: 37(2) 434-42 https://www.ncbi.nlm.nih.gov/pubmed/15358367

665 Canaan Stéphane, Maurin Damien, Chahinian Henri, Pouilly Bénédicte, Durousseau Cécile, Frassinetti Frédéric, Scappuccini-Calvo Loréna, Cambillau Christian, Bourne Yves European journal of biochemistry / FEBS. Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL family. 2004 Oct: 271(19) 3953-61 https://www.ncbi.nlm.nih.gov/pubmed/15373841

666 Betts Scott, Haase-Pettingell Cameron, Cook Kristen, King Jonathan Protein science : a publication of the Protein Society. Buried hydrophobic side-chains essential for the folding of the parallel beta-helix domains of the P22 tailspike. 2004 Sep: 13(9) 2291-303 https://www.ncbi.nlm.nih.gov/pubmed/15322277

667 Antolín Pilar, Girotti Alessandra, Arias Francisco Javier, Barriuso Begoña, Jiménez Pilar, Rojo Ma Angeles, Girbés Tomás Journal of biotechnology. Bacterial expression of biologically active recombinant musarmin 1 from bulbs of Muscari armeniacum L. and Miller. 2004 Sep: 112(3) 313-22 https://www.ncbi.nlm.nih.gov/pubmed/15313008

668 Geng Xindu, Bai Quan, Zhang Yangjun, Li Xiang, Wu Dan Journal of biotechnology. Refolding and purification of interferon-gamma in industry by hydrophobic interaction chromatography. 2004 Sep: 113(1-3) 137-49 https://www.ncbi.nlm.nih.gov/pubmed/15380653

669 White Jennifer, Lukacik Petra, Esser Dirk, Steward Michael, Giddings Naomi, Bright Jeremy R, Fritchley Sarah J, Morgan B Paul, Lea Susan M, Smith Geoffrey P, Smith Richard A G Protein science : a publication of the Protein Society. Biological activity, membrane-targeting modification, and crystallization of soluble human decay accelerating factor expressed in E. coli. 2004 Sep: 13(9) 2406-15 https://www.ncbi.nlm.nih.gov/pubmed/15322283

670 Hsieh Hui-Chu, Kumar Thallampuranam Krishnaswamy S, Yu Chin Biochemical and biophysical research communications. Cloning, overexpression, and characterization of cobrotoxin. 2004 Aug: 320(4) 1374-81 https://www.ncbi.nlm.nih.gov/pubmed/15303285

671 Lalitha P V, Ware Lisa A, Barbosa Arnoldo, Dutta Sheetij, Moch J Kathleen, Haynes J David, Fileta Bader B, White Charles E, Lanar David E Infection and immunity. Production of the subdomains of the Plasmodium falciparum apical membrane antigen 1 ectodomain and analysis of the immune response. 2004 Aug: 72(8) 4464-70 https://www.ncbi.nlm.nih.gov/pubmed/15271904

672 Choudhary Rakesh K, Pullakhandam Raghu, Ehtesham Nasreen Z, Hasnain Seyed E Protein expression and purification. Expression and characterization of Rv2430c, a novel immunodominant antigen of Mycobacterium tuberculosis. 2004 Aug: 36(2) 249-53 https://www.ncbi.nlm.nih.gov/pubmed/15249047

673 Standfuss Joerg, Kühlbrandt Werner The Journal of biological chemistry. The three isoforms of the light-harvesting complex II: spectroscopic features, trimer formation, and functional roles. 2004 Aug: 279(35) 36884-91 https://www.ncbi.nlm.nih.gov/pubmed/15208324

674 Vincent Patrick, Dieryck Wilfrid, Maneta-Peyret Lilly, Moreau Patrick, Cassagne Claude, Santarelli Xavier Journal of chromatography. Chromatographic purification of an insoluble histidine tag recombinant Ykt6p SNARE from Arabidopsis thaliana over-expressed in E. coli. 2004 Aug: B, Analytical technologies in the biomedical and life sciences. 808(1) 83-9 https://www.ncbi.nlm.nih.gov/pubmed/15236690

675 Callaghan Anastasia J, Aurikko Jukka P, Ilag Leopold L, Günter Grossmann J, Chandran Vidya, Kühnel Karin, Poljak Leonora, Carpousis Agamennon J, Robinson Carol V, Symmons Martyn F, Luisi Ben F Journal of molecular biology. Studies of the RNA degradosome-organizing domain of the Escherichia coli ribonuclease RNase E. 2004 Jul: 340(5) 965-79 https://www.ncbi.nlm.nih.gov/pubmed/15236960

676 Wang Chaozhan, Geng Xindu, Wang Dawei, Tian Bo Journal of chromatography. Purification of recombinant bovine normal prion protein PrP(104-242) by HPHIC. 2004 Jul: B, Analytical technologies in the biomedical and life sciences. 806(2) 185-90 https://www.ncbi.nlm.nih.gov/pubmed/15171928

677 Han Yu-Gang, Liu He-Li, Zheng Hong-Jin, Li Sheng-Guang, Bi Ru-Chang Protein expression and purification. Purification and refolding of human alpha5-subunit (PSMA5) of the 20S proteasome, expressed as inclusion bodies in Escherichia coli. 2004 Jun: 35(2) 360-5 https://www.ncbi.nlm.nih.gov/pubmed/15135414

678 Gebhard Leopoldo G, Carrizo Federico U, Stern Ana L, Burgardt Noelia I, Faivovich Julián, Lavilla Esteban, Ermácora Mario R European journal of biochemistry / FEBS. A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii. 2004 Jun: 271(11) 2117-26 https://www.ncbi.nlm.nih.gov/pubmed/15153102

679 Wright Edward, Serpersu Engin H Protein expression and purification. Isolation of aminoglycoside nucleotidyltransferase (2'')-Ia from inclusion bodies as active, monomeric enzyme. 2004 Jun: 35(2) 373-80 https://www.ncbi.nlm.nih.gov/pubmed/15135416

680 Asp Torben, Bowra Steve, Borg Søren, Holm Preben Bach Biochimica et biophysica acta. Molecular cloning, functional expression in Escherichia coli and enzymatic characterisation of a cysteine protease from white clover (Trifolium repens). 2004 Jun: 1699(1-2) 111-22 https://www.ncbi.nlm.nih.gov/pubmed/15158718

681 Harrison Mark D, Dennison Christopher Proteins. Characterization of Arabidopsis thaliana stellacyanin: a comparison with umecyanin. 2004 May: 55(2) 426-35 https://www.ncbi.nlm.nih.gov/pubmed/15048833

682 Yuan Ching, Reuland Janice M, Lee Lyndon, Huang Andrew J W Protein expression and purification. Optimized expression and refolding of human keratoepithelin in BL21 (DE3). 2004 May: 35(1) 39-45 https://www.ncbi.nlm.nih.gov/pubmed/15039064

683 Neely Alan, Garcia-Olivares Jennie, Voswinkel Stephan, Horstkott Hannelore, Hidalgo Patricia The Journal of biological chemistry. Folding of active calcium channel beta(1b) -subunit by size-exclusion chromatography and its role on channel function. 2004 May: 279(21) 21689-94 https://www.ncbi.nlm.nih.gov/pubmed/15016803

684 Jin Hyung-Joo, Dunn Michael A, Borthakur Dulal, Kim Yong Soo Protein expression and purification. Refolding and purification of unprocessed porcine myostatin expressed in Escherichia coli. 2004 May: 35(1) 1-10 https://www.ncbi.nlm.nih.gov/pubmed/15039059

685 Das D, Kriangkum J, Nagata L P, Fulton R E, Suresh M R Journal of virological methods. Development of a biotin mimic tagged ScFv antibody against western equine encephalitis virus: bacterial expression and refolding. 2004 May: 117(2) 169-77 https://www.ncbi.nlm.nih.gov/pubmed/15041214

686 Farina Anthony, Faiola Francesco, Martinez Ernest Protein expression and purification. Reconstitution of an E box-binding Myc:Max complex with recombinant full-length proteins expressed in Escherichia coli. 2004 Apr: 34(2) 215-22 https://www.ncbi.nlm.nih.gov/pubmed/15003254

687 Liñares David, Echevarria Iñigo, Mañá Paula Protein expression and purification. Single-step purification and refolding of recombinant mouse and human myelin oligodendrocyte glycoprotein and induction of EAE in mice. 2004 Apr: 34(2) 249-56 https://www.ncbi.nlm.nih.gov/pubmed/15003258

688 Cheng Yuan, Patel Dinshaw J Biochemical and biophysical research communications. An efficient system for small protein expression and refolding. 2004 Apr: 317(2) 401-5 https://www.ncbi.nlm.nih.gov/pubmed/15063772

689 Hung Kuo-Wei, Kumar T K S, Chi Ya-Hui, Chiu Ing-Ming, Yu Chin Biochemical and biophysical research communications. Molecular cloning, overexpression, and characterization of the ligand-binding D2 domain of fibroblast growth factor receptor. 2004 Apr: 317(1) 253-8 https://www.ncbi.nlm.nih.gov/pubmed/15047176

690 Chae Young Kee, Im Hana, Zhao Qin, Doelling Jed H, Vierstra Richard D, Markley John L Protein expression and purification. Prevention of aggregation after refolding by balanced stabilization-destabilization: production of the Arabidopsis thaliana protein APG8a (At4g21980) for NMR structure determination. 2004 Apr: 34(2) 280-3 https://www.ncbi.nlm.nih.gov/pubmed/15003262

691 Hohenblum Hubertus, Vorauer-Uhl Karola, Katinger Hermann, Mattanovich Diethard Journal of biotechnology. Bacterial expression and refolding of human trypsinogen. 2004 Apr: 109(1-2) 3-11 https://www.ncbi.nlm.nih.gov/pubmed/15063609

692 Stromer Thusnelda, Fischer Elke, Richter Klaus, Haslbeck Martin, Buchner Johannes The Journal of biological chemistry. Analysis of the regulation of the molecular chaperone Hsp26 by temperature-induced dissociation: the N-terminal domail is important for oligomer assembly and the binding of unfolding proteins. 2004 Mar: 279(12) 11222-8 https://www.ncbi.nlm.nih.gov/pubmed/14722093

693 Peterson Francis C, Elgin E Sonay, Nelson Timothy J, Zhang Fuming, Hoeger Theresa J, Linhardt Robert J, Volkman Brian F The Journal of biological chemistry. Identification and characterization of a glycosaminoglycan recognition element of the C chemokine lymphotactin. 2004 Mar: 279(13) 12598-604 https://www.ncbi.nlm.nih.gov/pubmed/14707146

694 Yan Hong-Li, Wang Wei-Ting, He Yan, Zhao Zhuan-You, Gao Yuan-Jian, Zhang Yi, Sun Shu-Han Acta biochimica et biophysica Sinica. Construction, expression, and characterization of a recombinant annexin B1-low molecular weight urokinase chimera in Escherichia coli. 2004 Mar: 36(3) 184-90 https://www.ncbi.nlm.nih.gov/pubmed/15202502

695 Li Da-Wei, Yu Jian-Feng, Chen Yong-Jing, Ma Hong-Bing, Wang Zheng-Fei, Zhu Yi-Bei, Zhang Xue-Gang Acta biochimica et biophysica Sinica. Refolding and characterization of recombinant human GST-PD-1 fusion protein expressed in Escherichia coli. 2004 Feb: 36(2) 141-6 https://www.ncbi.nlm.nih.gov/pubmed/14970911

696 Yang Xiao Ang, Dong Xue Yuan, Li Yan, Wang Yue Dan, Chen Wei Feng Protein expression and purification. Purification and refolding of a novel cancer/testis antigen BJ-HCC-2 expressed in the inclusion bodies of Escherichia coli. 2004 Feb: 33(2) 332-8 https://www.ncbi.nlm.nih.gov/pubmed/14711522

697 Jiang Haobo, Ma Congcong, Lu Zhi-Qiang, Kanost Michael R Insect biochemistry and molecular biology. Beta-1,3-glucan recognition protein-2 (betaGRP-2)from Manduca sexta; an acute-phase protein that binds beta-1,3-glucan and lipoteichoic acid to aggregate fungi and bacteria and stimulate prophenoloxidase activation. 2004 Jan: 34(1) 89-100 https://www.ncbi.nlm.nih.gov/pubmed/14976985

698 Hatakeyama Tomomitsu, Shiba Kouhei, Matsuo Noriaki, Fujimoto Tokiko, Oda Tatsuya, Sugawara Hajime, Aoyagi Haruhiko Journal of biochemistry. Characterization of recombinant CEL-I, a GalNAc-specific C-type lectin, expressed in Escherichia coli using an artificial synthetic gene. 2004 Jan: 135(1) 101-7 https://www.ncbi.nlm.nih.gov/pubmed/14999015

699 Raghu Pullakhandam, Ghosh Sudip, Soundarya Kanukolanu, Haseeb Abdul, Aruna Battu, Ehtesham Nasreen Z Biochemical and biophysical research communications. Dimerization of human recombinant resistin involves covalent and noncovalent interactions. 2004 Jan: 313(3) 642-6 https://www.ncbi.nlm.nih.gov/pubmed/14697240

700 Breitinger Ulrike, Breitinger Hans-Georg, Bauer Finn, Fahmy Karim, Glockenhammer Daniela, Becker Cord-Michael The Journal of biological chemistry. Conserved high affinity ligand binding and membrane association in the native and refolded extracellular domain of the human glycine receptor alpha1-subunit. 2004 Jan: 279(3) 1627-36 https://www.ncbi.nlm.nih.gov/pubmed/14593111

701 Boué O, Farnós O, González A, Fernández R, Acosta J A, Valdés R, González L J, Guanche Y, Izquierdo G, Suárez M, Domínguez I, Machado H, Rodríguez M, Lleonart R Experimental & applied acarology. Production and biochemical characterization of the recombinant Boophilus microplus Bm95 antigen from Pichia pastoris. 2004 : 32(1-2) 119-28 https://www.ncbi.nlm.nih.gov/pubmed/15139278

702 Gong Bolin, Wang Lili, Wang Chaozhan, Geng Xindu Journal of chromatography. Preparation of hydrophobic interaction chromatographic packings based on monodisperse poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads and their application. 2004 Jan: A. 1022(1-2) 33-9 https://www.ncbi.nlm.nih.gov/pubmed/14753769

703 Jaiswal Smita, Khanna Navin, Swaminathan Sathyamangalam Protein expression and purification. High-level expression and one-step purification of recombinant dengue virus type 2 envelope domain III protein in Escherichia coli. 2004 Jan: 33(1) 80-91 https://www.ncbi.nlm.nih.gov/pubmed/14680965

704 Hu Shao-Yang, Wu Jen-Leih, Huang Jan-Hsiung Journal of biotechnology. Production of tilapia insulin-like growth factor-2 in high cell density cultures of recombinant Escherichia coli. 2004 Jan: 107(2) 161-71 https://www.ncbi.nlm.nih.gov/pubmed/14711499

705 Fraser Deborah A, Harris Claire L, Williams Anwen S, Mizuno Masashi, Gallagher Sean, Smith Richard A G, Morgan B Paul The Journal of biological chemistry. Generation of a recombinant, membrane-targeted form of the complement regulator CD59: activity in vitro and in vivo. 2003 Dec: 278(49) 48921-7 https://www.ncbi.nlm.nih.gov/pubmed/14519760

706 Shen Ya-Ling, Xia Xiao-Xia, Zhang Yue, Liu Jian-Wen, Wei Dong-Zhi, Yang Sheng-Li Biotechnology letters. Refolding and purification of Apo2l/TRAIL produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli. 2003 Dec: 25(24) 2097-101 https://www.ncbi.nlm.nih.gov/pubmed/14969416

707 Couceyro Pastor R, Fritz Tammi Protein expression and purification. Production of recombinant CART peptides in Escherichia coli with agonist and antagonist effects on food intake in rats. 2003 Dec: 32(2) 185-93 https://www.ncbi.nlm.nih.gov/pubmed/14965763

708 Hodder Anthony N, Drew Damien R, Epa V Chandana, Delorenzi Mauro, Bourgon Richard, Miller Susanne K, Moritz Robert L, Frecklington David F, Simpson Richard J, Speed Terence P, Pike Robert N, Crabb Brendan S The Journal of biological chemistry. Enzymic, phylogenetic, and structural characterization of the unusual papain-like protease domain of Plasmodium falciparum SERA5. 2003 Nov: 278(48) 48169-77 https://www.ncbi.nlm.nih.gov/pubmed/13679369

709 Ananthanarayanan Bharath, Stahelin Robert V, Digman Michelle A, Cho Wonhwa The Journal of biological chemistry. Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains. 2003 Nov: 278(47) 46886-94 https://www.ncbi.nlm.nih.gov/pubmed/12954613

710 Xie Qiuhong, Matsunaga Shigeru, Shi Xiaohua, Ogawa Setsuko, Niimi Setsuko, Wen Zhesheng, Tokuyasu Ken, Machida Sachiko Protein expression and purification. Refolding and characterization of the functional ligand-binding domain of human lectin-like oxidized LDL receptor. 2003 Nov: 32(1) 68-74 https://www.ncbi.nlm.nih.gov/pubmed/14680941

711 Hushpulian D M, Savitski P A, Rojkova A M, Chubar T A, Fechina V A, Sakharov I Yu, Lagrimini L M, Tishkov V I, Gazaryan I G Biochemistry. Expression and refolding of tobacco anionic peroxidase from E. coli inclusion bodies. 2003 Nov: Biokhimii͡a. 68(11) 1189-94 https://www.ncbi.nlm.nih.gov/pubmed/14640960

712 Jung Sang Taek, Kim Moon Suk, Seo Ji Yeon, Kim Hyung Chul, Kim Youngho Protein expression and purification. Purification of enzymatically active human lysyl oxidase and lysyl oxidase-like protein from Escherichia coli inclusion bodies. 2003 Oct: 31(2) 240-6 https://www.ncbi.nlm.nih.gov/pubmed/14550642

713 Ivanenkov Vasily V, Murphy-Piedmonte Deirdre M, Kirley Terence L Biochemistry. Bacterial expression, characterization, and disulfide bond determination of soluble human NTPDase6 (CD39L2) nucleotidase: implications for structure and function. 2003 Oct: 42(40) 11726-35 https://www.ncbi.nlm.nih.gov/pubmed/14529283

714 Li Ming, Poliakov Anton, Danielson U Helena, Su Zhiguo, Janson Jan-Christer Biotechnology letters. Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures. 2003 Oct: 25(20) 1729-34 https://www.ncbi.nlm.nih.gov/pubmed/14626416

715 Bernichtein Sophie, Jomain Jean-Baptiste, Kelly Paul A, Goffin Vincent Molecular and cellular endocrinology. The N-terminus of human prolactin modulates its biological properties. 2003 Oct: 208(1-2) 11-21 https://www.ncbi.nlm.nih.gov/pubmed/14580717

716 Bateman O A, Sarra R, van Genesen S T, Kappé G, Lubsen N H, Slingsby C Experimental eye research. The stability of human acidic beta-crystallin oligomers and hetero-oligomers. 2003 Oct: 77(4) 409-22 https://www.ncbi.nlm.nih.gov/pubmed/12957141

717 Letourneur Odile, Ottone Sophie, Delauzun Vincent, Bastide Marie-Claire, Foussadier Agnès Protein expression and purification. Molecular cloning, overexpression in Escherichia coli, and purification of 6x his-tagged C-terminal domain of Clostridium difficile toxins A and B. 2003 Oct: 31(2) 276-85 https://www.ncbi.nlm.nih.gov/pubmed/14550648

718 Schauer Stefan, Lüer Corinna, Moser Jürgen Protein expression and purification. Large scale production of biologically active Escherichia coli glutamyl-tRNA reductase from inclusion bodies. 2003 Oct: 31(2) 271-5 https://www.ncbi.nlm.nih.gov/pubmed/14550647

719 Espinosa Joaquín M, Portal Daniel, Lobo Guillermo S, Pereira Claudio A, Alonso Guillermo D, Gómez Eliana B, Lan Graciela Humbert, Pomar Rolando V Rivera, Flawiá Mirtha M, Torres Héctor N Molecular and biochemical parasitology. Trypanosoma cruzi poly-zinc finger protein: a novel DNA/RNA-binding CCHC-zinc finger protein. 2003 Sep: 131(1) 35-44 https://www.ncbi.nlm.nih.gov/pubmed/12967710

720 Kirchhofer Daniel, Peek Mark, Li Wei, Stamos Jennifer, Eigenbrot Charles, Kadkhodayan Saloumeh, Elliott J Michael, Corpuz Racquel T, Lazarus Robert A, Moran Paul The Journal of biological chemistry. Tissue expression, protease specificity, and Kunitz domain functions of hepatocyte growth factor activator inhibitor-1B (HAI-1B), a new splice variant of HAI-1. 2003 Sep: 278(38) 36341-9 https://www.ncbi.nlm.nih.gov/pubmed/12815039

721 Aruna Battu, Ghosh Sudip, Singh Anil K, Mande Shekhar C, Srinivas V, Chauhan Radha, Ehtesham Nasreen Z Biochemistry. Human recombinant resistin protein displays a tendency to aggregate by forming intermolecular disulfide linkages. 2003 Sep: 42(36) 10554-9 https://www.ncbi.nlm.nih.gov/pubmed/12962478

722 Bruckner Robert C, Gunyuzlu Paul L, Stein Ross L Biochemistry. Coupled kinetics of ATP and peptide hydrolysis by Escherichia coli FtsH protease. 2003 Sep: 42(36) 10843-52 https://www.ncbi.nlm.nih.gov/pubmed/12962509

723 Clements Craig S, Reid Hugh H, Beddoe Travis, Tynan Fleur E, Perugini Matthew A, Johns Terrance G, Bernard Claude C A, Rossjohn Jamie Proceedings of the National Academy of Sciences of the United States of America. The crystal structure of myelin oligodendrocyte glycoprotein, a key autoantigen in multiple sclerosis. 2003 Sep: 100(19) 11059-64 https://www.ncbi.nlm.nih.gov/pubmed/12960396

724 Nishiyama Yoshitaka, Hardré-Liénard Hélène, Miras Stéphane, Miège Christine, Block Maryse A, Revah Frédéric, Joyard Jacques, Maréchal Eric Protein expression and purification. Refolding from denatured inclusion bodies, purification to homogeneity and simplified assay of MGDG synthases from land plants. 2003 Sep: 31(1) 79-87 https://www.ncbi.nlm.nih.gov/pubmed/12963344

725 Gasparian Marine E, Ostapchenko Valeriy G, Schulga Alexey A, Dolgikh Dmitry A, Kirpichnikov Mikhail P Protein expression and purification. Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli. 2003 Sep: 31(1) 133-9 https://www.ncbi.nlm.nih.gov/pubmed/12963350

726 Lee Hak-Joo, Im Hana Protein expression and purification. Purification of recombinant plasminogen activator inhibitor-1 in the active conformation by refolding from inclusion bodies. 2003 Sep: 31(1) 99-107 https://www.ncbi.nlm.nih.gov/pubmed/12963346

727 Ouellette Thomas, Destrau Sophie, Ouellette Timothy, Zhu Jianwei, Roach John M, Coffman J Daniel, Hecht Toby, Lynch James E, Giardina Steven L Protein expression and purification. Production and purification of refolded recombinant human IL-7 from inclusion bodies. 2003 Aug: 30(2) 156-66 https://www.ncbi.nlm.nih.gov/pubmed/12880763

728 Kim Eun Jeong, Park Hye Jung, Park Tai Hyun Biochemical and biophysical research communications. Inhibition of apoptosis by recombinant 30K protein originating from silkworm hemolymph. 2003 Aug: 308(3) 523-8 https://www.ncbi.nlm.nih.gov/pubmed/12914782

729 Chan Yuk Hang, Cheng Christopher H K, Chan King Ming Comparative biochemistry and physiology. Recombinant goldfish growth hormones (gfGH-I and -II) expressed in Escherichia coli have similar biological activities. 2003 Aug: Part A, Molecular & integrative physiology. 135(4) 613-24 https://www.ncbi.nlm.nih.gov/pubmed/12890551

730 Chung Christine, Varnerin Jeffrey P, Morin Nancy R, MacNeil Douglas J, Singh Suresh B, Patel Sangita, Scapin Giovanna, Van der Ploeg Lex H T, Tota Michael R Biochemical and biophysical research communications. The role of tryptophan 1072 in human PDE3B inhibitor binding. 2003 Aug: 307(4) 1045-50 https://www.ncbi.nlm.nih.gov/pubmed/12878217

731 Boeggeman Elizabeth E, Ramakrishnan Boopathy, Qasba Pradman K Protein expression and purification. The N-terminal stem region of bovine and human beta1,4-galactosyltransferase I increases the in vitro folding efficiency of their catalytic domain from inclusion bodies. 2003 Aug: 30(2) 219-29 https://www.ncbi.nlm.nih.gov/pubmed/12880771

732 Stancombe Patrick R, Alexander Frances C G, Ling Roger, Matheson Mary A, Shone Clifford C, Chaddock John A Protein expression and purification. Isolation of the gene and large-scale expression and purification of recombinant Erythrina cristagalli lectin. 2003 Aug: 30(2) 283-92 https://www.ncbi.nlm.nih.gov/pubmed/12880778

733 Johnston Jodie M, Arcus Vickery L, Morton Craig J, Parker Michael W, Baker Edward N Journal of bacteriology. Crystal structure of a putative methyltransferase from Mycobacterium tuberculosis: misannotation of a genome clarified by protein structural analysis. 2003 Jul: 185(14) 4057-65 https://www.ncbi.nlm.nih.gov/pubmed/12837779

734 Chang Te-Sheng, Wan Hisao-Ming, Chen Chien-Chiang, Giridhar R, Wu Wen-Teng Biotechnology letters. Fusion protein of the hyaluronan binding domain from human TSG-6 with luciferase for assay of hyaluronan. 2003 Jul: 25(13) 1037-40 https://www.ncbi.nlm.nih.gov/pubmed/12889811

735 Nikitin Dmitri, Mokrishcheva Marina, Denjmukhametov Marat, Pertzev Alexander, Zakharova Marina, Solonin Alexander Protein expression and purification. Construction of an overproducing strain, purification, and biochemical characterization of the 6His-Eco29kI restriction endonuclease. 2003 Jul: 30(1) 26-31 https://www.ncbi.nlm.nih.gov/pubmed/12821318

736 Harrowing Sarah R, Chaudhuri Julian B Journal of biochemical and biophysical methods. Effect of column dimensions and flow rates on size-exclusion refolding of beta-lactamase. 2003 Jun: 56(1-3) 177-88 https://www.ncbi.nlm.nih.gov/pubmed/12834976

737 Juan Chi-Chang, Kan Lou-Sing, Huang Cheng-Chih, Chen Shih-Shih, Ho Low-Tone, Au Lo-Chun Journal of biotechnology. Production and characterization of bioactive recombinant resistin in Escherichia coli. 2003 Jun: 103(2) 113-7 https://www.ncbi.nlm.nih.gov/pubmed/12814870

738 Yang Qing, Ching Wei-Mei, Jiang Ju, Lousteau Leslie, Richards Allen L Annals of the New York Academy of Sciences. An improved method for the purification and refolding of r56-kDa proteins from Gilliam and Kato strains of Orientia tsutsugamushi. 2003 Jun: 990 375-85 https://www.ncbi.nlm.nih.gov/pubmed/12860658

739 Baneres Jean-Louis, Martin Aimée, Hullot Pierre, Girard Jean-Pierre, Rossi Jean-Claude, Parello Joseph Journal of molecular biology. Structure-based analysis of GPCR function: conformational adaptation of both agonist and receptor upon leukotriene B4 binding to recombinant BLT1. 2003 Jun: 329(4) 801-14 https://www.ncbi.nlm.nih.gov/pubmed/12787679

740 Smith Vernon R, Walker John E Protein expression and purification. Purification and folding of recombinant bovine oxoglutarate/malate carrier by immobilized metal-ion affinity chromatography. 2003 Jun: 29(2) 209-16 https://www.ncbi.nlm.nih.gov/pubmed/12767811

741 Kaiser Liselotte, Grönlund Hans, Sandalova Tatyana, Ljunggren Hans-Gustaf, Schneider Gunter, van Hage-Hamsten Marianne, Achour Adnane Acta crystallographica. Production, crystallization and preliminary crystallographic study of the major cat allergen Fel d 1. 2003 Jun: Section D, Biological crystallography. 59(Pt 6) 1103-5 https://www.ncbi.nlm.nih.gov/pubmed/12777788

742 Aliberti Julio, Valenzuela Jesus G, Carruthers Vern B, Hieny Sara, Andersen John, Charest Hugues, Reis e Sousa Caetano, Fairlamb Alan, Ribeiro Jose M, Sher Alan Nature immunology. Molecular mimicry of a CCR5 binding-domain in the microbial activation of dendritic cells. 2003 May: 4(5) 485-90 https://www.ncbi.nlm.nih.gov/pubmed/12665855

743 Koo Ingrid Chou, Stephens Richard S The Journal of biological chemistry. A developmentally regulated two-component signal transduction system in Chlamydia. 2003 May: 278(19) 17314-9 https://www.ncbi.nlm.nih.gov/pubmed/12600998

744 Backer Marina V, Gaynutdinov Timur I, Gorshkova Inna I, Crouch Robert J, Hu Tao, Aloise Renee, Arab Mohamed, Przekop Kristen, Backer Joseph M Journal of controlled release : official journal of the Controlled Release Society. Humanized docking system for assembly of targeting drug delivery complexes. 2003 May: 89(3) 499-511 https://www.ncbi.nlm.nih.gov/pubmed/12737851

745 Burns James M, Flaherty Patrick R, Romero Margarita M, Weidanz William P Vaccine. Immunization against Plasmodium chabaudi malaria using combined formulations of apical membrane antigen-1 and merozoite surface protein-1. 2003 May: 21(17-18) 1843-52 https://www.ncbi.nlm.nih.gov/pubmed/12706668

746 Ferrandon Sébastien, Sterzenbach Torsten, Mersha Fana B, Xu Ming-Qun Biochimica et biophysica acta. A single surface tryptophan in the chitin-binding domain from Bacillus circulans chitinase A1 plays a pivotal role in binding chitin and can be modified to create an elutable affinity tag. 2003 Apr: 1621(1) 31-40 https://www.ncbi.nlm.nih.gov/pubmed/12667608

747 Shi Yong, Jiang Chunsun, Chen Quan, Tang Hong Biochemical and biophysical research communications. One-step on-column affinity refolding purification and functional analysis of recombinant human VDAC1. 2003 Apr: 303(2) 475-82 https://www.ncbi.nlm.nih.gov/pubmed/12659842

748 Epp Christian, Kauth Christian W, Bujard Hermann, Lutz Rolf Journal of chromatography. Expression and purification of Plasmodium falciparum MSP-1(42): A malaria vaccine candidate. 2003 Mar: B, Analytical technologies in the biomedical and life sciences. 786(1-2) 61-72 https://www.ncbi.nlm.nih.gov/pubmed/12651002

749 Ribeiro Euripedes A, Regis Wiliam C B, Tasic Ljubica, Ramos Carlos H I Protein expression and purification. Fast purification of the Apo form and of a non-binding heme mutant of recombinant sperm whale myoglobin. 2003 Mar: 28(1) 202-8 https://www.ncbi.nlm.nih.gov/pubmed/12651126

750 Umetsu Mitsuo, Tsumoto Kouhei, Hara Masaki, Ashish Kumar, Goda Shuichiro, Adschiri Tadafumi, Kumagai Izumi The Journal of biological chemistry. How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment. 2003 Mar: 278(11) 8979-87 https://www.ncbi.nlm.nih.gov/pubmed/12519771

751 Ferré Henrik, Ruffet Emmanuel, Blicher Thomas, Sylvester-Hvid Christina, Nielsen Lise Lotte B, Hobley Timothy J, Thomas Owen R T, Buus Søren Protein science : a publication of the Protein Society. Purification of correctly oxidized MHC class I heavy-chain molecules under denaturing conditions: a novel strategy exploiting disulfide assisted protein folding. 2003 Mar: 12(3) 551-9 https://www.ncbi.nlm.nih.gov/pubmed/12592025

752 Lemercier G, Bakalara N, Santarelli X Journal of chromatography. On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli. 2003 Mar: B, Analytical technologies in the biomedical and life sciences. 786(1-2) 305-9 https://www.ncbi.nlm.nih.gov/pubmed/12651027

753 Ito Mie, Nagata Koji, Kato Yusuke, Oda Yoshifumi, Yamagoe Satoshi, Suzuki Kazuo, Tanokura Masaru Protein expression and purification. Expression, oxidative refolding, and characterization of six-histidine-tagged recombinant human LECT2, a 16-kDa chemotactic protein with three disulfide bonds. 2003 Feb: 27(2) 272-8 https://www.ncbi.nlm.nih.gov/pubmed/12597887

754 Wang Jeng-Jie, Swaisgood Harold E, Shih Jason C H Biotechnology and bioengineering. Bioimmobilization of keratinase using Bacillus subtilis and Escherichia coli systems. 2003 Feb: 81(4) 421-9 https://www.ncbi.nlm.nih.gov/pubmed/12491527

755 Glynou Kyriaki, Ioannou Penelope C, Christopoulos Theodore K Protein expression and purification. One-step purification and refolding of recombinant photoprotein aequorin by immobilized metal-ion affinity chromatography. 2003 Feb: 27(2) 384-90 https://www.ncbi.nlm.nih.gov/pubmed/12597900

756 Kojima Yuzo, Kobayashi Michihiko, Shimizu Sakayu Journal of bioscience and bioengineering. A novel lipase from Pseudomonas fluorescens HU380: gene cloning, overproduction, renaturation-activation, two-step purification, and characterization. 2003 : 96(3) 242-9 https://www.ncbi.nlm.nih.gov/pubmed/16233516

757 Kearon Clive CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne. Diagnosis of pulmonary embolism. 2003 Jan: 168(2) 183-94 https://www.ncbi.nlm.nih.gov/pubmed/12538548

758 Cheng Donghang, Shen Qiang, Nan Fajun, Qian Zhen, Ye Qi-Zhuang Protein expression and purification. Purification and characterization of catalytic domains of gelatinase A with or without fibronectin insert for high-throughput inhibitor screening. 2003 Jan: 27(1) 63-74 https://www.ncbi.nlm.nih.gov/pubmed/12509986

759 Sakono Masafumi, Ichinose Hirofumi, Goto Masahiro Journal of bioscience and bioengineering. Protein refolding in nanostructured reversed micelles including a molecular chaperone. 2003 : 96(3) 275-8 https://www.ncbi.nlm.nih.gov/pubmed/16233521

760 Boucher Philip E, Maris Ann E, Yang Mei-Shin, Stibitz Scott Molecular cell. The response regulator BvgA and RNA polymerase alpha subunit C-terminal domain bind simultaneously to different faces of the same segment of promoter DNA. 2003 Jan: 11(1) 163-73 https://www.ncbi.nlm.nih.gov/pubmed/12535530

761 Shimada Yutaka, Maeda Masato, Watanabe Go, Yamasaki Seiji, Komoto Izumi, Kaganoi Junichi, Kan Takatsugu, Hashimoto Yosuke, Imoto Issei, Inazawa Johji, Imamura Masayuki Clinical cancer research : an official journal of the American Association for Cancer Research. Cell culture in esophageal squamous cell carcinoma and the association with molecular markers. 2003 Jan: 9(1) 243-9 https://www.ncbi.nlm.nih.gov/pubmed/12538476

762 Tang Liang, Morales Tony, Boroughs Karen L, Cailles Lo-Keiser Kathy, Sellins Karen, Stedman Kim, McCall Catherine, McDermott Martin J Molecular immunology. Expression and characterization of recombinant canine IL-13 receptor alpha2 protein and its biological activity in vitro. 2003 Jan: 39(12) 719-27 https://www.ncbi.nlm.nih.gov/pubmed/12531283

763 Fitches Elaine, Audsley Neil, Gatehouse John A, Edwards John P Insect biochemistry and molecular biology. Fusion proteins containing neuropeptides as novel insect contol agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion. 2002 Dec: 32(12) 1653-61 https://www.ncbi.nlm.nih.gov/pubmed/12429117

764 Cunliffe Sharon L, Wyer Jessica R, Sutton Julian K, Lucas Michaela, Harcourt Gillian, Klenerman Paul, McMichael Andrew J, Kelleher Anthony D European journal of immunology. Optimization of peptide linker length in production of MHC class II/peptide tetrameric complexes increases yield and stability, and allows identification of antigen-specific CD4+T cells in peripheral blood mononuclear cells. 2002 Dec: 32(12) 3366-75 https://www.ncbi.nlm.nih.gov/pubmed/12432567

765 Backer Marina V, Gaynutdinov Timur I, Aloise Renee, Przekop Kristen, Backer Joseph M Protein expression and purification. Engineering S-protein fragments of bovine ribonuclease A for targeted drug delivery. 2002 Dec: 26(3) 455-61 https://www.ncbi.nlm.nih.gov/pubmed/12460770

766 Bacher Susanne, Achatz Gernot, Schmitz M L, Lamers Marinus C Biochimie. Prohibitin and prohibitone are contained in high-molecular weight complexes and interact with alpha-actinin and annexin A2. 2002 Dec: 84(12) 1207-20 https://www.ncbi.nlm.nih.gov/pubmed/12628297

767 Clements Craig S, Kjer-Nielsen Lars, MacDonald Whitney A, Brooks Andrew G, Purcell Anthony W, McCluskey James, Rossjohn Jamie Acta crystallographica. The production, purification and crystallization of a soluble heterodimeric form of a highly selected T-cell receptor in its unliganded and liganded state. 2002 Dec: Section D, Biological crystallography. 58(Pt 12) 2131-4 https://www.ncbi.nlm.nih.gov/pubmed/12454477

768 Kim Yong-Tae, Downs Deborah, Wu Shili, Dashti Azar, Pan Yujun, Zhai Peng, Wang Xinjuan, Zhang Xuejun C, Lin Xinli European journal of biochemistry / FEBS. Enzymic properties of recombinant BACE2. 2002 Nov: 269(22) 5668-77 https://www.ncbi.nlm.nih.gov/pubmed/12423367

769 de Jonge Marien I, Bos Martine P, Hamstra Hendrik J, Jiskoot Wim, van Ulsen Peter, Tommassen Jan, van Alphen Loek, van der Ley Peter European journal of biochemistry / FEBS. Conformational analysis of opacity proteins from Neisseria meningitidis. 2002 Nov: 269(21) 5215-23 https://www.ncbi.nlm.nih.gov/pubmed/12392553

770 Fraser Deborah A, Harris Claire L, Smith Richard A G, Morgan B Paul Protein science : a publication of the Protein Society. Bacterial expression and membrane targeting of the rat complement regulator Crry: a new model anticomplement therapeutic. 2002 Oct: 11(10) 2512-21 https://www.ncbi.nlm.nih.gov/pubmed/12237472

771 Cantoni Claudia, Ponassi Marco, Biassoni Roberto, Conte Romana, Spallarossa Andrea, Moretta Alessandro, Moretta Lorenzo, Bolognesi Martino, Bordo Domenico Acta crystallographica. Crystallization and preliminary crystallographic characterization of the extracellular Ig-like domain of human natural killer cell activating receptor NKp44. 2002 Oct: Section D, Biological crystallography. 58(Pt 10 Pt 2) 1843-5 https://www.ncbi.nlm.nih.gov/pubmed/12351833

772 Hopper David J, Kaderbhai Mustak A, Marriott Shirley A, Young Michael, Rogozinski Jerzy The Biochemical journal. Cloning, sequencing and heterologous expression of the gene for lupanine hydroxylase, a quinocytochrome c from a Pseudomonas sp. 2002 Oct: 367(Pt 2) 483-9 https://www.ncbi.nlm.nih.gov/pubmed/12119046

773 Macdonald Whitney, Williams David S, Clements Craig S, Gorman Jeffery J, Kjer-Nielsen Lars, Brooks Andrew G, McCluskey James, Rossjohn Jamie, Purcell Anthony W FEBS letters. Identification of a dominant self-ligand bound to three HLA B44 alleles and the preliminary crystallographic analysis of recombinant forms of each complex. 2002 Sep: 527(1-3) 27-32 https://www.ncbi.nlm.nih.gov/pubmed/12220628

774 Asano Ryutaro, Kudo Toshio, Makabe Koki, Tsumoto Kouhei, Kumagai Izumi FEBS letters. Antitumor activity of interleukin-21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli. 2002 Sep: 528(1-3) 70-6 https://www.ncbi.nlm.nih.gov/pubmed/12297282

775 Adir Noam, Rukhman Valeria, Brumshtein Boris, Anati Rina Acta crystallographica. Preliminary X-ray crystallographic analysis of a soluble form of MntC, a periplasmic manganese-binding component of an ABC-type Mn transporter from Synechocystis sp. PCC 6803. 2002 Sep: Section D, Biological crystallography. 58(Pt 9) 1476-8 https://www.ncbi.nlm.nih.gov/pubmed/12198307

776 Jekabsons Mika B, Echtay Karim S, Brand Martin D The Biochemical journal. Nucleotide binding to human uncoupling protein-2 refolded from bacterial inclusion bodies. 2002 Sep: 366(Pt 2) 565-71 https://www.ncbi.nlm.nih.gov/pubmed/12030845

777 Arêas Ana Paula de Mattos, Oliveira Maria Leonor Sarno de, Ramos Celso Raul Romero, Sbrogio-Almeida Maria Elisabete, Raw Isaías, Ho Paulo Lee Protein expression and purification. Synthesis of cholera toxin B subunit gene: cloning and expression of a functional 6XHis-tagged protein in Escherichia coli. 2002 Aug: 25(3) 481-7 https://www.ncbi.nlm.nih.gov/pubmed/12182829

778 Lewin Allison C, Doughty Phillip A, Flegg Lynda, Moore Geoffrey R, Spiro Stephen Microbiology (Reading, England). The ferric uptake regulator of Pseudomonas aeruginosa has no essential cysteine residues and does not contain a structural zinc ion. 2002 Aug: 148(Pt 8) 2449-56 https://www.ncbi.nlm.nih.gov/pubmed/12177338

779 Bergendahl Veit, Anthony Larry C, Heyduk Tomasz, Burgess Richard R Analytical biochemistry. On-column tris(2-carboxyethyl)phosphine reduction and IC5-maleimide labeling during purification of a RpoC fragment on a nickel-nitrilotriacetic acid Column. 2002 Aug: 307(2) 368-74 https://www.ncbi.nlm.nih.gov/pubmed/12202256

780 Mitsuda Yuuichi, Takimoto Akio, Kamitani Shigeki, Kitamura Kazuo, Sakata Tsuneaki, Mitsushima Kenji Protein expression and purification. Large-scale production of functional human adrenomedullin: expression, cleavage, amidation, and purification. 2002 Aug: 25(3) 448-55 https://www.ncbi.nlm.nih.gov/pubmed/12182825

781 Hwang Hyo-Sung, Chung Hye-Shin Protein expression and purification. Preparation of active recombinant cathepsin K expressed in bacteria as inclusion body. 2002 Aug: 25(3) 541-6 https://www.ncbi.nlm.nih.gov/pubmed/12182837

782 Goyal Anita, Seth Divya, Batra Janendra K Biochemical and biophysical research communications. Role of cis prolines 112 and 126 in the functional activity of ribonucleolytic toxin restrictocin. 2002 Jul: 295(4) 812-7 https://www.ncbi.nlm.nih.gov/pubmed/12127966

783 Han Bo, Perelman Natalya, Tang Baowei, Hall Fredrick, Shors Edwin C, Nimni Marcel E Journal of orthopaedic research : official publication of the Orthopaedic Research Society. Collagen-targeted BMP3 fusion proteins arrayed on collagen matrices or porous ceramics impregnated with Type I collagen enhance osteogenesis in a rat cranial defect model. 2002 Jul: 20(4) 747-55 https://www.ncbi.nlm.nih.gov/pubmed/12168663

784 Króliczewski Jarosław, Szczepaniak Andrzej Biochimica et biophysica acta. In vitro reconstitution of the spinach chloroplast cytochrome b6 protein from a fusion protein expressed in Escherichia coli. 2002 Jul: 1598(1-2) 177-84 https://www.ncbi.nlm.nih.gov/pubmed/12147358

785 Boeggeman Elizabeth, Qasba Pradman K Glycobiology. Studies on the metal binding sites in the catalytic domain of beta1,4-galactosyltransferase. 2002 Jul: 12(7) 395-407 https://www.ncbi.nlm.nih.gov/pubmed/12122021

786 Yamasaki Hiroshi, Mineki Reiko, Murayama Kimie, Ito Akira, Aoki Takashi International journal for parasitology. Characterisation and expression of the Fasciola gigantica cathepsin L gene. 2002 Jul: 32(8) 1031-42 https://www.ncbi.nlm.nih.gov/pubmed/12076632

787 Menzella Hugo G, Gramajo Hugo C, Ceccarelli Eduardo A Protein expression and purification. High recovery of prochymosin from inclusion bodies using controlled air oxidation. 2002 Jul: 25(2) 248-55 https://www.ncbi.nlm.nih.gov/pubmed/12135557

788 Dutta Sheetij, Lalitha P V, Ware Lisa A, Barbosa Arnoldo, Moch J Kathleen, Vassell Meredith A, Fileta Bader B, Kitov Svetlana, Kolodny Nelly, Heppner D Gray, Haynes J David, Lanar David E Infection and immunity. Purification, characterization, and immunogenicity of the refolded ectodomain of the Plasmodium falciparum apical membrane antigen 1 expressed in Escherichia coli. 2002 Jun: 70(6) 3101-10 https://www.ncbi.nlm.nih.gov/pubmed/12011004

789 Richter Wito, Hermsdorf Thomas, Kronbach Thomas, Dettmer Dietrich Protein expression and purification. Refolding and purification of recombinant human PDE7A expressed in Escherichia coli as inclusion bodies. 2002 Jun: 25(1) 138-48 https://www.ncbi.nlm.nih.gov/pubmed/12071709

790 Zhang Xuewu, Schwartz Jean-Claude D, Almo Steven C, Nathenson Stanley G Protein expression and purification. Expression, refolding, purification, molecular characterization, crystallization, and preliminary X-ray analysis of the receptor binding domain of human B7-2. 2002 Jun: 25(1) 105-13 https://www.ncbi.nlm.nih.gov/pubmed/12071705

791 Lee Myung-Hoon, Park Tae-In, Park Yong-Bok, Kwak Ju-Won Protein expression and purification. Bacterial expression and in vitro refolding of a single-chain fv antibody specific for human plasma apolipoprotein B-100. 2002 Jun: 25(1) 166-73 https://www.ncbi.nlm.nih.gov/pubmed/12071712

792 Saini Deepak Kumar, Pant Neha, Das Taposh K, Tyagi Jaya Sivaswami Protein expression and purification. Cloning, overexpression, purification, and matrix-assisted refolding of DevS (Rv 3132c) histidine protein kinase of Mycobacterium tuberculosis. 2002 Jun: 25(1) 203-8 https://www.ncbi.nlm.nih.gov/pubmed/12071717

793 Alexandrov Kirill, Heinemann Ines, Durek Thomas, Sidorovitch Vadim, Goody Roger S, Waldmann Herbert Journal of the American Chemical Society. Intein-mediated synthesis of geranylgeranylated Rab7 protein in vitro. 2002 May: 124(20) 5648-9 https://www.ncbi.nlm.nih.gov/pubmed/12010032

794 Bazarsuren Ariuna, Grauschopf Ulla, Wozny Manfred, Reusch Dietmar, Hoffmann Eike, Schaefer Wolfgang, Panzner Steffen, Rudolph Rainer Biophysical chemistry. In vitro folding, functional characterization, and disulfide pattern of the extracellular domain of human GLP-1 receptor. 2002 May: 96(2-3) 305-18 https://www.ncbi.nlm.nih.gov/pubmed/12034449

795 Chang Chia-Ching, Tsai Chi-Tung, Chang Chi-Yao Protein engineering. Structural restoration of inactive recombinant fish growth hormones by chemical chaperonin and solvent restraint approaches. 2002 May: 15(5) 437-41 https://www.ncbi.nlm.nih.gov/pubmed/12034864

796 Bell Stefan, Hansen Silke, Buchner Johannes Biophysical chemistry. Refolding and structural characterization of the human p53 tumor suppressor protein. 2002 May: 96(2-3) 243-57 https://www.ncbi.nlm.nih.gov/pubmed/12034444

797 Belorgey Didier, Crowther Damian C, Mahadeva Ravi, Lomas David A The Journal of biological chemistry. Mutant Neuroserpin (S49P) that causes familial encephalopathy with neuroserpin inclusion bodies is a poor proteinase inhibitor and readily forms polymers in vitro. 2002 May: 277(19) 17367-73 https://www.ncbi.nlm.nih.gov/pubmed/11880376

798 Lin Li-Jen, Tai Sorgan S K, Peng Chi-Chung, Tzen Jason T C Plant physiology. Steroleosin, a sterol-binding dehydrogenase in seed oil bodies. 2002 Apr: 128(4) 1200-11 https://www.ncbi.nlm.nih.gov/pubmed/11950969

799 Camacho Mónica, Rodríguez-Arnedo Adoración, Bonete María José FEMS microbiology letters. NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii: cloning, sequence determination and overexpression in Escherichia coli. 2002 Apr: 209(2) 155-60 https://www.ncbi.nlm.nih.gov/pubmed/12007799

800 Calderone Vito, Trabucco Michela, Menin Valentina, Negro Alessandro, Zanotti Giuseppe Biochimica et biophysica acta. Cloning of human 3-hydroxyanthranilic acid dioxygenase in Escherichia coli: characterisation of the purified enzyme and its in vitro inhibition by Zn2+. 2002 Apr: 1596(2) 283-92 https://www.ncbi.nlm.nih.gov/pubmed/12007609

801 Dames Sonja A, Martinez-Yamout Maria, De Guzman Roberto N, Dyson H Jane, Wright Peter E Proceedings of the National Academy of Sciences of the United States of America. Structural basis for Hif-1 alpha /CBP recognition in the cellular hypoxic response. 2002 Apr: 99(8) 5271-6 https://www.ncbi.nlm.nih.gov/pubmed/11959977

802 Beglova Natalia, Blacklow Stephen C, Takagi Junichi, Springer Timothy A Nature structural biology. Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation. 2002 Apr: 9(4) 282-7 https://www.ncbi.nlm.nih.gov/pubmed/11896403

803 Panagabko Candace, Morley Samantha, Neely Steve, Lei Huangshu, Manor Danny, Atkinson Jeffrey Protein expression and purification. Expression and refolding of recombinant human alpha-tocopherol transfer protein capable of specific alpha-tocopherol binding. 2002 Apr: 24(3) 395-403 https://www.ncbi.nlm.nih.gov/pubmed/11922755

804 Khumthong Rabuesak, Angsuthanasombat Chanan, Panyim Sakol, Katzenmeier Gerd Journal of biochemistry and molecular biology. In vitro determination of dengue virus type 2 NS2B-NS3 protease activity with fluorescent peptide substrates. 2002 Mar: 35(2) 206-12 https://www.ncbi.nlm.nih.gov/pubmed/12297031

805 Douglas Justin T, von Haller Priska D, Gehrmann Marion, Llinás Miguel, Schaller Johann Biochemistry. The two-domain NK1 fragment of plasminogen: folding, ligand binding, and thermal stability profile. 2002 Mar: 41(10) 3302-10 https://www.ncbi.nlm.nih.gov/pubmed/11876638

806 Shin Hyun Hee, Lee Moo Hyung, Kim Suk Gi, Lee Yun Hwa, Kwon Byoung S, Choi Hye Seon FEBS letters. Recombinant glucocorticoid induced tumor necrosis factor receptor (rGITR) induces NOS in murine macrophage. 2002 Mar: 514(2-3) 275-80 https://www.ncbi.nlm.nih.gov/pubmed/11943165

807 Raver Nina, Vardy Eyal, Livnah Oded, Devos Rene, Gertler Arieh General and comparative endocrinology. Comparison of R128Q mutations in human, ovine, and chicken leptins. 2002 Mar: 126(1) 52-8 https://www.ncbi.nlm.nih.gov/pubmed/11944966

808 Vallejo Luis Felipe, Brokelmann Maren, Marten Sabine, Trappe Susanne, Cabrera-Crespo Joaquin, Hoffmann Andrea, Gross Gerhard, Weich Herbert A, Rinas Ursula Journal of biotechnology. Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli. 2002 Mar: 94(2) 185-94 https://www.ncbi.nlm.nih.gov/pubmed/11796171

809 Koo Hyun Min, Kim Joo-Hyon, Hwang In Kwan, Lee Seo-Jin, Kim Tae-Han, Rhee Ki-Hyeong, Lee Seung-Taek Molecules and cells. Refolding of the catalytic and hinge domains of human MT1-mMP expressed in Escherichia coli and its characterization. 2002 Feb: 13(1) 118-24 https://www.ncbi.nlm.nih.gov/pubmed/11911461

810 Cheng Dong-Hang, Shen Qiang, Qian Jing, Qian Zhen, Ye Qi-Zhuang Acta pharmacologica Sinica. Expression and purification of catalytic domain of human macrophage elastase for high throughput inhibitor screening. 2002 Feb: 23(2) 143-51 https://www.ncbi.nlm.nih.gov/pubmed/11866875

811 Yang Junhao, Koga Yuichi, Nakano Hideo, Yamane Tsuneo Protein engineering. Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding site. 2002 Feb: 15(2) 147-52 https://www.ncbi.nlm.nih.gov/pubmed/11917151

812 Bhakta Sanjib, Basu Joyoti The Biochemical journal. Overexpression, purification and biochemical characterization of a class A high-molecular-mass penicillin-binding protein (PBP), PBP1* and its soluble derivative from Mycobacterium tuberculosis. 2002 Feb: 361(Pt 3) 635-9 https://www.ncbi.nlm.nih.gov/pubmed/11802794

813 Mark Buswell A, Ebtinger Mark, Vertés Alain A, Middelberg Anton P J Biotechnology and bioengineering. Effect of operating variables on the yield of recombinant trypsinogen for a pulse-fed dilution-refolding reactor. 2002 Feb: 77(4) 435-44 https://www.ncbi.nlm.nih.gov/pubmed/11787016

814 Boudko Sergei P, Londer Yuri Y, Letarov Andrei V, Sernova Natalia V, Engel Juergen, Mesyanzhinov Vadim V European journal of biochemistry / FEBS. Domain organization, folding and stability of bacteriophage T4 fibritin, a segmented coiled-coil protein. 2002 Feb: 269(3) 833-41 https://www.ncbi.nlm.nih.gov/pubmed/11846809

815 Ayora Silvia, Piruat José I, Luna Rosa, Reiss Bernd, Russo Vincenzo E A, Aguilera Andrés, Alonso Juan C Journal of molecular biology. Characterization of two highly similar Rad51 homologs of Physcomitrella patens. 2002 Feb: 316(1) 35-49 https://www.ncbi.nlm.nih.gov/pubmed/11829501

816 Sanghera Narinder, Pinheiro Teresa J T Journal of molecular biology. Binding of prion protein to lipid membranes and implications for prion conversion. 2002 Feb: 315(5) 1241-56 https://www.ncbi.nlm.nih.gov/pubmed/11827491

817 Fu Ling, Liang Jack J N FEBS letters. Conformational change and destabilization of cataract gammaC-crystallin T5P mutant. 2002 Feb: 513(2-3) 213-6 https://www.ncbi.nlm.nih.gov/pubmed/11904153

818 He Hua-Jun, Yuan Qin-Sheng, Yang Guan-Zhen, Wu Xiang-Fu Protein expression and purification. High-level expression of human extracellular superoxide dismutase in Escherichia coli and insect cells. 2002 Feb: 24(1) 13-7 https://www.ncbi.nlm.nih.gov/pubmed/11812217

819 Kwon Hyun-Ju, Haruki Mitsuru, Morikawa Masaaki, Omori Kenji, Kanaya Shigenori Journal of bioscience and bioengineering. Role of repetitive nine-residue sequence motifs in secretion, enzymatic activity, and protein conformation of a family I.3 lipase. 2002 : 93(2) 157-64 https://www.ncbi.nlm.nih.gov/pubmed/16233181

820 Guo Y W, Chang T Y, Lin K T, Liu H W, Shih K C, Cheng S H Protein expression and purification. Cloning and functional expression of the mucrosobin protein, a beta-fibrinogenase of Trimeresurus mucrosquamatus (Taiwan Habu). 2001 Dec: 23(3) 483-90 https://www.ncbi.nlm.nih.gov/pubmed/11722187

821 Kimbita E N, Xuan X, Huang X, Miyazawa T, Fukumoto S, Mishima M, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Suzuki N, Mikami T, Igarashi I Veterinary parasitology. Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1. 2001 Dec: 102(1-2) 35-44 https://www.ncbi.nlm.nih.gov/pubmed/11705650

822 Ferrer J, Fisher M, Burke J, Sedelnikova S E, Baker P J, Gilmour D J, Bonete M J, Pire C, Esclapez J, Rice D W Acta crystallographica. Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Haloferax mediterranei. 2001 Dec: Section D, Biological crystallography. 57(Pt 12) 1887-9 https://www.ncbi.nlm.nih.gov/pubmed/11717506

823 St John R J, Carpenter J F, Balny C, Randolph T W The Journal of biological chemistry. High pressure refolding of recombinant human growth hormone from insoluble aggregates. Structural transformations, kinetic barriers, and energetics. 2001 Dec: 276(50) 46856-63 https://www.ncbi.nlm.nih.gov/pubmed/11591710

824 Chang S Y, Tsai P C, Tseng C S, Liang P H Protein expression and purification. Refolding and characterization of a yeast dehydrodolichyl diphosphate synthase overexpressed in Escherichia coli. 2001 Dec: 23(3) 432-9 https://www.ncbi.nlm.nih.gov/pubmed/11722180

825 Takayama T K, McMullen B A, Nelson P S, Matsumura M, Fujikawa K Biochemistry. Characterization of hK4 (prostase), a prostate-specific serine protease: activation of the precursor of prostate specific antigen (pro-PSA) and single-chain urokinase-type plasminogen activator and degradation of prostatic acid phosphatase. 2001 Dec: 40(50) 15341-8 https://www.ncbi.nlm.nih.gov/pubmed/11735417

826 Huang J L, Huang J H, Shyu R H, Teng C W, Lin Y L, Kuo M D, Yao C W, Shaio M F Journal of medical virology. High-level expression of recombinant dengue viral NS-1 protein and its potential use as a diagnostic antigen. 2001 Nov: 65(3) 553-60 https://www.ncbi.nlm.nih.gov/pubmed/11596093

827 Yamagata A, Masui R, Kakuta Y, Kuramitsu S, Fukuyama K Nucleic acids research. Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain. 2001 Nov: 29(22) 4617-24 https://www.ncbi.nlm.nih.gov/pubmed/11713311

828 Grimm K M, Trigona W L, Heidecker G J, Joyce J G, Fu T M, Shiver J W, Keller P M, Cook J C Protein expression and purification. An enhanced and scalable process for the purification of SIV Gag-specific MHC tetramer. 2001 Nov: 23(2) 270-81 https://www.ncbi.nlm.nih.gov/pubmed/11676602

829 Chen H P, Wu S H, Lin Y L, Chen C M, Tsay S S The Journal of biological chemistry. Cloning, sequencing, heterologous expression, purification, and characterization of adenosylcobalamin-dependent D-ornithine aminomutase from Clostridium sticklandii. 2001 Nov: 276(48) 44744-50 https://www.ncbi.nlm.nih.gov/pubmed/11577113

830 Kolodny N, Kitov S, Vassell M A, Miller V L, Ware L A, Fegeding K, De La Vega P, Sacci J B, Lanar D E Journal of chromatography. Two-step chromatographic purification of recombinant Plasmodium falciparum circumsporozoite protein from Escherichia coli. 2001 Oct: B, Biomedical sciences and applications. 762(1) 77-86 https://www.ncbi.nlm.nih.gov/pubmed/11589461

831 Chen X G, Gong Y, Hua-Li , Lun Z R, Fung M C Protein expression and purification. High-level expression and purification of immunogenic recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli. 2001 Oct: 23(1) 33-7 https://www.ncbi.nlm.nih.gov/pubmed/11570843

832 Charbonnier F, Köhler T, Pechère J C, Ducruix A Protein expression and purification. Overexpression, refolding, and purification of the histidine-tagged outer membrane efflux protein OprM of Pseudomonas aeruginosa. 2001 Oct: 23(1) 121-7 https://www.ncbi.nlm.nih.gov/pubmed/11570853

833 Leibovich H, Raver N, Herman A, Gregoraszczuk E L, Gootwine E, Gertler A Protein expression and purification. Large-scale preparation of recombinant ovine prolactin and determination of its in vitro and in vivo activity. 2001 Aug: 22(3) 489-96 https://www.ncbi.nlm.nih.gov/pubmed/11483014

834 Rehm B H, Qi Q, Beermann B B, Hinz H J, Steinbüchel A The Biochemical journal. Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli. 2001 Aug: 358(Pt 1) 263-8 https://www.ncbi.nlm.nih.gov/pubmed/11485576

835 Degger B, Richardson N, Collet C, Upton Z General and comparative endocrinology. Production, in vitro characterisation, in vivo clearance, and tissue localisation of recombinant barramundi (Lates calcarifer) insulin-like growth factor II. 2001 Jul: 123(1) 38-50 https://www.ncbi.nlm.nih.gov/pubmed/11551116

836 Gaur D, Swaminathan S, Batra J K The Journal of biological chemistry. Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor. Generation of inhibitor-resistant cytotoxic variants. 2001 Jul: 276(27) 24978-84 https://www.ncbi.nlm.nih.gov/pubmed/11342552

837 Becker-Hapak M, McAllister S S, Dowdy S F Methods (San Diego, Calif. TAT-mediated protein transduction into mammalian cells. 2001 Jul:). 24(3) 247-56 https://www.ncbi.nlm.nih.gov/pubmed/11403574

838 Holloway D E, Hares M C, Shapiro R, Subramanian V, Acharya K R Protein expression and purification. High-level expression of three members of the murine angiogenin family in Escherichia coli and purification of the recombinant proteins. 2001 Jul: 22(2) 307-17 https://www.ncbi.nlm.nih.gov/pubmed/11437607

839 Juri Ayub M, Levin M J, Aguilar C F Protein expression and purification. Overexpression and refolding of the hydrophobic ribosomal P0 protein from Trypanosoma cruzi: a component of the P1/P2/P0 complex. 2001 Jul: 22(2) 225-33 https://www.ncbi.nlm.nih.gov/pubmed/11437598

840 Sijwali P S, Brinen L S, Rosenthal P J Protein expression and purification. Systematic optimization of expression and refolding of the Plasmodium falciparum cysteine protease falcipain-2. 2001 Jun: 22(1) 128-34 https://www.ncbi.nlm.nih.gov/pubmed/11388810

841 Park J H, Lee H H, Na S Y, Ju S K, Lee Y J, Lee M K, Kim K L Protein expression and purification. Recombinant expression of biologically active rat leptin in Escherichia coli. 2001 Jun: 22(1) 60-9 https://www.ncbi.nlm.nih.gov/pubmed/11388800

842 Hahm M S, Chung B H Protein expression and purification. Refolding and purification of yeast carboxypeptidase Y expressed as inclusion bodies in Escherichia coli. 2001 Jun: 22(1) 101-7 https://www.ncbi.nlm.nih.gov/pubmed/11388806

843 Singh S, Pandey K, Chattopadhayay R, Yazdani S S, Lynn A, Bharadwaj A, Ranjan A, Chitnis C The Journal of biological chemistry. Biochemical, biophysical, and functional characterization of bacterially expressed and refolded receptor binding domain of Plasmodium vivax duffy-binding protein. 2001 May: 276(20) 17111-6 https://www.ncbi.nlm.nih.gov/pubmed/11279211

844 Hodder A N, Crewther P E, Anders R F Infection and immunity. Specificity of the protective antibody response to apical membrane antigen 1. 2001 May: 69(5) 3286-94 https://www.ncbi.nlm.nih.gov/pubmed/11292751

845 Holmes W D, Consler T G, Dallas W S, Rocque W J, Willard D H Protein expression and purification. Solution studies of recombinant human stromal-cell-derived factor-1. 2001 Apr: 21(3) 367-77 https://www.ncbi.nlm.nih.gov/pubmed/11281710

846 Li M, Bullock C M, Knauer D J, Ehlert F J, Zhou Q Y Molecular pharmacology. Identification of two prokineticin cDNAs: recombinant proteins potently contract gastrointestinal smooth muscle. 2001 Apr: 59(4) 692-8 https://www.ncbi.nlm.nih.gov/pubmed/11259612

847 Grigorenko V, Andreeva I, Börchers T, Spener F, Egorov A Analytical chemistry. A genetically engineered fusion protein with horseradish peroxidase as a marker enzyme for use in competitive immunoassays. 2001 Mar: 73(6) 1134-9 https://www.ncbi.nlm.nih.gov/pubmed/11305642

848 Huang C C, Li L T, Shen M C, Chen J Y, Lin S W Thrombosis research. Domain specific monoclonal anti-factor VIII antibodies generated by inclusion body-renatured factor VIII peptides. 2001 Mar: 101(5) 405-15 https://www.ncbi.nlm.nih.gov/pubmed/11297757

849 Ishibashi M, Tokunaga H, Arakawa T, Tokunaga M Protein expression and purification. Expression, purification, and characterization of the active immunoglobulin-like domain of human granulocyte-colony-stimulating factor receptor in Escherichia coli. 2001 Mar: 21(2) 317-22 https://www.ncbi.nlm.nih.gov/pubmed/11237694

850 Agrawal S, Gupta D, Panda S K Virology. The 3' end of hepatitis E virus (HEV) genome binds specifically to the viral RNA-dependent RNA polymerase (RdRp). 2001 Mar: 282(1) 87-101 https://www.ncbi.nlm.nih.gov/pubmed/11259193

851 Llera A S, Viedma F, Sánchez-Madrid F, Tormo J The Journal of biological chemistry. Crystal structure of the C-type lectin-like domain from the human hematopoietic cell receptor CD69. 2001 Mar: 276(10) 7312-9 https://www.ncbi.nlm.nih.gov/pubmed/11036086

852 Fu J Y, Lyga A, Shi H, Blue M L, Dixon B, Chen D Protein expression and purification. Cloning, expression, purification, and characterization of rat MMP-12. 2001 Mar: 21(2) 268-74 https://www.ncbi.nlm.nih.gov/pubmed/11237688

853 Altamirano M M, Woolfson A, Donda A, Shamshiev A, Briseño-Roa L, Foster N W, Veprintsev D B, De Libero G, Fersht A R, Milstein C Proceedings of the National Academy of Sciences of the United States of America. Ligand-independent assembly of recombinant human CD1 by using oxidative refolding chromatography. 2001 Mar: 98(6) 3288-93 https://www.ncbi.nlm.nih.gov/pubmed/11248071

854 Lu H, Zhang H, Wang Q, Yuan H, He W, Zhao Z, Li Y Current microbiology. Purification, refolding of hybrid hIFNgamma-kringle 5 expressed in Escherichia coli. 2001 Mar: 42(3) 211-6 https://www.ncbi.nlm.nih.gov/pubmed/11270657

855 Zhu H, Liu W, Shi W, Xue Y, Kuai L, Ma Z Chinese medical journal. In vitro renaturation of recombinant human pro-urokinase expressed in Escherichia coli. 2001 Feb: 114(2) 186-90 https://www.ncbi.nlm.nih.gov/pubmed/11780204

856 Hartleib J, Rüterjans H Protein expression and purification. High-yield expression, purification, and characterization of the recombinant diisopropylfluorophosphatase from Loligo vulgaris. 2001 Feb: 21(1) 210-9 https://www.ncbi.nlm.nih.gov/pubmed/11162408

857 Ng C K, Huxtable S, Xu P, Hsieh D P Protein expression and purification. Purification and in vitro folding of recombinant human thrombopoietin receptor expressed in Escherichia coli. 2001 Feb: 21(1) 129-33 https://www.ncbi.nlm.nih.gov/pubmed/11162397

858 Ward R J, de Oliveira A H, Bortoleto R K, Rosa J C, Faça V M, Greene L J Protein expression and purification. Refolding and purification of Bothropstoxin-I, a Lys49-phospholipase A2 homologue, expressed as inclusion bodies in Escherichia coli. 2001 Feb: 21(1) 134-40 https://www.ncbi.nlm.nih.gov/pubmed/11162398

859 Levy I, Shoseyov O Journal of peptide science : an official publication of the European Peptide Society. Expression, refolding and indirect immobilization of horseradish peroxidase (HRP) to cellulose via a phage-selected peptide and cellulose-binding domain (CBD). 2001 Jan: 7(1) 50-7 https://www.ncbi.nlm.nih.gov/pubmed/11245205

860 Takai T, Okumura K, Ra C Bioscience, biotechnology, and biochemistry. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. 2001 Jan: 65(1) 79-85 https://www.ncbi.nlm.nih.gov/pubmed/11272849

861 Mao H, Gunasekera A H, Fesik S W Protein expression and purification. Expression, refolding, and isotopic labeling of human serum albumin domains for NMR spectroscopy. 2000 Dec: 20(3) 492-9 https://www.ncbi.nlm.nih.gov/pubmed/11087689

862 Patra A K, Gahlay G K, Reddy B V, Gupta S K, Panda A K European journal of biochemistry / FEBS. Refolding, structural transition and spermatozoa-binding of recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-C expressed in Escherichia coli. 2000 Dec: 267(24) 7075-81 https://www.ncbi.nlm.nih.gov/pubmed/11106418

863 Guo L, Wang J, Qian S, Yan X, Chen R, Meng G Biotechnology and bioengineering. Construction and structural modeling of a single-chain Fv-asparaginase fusion protein resistant to proteolysis. 2000 Nov: 70(4) 456-63 https://www.ncbi.nlm.nih.gov/pubmed/11005928

864 Llano E, Pendás A M, Aza-Blanc P, Kornberg T B, López-Otín C The Journal of biological chemistry. Dm1-MMP, a matrix metalloproteinase from Drosophila with a potential role in extracellular matrix remodeling during neural development. 2000 Nov: 275(46) 35978-85 https://www.ncbi.nlm.nih.gov/pubmed/10964925

865 Burns J M, Belk C C, Dunn P D Infection and immunity. A protective glycosylphosphatidylinositol-anchored membrane protein of Plasmodium yoelii trophozoites and merozoites contains two epidermal growth factor-like domains. 2000 Nov: 68(11) 6189-95 https://www.ncbi.nlm.nih.gov/pubmed/11035724

866 Tang S J, Sun K H, Sun G H, Chang T Y, Lee G C Protein expression and purification. Recombinant expression of the Candida rugosa lip4 lipase in Escherichia coli. 2000 Nov: 20(2) 308-13 https://www.ncbi.nlm.nih.gov/pubmed/11049754

867 Parkar A A, Stow M D, Smith K, Panicker A K, Guilloteau J P, Jupp R, Crowe S J Protein expression and purification. Large-scale expression, refolding, and purification of the catalytic domain of human macrophage metalloelastase (MMP-12) in Escherichia coli. 2000 Nov: 20(2) 152-61 https://www.ncbi.nlm.nih.gov/pubmed/11049739

868 Eisenmesser E Z, Kapust R B, Nawrocki J P, Mazzulla M J, Pannell L K, Waugh D S, Byrd R A Protein expression and purification. Expression, purification, refolding, and characterization of recombinant human interleukin-13: utilization of intracellular processing. 2000 Nov: 20(2) 186-95 https://www.ncbi.nlm.nih.gov/pubmed/11049743

869 Boesen C C, Motyka S A, Patamawenu A, Sun P D Protein expression and purification. Development of a recombinant bacterial expression system for the active form of a human transforming growth factor beta type II receptor ligand binding domain. 2000 Oct: 20(1) 98-104 https://www.ncbi.nlm.nih.gov/pubmed/11035957

870 Conlan S, Zhang Y, Cheley S, Bayley H Biochemistry. Biochemical and biophysical characterization of OmpG: A monomeric porin. 2000 Oct: 39(39) 11845-54 https://www.ncbi.nlm.nih.gov/pubmed/11009596

871 De Guzman R N, Liu H Y, Martinez-Yamout M, Dyson H J, Wright P E Journal of molecular biology. Solution structure of the TAZ2 (CH3) domain of the transcriptional adaptor protein CBP. 2000 Oct: 303(2) 243-53 https://www.ncbi.nlm.nih.gov/pubmed/11023789

872 Scrofani S D, Fabri L J, Xu P, Maccarone P, Nash A D Protein science : a publication of the Protein Society. Purification and refolding of vascular endothelial growth factor-B. 2000 Oct: 9(10) 2018-25 https://www.ncbi.nlm.nih.gov/pubmed/11106176

873 Hrzenjak A, Frank S, Maderegger B, Sterk H, Kostner G M Protein engineering. Apo(a)-kringle IV-type 6: expression in Escherichia coli, purification and in vitro refolding. 2000 Sep: 13(9) 661-6 https://www.ncbi.nlm.nih.gov/pubmed/11054461

874 Yang S, Boroughs K L, McDermott M J Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. Canine interleukin-13: molecular cloning of full-length cDNA and expression of biologically active recombinant protein. 2000 Sep: 20(9) 779-85 https://www.ncbi.nlm.nih.gov/pubmed/11032397

875 Hung C C, Chiou S H Biochemical and biophysical research communications. Expression of a kallikrein-like protease from the snake venom: engineering of autocatalytic site in the fusion protein to facilitate protein refolding. 2000 Sep: 275(3) 924-30 https://www.ncbi.nlm.nih.gov/pubmed/10973823

876 Huang X P, Kagami N, Inoue H, Kojima M, Kimura T, Makabe O, Suzuki K, Takahashi K The Journal of biological chemistry. Identification of a glutamic acid and an aspartic acid residue essential for catalytic activity of aspergillopepsin II, a non-pepsin type acid proteinase. 2000 Aug: 275(34) 26607-14 https://www.ncbi.nlm.nih.gov/pubmed/10854444

877 Grauschopf U, Lilie H, Honold K, Wozny M, Reusch D, Esswein A, Schäfer W, Rücknagel K P, Rudolph R Biochemistry. The N-terminal fragment of human parathyroid hormone receptor 1 constitutes a hormone binding domain and reveals a distinct disulfide pattern. 2000 Aug: 39(30) 8878-87 https://www.ncbi.nlm.nih.gov/pubmed/10913300

878 Hsieh M C, Cooperman B S Biochimica et biophysica acta. The preparation and catalytic properties of recombinant human prostate-specific antigen (rPSA). 2000 Aug: 1481(1) 75-87 https://www.ncbi.nlm.nih.gov/pubmed/10962094

879 Korn C, Meiss G, Gast F, Gimadutdinow O, Urbanke C, Pingoud A Gene. Genetic engineering of Escherichia coli to produce a 1:1 complex of the anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA. 2000 Aug: 253(2) 221-9 https://www.ncbi.nlm.nih.gov/pubmed/10940560

880 Baldermann C, Engelhardt H Journal of structural biology. Expression, two-dimensional crystallization, and three-dimensional reconstruction of the beta8 outer membrane protein Omp21 from Comamonas acidovorans. 2000 Aug: 131(2) 96-107 https://www.ncbi.nlm.nih.gov/pubmed/11042080

881 Park S G, Lee J S, Je E Y, Kim I J, Chung J H, Choi I H Biochemical and biophysical research communications. Affinity maturation of natural antibody using a chain shuffling technique and the expression of recombinant antibodies in Escherichia coli. 2000 Aug: 275(2) 553-7 https://www.ncbi.nlm.nih.gov/pubmed/10964702

882 García-Sáez I, Plasterk R H Protein expression and purification. Purification of the Caenorhabditis elegans transposase Tc1A refolded during gel filtration chromatography. 2000 Aug: 19(3) 355-61 https://www.ncbi.nlm.nih.gov/pubmed/10910725

883 Hardern I M, Knauper V, Ernill R J, Taylor I W, Cooper K L, Abbott W M Protein expression and purification. An analysis of two refolding routes for a C-terminally truncated human collagenase-3 expressed in Escherichia coli. 2000 Jul: 19(2) 246-52 https://www.ncbi.nlm.nih.gov/pubmed/10873537

884 Hatters D M, MacPhee C E, Lawrence L J, Sawyer W H, Howlett G J Biochemistry. Human apolipoprotein C-II forms twisted amyloid ribbons and closed loops. 2000 Jul: 39(28) 8276-83 https://www.ncbi.nlm.nih.gov/pubmed/10889036

885 López-Vara M C, Gasset M, Pajares M A Protein expression and purification. Refolding and characterization of rat liver methionine adenosyltransferase from Escherichia coli inclusion bodies. 2000 Jul: 19(2) 219-26 https://www.ncbi.nlm.nih.gov/pubmed/10873534

886 Raver N, Gussakovsky E E, Keisler D H, Krishna R, Mistry J, Gertler A Protein expression and purification. Preparation of recombinant bovine, porcine, and porcine W4R/R5K leptins and comparison of their activity and immunoreactivity with ovine, chicken, and human leptins. 2000 Jun: 19(1) 30-40 https://www.ncbi.nlm.nih.gov/pubmed/10833387

887 Babu K R, Swaminathan S, Marten S, Khanna N, Rinas U Applied microbiology and biotechnology. Production of interferon-alpha in high cell density cultures of recombinant Escherichia coli and its single step purification from refolded inclusion body proteins. 2000 Jun: 53(6) 655-60 https://www.ncbi.nlm.nih.gov/pubmed/10919322

888 Askarian-Amiri M E, Pel H J, Guévremont D, McCaughan K K, Poole E S, Sumpter V G, Tate W P The Journal of biological chemistry. Functional characterization of yeast mitochondrial release factor 1. 2000 Jun: 275(23) 17241-8 https://www.ncbi.nlm.nih.gov/pubmed/10748224

889 Takahashi K, Takai T, Yasuhara T, Yuuki T, Ohtake Y, Yokota T, Okumura Y International archives of allergy and immunology. Production of enzymatically and immunologically active Der f 1 in Escherichia coli. 2000 Jun: 122(2) 108-14 https://www.ncbi.nlm.nih.gov/pubmed/10878489

890 Lee J Y, Yoon C S, Chung I Y, Lee Y S, Lee E K Biotechnology and applied biochemistry. Scale-up process for expression and renaturation of recombinant human epidermal growth factor from Escherichia coli inclusion bodies. 2000 Jun: 31 ( Pt 3) 245-8 https://www.ncbi.nlm.nih.gov/pubmed/10814596

891 Faure M, Bourguignon J, Neuburger M, MacHerel D, Sieker L, Ober R, Kahn R, Cohen-Addad C, Douce R European journal of biochemistry / FEBS. Interaction between the lipoamide-containing H-protein and the lipoamide dehydrogenase (L-protein) of the glycine decarboxylase multienzyme system 2. Crystal structures of H- and L-proteins. 2000 May: 267(10) 2890-8 https://www.ncbi.nlm.nih.gov/pubmed/10806386

892 Linardou H, Epenetos A A, Deonarain M P International journal of cancer. A recombinant cytotoxic chimera based on mammalian deoxyribonuclease-I. 2000 May: Journal international du cancer. 86(4) 561-9 https://www.ncbi.nlm.nih.gov/pubmed/10797272

893 Stracke J O, Hutton M, Stewart M, Pendás A M, Smith B, López-Otin C, Murphy G, Knäuper V The Journal of biological chemistry. Biochemical characterization of the catalytic domain of human matrix metalloproteinase 19. Evidence for a role as a potent basement membrane degrading enzyme. 2000 May: 275(20) 14809-16 https://www.ncbi.nlm.nih.gov/pubmed/10809722

894 Booth C L, Pulaski L, Gottesman M M, Pastan I Biochemistry. Analysis of the properties of the N-terminal nucleotide-binding domain of human P-glycoprotein. 2000 May: 39(18) 5518-26 https://www.ncbi.nlm.nih.gov/pubmed/10820025

895 Amada K, Haruki M, Imanaka T, Morikawa M, Kanaya S Biochimica et biophysica acta. Overproduction in Escherichia coli, purification and characterization of a family I.3 lipase from Pseudomonas sp. MIS38. 2000 May: 1478(2) 201-10 https://www.ncbi.nlm.nih.gov/pubmed/10825531

896 Sandowski Y, Cohen Y, Le Rouzic P, Bignon C, Rentier-Delrue F, Djiane J, Prunet P, Gertler A General and comparative endocrinology. Recombinant prolactin receptor extracellular domain of rainbow trout (Oncorhynchus mykiss): subcloning, preparation, and characterization. 2000 May: 118(2) 302-9 https://www.ncbi.nlm.nih.gov/pubmed/10890569

897 McDermott M J, Weber E, Hunter S, Stedman K E, Best E, Frank G R, Wang R, Escudero J, Kuner J, McCall C Molecular immunology. Identification, cloning, and characterization of a major cat flea salivary allergen (Cte f 1). 2000 May: 37(7) 361-75 https://www.ncbi.nlm.nih.gov/pubmed/11074254

898 Im H, Yu M H Protein science : a publication of the Protein Society. Role of Lys335 in the metastability and function of inhibitory serpins. 2000 May: 9(5) 934-41 https://www.ncbi.nlm.nih.gov/pubmed/10850803

899 Assadi-Porter F M, Aceti D J, Cheng H, Markley J L Archives of biochemistry and biophysics. Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin. 2000 Apr: 376(2) 252-8 https://www.ncbi.nlm.nih.gov/pubmed/10775410

900 Maiorano D, Moreau J, Méchali M Nature. XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis. 2000 Apr: 404(6778) 622-5 https://www.ncbi.nlm.nih.gov/pubmed/10766247

901 Sanderson S J, Pollock K G, Hilley J D, Meldal M, Hilaire P S, Juliano M A, Juliano L, Mottram J C, Coombs G H The Biochemical journal. Expression and characterization of a recombinant cysteine proteinase of Leishmania mexicana. 2000 Apr: 347(Pt 2) 383-8 https://www.ncbi.nlm.nih.gov/pubmed/10749667

902 Sunitha K, Chung B H, Jang K H, Song K B, Kim C H, Rhee S K Protein expression and purification. Refolding and purification of Zymomonas mobilis levansucrase produced as inclusion bodies in fed-batch culture of recombinant Escherichia coli. 2000 Apr: 18(3) 388-93 https://www.ncbi.nlm.nih.gov/pubmed/10733894

903 Margetts M B, Barr I G, Webb E A Protein expression and purification. Overexpression, purification, and refolding of a Porphyromonas gingivalis cysteine protease from Escherichia coli. 2000 Apr: 18(3) 262-8 https://www.ncbi.nlm.nih.gov/pubmed/10733878

904 Minami Y, Kawasaki H, Minami M, Tanahashi N, Tanaka K, Yahara I The Journal of biological chemistry. A critical role for the proteasome activator PA28 in the Hsp90-dependent protein refolding. 2000 Mar: 275(12) 9055-61 https://www.ncbi.nlm.nih.gov/pubmed/10722756

905 Bieger B, Essen L O Acta crystallographica. Crystallization and preliminary X-ray analysis of the catalytic domain of the adenylate cyclase GRESAG4.1 from Trypanosoma brucei. 2000 Mar: Section D, Biological crystallography. 56(Pt 3) 359-62 https://www.ncbi.nlm.nih.gov/pubmed/10713527

906 Boyington J C, Raiz A N, Brooks A G, Patamawenu A, Sun P D Protein expression and purification. Reconstitution of bacterial expressed human CD94: the importance of the stem region for dimer formation. 2000 Mar: 18(2) 235-41 https://www.ncbi.nlm.nih.gov/pubmed/10686155

907 Patra A K, Mukhopadhyay R, Mukhija R, Krishnan A, Garg L C, Panda A K Protein expression and purification. Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli. 2000 Mar: 18(2) 182-92 https://www.ncbi.nlm.nih.gov/pubmed/10686149

908 Ceciliani F, Caramori T, Ronchi S, Tedeschi G, Mortarino M, Galizzi A Protein expression and purification. Cloning, overexpression, and purification of Escherichia coli quinolinate synthetase. 2000 Feb: 18(1) 64-70 https://www.ncbi.nlm.nih.gov/pubmed/10648170

909 Remmert K, Vullhorst D, Hinssen H Protein expression and purification. In vitro refolding of heterodimeric CapZ expressed in E. coli as inclusion body protein. 2000 Feb: 18(1) 11-9 https://www.ncbi.nlm.nih.gov/pubmed/10648164

910 Arora A, Rinehart D, Szabo G, Tamm L K The Journal of biological chemistry. Refolded outer membrane protein A of Escherichia coli forms ion channels with two conductance states in planar lipid bilayers. 2000 Jan: 275(3) 1594-600 https://www.ncbi.nlm.nih.gov/pubmed/10636850

911 Honda J, Andou H, Mannen T, Sugimoto S Journal of bioscience and bioengineering. Direct refolding of recombinant human growth differentiation factor 5 for large-scale production process. 2000 : 89(6) 582-9 https://www.ncbi.nlm.nih.gov/pubmed/16232802

912 Fahey E M, Chaudhuri J B, Binding P Journal of chromatography. Refolding and purification of a urokinase plasminogen activator fragment by chromatography. 2000 Jan: B, Biomedical sciences and applications. 737(1-2) 225-35 https://www.ncbi.nlm.nih.gov/pubmed/10681059

913 Thies M J, Pirkl F Journal of chromatography. Chromatographic purification of the C(H)2 domain of the monoclonal antibody MAK33. 2000 Jan: B, Biomedical sciences and applications. 737(1-2) 63-9 https://www.ncbi.nlm.nih.gov/pubmed/10681042

914 Linden A, Niehaus F, Antranikian G Journal of chromatography. Single-step purification of a recombinant thermostable alpha-amylase after solubilization of the enzyme from insoluble aggregates. 2000 Jan: B, Biomedical sciences and applications. 737(1-2) 253-9 https://www.ncbi.nlm.nih.gov/pubmed/10681062

915 Majerle A, Kidric J, Jerala R Pflügers Archiv : European journal of physiology. Bacterial expression and refolding of different fragments of human CD14. 2000 : 439(3 Suppl) R109-10 https://www.ncbi.nlm.nih.gov/pubmed/10653159

916 Jacquet A, Daminet V, Haumont M, Garcia L, Chaudoir S, Bollen A, Biemans R Protein expression and purification. Expression of a recombinant Toxoplasma gondii ROP2 fragment as a fusion protein in bacteria circumvents insolubility and proteolytic degradation. 1999 Dec: 17(3) 392-400 https://www.ncbi.nlm.nih.gov/pubmed/10600457

917 Buchanan S K Biochemical Society transactions. Overexpression and refolding of an 80-kDa iron transporter from the outer membrane of Escherichia coli. 1999 Dec: 27(6) 903-8 https://www.ncbi.nlm.nih.gov/pubmed/10830125

918 Kahler A E, Nielsen F S, Switzer R L Archives of biochemistry and biophysics. Biochemical characterization of the heteromeric Bacillus subtilis dihydroorotate dehydrogenase and its isolated subunits. 1999 Nov: 371(2) 191-201 https://www.ncbi.nlm.nih.gov/pubmed/10545205

919 Yamamoto Y, Watabe S, Kageyama T, Takahashi S Y Archives of insect biochemistry and physiology. Proregion of Bombyx mori cysteine proteinase functions as an intramolecular chaperone to promote proper folding of the mature enzyme. 1999 Nov: 42(3) 167-78 https://www.ncbi.nlm.nih.gov/pubmed/10536045

920 Yamamoto T, Shiraki K, Fujiwara S, Takagi M, Fukui K, Imanaka T Biochemical and biophysical research communications. In vitro heat effect on functional and conformational changes of cyclodextrin glucanotransferase from hyperthermophilic archaea. 1999 Nov: 265(1) 57-61 https://www.ncbi.nlm.nih.gov/pubmed/10548490

921 St John R J, Carpenter J F, Randolph T W Proceedings of the National Academy of Sciences of the United States of America. High pressure fosters protein refolding from aggregates at high concentrations. 1999 Nov: 96(23) 13029-33 https://www.ncbi.nlm.nih.gov/pubmed/10557267

922 Berdichevsky Y, Lamed R, Frenkel D, Gophna U, Bayer E A, Yaron S, Shoham Y, Benhar I Protein expression and purification. Matrix-assisted refolding of single-chain Fv- cellulose binding domain fusion proteins. 1999 Nov: 17(2) 249-59 https://www.ncbi.nlm.nih.gov/pubmed/10545273

923 Oneda H, Inouye K Journal of biochemistry. Refolding and recovery of recombinant human matrix metalloproteinase 7 (matrilysin) from inclusion bodies expressed by Escherichia coli. 1999 Nov: 126(5) 905-11 https://www.ncbi.nlm.nih.gov/pubmed/10544284

924 Canals A, Ribó M, Benito A, Bosch M, Mombelli E, Vilanova M Protein expression and purification. Production of engineered human pancreatic ribonucleases, solving expression and purification problems, and enhancing thermostability. 1999 Oct: 17(1) 169-81 https://www.ncbi.nlm.nih.gov/pubmed/10497083

925 Cox G N, Pratt D, Smith D, McDermott M J, Vanderslice R W Protein expression and purification. Refolding and characterization of recombinant human soluble CTLA-4 expressed in Escherichia coli. 1999 Oct: 17(1) 26-32 https://www.ncbi.nlm.nih.gov/pubmed/10497065

926 Boyle D M, McKinnie R E, Joy W D, Violand B N, McLaughlin J K, Landis B H Biotechnology and applied biochemistry. Evaluation of refolding conditions for a recombinant human interleukin-3 variant (daniplestim). 1999 Oct: 30 ( Pt 2) 163-70 https://www.ncbi.nlm.nih.gov/pubmed/10512796

927 Zouhar J, Nanak E, Brzobohatý B Protein expression and purification. Expression, single-step purification, and matrix-assisted refolding of a maize cytokinin glucoside-specific beta-glucosidase. 1999 Oct: 17(1) 153-62 https://www.ncbi.nlm.nih.gov/pubmed/10497081

928 Müller C, Rinas U Journal of chromatography. Renaturation of heterodimeric platelet-derived growth factor from inclusion bodies of recombinant Escherichia coli using size-exclusion chromatography. 1999 Sep: A. 855(1) 203-13 https://www.ncbi.nlm.nih.gov/pubmed/10514985

929 Wilharm E, Parry M A, Friebel R, Tschesche H, Matschiner G, Sommerhoff C P, Jenne D E The Journal of biological chemistry. Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma. 1999 Sep: 274(38) 27331-7 https://www.ncbi.nlm.nih.gov/pubmed/10480954

930 Gupta P, Waheed S M, Bhatnagar R Protein expression and purification. Expression and purification of the recombinant protective antigen of Bacillus anthracis. 1999 Aug: 16(3) 369-76 https://www.ncbi.nlm.nih.gov/pubmed/10425157

931 Meyer P, Janin J, Baudet-Nessler S European journal of biochemistry / FEBS. p55-hGRF, a short natural form of the Ras-GDP exchange factor high yield production and characterization. 1999 Aug: 263(3) 806-16 https://www.ncbi.nlm.nih.gov/pubmed/10469145

932 Maurice S, Hädge D, Dekel M, Friedman A, Gertler A, Shoseyov O Protein expression and purification. A-protein from achromogenic atypical Aeromonas salmonicida: molecular cloning, expression, purification, and characterization. 1999 Aug: 16(3) 396-404 https://www.ncbi.nlm.nih.gov/pubmed/10425160

933 Jeon O H, Kim D S European journal of biochemistry / FEBS. Molecular cloning and functional characterization of a snake venom metalloprotease. 1999 Jul: 263(2) 526-33 https://www.ncbi.nlm.nih.gov/pubmed/10406963

934 Pungercar J, Krizaj I, Liang N S, Gubensek F The Biochemical journal. An aromatic, but not a basic, residue is involved in the toxicity of group-II phospholipase A2 neurotoxins. 1999 Jul: 341 ( Pt 1) 139-45 https://www.ncbi.nlm.nih.gov/pubmed/10377255

935 Flentke G R, Glinski J, Satyshur K, Rich D H Protein expression and purification. Purification and crystallization of rhizopuspepsin: the use of nickel chelation chromatography to select for catalytically active species. 1999 Jul: 16(2) 213-20 https://www.ncbi.nlm.nih.gov/pubmed/10419816

936 Du Z, Gromet-Elhanan Z European journal of biochemistry / FEBS. Refolding of recombinant alpha and beta subunits of the Rhodospirillum rubrum F(0)F(1) ATP synthase into functional monomers that reconstitute an active alpha(1)beta(1)-dimer. 1999 Jul: 263(2) 430-7 https://www.ncbi.nlm.nih.gov/pubmed/10406951

937 Connaris H, Chaudhuri J B, Danson M J, Hough D W Biotechnology and bioengineering. Expression, reactivation, and purification of enzymes from Haloferax volcanii in Escherichia coli. 1999 Jul: 64(1) 38-45 https://www.ncbi.nlm.nih.gov/pubmed/10397837

938 Bera A K, Bernhardt R Archives of biochemistry and biophysics. GroEL-assisted and -unassisted refolding of mature and precursor adrenodoxin: the role of the precursor sequence. 1999 Jul: 367(1) 89-94 https://www.ncbi.nlm.nih.gov/pubmed/10375403

939 Zhao G, Meier T I, Hoskins J, Jaskunas S R Protein expression and purification. Penicillin-binding protein 2a of Streptococcus pneumoniae: expression in Escherichia coli and purification and refolding of inclusion bodies into a soluble and enzymatically active enzyme. 1999 Jul: 16(2) 331-9 https://www.ncbi.nlm.nih.gov/pubmed/10419829

940 Tran-Moseman A, Schauer N, De Bernardez Clark E Protein expression and purification. Renaturation of Escherichia coli-derived recombinant human macrophage colony-stimulating factor. 1999 Jun: 16(1) 181-9 https://www.ncbi.nlm.nih.gov/pubmed/10336876

941 Mossakowska D, Dodd I, Pindar W, Smith R A European journal of immunology. Structure-activity relationships within the N-terminal short consensus repeats (SCR) of human CR1 (C3b/C4b receptor, CD35): SCR 3 plays a critical role in inhibition of the classical and alternative pathways of complement activation. 1999 Jun: 29(6) 1955-65 https://www.ncbi.nlm.nih.gov/pubmed/10382758

942 Moellering B J, Yoshinaga S K, Hui A, Delaney J M, Hara S, Narhi L O, Westcott K R Protein expression and purification. Folding and purification of a recombinantly expressed interferon regulatory factor, IRF-4. 1999 Jun: 16(1) 160-70 https://www.ncbi.nlm.nih.gov/pubmed/10336874

943 Chen F E, Kempiak S, Huang D B, Phelps C, Ghosh G Protein engineering. Construction, expression, purification and functional analysis of recombinant NFkappaB p50/p65 heterodimer. 1999 May: 12(5) 423-8 https://www.ncbi.nlm.nih.gov/pubmed/10360983

944 Koshiba T, Hayashi T, Miwako I, Kumagai I, Ikura T, Kawano K, Nitta K, Kuwajima K Protein engineering. Expression of a synthetic gene encoding canine milk lysozyme in Escherichia coli and characterization of the expressed protein. 1999 May: 12(5) 429-35 https://www.ncbi.nlm.nih.gov/pubmed/10360984

945 Liu X, Pan H, Yang G, Wu X, Zhou Y Biochimica et biophysica acta. Cloning, expression and biochemical characterization of a basic-acidic hybrid phospholipase A2-II from Agkistrodon halys pallas. 1999 Apr: 1431(1) 157-65 https://www.ncbi.nlm.nih.gov/pubmed/10209288

946 Allan G J, Shand J H, Beattie J, Flint D J European journal of biochemistry / FEBS. Identification of novel sites in the ovine growth hormone receptor involved in binding hormone and conferring species specificity. 1999 Apr: 261(2) 555-62 https://www.ncbi.nlm.nih.gov/pubmed/10215869

947 Harrison S J, McManus A M, Marcus J P, Goulter K C, Green J L, Nielsen K J, Craik D J, Maclean D J, Manners J M Protein expression and purification. Purification and characterization of a plant antimicrobial peptide expressed in Escherichia coli. 1999 Mar: 15(2) 171-7 https://www.ncbi.nlm.nih.gov/pubmed/10049672

948 Lullien-Pellerin V, Devaux C, Ihorai T, Marion D, Pahin V, Joudrier P, Gautier M F European journal of biochemistry / FEBS. Production in Escherichia coli and site-directed mutagenesis of a 9-kDa nonspecific lipid transfer protein from wheat. 1999 Mar: 260(3) 861-8 https://www.ncbi.nlm.nih.gov/pubmed/10103017

949 Chen Z Y, Brown R L, Lax A R, Cleveland T E, Russin J S Applied and environmental microbiology. Inhibition of plant-pathogenic fungi by a corn trypsin inhibitor overexpressed in Escherichia coli. 1999 Mar: 65(3) 1320-4 https://www.ncbi.nlm.nih.gov/pubmed/10049901

950 D'alessio K J, McQueney M S, Brun K A, Orsini M J, Debouck C M Protein expression and purification. Expression in Escherichia coli, refolding, and purification of human procathepsin K, an osteoclast-specific protease. 1999 Mar: 15(2) 213-20 https://www.ncbi.nlm.nih.gov/pubmed/10049678

951 Anderson M, Blowers D, Hewitt N, Hedge P, Breeze A, Hampton I, Taylor I Protein expression and purification. Refolding, purification, and characterization of a loop deletion mutant of human Bcl-2 from bacterial inclusion bodies. 1999 Mar: 15(2) 162-70 https://www.ncbi.nlm.nih.gov/pubmed/10049671

952 Kati W M, Sham H L, McCall J O, Montgomery D A, Wang G T, Rosenbrook W, Miesbauer L, Buko A, Norbeck D W Archives of biochemistry and biophysics. Inhibition of 3C protease from human rhinovirus strain 1B by peptidyl bromomethylketonehydrazides. 1999 Feb: 362(2) 363-75 https://www.ncbi.nlm.nih.gov/pubmed/9989947

953 Mizoue L S, Bazan J F, Johnson E C, Handel T M Biochemistry. Solution structure and dynamics of the CX3C chemokine domain of fractalkine and its interaction with an N-terminal fragment of CX3CR1. 1999 Feb: 38(5) 1402-14 https://www.ncbi.nlm.nih.gov/pubmed/9931005

954 Pan H, Du X, Yang G, Zhou Y, Wu X Biochemical and biophysical research communications. cDNA cloning and expression of acutin. 1999 Feb: 255(2) 412-5 https://www.ncbi.nlm.nih.gov/pubmed/10049722

955 Kashima T, Morishita A, Iwata H, Maeda K, Inoue T The Journal of veterinary medical science / the Japanese Society of Veterinary Science. Expression of bovine cytokines in Escherichia coli. 1999 Feb: 61(2) 171-3 https://www.ncbi.nlm.nih.gov/pubmed/10081758

956 Ejima D, Watanabe M, Sato Y, Date M, Yamada N, Takahara Y Biotechnology and bioengineering. High yield refolding and purification process for recombinant human interleukin-6 expressed in Escherichia coli. 1999 Feb: 62(3) 301-10 https://www.ncbi.nlm.nih.gov/pubmed/10099541

957 Pautsch A, Vogt J, Model K, Siebold C, Schulz G E Proteins. Strategy for membrane protein crystallization exemplified with OmpA and OmpX. 1999 Feb: 34(2) 167-72 https://www.ncbi.nlm.nih.gov/pubmed/10022352

958 Altamirano M M, García C, Possani L D, Fersht A R Nature biotechnology. Oxidative refolding chromatography: folding of the scorpion toxin Cn5. 1999 Feb: 17(2) 187-91 https://www.ncbi.nlm.nih.gov/pubmed/10052357

959 Li N, Qu L J, Liu Y, Li Q, Gu H, Chen Z Protein expression and purification. The refolding, purification, and activity analysis of a rice Bowman-Birk inhibitor expressed in Escherichia coli. 1999 Feb: 15(1) 99-104 https://www.ncbi.nlm.nih.gov/pubmed/10024476

960 Bürgin J, Schaller J Cellular and molecular life sciences : CMLS. Expression, isolation and characterization of a mutated human plasminogen kringle 3 with a functional lysine binding site. 1999 Jan: 55(1) 135-41 https://www.ncbi.nlm.nih.gov/pubmed/10065160

961 Ben-Atia I, Fine M, Tandler A, Funkenstein B, Maurice S, Cavari B, Gertler A General and comparative endocrinology. Preparation of recombinant gilthead seabream (Sparus aurata) growth hormone and its use for stimulation of larvae growth by oral administration. 1999 Jan: 113(1) 155-64 https://www.ncbi.nlm.nih.gov/pubmed/9882554

962 Tokuyasu K, Ohnishi-Kameyama M, Hayashi K, Mori Y Journal of bioscience and bioengineering. Cloning and expression of chitin deacetylase gene from a Deuteromycete, Colletotrichum lindemuthianum. 1999 : 87(4) 418-23 https://www.ncbi.nlm.nih.gov/pubmed/16232493

963 Gibson L C, Jensen P E, Hunter C N The Biochemical journal. Magnesium chelatase from Rhodobacter sphaeroides: initial characterization of the enzyme using purified subunits and evidence for a BchI-BchD complex. 1999 Jan: 337 ( Pt 2) 243-51 https://www.ncbi.nlm.nih.gov/pubmed/9882621

964 Daugherty D L, Rozema D, Hanson P E, Gellman S H The Journal of biological chemistry. Artificial chaperone-assisted refolding of citrate synthase. 1998 Dec: 273(51) 33961-71 https://www.ncbi.nlm.nih.gov/pubmed/9852049

965 Chen G Q, Sun Y, Jin R, Gouaux E Protein science : a publication of the Protein Society. Probing the ligand binding domain of the GluR2 receptor by proteolysis and deletion mutagenesis defines domain boundaries and yields a crystallizable construct. 1998 Dec: 7(12) 2623-30 https://www.ncbi.nlm.nih.gov/pubmed/9865957

966 Tomasselli A G, Paddock D J, Curry K A, Garlick R L, Leone J W, Lull J M, Mutchler V T, Baker C A, Cavey G S, Mathews W R, Shelly J A, Finzel B C, Baldwin E T, Wells P A, Tomich C S Protein expression and purification. Recombinant human cytomegalovirus protease with a C-terminal (His)6 extension: purification, autocatalytic release of the mature enzyme, and biochemical characterization. 1998 Dec: 14(3) 343-52 https://www.ncbi.nlm.nih.gov/pubmed/9882568

967 Raver N, Taouis M, Dridi S, Derouet M, Simon J, Robinzon B, Djiane J, Gertler A Protein expression and purification. Large-scale preparation of biologically active recombinant chicken obese protein (leptin). 1998 Dec: 14(3) 403-8 https://www.ncbi.nlm.nih.gov/pubmed/9882575

968 Ding Y S, Rich D H, Ikeda R A Biochemistry. Substrates and inhibitors of human T-cell leukemia virus type I protease. 1998 Dec: 37(50) 17514-8 https://www.ncbi.nlm.nih.gov/pubmed/9860866

969 Bottomley S P, Stone S R Protein engineering. Protein engineering of chimeric Serpins: an investigation into effects of the serpin scaffold and reactive centre loop length. 1998 Dec: 11(12) 1243-7 https://www.ncbi.nlm.nih.gov/pubmed/9930674

970 Gerhartz B, Ekiel I, Abrahamson M Biochemistry. Two stable unfolding intermediates of the disease-causing L68Q variant of human cystatin C. 1998 Dec: 37(49) 17309-17 https://www.ncbi.nlm.nih.gov/pubmed/9860845

971 Schrattenholz A, Pfeiffer S, Pejovic V, Rudolph R, Godovac-Zimmermann J, Maelicke A The Journal of biological chemistry. Expression and renaturation of the N-terminal extracellular domain of torpedo nicotinic acetylcholine receptor alpha-subunit. 1998 Dec: 273(49) 32393-9 https://www.ncbi.nlm.nih.gov/pubmed/9829968

972 Varnerin J P, Smith T, Rosenblum C I, Vongs A, Murphy B A, Nunes C, Mellin T N, King J J, Burgess B W, Junker B, Chou M, Hey P, Frazier E, MacIntyre D E, Van der Ploeg L H, Tota M R Protein expression and purification. Production of leptin in Escherichia coli: a comparison of methods. 1998 Dec: 14(3) 335-42 https://www.ncbi.nlm.nih.gov/pubmed/9882567

973 Inoue M, Akimaru J, Nishikawa T, Seki N, Yamada H Biotechnology and applied biochemistry. A new derivatizing agent, trimethylammoniopropyl methanethiosulphonate, is efficient for preparation of recombinant brain-derived neurotrophic factor from inclusion bodies. 1998 Dec: 28 ( Pt 3) 207-13 https://www.ncbi.nlm.nih.gov/pubmed/9799718

974 Buhler C, Gadelle D, Forterre P, Wang J C, Bergerat A Nucleic acids research. Reconstitution of DNA topoisomerase VI of the thermophilic archaeon Sulfolobus shibatae from subunits separately overexpressed in Escherichia coli. 1998 Nov: 26(22) 5157-62 https://www.ncbi.nlm.nih.gov/pubmed/9801313

975 Berthet F X, Rasmussen P B, Rosenkrands I, Andersen P, Gicquel B Microbiology (Reading, England). A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10). 1998 Nov: 144 ( Pt 11) 3195-203 https://www.ncbi.nlm.nih.gov/pubmed/9846755

976 Pathak N, Hu S I, Koehn J A Protein expression and purification. The expression, refolding, and purification of the catalytic domain of human collagenase-3 (MMP-13). 1998 Nov: 14(2) 283-8 https://www.ncbi.nlm.nih.gov/pubmed/9790892

977 Kakeda K, Jordan N D, Conner A, Ride J P, Franklin-Tong V E, Franklin F C The Plant cell. Identification of residues in a hydrophilic loop of the Papaver rhoeas S protein that play a crucial role in recognition of incompatible pollen. 1998 Oct: 10(10) 1723-32 https://www.ncbi.nlm.nih.gov/pubmed/9761798

978 Groppe J, Rumpel K, Economides A N, Stahl N, Sebald W, Affolter M The Journal of biological chemistry. Biochemical and biophysical characterization of refolded Drosophila DPP, a homolog of bone morphogenetic proteins 2 and 4. 1998 Oct: 273(44) 29052-65 https://www.ncbi.nlm.nih.gov/pubmed/9786911

979 Ambert-Balay K, Fuchs S M, Tien M Biochemical and biophysical research communications. Identification of the veratryl alcohol binding site in lignin peroxidase by site-directed mutagenesis. 1998 Oct: 251(1) 283-6 https://www.ncbi.nlm.nih.gov/pubmed/9790947

980 Goldbaum F A, Polikarpov I, Cauerhff A A, Velikovsky C A, Braden B C, Poljak R J Journal of structural biology. Crystallization and preliminary x-ray diffraction analysis of the lumazine synthase from Brucella abortus. 1998 Oct: 123(2) 175-8 https://www.ncbi.nlm.nih.gov/pubmed/9843672

981 Tsumoto K, Shinoki K, Kondo H, Uchikawa M, Juji T, Kumagai I Journal of immunological methods. Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment. 1998 Oct: 219(1-2) 119-29 https://www.ncbi.nlm.nih.gov/pubmed/9831393

982 Xie Y, Lashuel H A, Miroy G J, Dikler S, Kelly J W Protein expression and purification. Recombinant human retinol-binding protein refolding, native disulfide formation, and characterization. 1998 Oct: 14(1) 31-7 https://www.ncbi.nlm.nih.gov/pubmed/9758748

983 Suenaga M, Ohmae H, Tsuji S, Itoh T, Nishimura O Biotechnology and applied biochemistry. Renaturation of recombinant human neurotrophin-3 from inclusion bodies using a suppressor agent of aggregation. 1998 Oct: 28 ( Pt 2) 119-24 https://www.ncbi.nlm.nih.gov/pubmed/9756741

984 Kataoka K, Nakai M, Yamaguchi K, Suzuki S Biochemical and biophysical research communications. Gene synthesis, expression, and mutagenesis of zucchini mavicyanin: the fourth ligand of blue copper center is Gln. 1998 Sep: 250(2) 409-13 https://www.ncbi.nlm.nih.gov/pubmed/9753643

985 Stone D B, Timmins P A, Schneider D K, Krylova I, Ramos C H, Reinach F C, Mendelson R A Journal of molecular biology. The effect of regulatory Ca2+ on the in situ structures of troponin C and troponin I: a neutron scattering study. 1998 Aug: 281(4) 689-704 https://www.ncbi.nlm.nih.gov/pubmed/9710540

986 Indiveri C, Iacobazzi V, Giangregorio N, Palmieri F Biochemical and biophysical research communications. Bacterial overexpression, purification, and reconstitution of the carnitine/acylcarnitine carrier from rat liver mitochondria. 1998 Aug: 249(3) 589-94 https://www.ncbi.nlm.nih.gov/pubmed/9731180

987 Fiermonte G, Dolce V, Palmieri F The Journal of biological chemistry. Expression in Escherichia coli, functional characterization, and tissue distribution of isoforms A and B of the phosphate carrier from bovine mitochondria. 1998 Aug: 273(35) 22782-7 https://www.ncbi.nlm.nih.gov/pubmed/9712911

988 Beer H D, McCarthy J E, Bornscheuer U T, Schmid R D Biochimica et biophysica acta. Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae. 1998 Aug: 1399(2-3) 173-80 https://www.ncbi.nlm.nih.gov/pubmed/9765593

989 Bera T K, Onda M, Brinkmann U, Pastan I Journal of molecular biology. A bivalent disulfide-stabilized Fv with improved antigen binding to erbB2. 1998 Aug: 281(3) 475-83 https://www.ncbi.nlm.nih.gov/pubmed/9698563

990 Park H U, Lee K J Microbiology (Reading, England). Cloning and heterologous expression of the gene for BLIP-II, a beta-lactamase-inhibitory protein from Streptomyces exfoliatus SMF19. 1998 Aug: 144 ( Pt 8) 2161-7 https://www.ncbi.nlm.nih.gov/pubmed/9720037

991 Ching W M, Wang H, Eamsila C, Kelly D J, Dasch G A Clinical and diagnostic laboratory immunology. Expression and refolding of truncated recombinant major outer membrane protein antigen (r56) of Orientia tsutsugamushi and its use in enzyme-linked immunosorbent assays. 1998 Jul: 5(4) 519-26 https://www.ncbi.nlm.nih.gov/pubmed/9665960

992 Meyer J D, Hanagan A, Manning M C, Catalano C E International journal of biological macromolecules. The phage lambda terminase enzyme: 1. Reconstitution of the holoenzyme from the individual subunits enhances the thermal stability of the small subunit. 1998 Jul: 23(1) 27-36 https://www.ncbi.nlm.nih.gov/pubmed/9644594

993 Laurence J S, LiWang A C, LiWang P J Biochemistry. Effect of N-terminal truncation and solution conditions on chemokine dimer stability: nuclear magnetic resonance structural analysis of macrophage inflammatory protein 1 beta mutants. 1998 Jun: 37(26) 9346-54 https://www.ncbi.nlm.nih.gov/pubmed/9649315

994 Helman D, Staten N R, Grosclaude J, Daniel N, Nespoulous C, Djiane J, Gertler A The Journal of biological chemistry. Novel recombinant analogues of bovine placental lactogen. G133K and G133R provide a tool to understand the difference between the action of prolactin and growth hormone receptors. 1998 Jun: 273(26) 16067-74 https://www.ncbi.nlm.nih.gov/pubmed/9632658

995 Hou J, Zhan H Cytokine. Expression of active thrombopoietin and identification of its key residues responsible for receptor binding. 1998 May: 10(5) 319-30 https://www.ncbi.nlm.nih.gov/pubmed/9619369

996 Labesse G, Craescu C T, Mispelter J, Chottard G, Marden M C, Pin S, Forest E, Mornon J P, Boccara M European journal of biochemistry / FEBS. Engineering, expression and biochemical characterization of the hemoglobin domain of a Erwinia chrysanthemi flavohemoprotein. 1998 May: 253(3) 751-9 https://www.ncbi.nlm.nih.gov/pubmed/9654075

997 Han S K, Yoon E T, Cho W The Biochemical journal. Bacterial expression and characterization of human secretory class V phospholipase A2. 1998 Apr: 331 ( Pt 2) 353-7 https://www.ncbi.nlm.nih.gov/pubmed/9531469

998 Chu V, Freitag S, Le Trong I, Stenkamp R E, Stayton P S Protein science : a publication of the Protein Society. Thermodynamic and structural consequences of flexible loop deletion by circular permutation in the streptavidin-biotin system. 1998 Apr: 7(4) 848-59 https://www.ncbi.nlm.nih.gov/pubmed/9568892

999 Almog O, Gallagher T, Tordova M, Hoskins J, Bryan P, Gilliland G L Proteins. Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain. 1998 Apr: 31(1) 21-32 https://www.ncbi.nlm.nih.gov/pubmed/9552156

1000 Li H, Zhang Y, Dong Y, Yang K Biochimica et biophysica acta. Functional implications of the 21-24 loop in recombinant prochymosin. 1998 Apr: 1384(1) 121-9 https://www.ncbi.nlm.nih.gov/pubmed/9602090

1001 Huxtable S, Zhou H, Wong S, Li N Protein expression and purification. Renaturation of 1-aminocyclopropane-1-carboxylate synthase expressed in Escherichia coli in the form of inclusion bodies into a dimeric and catalytically active enzyme. 1998 Apr: 12(3) 305-14 https://www.ncbi.nlm.nih.gov/pubmed/9535697

1002 Heim J, Schmidt-Dannert C, Atomi H, Schmid R D Biochimica et biophysica acta. Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli. 1998 Mar: 1396(3) 306-19 https://www.ncbi.nlm.nih.gov/pubmed/9545588

1003 Marie-Claire C, Roques B P, Beaumont A The Journal of biological chemistry. Intramolecular processing of prothermolysin. 1998 Mar: 273(10) 5697-701 https://www.ncbi.nlm.nih.gov/pubmed/9488701

1004 Song J, Minetti C A, Blake M S, Colombini M Biochimica et biophysica acta. Successful recovery of the normal electrophysiological properties of PorB (class 3) porin from Neisseria meningitidis after expression in Escherichia coli and renaturation. 1998 Mar: 1370(2) 289-98 https://www.ncbi.nlm.nih.gov/pubmed/9545584

1005 Klein H, Löschner B, Zyto N, Pörtner M, Montag T Glycoconjugate journal. Expression, purification, and biochemical characterization of a recombinant lectin of Sarcocystis muris (Apicomplexa) cyst merozoites. 1998 Feb: 15(2) 147-53 https://www.ncbi.nlm.nih.gov/pubmed/9557874

1006 Hurt J D, Tu C, Laipis P J Protein expression and purification. Isolation and expression of murine carbonic anhydrase IV. 1998 Feb: 12(1) 7-16 https://www.ncbi.nlm.nih.gov/pubmed/9473451

1007 Chang S G, Kim D Y, Choi K D, Shin J M, Shin H C The Biochemical journal. Human insulin production from a novel mini-proinsulin which has high receptor-binding activity. 1998 Feb: 329 ( Pt 3) 631-5 https://www.ncbi.nlm.nih.gov/pubmed/9445392

1008 Takeuchi A, Makino O, Hirokawa H Biochemical and biophysical research communications. Gene 1, one of the dna genes of bacteriophage phi 29, represses other dna genes through binding to mRNA. 1998 Feb: 243(2) 566-71 https://www.ncbi.nlm.nih.gov/pubmed/9480849

1009 Longstaff M, Powell K S, Gatehouse J A, Raemaekers R, Newell C A, Hamilton W D European journal of biochemistry / FEBS. Production and purification of active snowdrop lectin in Escherichia coli. 1998 Feb: 252(1) 59-65 https://www.ncbi.nlm.nih.gov/pubmed/9523712

1010 Sachdev D, Chirgwin J M Protein expression and purification. Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin. 1998 Feb: 12(1) 122-32 https://www.ncbi.nlm.nih.gov/pubmed/9473466

1011 Chapdelaine Y, Hohn T Virus genes. The cauliflower mosaic virus capsid protein: assembly and nucleic acid binding in vitro. 1998 : 17(2) 139-50 https://www.ncbi.nlm.nih.gov/pubmed/9857987

1012 Nalefski E A, McDonagh T, Somers W, Seehra J, Falke J J, Clark J D The Journal of biological chemistry. Independent folding and ligand specificity of the C2 calcium-dependent lipid binding domain of cytosolic phospholipase A2. 1998 Jan: 273(3) 1365-72 https://www.ncbi.nlm.nih.gov/pubmed/9430670

1013 Chitpinityol S, Goode D, Crabbe M J Molecular vision. Studies on the binding of alpha-crystallin to recombinant prochymosins and chymosin. 1998 Jan: 4 1 https://www.ncbi.nlm.nih.gov/pubmed/9485484

1014 Gertler A, Simmons J, Keisler D H FEBS letters. Large-scale preparation of biologically active recombinant ovine obese protein (leptin). 1998 Jan: 422(2) 137-40 https://www.ncbi.nlm.nih.gov/pubmed/9489992

1015 Chen G Q, Gouaux E Proceedings of the National Academy of Sciences of the United States of America. Overexpression of a glutamate receptor (GluR2) ligand binding domain in Escherichia coli: application of a novel protein folding screen. 1997 Dec: 94(25) 13431-6 https://www.ncbi.nlm.nih.gov/pubmed/9391042

1016 Nedelkov D, O'Keefe M P, Chapman T L, Bieber A L Biochemical and biophysical research communications. The role of Pro20 in the isomerization of myotoxin a from Crotalus viridis viridis: folding and structural characterization of synthetic myotoxin a and its Pro20Gly homolog. 1997 Dec: 241(2) 525-9 https://www.ncbi.nlm.nih.gov/pubmed/9425304

1017 Khandekar S S, Bettencourt B M, Wyss D F, Naylor J W, Brauer P P, Huestis K, Dwyer D S, Profy A T, Osburne M S, Banerji J, Jones B The Journal of biological chemistry. Conformational integrity and ligand binding properties of a single chain T-cell receptor expressed in Escherichia coli. 1997 Dec: 272(51) 32190-7 https://www.ncbi.nlm.nih.gov/pubmed/9405420

1018 Han B, Hall F L, Nimni M E Protein expression and purification. Refolding of a recombinant collagen-targeted TGF-beta2 fusion protein expressed in Escherichia coli. 1997 Nov: 11(2) 169-78 https://www.ncbi.nlm.nih.gov/pubmed/9367813

1019 Zahn R, von Schroetter C, Wüthrich K FEBS letters. Human prion proteins expressed in Escherichia coli and purified by high-affinity column refolding. 1997 Nov: 417(3) 400-4 https://www.ncbi.nlm.nih.gov/pubmed/9409760

1020 Liu W, Wang D, Chen P, Halpern M The Journal of biological chemistry. Cloning and expression of a gene encoding a protein obtained from earthworm secretion that is a chemoattractant for garter snakes. 1997 Oct: 272(43) 27378-81 https://www.ncbi.nlm.nih.gov/pubmed/9341189

1021 Simmons T, Newhouse Y M, Arnold K S, Innerarity T L, Weisgraber K H The Journal of biological chemistry. Human low density lipoprotein receptor fragment. Successful refolding of a functionally active ligand-binding domain produced in Escherichia coli. 1997 Oct: 272(41) 25531-6 https://www.ncbi.nlm.nih.gov/pubmed/9325268

1022 Mehta D V, DiGate R J, Banville D L, Guiles R D Protein expression and purification. Optimized gene synthesis, high level expression, isotopic enrichment, and refolding of human interleukin-5. 1997 Oct: 11(1) 86-94 https://www.ncbi.nlm.nih.gov/pubmed/9325143

1023 Kröger M, Tschesche H Gene. Cloning, expression and activation of a truncated 92-kDa gelatinase minienzyme. 1997 Sep: 196(1-2) 175-80 https://www.ncbi.nlm.nih.gov/pubmed/9322755

1024 Yang Y, Dignam J D, Gentry L E Biochemistry. Role of carbohydrate structures in the binding of beta1-latency-associated peptide to ligands. 1997 Sep: 36(39) 11923-32 https://www.ncbi.nlm.nih.gov/pubmed/9305986

1025 Marczinovits I, Somogyi C, Patthy A, Németh P, Molnár J Journal of biotechnology. An alternative purification protocol for producing hepatitis B virus X antigen on a preparative scale in Escherichia coli. 1997 Aug: 56(2) 81-8 https://www.ncbi.nlm.nih.gov/pubmed/9304871

1026 Tan C A, Hehir M J, Roberts M F Protein expression and purification. Cloning, overexpression, refolding, and purification of the nonspecific phospholipase C from Bacillus cereus. 1997 Aug: 10(3) 365-72 https://www.ncbi.nlm.nih.gov/pubmed/9268684

1027 Hsih M H, Kuo J C, Tsai H J Applied microbiology and biotechnology. Optimization of the solubilization and renaturation of fish growth hormone produced by Escherichia coli. 1997 Jul: 48(1) 66-72 https://www.ncbi.nlm.nih.gov/pubmed/9274049

1028 Braspenning J, Manetti R, Zumbach K, Meschede W, Gissmann L, Tommasino M Protein expression and purification. A general purification protocol for E7 proteins from "high- and low-risk" human papillomavirus types expressed in the yeast Schizosaccharomyces pombe. 1997 Jul: 10(2) 192-201 https://www.ncbi.nlm.nih.gov/pubmed/9226715

1029 Tchelet A, Vogel T, Helman D, Guy R, Neospouolus C, Goffin V, Djiane J, Gertler A Molecular and cellular endocrinology. Selective modification at the N-terminal region of human growth hormone that shows antagonistic activity. 1997 Jun: 130(1-2) 141-52 https://www.ncbi.nlm.nih.gov/pubmed/9220030

1030 Wakiyama M, Sakai N, Kojima S, Miura K FEBS letters. Disulfide bond formation is not involved in cap-binding activity of Xenopus translation initiation factor eIF-4E. 1997 Jun: 409(3) 407-10 https://www.ncbi.nlm.nih.gov/pubmed/9224699

1031 Boismenu R, Semeniuk D, Murgita R A Protein expression and purification. Purification and characterization of human and mouse recombinant alpha-fetoproteins expressed in Escherichia coli. 1997 Jun: 10(1) 10-26 https://www.ncbi.nlm.nih.gov/pubmed/9179285

1032 Gauthier M, Patston P A Analytical biochemistry. Reactivation of C1-inhibitor polymers by denaturation and gel-filtration chromatography. 1997 Jun: 248(2) 228-33 https://www.ncbi.nlm.nih.gov/pubmed/9177748

1033 Feng L, Prestwich G D Insect biochemistry and molecular biology. Expression and characterization of a lepidopteran general odorant binding protein. 1997 May: 27(5) 405-12 https://www.ncbi.nlm.nih.gov/pubmed/9219366

1034 Brinkmann U, Webber K, Di Carlo A, Beers R, Chowdhury P, Chang K, Chaudhary V, Gallo M, Pastan I International journal of cancer. Cloning and expression of the recombinant FAb fragment of monoclonal antibody K1 that reacts with mesothelin present on mesotheliomas and ovarian cancers. 1997 May: Journal international du cancer. 71(4) 638-44 https://www.ncbi.nlm.nih.gov/pubmed/9178820

1035 Ahn J H, Lee Y P, Rhee J S Journal of biotechnology. Investigation of refolding condition for Pseudomonas fluorescens lipase by response surface methodology. 1997 May: 54(3) 151-60 https://www.ncbi.nlm.nih.gov/pubmed/9208485

1036 Kahmann J D, Koruth R, Day A J Protein expression and purification. Method for quantitative refolding of the link module from human TSG-6. 1997 Apr: 9(3) 315-8 https://www.ncbi.nlm.nih.gov/pubmed/9126602

1037 Brown L R, Deng J, Noll D M, Mori N, Clarke N D Protein expression and purification. Construction and overexpression of a synthetic gene for human DNA methylguanine methyltransferase: renaturation and rapid purification of the protein. 1997 Apr: 9(3) 337-45 https://www.ncbi.nlm.nih.gov/pubmed/9126605

1038 Lohmeyer M, Harrison P M, Kannan S, DeSantis M, O'Reilly N J, Sternberg M J, Salomon D S, Gullick W J Biochemistry. Chemical synthesis, structural modeling, and biological activity of the epidermal growth factor-like domain of human cripto. 1997 Apr: 36(13) 3837-45 https://www.ncbi.nlm.nih.gov/pubmed/9092813

1039 Altamirano M M, Golbik R, Zahn R, Buckle A M, Fersht A R Proceedings of the National Academy of Sciences of the United States of America. Refolding chromatography with immobilized mini-chaperones. 1997 Apr: 94(8) 3576-8 https://www.ncbi.nlm.nih.gov/pubmed/9108018

1040 Liu H, Moreau J F, Gualde N, Fu J Molecular and cellular biochemistry. Refolding of soluble leukemia inhibitory factor receptor fusion protein (gp 190 sol DAF) from urea. 1997 Apr: 169(1-2) 43-50 https://www.ncbi.nlm.nih.gov/pubmed/9089630

1041 Andersen J F, Champagne D E, Weichsel A, Ribeiro J M, Balfour C A, Dress V, Montfort W R Biochemistry. Nitric oxide binding and crystallization of recombinant nitrophorin I, a nitric oxide transport protein from the blood-sucking bug Rhodnius prolixus. 1997 Apr: 36(15) 4423-8 https://www.ncbi.nlm.nih.gov/pubmed/9109649

1042 Boucher P E, Murakami K, Ishihama A, Stibitz S Journal of bacteriology. Nature of DNA binding and RNA polymerase interaction of the Bordetella pertussis BvgA transcriptional activator at the fha promoter. 1997 Mar: 179(5) 1755-63 https://www.ncbi.nlm.nih.gov/pubmed/9045838

1043 Gawlak S L, Neubauer M, Klei H E, Chang C Y, Einspahr H M, Siegall C B Biochemistry. Molecular, biological, and preliminary structural analysis of recombinant bryodin 1, a ribosome-inactivating protein from the plant Bryonia dioica. 1997 Mar: 36(11) 3095-103 https://www.ncbi.nlm.nih.gov/pubmed/9115985

1044 Kim Y T, Song Y H, Churchich J E Biochimica et biophysica acta. Recombinant brain 4-aminobutyrate aminotransferases overexpression, purification, and identification of Lys-330 at the active site. 1997 Feb: 1337(2) 248-56 https://www.ncbi.nlm.nih.gov/pubmed/9048902

1045 Saleh D, Furukawa K, Tsao M S, Maghazachi A, Corrin B, Yanagisawa M, Barnes P J, Giaid A American journal of respiratory cell and molecular biology. Elevated expression of endothelin-1 and endothelin-converting enzyme-1 in idiopathic pulmonary fibrosis: possible involvement of proinflammatory cytokines. 1997 Feb: 16(2) 187-93 https://www.ncbi.nlm.nih.gov/pubmed/9032126

1046 Pendás A M, Knäuper V, Puente X S, Llano E, Mattei M G, Apte S, Murphy G, López-Otín C The Journal of biological chemistry. Identification and characterization of a novel human matrix metalloproteinase with unique structural characteristics, chromosomal location, and tissue distribution. 1997 Feb: 272(7) 4281-6 https://www.ncbi.nlm.nih.gov/pubmed/9020145

1047 Cowley D J, Mackin R B FEBS letters. Expression, purification and characterization of recombinant human proinsulin. 1997 Feb: 402(2-3) 124-30 https://www.ncbi.nlm.nih.gov/pubmed/9037180

1048 Lemaître V, Jungbluth A, Eeckhout Y Biochemical and biophysical research communications. The recombinant catalytic domain of mouse collagenase-3 depolymerizes type I collagen by cleaving its aminotelopeptides. 1997 Jan: 230(1) 202-5 https://www.ncbi.nlm.nih.gov/pubmed/9020046

1049 Rozema D, Gellman S H Biochemistry. Artificial chaperone-assisted refolding of denatured-reduced lysozyme: modulation of the competition between renaturation and aggregation. 1996 Dec: 35(49) 15760-71 https://www.ncbi.nlm.nih.gov/pubmed/8961939

1050 Holloway D E, Harding S E, Marsh E N The Biochemical journal. Adenosylcobalamin-dependent glutamate mutase: properties of a fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit. 1996 Dec: 320 ( Pt 3) 825-30 https://www.ncbi.nlm.nih.gov/pubmed/9003368

1051 Arora D, Khanna N Journal of biotechnology. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. 1996 Dec: 52(2) 127-33 https://www.ncbi.nlm.nih.gov/pubmed/9084211

1052 Kiefer H, Krieger J, Olszewski J D, Von Heijne G, Prestwich G D, Breer H Biochemistry. Expression of an olfactory receptor in Escherichia coli: purification, reconstitution, and ligand binding. 1996 Dec: 35(50) 16077-84 https://www.ncbi.nlm.nih.gov/pubmed/8973178

1053 Garboczi D N, Utz U, Ghosh P, Seth A, Kim J, VanTienhoven E A, Biddison W E, Wiley D C Journal of immunology (Baltimore, Md. Assembly, specific binding, and crystallization of a human TCR-alphabeta with an antigenic Tax peptide from human T lymphotropic virus type 1 and the class I MHC molecule HLA-A2. 1996 Dec: : 1950). 157(12) 5403-10 https://www.ncbi.nlm.nih.gov/pubmed/8955188

1054 Hill A M, Cane D E, Mau C J, West C A Archives of biochemistry and biophysics. High level expression of Ricinus communis casbene synthase in Escherichia coli and characterization of the recombinant enzyme. 1996 Dec: 336(2) 283-9 https://www.ncbi.nlm.nih.gov/pubmed/8954576

1055 Engelman A Protein expression and purification. Biochemical characterization of recombinant equine infectious anemia virus integrase. 1996 Nov: 8(3) 299-304 https://www.ncbi.nlm.nih.gov/pubmed/8936591

1056 Gentil V, Martin P, Smal J, Le Bail P Y General and comparative endocrinology. Production of recombinant insulin-like growth factor-II in the development of a radioimmunoassay in rainbow trout (Oncorhynchus mykiss). 1996 Nov: 104(2) 156-67 https://www.ncbi.nlm.nih.gov/pubmed/8930606

1057 Christinger H W, Muller Y A, Berleau L T, Keyt B A, Cunningham B C, Ferrara N, de Vos A M Proteins. Crystallization of the receptor binding domain of vascular endothelial growth factor. 1996 Nov: 26(3) 353-7 https://www.ncbi.nlm.nih.gov/pubmed/8953654

1058 Furuya K, Hutchinson C R Journal of bacteriology. The DnrN protein of Streptomyces peucetius, a pseudo-response regulator, is a DNA-binding protein involved in the regulation of daunorubicin biosynthesis. 1996 Nov: 178(21) 6310-8 https://www.ncbi.nlm.nih.gov/pubmed/8892835

1059 Li Y, Inouye M Journal of molecular biology. The mechanism of autoprocessing of the propeptide of prosubtilisin E: intramolecular or intermolecular event? 1996 Oct: 262(5) 591-4 https://www.ncbi.nlm.nih.gov/pubmed/8876639

1060 Couthon F, Clottes E, Vial C Biochemical and biophysical research communications. Refolding of SDS- and thermally denatured MM-creatine kinase using cyclodextrins. 1996 Oct: 227(3) 854-60 https://www.ncbi.nlm.nih.gov/pubmed/8886021

1061 Dahan D, Srikumar R, Laprade R, Coulton J W FEBS letters. Purification and refolding of recombinant Haemophilus influenzae type b porin produced in Bacillus subtilis. 1996 Sep: 392(3) 304-8 https://www.ncbi.nlm.nih.gov/pubmed/8774868

1062 Boice J A, Fairman R Protein science : a publication of the Protein Society. Structural characterization of the tumor suppressor p16, an ankyrin-like repeat protein. 1996 Sep: 5(9) 1776-84 https://www.ncbi.nlm.nih.gov/pubmed/8880901

1063 Bassuk J A, Braun L P, Motamed K, Baneyx F, Sage E H The international journal of biochemistry & cell biology. Renaturation of SPARC expressed in Escherichia coli requires isomerization of disulfide bonds for recovery of biological activity. 1996 Sep: 28(9) 1031-43 https://www.ncbi.nlm.nih.gov/pubmed/8930126

1064 Talanian R V, Dang L C, Ferenz C R, Hackett M C, Mankovich J A, Welch J P, Wong W W, Brady K D The Journal of biological chemistry. Stability and oligomeric equilibria of refolded interleukin-1beta converting enzyme. 1996 Sep: 271(36) 21853-8 https://www.ncbi.nlm.nih.gov/pubmed/8702986

1065 Proudfoot A E, Goffin L, Payton M A, Wells T N, Bernard A R The Biochemical journal. In vivo and in vitro folding of a recombinant metalloenzyme, phosphomannose isomerase. 1996 Sep: 318 ( Pt 2) 437-42 https://www.ncbi.nlm.nih.gov/pubmed/8809030

1066 Hering T M, Kollar J, Huynh T D, Varelas J B Analytical biochemistry. Purification and characterization of decorin core protein expressed in Escherichia coli as a maltose-binding protein fusion. 1996 Aug: 240(1) 98-108 https://www.ncbi.nlm.nih.gov/pubmed/8811884

1067 Fan Q R, Garboczi D N, Winter C C, Wagtmann N, Long E O, Wiley D C Proceedings of the National Academy of Sciences of the United States of America. Direct binding of a soluble natural killer cell inhibitory receptor to a soluble human leukocyte antigen-Cw4 class I major histocompatibility complex molecule. 1996 Jul: 93(14) 7178-83 https://www.ncbi.nlm.nih.gov/pubmed/8692965

1068 Solovicová A, Gasperík J, Hostinová E Biochemical and biophysical research communications. High-yield production of Saccharomycopsis fibuligera glucoamylase in Escherichia coli, refolding, and comparison of the nonglycosylated and glycosylated enzyme forms. 1996 Jul: 224(3) 790-5 https://www.ncbi.nlm.nih.gov/pubmed/8713124

1069 Siemeister G, Schnurr B, Mohrs K, Schächtele C, Marmé D, Martiny-Baron G Biochemical and biophysical research communications. Expression of biologically active isoforms of the tumor angiogenesis factor VEGF in Escherichia coli. 1996 May: 222(2) 249-55 https://www.ncbi.nlm.nih.gov/pubmed/8670191

1070 Ernst S, Venkataraman G, Winkler S, Godavarti R, Langer R, Cooney C L, Sasisekharan R The Biochemical journal. Expression in Escherichia coli, purification and characterization of heparinase I from Flavobacterium heparinum. 1996 Apr: 315 ( Pt 2) 589-97 https://www.ncbi.nlm.nih.gov/pubmed/8615834

1071 Chang L S, Wu P F, Chang C C Biochemical and biophysical research communications. cDNA sequence analysis and expression of the a chain of beta-bungarotoxin from Bungarus multicinctus (Taiwan banded krait). 1996 Apr: 221(2) 328-32 https://www.ncbi.nlm.nih.gov/pubmed/8619855

1072 Betton J M, Hofnung M The Journal of biological chemistry. Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies. 1996 Apr: 271(14) 8046-52 https://www.ncbi.nlm.nih.gov/pubmed/8626487

1073 Doyle W A, Smith A T The Biochemical journal. Expression of lignin peroxidase H8 in Escherichia coli: folding and activation of the recombinant enzyme with Ca2+ and haem. 1996 Apr: 315 ( Pt 1) 15-9 https://www.ncbi.nlm.nih.gov/pubmed/8670100

1074 Hua Z C, Dong C, Zhu D X Biochemical and biophysical research communications. Renaturation of recombinant human pro-urokinase expressed in Escherichia coli. 1996 Mar: 220(1) 131-6 https://www.ncbi.nlm.nih.gov/pubmed/8602832

1075 Hill S, Austin S, Eydmann T, Jones T, Dixon R Proceedings of the National Academy of Sciences of the United States of America. Azotobacter vinelandii NIFL is a flavoprotein that modulates transcriptional activation of nitrogen-fixation genes via a redox-sensitive switch. 1996 Mar: 93(5) 2143-8 https://www.ncbi.nlm.nih.gov/pubmed/8700899

1076 Gross M, Wyss M, Furter-Graves E M, Wallimann T, Furter R Protein science : a publication of the Protein Society. Reconstitution of active octameric mitochondrial creatine kinase from two genetically engineered fragments. 1996 Feb: 5(2) 320-30 https://www.ncbi.nlm.nih.gov/pubmed/8745410

1077 Johnson D L, Middleton S A, McMahon F, Barbone F P, Kroon D, Tsao E, Lee W H, Mulcahy L S, Jolliffe L K Protein expression and purification. Refolding, purification, and characterization of human erythropoietin binding protein produced in Escherichia coli. 1996 Feb: 7(1) 104-13 https://www.ncbi.nlm.nih.gov/pubmed/9172774

1078 Schmid B, Krömer M, Schulz G E FEBS letters. Expression of porin from Rhodopseudomonas blastica in Escherichia coli inclusion bodies and folding into exact native structure. 1996 Feb: 381(1-2) 111-4 https://www.ncbi.nlm.nih.gov/pubmed/8641415

1079 Kumar T K, Yang P W, Lin S H, Wu C Y, Lei B, Lo S J, Tu S C, Yu C Biochemical and biophysical research communications. Cloning, direct expression, and purification of a snake venom cardiotoxin in Escherichia coli. 1996 Feb: 219(2) 450-6 https://www.ncbi.nlm.nih.gov/pubmed/8605008

1080 Lim B L, Holmskov U Biochemical and biophysical research communications. Expression of the carbohydrate recognition domain of bovine conglutinin and demonstration of its binding to iC3b and yeast mannan. 1996 Jan: 218(1) 260-6 https://www.ncbi.nlm.nih.gov/pubmed/8573143

1081 Castellanos-Serra L R, Hardy E, Ubieta R, Vispo N S, Fernandez C, Besada V, Falcon V, Gonzalez M, Santos A, Perez G, Silva A, Herrera L FEBS letters. Expression and folding of an interleukin-2-proinsulin fusion protein and its conversion into insulin by a single step enzymatic removal of the C-peptide and the N-terminal fused sequence. 1996 Jan: 378(2) 171-6 https://www.ncbi.nlm.nih.gov/pubmed/8549827

1082 Jair K W, Martin R G, Rosner J L, Fujita N, Ishihama A, Wolf R E Journal of bacteriology. Purification and regulatory properties of MarA protein, a transcriptional activator of Escherichia coli multiple antibiotic and superoxide resistance promoters. 1995 Dec: 177(24) 7100-4 https://www.ncbi.nlm.nih.gov/pubmed/8522515

1083 Boros I M, Tie F, Giam C Z Virology. Interaction of bovine leukemia virus transactivator Tax with bZip proteins. 1995 Dec: 214(1) 207-14 https://www.ncbi.nlm.nih.gov/pubmed/8525616

1084 Dodd I, Mossakowska D E, Camilleri P, Haran M, Hensley P, Lawlor E J, McBay D L, Pindar W, Smith R A Protein expression and purification. Overexpression in Escherichia coli, folding, purification, and characterization of the first three short consensus repeat modules of human complement receptor type 1. 1995 Dec: 6(6) 727-36 https://www.ncbi.nlm.nih.gov/pubmed/8746624

1085 Meng J, John T R, Kaiser I I Biochemical pharmacology. Specificity and binding affinity of an anti-crotoxin combinatorial antibody selected from a phage-displayed library. 1995 Dec: 50(12) 1969-77 https://www.ncbi.nlm.nih.gov/pubmed/8849322

1086 Sandowski Y, Nagano M, Bignon C, Djiane J, Kelly P A, Gertler A Molecular and cellular endocrinology. Preparation and characterization of recombinant prolactin receptor extracellular domain from rat. 1995 Nov: 115(1) 1-11 https://www.ncbi.nlm.nih.gov/pubmed/8674857

1087 Whitwam R E, Gazarian I G, Tien M Biochemical and biophysical research communications. Expression of fungal Mn peroxidase in E. coli and refolding to yield active enzyme. 1995 Nov: 216(3) 1013-7 https://www.ncbi.nlm.nih.gov/pubmed/7488173

1088 Sun L, Li M S, Fisher L M, Spry C J Protein expression and purification. Expression in Escherichia coli and purification of human eosinophil-derived neurotoxin with ribonuclease activity. 1995 Oct: 6(5) 685-92 https://www.ncbi.nlm.nih.gov/pubmed/8535163

1089 Frank L J, Wisniewski D, Hammond G G, Hermes J, Marcy A, Cameron P M Protein expression and purification. High-yield expression, refolding, and purification of penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus strain 27R. 1995 Oct: 6(5) 671-8 https://www.ncbi.nlm.nih.gov/pubmed/8535161

1090 Birch G M, Black T, Malcolm S K, Lai M T, Zimmerman R E, Jaskunas S R Protein expression and purification. Purification of recombinant human rhinovirus 14 3C protease expressed in Escherichia coli. 1995 Oct: 6(5) 609-18 https://www.ncbi.nlm.nih.gov/pubmed/8535153

1091 Yang J, Moyana T, Xiang J Molecular immunology. A genetically engineered single-chain FV/TNF molecule possesses the anti-tumor immunoreactivity of FV as well as the cytotoxic activity of tumor necrosis factor. 1995 Aug: 32(12) 873-81 https://www.ncbi.nlm.nih.gov/pubmed/7565814

1092 Ren P, Sairam M R, Yarney T A Molecular and cellular endocrinology. Bacterial expression of human chorionic gonadotropin alpha subunit: studies on refolding, dimer assembly and interaction with two different beta subunits. 1995 Aug: 113(1) 39-51 https://www.ncbi.nlm.nih.gov/pubmed/8674812

1093 Dolinar M, Maganja D B, Turk V Biological chemistry Hoppe-Seyler. Expression of full-length human procathepsin L cDNA in Escherichia coli and refolding of the expression product. 1995 Jun: 376(6) 385-8 https://www.ncbi.nlm.nih.gov/pubmed/7576233

1094 Calero M, Méndez E, García E Biochimica et biophysica acta. Expression of the human complex-forming glycoprotein HC (alpha 1-microglobulin) in Escherichia coli. 1995 May: 1249(1) 91-9 https://www.ncbi.nlm.nih.gov/pubmed/7539295

1095 Ramage P, Cheneval D, Chvei M, Graff P, Hemmig R, Heng R, Kocher H P, Mackenzie A, Memmert K, Revesz L The Journal of biological chemistry. Expression, refolding, and autocatalytic proteolytic processing of the interleukin-1 beta-converting enzyme precursor. 1995 Apr: 270(16) 9378-83 https://www.ncbi.nlm.nih.gov/pubmed/7721861

1096 Kuhelj R, Dolinar M, Pungercar J, Turk V European journal of biochemistry / FEBS. The preparation of catalytically active human cathepsin B from its precursor expressed in Escherichia coli in the form of inclusion bodies. 1995 Apr: 229(2) 533-9 https://www.ncbi.nlm.nih.gov/pubmed/7744077

1097 Levine A D, Rangwala S H, Horn N A, Peel M A, Matthews B K, Leimgruber R M, Manning J A, Bishop B F, Olins P O The Journal of biological chemistry. High level expression and refolding of mouse interleukin 4 synthesized in Escherichia coli. 1995 Mar: 270(13) 7445-52 https://www.ncbi.nlm.nih.gov/pubmed/7706290

1098 Chilkoti A, Tan P H, Stayton P S Proceedings of the National Academy of Sciences of the United States of America. Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120. 1995 Feb: 92(5) 1754-8 https://www.ncbi.nlm.nih.gov/pubmed/7878054

1099 Kupper D, Reuter M, Mackeldanz P, Meisel A, Alves J, Schroeder C, Krüger D H Protein expression and purification. Hyperexpressed EcoRII renatured from inclusion bodies and native enzyme both exhibit essential cooperativity with two DNA sites. 1995 Feb: 6(1) 1-9 https://www.ncbi.nlm.nih.gov/pubmed/7756833

1100 Koller G, Graumann K, Kramer W, Sara M, Jungbauer A Journal of chromatography. Laboratory-scale production and purification of recombinant HIV-1 reverse transcriptase. 1995 Feb: B, Biomedical applications. 664(1) 107-18 https://www.ncbi.nlm.nih.gov/pubmed/7538852

1101 Hellman J, Lassila P, Mäntsälä P Protein expression and purification. In vitro refolding of cyclomaltodextrin glucanotransferase from cytoplasmic inclusion bodies formed upon expression in Escherichia coli. 1995 Feb: 6(1) 56-62 https://www.ncbi.nlm.nih.gov/pubmed/7756839

1102 Lambalot R H, Cane D E, Aparicio J J, Katz L Biochemistry. Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK. 1995 Feb: 34(6) 1858-66 https://www.ncbi.nlm.nih.gov/pubmed/7849045

1103 Klimczak L J, Collinge M A, Farini D, Giuliano G, Walker J C, Cashmore A R The Plant cell. Reconstitution of Arabidopsis casein kinase II from recombinant subunits and phosphorylation of transcription factor GBF1. 1995 Jan: 7(1) 105-15 https://www.ncbi.nlm.nih.gov/pubmed/7696877

1104 DiBella E E, Maurer M C, Scheraga H A The Journal of biological chemistry. Expression and folding of recombinant bovine prethrombin-2 and its activation to thrombin. 1995 Jan: 270(1) 163-9 https://www.ncbi.nlm.nih.gov/pubmed/7814368

1105 Arimilli S, Cardoso C, Mukku P, Baichwal V, Nag B The Journal of biological chemistry. Refolding and reconstitution of functionally active complexes of human leukocyte antigen DR2 and myelin basic protein peptide from recombinant alpha and beta polypeptide chains. 1995 Jan: 270(2) 971-7 https://www.ncbi.nlm.nih.gov/pubmed/7529765

1106 Spencer E M, Chan K Progress in growth factor research. A 3-dimensional model for the insulin-like growth factor binding proteins (IGFBPs); supporting evidence using the structural determinants of the IGF binding site on IGFBP-3. 1995 : 6(2-4) 209-14 https://www.ncbi.nlm.nih.gov/pubmed/8817663

1107 Hale K K, Smith C G, Baker S L, Vanderslice R W, Squires C H, Gleason T M, Tucker K K, Kohno T, Russell D A Cytokine. Multifunctional regulation of the biological effects of TNF-alpha by the soluble type I and type II TNF receptors. 1995 Jan: 7(1) 26-38 https://www.ncbi.nlm.nih.gov/pubmed/7749064

1108 Levi S, Corsi B, Rovida E, Cozzi A, Santambrogio P, Albertini A, Arosio P The Journal of biological chemistry. Construction of a ferroxidase center in human ferritin L-chain. 1994 Dec: 269(48) 30334-9 https://www.ncbi.nlm.nih.gov/pubmed/7982945

1109 Goode D, Crabbe M J Archives of biochemistry and biophysics. Heterologous expression of gamma E-crystallin produces protein with an aberrant tertiary structure. 1994 Nov: 315(1) 104-10 https://www.ncbi.nlm.nih.gov/pubmed/7979386

1110 Gazaryan I G, Doseeva V V, Galkin A G, Tishkov V I FEBS letters. Effect of single-point mutations Phe41-->His and Phe143-->Glu on folding and catalytic properties of recombinant horseradish peroxidase expressed in E. coli. 1994 Nov: 354(3) 248-50 https://www.ncbi.nlm.nih.gov/pubmed/7957932

1111 Hilyard K L, Reyburn H, Chung S, Bell J I, Strominger J L Proceedings of the National Academy of Sciences of the United States of America. Binding of soluble natural ligands to a soluble human T-cell receptor fragment produced in Escherichia coli. 1994 Sep: 91(19) 9057-61 https://www.ncbi.nlm.nih.gov/pubmed/8090768

1112 Hill J, Tyas L, Phylip L H, Kay J, Dunn B M, Berry C FEBS letters. High level expression and characterisation of Plasmepsin II, an aspartic proteinase from Plasmodium falciparum. 1994 Sep: 352(2) 155-8 https://www.ncbi.nlm.nih.gov/pubmed/7925966

1113 Chao Q, Casalongue C, Quinn J M, Etzler M E Archives of biochemistry and biophysics. Expression and partial characterization of Dolichos biflorus seed lectin in Escherichia coli. 1994 Sep: 313(2) 346-50 https://www.ncbi.nlm.nih.gov/pubmed/8080282

1114 Nishiyama C, Yuuki T, Usui Y, Iwamoto N, Okumura Y, Okudaira H International archives of allergy and immunology. Effects of amino acid variations in recombinant Der fII on its human IgE and mouse IgG recognition. 1994 Sep: 105(1) 62-9 https://www.ncbi.nlm.nih.gov/pubmed/8086830

1115 Steinemann D, Lill H, Junge W, Engelbrecht S Biochimica et biophysica acta. Over-production, renaturation and reconstitution of delta and epsilon subunits from chloroplast and cyanobacterial F1. 1994 Sep: 1187(3) 354-9 https://www.ncbi.nlm.nih.gov/pubmed/7918533

1116 LoGrasso P V, Cornell-Kennon S, Boettcher B R The Journal of biological chemistry. Cloning, expression, and characterization of human apolipoprotein(a) kringle IV37. 1994 Aug: 269(34) 21820-7 https://www.ncbi.nlm.nih.gov/pubmed/8063825

1117 Hayashi T, Matsubara M, Nohara D, Kojima S, Miura K, Sakai T FEBS letters. Renaturation of the mature subtilisin BPN' immobilized on agarose beads. 1994 Aug: 350(1) 109-12 https://www.ncbi.nlm.nih.gov/pubmed/8062906

1118 Misawa S, Aoshima M, Takaku H, Matsumoto M, Hayashi H Journal of biotechnology. High-level expression of Mycoplasma arginine deiminase in Escherichia coli and its efficient renaturation as an anti-tumor enzyme. 1994 Aug: 36(2) 145-55 https://www.ncbi.nlm.nih.gov/pubmed/7765234

1119 Efimov V A, Fradkov A F, Raskind A B, Khristin M S, Klimov V V, Chakhmakhcheva O G FEBS letters. Expression of the barley psbA gene in Escherichia coli yields a functional in vitro photosystem II protein D1. 1994 Jul: 348(2) 153-7 https://www.ncbi.nlm.nih.gov/pubmed/8034031

1120 Qi H L, Tai J Y, Blake M S Infection and immunity. Expression of large amounts of neisserial porin proteins in Escherichia coli and refolding of the proteins into native trimers. 1994 Jun: 62(6) 2432-9 https://www.ncbi.nlm.nih.gov/pubmed/8188368

1121 Le K D, Gilkes N R, Kilburn D G, Miller R C, Saddler J N, Warren R A Enzyme and microbial technology. A streptavidin-cellulose-binding domain fusion protein that binds biotinylated proteins to cellulose. 1994 Jun: 16(6) 496-500 https://www.ncbi.nlm.nih.gov/pubmed/7764888

1122 Suttnar J, Dyr J E, Hamsíková E, Novák J, Vonka V Journal of chromatography. Procedure for refolding and purification of recombinant proteins from Escherichia coli inclusion bodies using a strong anion exchanger. 1994 Jun: B, Biomedical applications. 656(1) 123-6 https://www.ncbi.nlm.nih.gov/pubmed/7952022

1123 Holtet T L, Nielsen K L, Etzerodt M, Moestrup S K, Gliemann J, Sottrup-Jensen L, Thøgersen H C FEBS letters. Receptor-binding domain of human alpha 2-macroglobulin. Expression, folding and biochemical characterization of a high-affinity recombinant derivative. 1994 May: 344(2-3) 242-6 https://www.ncbi.nlm.nih.gov/pubmed/7514545

1124 Werner M H, Clore G M, Gronenborn A M, Kondoh A, Fisher R J FEBS letters. Refolding proteins by gel filtration chromatography. 1994 May: 345(2-3) 125-30 https://www.ncbi.nlm.nih.gov/pubmed/8200443

1125 Tanaka T, Hayashi M, Kimura H, Oobatake M, Nakamura H Biophysical chemistry. De novo design and creation of a stable artificial protein. 1994 May: 50(1-2) 47-61 https://www.ncbi.nlm.nih.gov/pubmed/8011940

1126 Panayotatos N, Everdeen D, Liten A, Somogyi R, Acheson A Biochemistry. Recombinant human CNTF receptor alpha: production, binding stoichiometry, and characterization of its activity as a diffusible factor. 1994 May: 33(19) 5813-8 https://www.ncbi.nlm.nih.gov/pubmed/8180210

1127 DeLoskey R J, Van Dyk D E, Van Aken T E, Campbell-Burk S Archives of biochemistry and biophysics. Isolation and refolding of H-ras from inclusion bodies of Escherichia coli: refold procedure and comparison of refolded and soluble H-ras. 1994 May: 311(1) 72-8 https://www.ncbi.nlm.nih.gov/pubmed/8185322

1128 Cho Y, Batt C A, Sawyer L The Journal of biological chemistry. Probing the retinol-binding site of bovine beta-lactoglobulin. 1994 Apr: 269(15) 11102-7 https://www.ncbi.nlm.nih.gov/pubmed/8157636

1129 Matsubara M, Kurimoto E, Kojima S, Miura K, Sakai T FEBS letters. Achievement of renaturation of subtilisin BPN' by a novel procedure using organic salts and a digestible mutant of Streptomyces subtilisin inhibitor. 1994 Apr: 342(2) 193-6 https://www.ncbi.nlm.nih.gov/pubmed/8143876

1130 Nag B, Arimilli S, Koukis B, Rhodes E, Baichwal V, Sharma S D The Journal of biological chemistry. Intramolecular charge heterogeneity in purified major histocompatibility class II alpha and beta polypeptide chains. 1994 Apr: 269(13) 10061-70 https://www.ncbi.nlm.nih.gov/pubmed/8144505

1131 Skelly S M, Tackney C, Hicklin D, Tamkins T, Goldstein N, Waksal H, Dagan S Journal of biotechnology. High-level expression of a biologically active human interleukin-6 mutein. 1994 Apr: 34(1) 79-86 https://www.ncbi.nlm.nih.gov/pubmed/7764745

1132 Kwon K S, Kim J, Shin H S, Yu M H The Journal of biological chemistry. Single amino acid substitutions of alpha 1-antitrypsin that confer enhancement in thermal stability. 1994 Apr: 269(13) 9627-31 https://www.ncbi.nlm.nih.gov/pubmed/8144550

1133 Diaz-Collier J A, Palmier M O, Kretzmer K K, Bishop B F, Combs R G, Obukowicz M G, Frazier R B, Bild G S, Joy W D, Hill S R Thrombosis and haemostasis. Refold and characterization of recombinant tissue factor pathway inhibitor expressed in Escherichia coli. 1994 Mar: 71(3) 339-46 https://www.ncbi.nlm.nih.gov/pubmed/8029799

1134 Tanaka T, Kimura H, Hayashi M, Fujiyoshi Y, Fukuhara K, Nakamura H Protein science : a publication of the Protein Society. Characteristics of a de novo designed protein. 1994 Mar: 3(3) 419-27 https://www.ncbi.nlm.nih.gov/pubmed/8019413

1135 Moser M, Menz G, Blaser K, Crameri R Infection and immunity. Recombinant expression and antigenic properties of a 32-kilodalton extracellular alkaline protease, representing a possible virulence factor from Aspergillus fumigatus. 1994 Mar: 62(3) 936-42 https://www.ncbi.nlm.nih.gov/pubmed/8112866

1136 Hibino T, Misawa S, Wakiyama M, Maeda S, Yazaki K, Kumagai I, Ooi T, Miura K Journal of biotechnology. High-level expression of porcine muscle adenylate kinase in Escherichia coli: effects of the copy number of the gene and the translational initiation signals. 1994 Feb: 32(2) 139-48 https://www.ncbi.nlm.nih.gov/pubmed/7764560

1137 Li Y, Inouye M The Journal of biological chemistry. Autoprocessing of prothiolsubtilisin E in which active-site serine 221 is altered to cysteine. 1994 Feb: 269(6) 4169-74 https://www.ncbi.nlm.nih.gov/pubmed/8307978

1138 Fisher R J, Fivash M, Casas-Finet J, Erickson J W, Kondoh A, Bladen S V, Fisher C, Watson D K, Papas T Protein science : a publication of the Protein Society. Real-time DNA binding measurements of the ETS1 recombinant oncoproteins reveal significant kinetic differences between the p42 and p51 isoforms. 1994 Feb: 3(2) 257-66 https://www.ncbi.nlm.nih.gov/pubmed/8003962

1139 Hamamoto T, Lee Y C, Kurosawa N, Nakaoka T, Kojima N, Tsuji S Bioorganic & medicinal chemistry. Expression of mouse Gal beta 1,4GlcNAc alpha 2,6-sialyltransferase in an insoluble form in Escherichia coli and partial renaturation. 1994 Feb: 2(2) 79-84 https://www.ncbi.nlm.nih.gov/pubmed/7922126

1140 Bignon C, Sakal E, Belair L, Chapnik-Cohen N, Djiane J, Gertler A The Journal of biological chemistry. Preparation of the extracellular domain of the rabbit prolactin receptor expressed in Escherichia coli and its interaction with lactogenic hormones. 1994 Feb: 269(5) 3318-24 https://www.ncbi.nlm.nih.gov/pubmed/8106371

1141 Seddon A P, Hulmes J D, Decker M M, Kovesdi I, Fairhurst J L, Backer J, Dougher-Vermazen M, Böhlen P Protein expression and purification. Refolding and characterization of human recombinant heparin-binding neurite-promoting factor. 1994 Feb: 5(1) 14-21 https://www.ncbi.nlm.nih.gov/pubmed/8167469

1142 Jin H, Uddin M S, Huang Y L, Teo W K Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986). Purification and renaturation of recombinant human lymphotoxin (tumour necrosis factor beta) expressed in Escherichia coli as inclusion bodies. 1994 Jan: 59(1) 67-72 https://www.ncbi.nlm.nih.gov/pubmed/7764497

1143 Xia H, Wang Y, Sun J Chinese journal of biotechnology. Characterization of polyketide ketoreductase gene (MPKR) from midecamycin-producing strain (Streptomyces mycarofaciens 1748). 1994 : 10(3) 169-78 https://www.ncbi.nlm.nih.gov/pubmed/7893937

1144 Fandl J P, Tobkes N J, McDonald N Q, Hendrickson W A, Ryan T E, Nigam S, Acheson A, Cudny H, Panayotatos N The Journal of biological chemistry. Characterization and crystallization of recombinant human neurotrophin-4. 1994 Jan: 269(1) 755-9 https://www.ncbi.nlm.nih.gov/pubmed/8276879

1145 Kleine T, Bartsch S, Bläser J, Schnierer S, Triebel S, Valentin M, Gote T, Tschesche H Biochemistry. Preparation of active recombinant TIMP-1 from Escherichia coli inclusion bodies and complex formation with the recombinant catalytic domain of PMNL-collagenase. 1993 Dec: 32(51) 14125-31 https://www.ncbi.nlm.nih.gov/pubmed/8260495

1146 Lubega G W, Geary T G, Klein R D, Prichard R K Molecular and biochemical parasitology. Expression of cloned beta-tubulin genes of Haemonchus contortus in Escherichia coli: interaction of recombinant beta-tubulin with native tubulin and mebendazole. 1993 Dec: 62(2) 281-92 https://www.ncbi.nlm.nih.gov/pubmed/8139621

1147 Wong K K, Murray B W, Lewisch S A, Baxter M K, Ridky T W, Ulissi-DeMario L, Kozarich J W Biochemistry. Molecular properties of pyruvate formate-lyase activating enzyme. 1993 Dec: 32(51) 14102-10 https://www.ncbi.nlm.nih.gov/pubmed/8260492

1148 Liang N S, Pungercar J, Krizaj I, Strukelj B, Gubensek F FEBS letters. Expression of fully active ammodytoxin A, a potent presynaptically neurotoxic phospholipase A2, in Escherichia coli. 1993 Nov: 334(1) 55-9 https://www.ncbi.nlm.nih.gov/pubmed/8224227

1149 Altman J D, Reay P A, Davis M M Proceedings of the National Academy of Sciences of the United States of America. Formation of functional peptide complexes of class II major histocompatibility complex proteins from subunits produced in Escherichia coli. 1993 Nov: 90(21) 10330-4 https://www.ncbi.nlm.nih.gov/pubmed/8234294

1150 Becerra S P, Palmer I, Kumar A, Steele F, Shiloach J, Notario V, Chader G J The Journal of biological chemistry. Overexpression of fetal human pigment epithelium-derived factor in Escherichia coli. A functionally active neurotrophic factor. 1993 Nov: 268(31) 23148-56 https://www.ncbi.nlm.nih.gov/pubmed/8226833

1151 Sirawaraporn W, Prapunwattana P, Sirawaraporn R, Yuthavong Y, Santi D V The Journal of biological chemistry. The dihydrofolate reductase domain of Plasmodium falciparum thymidylate synthase-dihydrofolate reductase. Gene synthesis, expression, and anti-folate-resistant mutants. 1993 Oct: 268(29) 21637-44 https://www.ncbi.nlm.nih.gov/pubmed/8408015

1152 Lin X, Tang J, Koelsch G, Monod M, Foundling S The Journal of biological chemistry. Recombinant canditropsin, an extracellular aspartic protease from yeast Candida tropicalis. Escherichia coli expression, purification, zymogen activation, and enzymic properties. 1993 Sep: 268(27) 20143-7 https://www.ncbi.nlm.nih.gov/pubmed/8376373

1153 Bai M, Harfe B, Freimuth P Journal of virology. Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells. 1993 Sep: 67(9) 5198-205 https://www.ncbi.nlm.nih.gov/pubmed/8350395

1154 Fiermonte G, Walker J E, Palmieri F The Biochemical journal. Abundant bacterial expression and reconstitution of an intrinsic membrane-transport protein from bovine mitochondria. 1993 Aug: 294 ( Pt 1) 293-9 https://www.ncbi.nlm.nih.gov/pubmed/8363582

1155 Leuthardt A, Roesel J L FEBS letters. Cloning, expression and purification of a recombinant poly-histidine-linked HIV-1 protease. 1993 Jul: 326(1-3) 275-80 https://www.ncbi.nlm.nih.gov/pubmed/8325379

1156 Lin W J, Traugh J A Protein expression and purification. Renaturation of casein kinase II from recombinant subunits produced in Escherichia coli: purification and characterization of the reconstituted holoenzyme. 1993 Jun: 4(3) 256-64 https://www.ncbi.nlm.nih.gov/pubmed/8390882

1157 Gött P, Stohwasser R, Schnitzler P, Darai G, Bautz E K Virology. RNA binding of recombinant nucleocapsid proteins of hantaviruses. 1993 May: 194(1) 332-7 https://www.ncbi.nlm.nih.gov/pubmed/8480424

1158 Kurucz I, Jost C R, George A J, Andrew S M, Segal D M Proceedings of the National Academy of Sciences of the United States of America. A bacterially expressed single-chain Fv construct from the 2B4 T-cell receptor. 1993 May: 90(9) 3830-4 https://www.ncbi.nlm.nih.gov/pubmed/8387198

1159 Hulmes J D, Seddon A P, Decker M M, Böhlen P Biochemical and biophysical research communications. Comparison of the disulfide bond arrangements of human recombinant and bovine brain heparin binding neurite-promoting factors. 1993 Apr: 192(2) 738-46 https://www.ncbi.nlm.nih.gov/pubmed/8484780

1160 Prestwich G D Protein science : a publication of the Protein Society. Bacterial expression and photoaffinity labeling of a pheromone binding protein. 1993 Mar: 2(3) 420-8 https://www.ncbi.nlm.nih.gov/pubmed/8453379

1161 Lu W P, Schiau I, Cunningham J R, Ragsdale S W The Journal of biological chemistry. Sequence and expression of the gene encoding the corrinoid/iron-sulfur protein from Clostridium thermoaceticum and reconstitution of the recombinant protein to full activity. 1993 Mar: 268(8) 5605-14 https://www.ncbi.nlm.nih.gov/pubmed/8449924

1162 Wilkins J A, Cone J, Randhawa Z I, Wood D, Warren M K, Witkowska H E Protein science : a publication of the Protein Society. A study of intermediates involved in the folding pathway for recombinant human macrophage colony-stimulating factor (M-CSF): evidence for two distinct folding pathways. 1993 Feb: 2(2) 244-54 https://www.ncbi.nlm.nih.gov/pubmed/8443602

1163 Chen W, Bahl O P Molecular and cellular endocrinology. High expression of the hormone binding active extracellular domain (1-294) of rat lutropin receptor in Escherichia coli. 1993 Feb: 91(1-2) 35-41 https://www.ncbi.nlm.nih.gov/pubmed/8472852

1164 Delves C J, Ballantine S P, Tansik R L, Baccanari D P, Stammers D K Protein expression and purification. Refolding of recombinant Pneumocystis carinii dihydrofolate reductase and characterization of the enzyme. 1993 Feb: 4(1) 16-23 https://www.ncbi.nlm.nih.gov/pubmed/8425104

1165 Jonsson C B, Donzella G A, Roth M J The Journal of biological chemistry. Characterization of the forward and reverse integration reactions of the Moloney murine leukemia virus integrase protein purified from Escherichia coli. 1993 Jan: 268(2) 1462-9 https://www.ncbi.nlm.nih.gov/pubmed/8419346

1166 Fine M, Sakal E, Vashdi D, Daniel V, Levanon A, Lipshitz O, Gertler A General and comparative endocrinology. Recombinant carp (Cyprinus carpio) growth hormone: expression, purification, and determination of biological activity in vitro and in vivo. 1993 Jan: 89(1) 51-61 https://www.ncbi.nlm.nih.gov/pubmed/8428649

1167 Brown W C, Duncan J A, Campbell J L The Journal of biological chemistry. Purification and characterization of the Saccharomyces cerevisiae DNA polymerase delta overproduced in Escherichia coli. 1993 Jan: 268(2) 982-90 https://www.ncbi.nlm.nih.gov/pubmed/8380419

1168 Day K C, Welsch D J Thrombosis research. Bacterial expression, purification, and partial characterization of amino acids 94-155 of human tissue factor pathway inhibitor (TFPI) as an inhibitor of blood coagulation factor Xa. 1992 Dec: 68(4-5) 369-81 https://www.ncbi.nlm.nih.gov/pubmed/1290165

1169 Ye Q Z, Johnson L L, Hupe D J, Baragi V Biochemistry. Purification and characterization of the human stromelysin catalytic domain expressed in Escherichia coli. 1992 Nov: 31(45) 11231-5 https://www.ncbi.nlm.nih.gov/pubmed/1445862

1170 Lin X, Liu M, Tang J Enzyme and microbial technology. Heterologous expression of thermopsin, a heat-stable acid proteinase. 1992 Sep: 14(9) 696-701 https://www.ncbi.nlm.nih.gov/pubmed/1368892

1171 Cocuzzi E T, Walther S E, Rajan S, Denhardt D T FEBS letters. Expression and purification of mouse TIMP-1 from E. coli. 1992 Aug: 307(3) 375-8 https://www.ncbi.nlm.nih.gov/pubmed/1322839

1172 Moser M, Crameri R, Menz G, Schneider T, Dudler T, Virchow C, Gmachl M, Blaser K, Suter M Journal of immunology (Baltimore, Md. Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp f I/a) with IgE binding and type I skin test activity. 1992 Jul: : 1950). 149(2) 454-60 https://www.ncbi.nlm.nih.gov/pubmed/1624793

1173 Gertler A, Hauser S D, Sakal E, Vashdi D, Staten N, Freeman J J, Krivi G G The Journal of biological chemistry. Preparation, purification, and determination of the biological activities of 12 N terminus-truncated recombinant analogues of bovine placental lactogen. 1992 Jun: 267(18) 12655-9 https://www.ncbi.nlm.nih.gov/pubmed/1618771

1174 Sawano H, Koumoto Y, Ohta K, Sasaki Y, Segawa S, Tachibana H FEBS letters. Efficient in vitro folding of the three-disulfide derivatives of hen lysozyme in the presence of glycerol. 1992 May: 303(1) 11-4 https://www.ncbi.nlm.nih.gov/pubmed/1592107

1175 Schlunegger M P, Cerletti N, Cox D A, McMaster G K, Schmitz A, Grütter M G FEBS letters. Crystallization and preliminary X-ray analysis of recombinant human transforming growth factor beta 2. 1992 May: 303(1) 91-3 https://www.ncbi.nlm.nih.gov/pubmed/1592122

1176 Garboczi D N, Hung D T, Wiley D C Proceedings of the National Academy of Sciences of the United States of America. HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides. 1992 Apr: 89(8) 3429-33 https://www.ncbi.nlm.nih.gov/pubmed/1565634

1177 Edwards G M, Huber H E, DeFeo-Jones D, Vuocolo G, Goodhart P J, Maigetter R Z, Sanyal G, Oliff A, Heimbrook D C The Journal of biological chemistry. Purification and characterization of a functionally homogeneous 60-kDa species of the retinoblastoma gene product. 1992 Apr: 267(12) 7971-4 https://www.ncbi.nlm.nih.gov/pubmed/1569054

1178 Conner G E, Richo G Biochemistry. Isolation and characterization of a stable activation intermediate of the lysosomal aspartyl protease cathepsin D. 1992 Feb: 31(4) 1142-7 https://www.ncbi.nlm.nih.gov/pubmed/1734961

1179 Kawase Y, Ohdate Y, Shimojo T, Taguchi Y, Kimizuka F, Kato I FEBS letters. Construction and characterization of a fusion protein with epidermal growth factor and the cell-binding domain of fibronectin. 1992 Feb: 298(2-3) 126-8 https://www.ncbi.nlm.nih.gov/pubmed/1544433

1180 Chen Z, Wu I, Richter M L, Gegenheimer P FEBS letters. Over-expression and refolding of beta-subunit from the chloroplast ATP synthase. 1992 Feb: 298(1) 69-73 https://www.ncbi.nlm.nih.gov/pubmed/1531962

1181 Calalb M B, Fox D T, Hanks S K The Journal of biological chemistry. Molecular cloning and enzymatic analysis of the rat homolog of "PhK-gamma T," an isoform of phosphorylase kinase catalytic subunit. 1992 Jan: 267(3) 1455-63 https://www.ncbi.nlm.nih.gov/pubmed/1370475

1182 Kelley M J, Crowl R M, Dennis E A Biochimica et biophysica acta. Renaturation of cobra venom phospholipase A2 expressed from a synthetic gene in Escherichia coli. 1992 Jan: 1118(2) 107-15 https://www.ncbi.nlm.nih.gov/pubmed/1730025

1183 Landman O, Shiffman D, Av-Gay Y, Aharonowitz Y, Cohen G FEMS microbiology letters. High level expression in Escherichia coli of isopenicillin N synthase genes from Flavobacterium and Streptomyces, and recovery of active enzyme from inclusion bodies. 1991 Dec: 68(3) 239-44 https://www.ncbi.nlm.nih.gov/pubmed/1666621

1184 Feldmar S, Kunze R The EMBO journal. The ORFa protein, the putative transposase of maize transposable element Ac, has a basic DNA binding domain. 1991 Dec: 10(13) 4003-10 https://www.ncbi.nlm.nih.gov/pubmed/1661668

1185 Puri N K FEBS letters. Refolding of recombinant porcine growth hormone in a reducing environment limits in vitro aggregate formation. 1991 Nov: 292(1-2) 187-90 https://www.ncbi.nlm.nih.gov/pubmed/1959605

1186 Swennen D, Rentier-Delrue F, Auperin B, Prunet P, Flik G, Wendelaar Bonga S E, Lion M, Martial J A The Journal of endocrinology. Production and purification of biologically active recombinant tilapia (Oreochromis niloticus) prolactins. 1991 Nov: 131(2) 219-27 https://www.ncbi.nlm.nih.gov/pubmed/1744569

1187 Novotny J, Ganju R K, Smiley S T, Hussey R E, Luther M A, Recny M A, Siliciano R F, Reinherz E L Proceedings of the National Academy of Sciences of the United States of America. A soluble, single-chain T-cell receptor fragment endowed with antigen-combining properties. 1991 Oct: 88(19) 8646-50 https://www.ncbi.nlm.nih.gov/pubmed/1924326

1188 Harper J W, Logsdon N J Biochemistry. Refolded HIV-1 tat protein protects both bulge and loop nucleotides in TAR RNA from ribonucleolytic cleavage. 1991 Aug: 30(32) 8060-6 https://www.ncbi.nlm.nih.gov/pubmed/1868081

1189 Denzin L K, Whitlow M, Voss E W The Journal of biological chemistry. Single-chain site-specific mutations of fluorescein-amino acid contact residues in high affinity monoclonal antibody 4-4-20. 1991 Jul: 266(21) 14095-103 https://www.ncbi.nlm.nih.gov/pubmed/1856233

1190 Claassen L A, Ahn B, Koo H S, Grossman L The Journal of biological chemistry. Construction of deletion mutants of the Escherichia coli UvrA protein and their purification from inclusion bodies. 1991 Jun: 266(17) 11380-7 https://www.ncbi.nlm.nih.gov/pubmed/1645734

1191 Chen Z, Koelsch G, Han H P, Wang X J, Lin X L, Hartsuck J A, Tang J The Journal of biological chemistry. Recombinant rhizopuspepsinogen. Expression, purification, and activation properties of recombinant rhizopuspepsinogens. 1991 Jun: 266(18) 11718-25 https://www.ncbi.nlm.nih.gov/pubmed/2050673

1192 Tame J, Shih D T, Pagnier J, Fermi G, Nagai K Journal of molecular biology. Functional role of the distal valine (E11) residue of alpha subunits in human haemoglobin. 1991 Apr: 218(4) 761-7 https://www.ncbi.nlm.nih.gov/pubmed/2023247

1193 Orsini G, Brandazza A, Sarmientos P, Molinari A, Lansen J, Cauet G European journal of biochemistry / FEBS. Efficient renaturation and fibrinolytic properties of prourokinase and a deletion mutant expressed in Escherichia coli as inclusion bodies. 1991 Feb: 195(3) 691-7 https://www.ncbi.nlm.nih.gov/pubmed/1847867

1194 Cheng Y S, Yin F H, Foundling S, Blomstrom D, Kettner C A Proceedings of the National Academy of Sciences of the United States of America. Stability and activity of human immunodeficiency virus protease: comparison of the natural dimer with a homologous, single-chain tethered dimer. 1990 Dec: 87(24) 9660-4 https://www.ncbi.nlm.nih.gov/pubmed/2263618

1195 Dornmair K, Kiefer H, Jähnig F The Journal of biological chemistry. Refolding of an integral membrane protein. OmpA of Escherichia coli. 1990 Nov: 265(31) 18907-11 https://www.ncbi.nlm.nih.gov/pubmed/2229053

1196 Babbitt P C, West B L, Buechter D D, Kuntz I D, Kenyon G L Bio/technology (Nature Publishing Company). Removal of a proteolytic activity associated with aggregates formed from expression of creatine kinase in Escherichia coli leads to improved recovery of active enzyme. 1990 Oct: 8(10) 945-9 https://www.ncbi.nlm.nih.gov/pubmed/1370005

1197 Einberger H, Mertz R, Hofschneider P H, Neubert W J Journal of virology. Purification, renaturation, and reconstituted protein kinase activity of the Sendai virus large (L) protein: L protein phosphorylates the NP and P proteins in vitro. 1990 Sep: 64(9) 4274-80 https://www.ncbi.nlm.nih.gov/pubmed/2166816

1198 Kuwahara J, Coleman J E Biochemistry. Role of the zinc(II) ions in the structure of the three-finger DNA binding domain of the Sp1 transcription factor. 1990 Sep: 29(37) 8627-31 https://www.ncbi.nlm.nih.gov/pubmed/2271546

1199 Fountoulakis M, Juranville J F, Stüber D, Weibel E K, Garotta G The Journal of biological chemistry. Purification and biochemical characterization of a soluble human interferon gamma receptor expressed in Escherichia coli. 1990 Aug: 265(22) 13268-75 https://www.ncbi.nlm.nih.gov/pubmed/2142945

1200 Matsuyama S, Kimura E, Mizushima S The Journal of biological chemistry. Complementation of two overlapping fragments of SecA, a protein translocation ATPase of Escherichia coli, allows ATP binding to its amino-terminal region. 1990 May: 265(15) 8760-5 https://www.ncbi.nlm.nih.gov/pubmed/2160468

1201 Hagen A J, Hatton T A, Wang D I Biotechnology and bioengineering. Protein refolding in reversed micelles. 1990 Apr: 35(10) 955-65 https://www.ncbi.nlm.nih.gov/pubmed/18588240

1202 Cheng Y S, McGowan M H, Kettner C A, Schloss J V, Erickson-Viitanen S, Yin F H Gene. High-level synthesis of recombinant HIV-1 protease and the recovery of active enzyme from inclusion bodies. 1990 Mar: 87(2) 243-8 https://www.ncbi.nlm.nih.gov/pubmed/2158928

1203 Jin S G, Roitsch T, Christie P J, Nester E W Journal of bacteriology. The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes. 1990 Feb: 172(2) 531-7 https://www.ncbi.nlm.nih.gov/pubmed/2404941

1204 Tomasselli A G, Olsen M K, Hui J O, Staples D J, Sawyer T K, Heinrikson R L, Tomich C S Biochemistry. Substrate analogue inhibition and active site titration of purified recombinant HIV-1 protease. 1990 Jan: 29(1) 264-9 https://www.ncbi.nlm.nih.gov/pubmed/2182116

1205 Smith S M, Gottesman M M The Journal of biological chemistry. Activity and deletion analysis of recombinant human cathepsin L expressed in Escherichia coli. 1989 Dec: 264(34) 20487-95 https://www.ncbi.nlm.nih.gov/pubmed/2684978

1206 Wang L F, Hum W T, Kalyan N K, Lee S G, Hung P P, Doi R H Gene. Synthesis and refolding of human tissue-type plasminogen activator in Bacillus subtilis. 1989 Dec: 84(1) 127-33 https://www.ncbi.nlm.nih.gov/pubmed/2514121

1207 Howell M L, Blumenthal K M The Journal of biological chemistry. Cloning and expression of a synthetic gene for Cerebratulus lacteus neurotoxin B-IV. 1989 Sep: 264(26) 15268-73 https://www.ncbi.nlm.nih.gov/pubmed/2670934

1208 Ikehata O, Nishiyama M, Horinouchi S, Beppu T European journal of biochemistry / FEBS. Primary structure of nitrile hydratase deduced from the nucleotide sequence of a Rhodococcus species and its expression in Escherichia coli. 1989 May: 181(3) 563-70 https://www.ncbi.nlm.nih.gov/pubmed/2659343

1209 Chao B H, Costopoulos D S, Curiel T, Bertonis J M, Chisholm P, Williams C, Schooley R T, Rosa J J, Fisher R A, Maraganore J M The Journal of biological chemistry. A 113-amino acid fragment of CD4 produced in Escherichia coli blocks human immunodeficiency virus-induced cell fusion. 1989 Apr: 264(10) 5812-7 https://www.ncbi.nlm.nih.gov/pubmed/2647726

1210 Lin X L, Wong R N, Tang J The Journal of biological chemistry. Synthesis, purification, and active site mutagenesis of recombinant porcine pepsinogen. 1989 Mar: 264(8) 4482-9 https://www.ncbi.nlm.nih.gov/pubmed/2494172

1211 Thiele U, Auerswald E A, Gebhard W, Assfalg-Machleidt I, Popović T, Machleidt W Biological chemistry Hoppe-Seyler. Inhibitorily active recombinant human stefin B. Gene synthesis, expression and isolation of an inhibitory active MS-2 pol-stefin B fusion protein and preparation of Des[Met1,2(2)]stefin B. 1988 Oct: 369(10) 1167-78 https://www.ncbi.nlm.nih.gov/pubmed/2853948

1212 van Kimmenade A, Bond M W, Schumacher J H, Laquoi C, Kastelein R A European journal of biochemistry / FEBS. Expression, renaturation and purification of recombinant human interleukin 4 from Escherichia coli. 1988 Apr: 173(1) 109-14 https://www.ncbi.nlm.nih.gov/pubmed/3281831

1213 Tsuji T, Nakagawa R, Sugimoto N, Fukuhara K Biochemistry. Characterization of disulfide bonds in recombinant proteins: reduced human interleukin 2 in inclusion bodies and its oxidative refolding. 1987 Jun: 26(11) 3129-34 https://www.ncbi.nlm.nih.gov/pubmed/3038177

1214 Davis J M, Narachi M A, Alton N K, Arakawa T Biochemistry. Structure of human tumor necrosis factor alpha derived from recombinant DNA. 1987 Mar: 26(5) 1322-6 https://www.ncbi.nlm.nih.gov/pubmed/3552045

1215 Wilson M E, Miller L K Virology. Changes in the nucleoprotein complexes of a baculovirus DNA during infection. 1986 Jun: 151(2) 315-28 https://www.ncbi.nlm.nih.gov/pubmed/3518228

1216 Arakawa T, Alton N K, Hsu Y R The Journal of biological chemistry. Preparation and characterization of recombinant DNA-derived human interferon-gamma. 1985 Nov: 260(27) 14435-9 https://www.ncbi.nlm.nih.gov/pubmed/3932354

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1218 Gao Yong-Gui, Guan Yi-Xin, Yao Shan-Jing, Cho Man-Gi Biotechnology progress. On-column refolding of recombinant human interferon-gamma with an immobilized chaperone fragment. : 19(3) 915-20 https://www.ncbi.nlm.nih.gov/pubmed/12790657

1219 Anders R F, Crewther P E, Edwards S, Margetts M, Matthew M L, Pollock B, Pye D Vaccine. Immunisation with recombinant AMA-1 protects mice against infection with Plasmodium chabaudi. : 16(2-3) 240-7 https://www.ncbi.nlm.nih.gov/pubmed/9607037

1220 Saito Y, Ishii Y, Sasaki H, Hayashi M, Fujimura T, Imai Y, Nakamura S, Suzuki S, Notani J, Asada T Biotechnology progress. Production and characterization of a novel tissue-type plasminogen activator derivative in Escherichia coli. : 10(5) 472-9 https://www.ncbi.nlm.nih.gov/pubmed/7765373

1221 Musacchio A, Carmenate T, Delgado M, González S Vaccine. Recombinant Opc meningococcal protein, folded in vitro, elicits bactericidal antibodies after immunization. : 15(6-7) 751-8 https://www.ncbi.nlm.nih.gov/pubmed/9178478

1222 Yamaguchi Satoshi, Yamamoto Etsushi, Tsukiji Shinya, Nagamune Teruyuki Biotechnology progress. Successful control of aggregation and folding rates during refolding of denatured lysozyme by adding N-methylimidazolium cations with various N'-substituents. : 24(2) 402-8 https://www.ncbi.nlm.nih.gov/pubmed/18197673

1223 St John Richard J, Carpenter John F, Randolph Theodore W Biotechnology progress. High-pressure refolding of disulfide-cross-linked lysozyme aggregates: thermodynamics and optimization. : 18(3) 565-71 https://www.ncbi.nlm.nih.gov/pubmed/12052074

1224 Lubega George W, Ochola Donosian O K, Prichard Roger K Experimental parasitology. Trypanosoma brucei: anti-tubulin antibodies specifically inhibit trypanosome growth in culture. : 102(3-4) 134-42 https://www.ncbi.nlm.nih.gov/pubmed/12856308

1225 Doherty Daniel H, Rosendahl Mary S, Smith Darin J, Hughes Jennifer M, Chlipala Elizabeth A, Cox George N Bioconjugate chemistry. Site-specific PEGylation of engineered cysteine analogues of recombinant human granulocyte-macrophage colony-stimulating factor. : 16(5) 1291-8 https://www.ncbi.nlm.nih.gov/pubmed/16173810

1226 Gerardi Gianmario, Biasiotto Giorgio, Santambrogio Paolo, Zanella Isabella, Ingrassia Rosaria, Corrado Marcella, Cavadini Patrizia, Derosas Manuela, Levi Sonia, Arosio Paolo Blood cells, molecules & diseases. Recombinant human hepcidin expressed in Escherichia coli isolates as an iron containing protein. : 35(2) 177-81 https://www.ncbi.nlm.nih.gov/pubmed/16009582

1227 Goto M, Hashimoto Y, Fujita T, Ono T, Furusaki S Biotechnology progress. Important parameters affecting efficiency of protein refolding by reversed micelles. : 16(6) 1079-85 https://www.ncbi.nlm.nih.gov/pubmed/11101337

1228 Khan R H, Rao K B, Eshwari A N, Totey S M, Panda A K Biotechnology progress. Solubilization of recombinant ovine growth hormone with retention of native-like secondary structure and its refolding from the inclusion bodies of Escherichia coli. : 14(5) 722-8 https://www.ncbi.nlm.nih.gov/pubmed/9758661

1229 Kweon Dae-Hyuk, Lee Dae-Hee, Han Nam-Soo, Seo Jin-Ho Biotechnology progress. Solid-phase refolding of cyclodextrin glycosyltransferase adsorbed on cation-exchange resin. : 20(1) 277-83 https://www.ncbi.nlm.nih.gov/pubmed/14763853

1230 Wang Chaozhan, Wang Lili, Geng Xindu Biotechnology progress. High recovery refolding of rhG-CSF from Escherichia coli, using urea gradient size exclusion chromatography. : 24(1) 209-13 https://www.ncbi.nlm.nih.gov/pubmed/18179225

1231 Lanckriet Heikki, Middelberg Anton P J Biotechnology progress. Operational regimes for a simplified one-step artificial chaperone refolding method. : 20(6) 1861-7 https://www.ncbi.nlm.nih.gov/pubmed/15575723

1232 Xu Li-Ren, Yan Xiao, Luo Man, Guan Yi-Xin, Yao Shan-Jing Biotechnology progress. Preparation, characterization and refolding in vitro of a recombinant human cyclophilin A mutant: effect of a single Pro/Ser substitution on cyclophilin A structure and properties. : 24(2) 302-10 https://www.ncbi.nlm.nih.gov/pubmed/18333624